st034309

6-methoxy-2-(trichloromethyl)chromen-4-one

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1727
Screen concentration 9.9 μM
Source TimTec (Natural product derivative library)
PubChem CID 870305
SMILES COC1=CC2=C(C=C1)OC(=CC2=O)C(Cl)(Cl)Cl
Standardized SMILES COc1ccc2OC(=CC(=O)c2c1)C(Cl)(Cl)Cl
Molecular weight 293.5305
ALogP 3.13
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 16.58
% growth inhibition (Hom. pool) 9.89


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 870305
Download HIP data (tab-delimited text)  (excel)
Gene:ALA1(YOR335C)|FD-Score:3.97|P-value:3.61E-5|Clearance:0.14||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:ALG2(YGL065C)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.07||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:BET2(YPR176C)|FD-Score:5.76|P-value:4.13E-9|Clearance:0.45||SGD DESC:Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:BET4(YJL031C)|FD-Score:5.48|P-value:2.18E-8|Clearance:0.45||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:BRR6(YGL247W)|FD-Score:5.78|P-value:3.84E-9|Clearance:0.45||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:COG2(YGR120C)|FD-Score:3.82|P-value:6.62E-5|Clearance:0.08||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:FAL1(YDR021W)|FD-Score:4.39|P-value:5.63E-6|Clearance:0.13||SGD DESC:Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases Gene:FRQ1(YDR373W)|FD-Score:3.24|P-value:5.92E-4|Clearance:0.01||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:GFA1(YKL104C)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication Gene:GLC7(YER133W)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.08||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GLE1(YDL207W)|FD-Score:3.65|P-value:1.33E-4|Clearance:0.13||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:LSM2(YBL026W)|FD-Score:5.33|P-value:4.87E-8|Clearance:0.45||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MAS1(YLR163C)|FD-Score:3.74|P-value:9.15E-5|Clearance:0.09||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MCD4(YKL165C)|FD-Score:4.26|P-value:1.03E-5|Clearance:0.09||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MYO2(YOR326W)|FD-Score:5.99|P-value:1.05E-9|Clearance:0.45||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication Gene:NOB1(YOR056C)|FD-Score:5.41|P-value:3.19E-8|Clearance:0.45||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:NOP56(YLR197W)|FD-Score:4.88|P-value:5.29E-7|Clearance:0.23||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NSL1(YPL233W)|FD-Score:-3.37|P-value:3.72E-4|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NUF2(YOL069W)|FD-Score:4.65|P-value:1.65E-6|Clearance:0.26||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:NUG1(YER006W)|FD-Score:-4.64|P-value:1.75E-6|Clearance:0||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:RER2(YBR002C)|FD-Score:3.35|P-value:4.08E-4|Clearance:0.1||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RHO1(YPR165W)|FD-Score:3.52|P-value:2.15E-4|Clearance:0.04||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPL15A(YLR029C)|FD-Score:-3.73|P-value:9.53E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPN3(YER021W)|FD-Score:4.65|P-value:1.63E-6|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN6(YDL097C)|FD-Score:4.13|P-value:1.79E-5|Clearance:0.16||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RSC58(YLR033W)|FD-Score:3.83|P-value:6.49E-5|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:3.21|P-value:6.68E-4|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC6(YIL068C)|FD-Score:-5.26|P-value:7.38E-8|Clearance:0||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SEN1(YLR430W)|FD-Score:3.48|P-value:2.49E-4|Clearance:0.02||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SFH1(YLR321C)|FD-Score:3.39|P-value:3.55E-4|Clearance:0.04||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SLD2(YKL108W)|FD-Score:3.11|P-value:9.49E-4|Clearance:0.09||SGD DESC:Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress Gene:SPB4(YFL002C)|FD-Score:3.23|P-value:6.09E-4|Clearance:0.03||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TFB3(YDR460W)|FD-Score:6|P-value:9.85E-10|Clearance:0.45||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:YGR115C(YGR115C_d)|FD-Score:4.17|P-value:1.55E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YJR012C(YJR012C_p)|FD-Score:3.21|P-value:6.65E-4|Clearance:0||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W Gene:YJU2(YKL095W)|FD-Score:-3.52|P-value:2.18E-4|Clearance:0||SGD DESC:Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing Gene:ALA1(YOR335C)|FD-Score:3.97|P-value:3.61E-5|Clearance:0.14||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:ALG2(YGL065C)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.07||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:BET2(YPR176C)|FD-Score:5.76|P-value:4.13E-9|Clearance:0.45||SGD DESC:Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:BET4(YJL031C)|FD-Score:5.48|P-value:2.18E-8|Clearance:0.45||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:BRR6(YGL247W)|FD-Score:5.78|P-value:3.84E-9|Clearance:0.45||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:COG2(YGR120C)|FD-Score:3.82|P-value:6.62E-5|Clearance:0.08||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:FAL1(YDR021W)|FD-Score:4.39|P-value:5.63E-6|Clearance:0.13||SGD DESC:Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases Gene:FRQ1(YDR373W)|FD-Score:3.24|P-value:5.92E-4|Clearance:0.01||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:GFA1(YKL104C)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication Gene:GLC7(YER133W)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.08||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GLE1(YDL207W)|FD-Score:3.65|P-value:1.33E-4|Clearance:0.13||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:LSM2(YBL026W)|FD-Score:5.33|P-value:4.87E-8|Clearance:0.45||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MAS1(YLR163C)|FD-Score:3.74|P-value:9.15E-5|Clearance:0.09||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MCD4(YKL165C)|FD-Score:4.26|P-value:1.03E-5|Clearance:0.09||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MYO2(YOR326W)|FD-Score:5.99|P-value:1.05E-9|Clearance:0.45||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication Gene:NOB1(YOR056C)|FD-Score:5.41|P-value:3.19E-8|Clearance:0.45||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:NOP56(YLR197W)|FD-Score:4.88|P-value:5.29E-7|Clearance:0.23||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NSL1(YPL233W)|FD-Score:-3.37|P-value:3.72E-4|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NUF2(YOL069W)|FD-Score:4.65|P-value:1.65E-6|Clearance:0.26||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:NUG1(YER006W)|FD-Score:-4.64|P-value:1.75E-6|Clearance:0||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:RER2(YBR002C)|FD-Score:3.35|P-value:4.08E-4|Clearance:0.1||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RHO1(YPR165W)|FD-Score:3.52|P-value:2.15E-4|Clearance:0.04||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPL15A(YLR029C)|FD-Score:-3.73|P-value:9.53E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPN3(YER021W)|FD-Score:4.65|P-value:1.63E-6|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN6(YDL097C)|FD-Score:4.13|P-value:1.79E-5|Clearance:0.16||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RSC58(YLR033W)|FD-Score:3.83|P-value:6.49E-5|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:3.21|P-value:6.68E-4|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC6(YIL068C)|FD-Score:-5.26|P-value:7.38E-8|Clearance:0||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SEN1(YLR430W)|FD-Score:3.48|P-value:2.49E-4|Clearance:0.02||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SFH1(YLR321C)|FD-Score:3.39|P-value:3.55E-4|Clearance:0.04||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SLD2(YKL108W)|FD-Score:3.11|P-value:9.49E-4|Clearance:0.09||SGD DESC:Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress Gene:SPB4(YFL002C)|FD-Score:3.23|P-value:6.09E-4|Clearance:0.03||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TFB3(YDR460W)|FD-Score:6|P-value:9.85E-10|Clearance:0.45||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:YGR115C(YGR115C_d)|FD-Score:4.17|P-value:1.55E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YJR012C(YJR012C_p)|FD-Score:3.21|P-value:6.65E-4|Clearance:0||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W Gene:YJU2(YKL095W)|FD-Score:-3.52|P-value:2.18E-4|Clearance:0||SGD DESC:Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 870305
Download HOP data (tab-delimited text)  (excel)
Gene:ADA2(YDR448W)|FD-Score:-4.27|P-value:9.95E-6||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ARG82(YDR173C)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARL1(YBR164C)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARR3(YPR201W)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:BAS1(YKR099W)|FD-Score:4.79|P-value:8.41E-7||SGD DESC:Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes Gene:BOP2(YLR267W_p)|FD-Score:-3.85|P-value:5.84E-5||SGD DESC:Protein of unknown function Gene:BRE5(YNR051C)|FD-Score:3.82|P-value:6.55E-5||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CAT8(YMR280C)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CCM1(YGR150C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CCW12(YLR110C)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CNN1(YFR046C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T Gene:COG7(YGL005C)|FD-Score:6|P-value:9.81E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPR2(YHR057C)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CRT10(YOL063C)|FD-Score:-4.16|P-value:1.57E-5||SGD DESC:Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat Gene:CWC27(YPL064C)|FD-Score:-6.36|P-value:9.90E-11||SGD DESC:Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress Gene:ECM19(YLR390W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:EMI5(YOL071W)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Subunit of succinate dehydrogenase, which couples succinate oxidation to ubiquinone reduction; required for FAD cofactor attachment to Sdh1p; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma) Gene:ENB1(YOL158C)|FD-Score:3.9|P-value:4.84E-5||SGD DESC:Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment Gene:ERG4(YGL012W)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ESC2(YDR363W)|FD-Score:4.61|P-value:1.97E-6||SGD DESC:Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member Gene:FAR7(YFR008W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FKH2(YNL068C)|FD-Score:5.66|P-value:7.72E-9||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FKS3(YMR306W)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FLC2(YAL053W)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FRT2(YAL028W)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:GAL4(YPL248C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p Gene:GDB1(YPR184W)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GEP3(YOR205C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GLN3(YER040W)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GSP2(YOR185C)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HAL1(YPR005C)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:HAP4(YKL109W)|FD-Score:-3.86|P-value:5.78E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HAT1(YPL001W)|FD-Score:4.47|P-value:3.83E-6||SGD DESC:Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair Gene:HER2(YMR293C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HIR3(YJR140C)|FD-Score:5.78|P-value:3.80E-9||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:HPF1(YOL155C)|FD-Score:6.9|P-value:2.54E-12||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:HXT8(YJL214W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IGD1(YFR017C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication Gene:INM2(YDR287W)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:IRC18(YJL037W)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:KCH1(YJR054W_p)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate Gene:LSB3(YFR024C-A)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MCH5(YOR306C)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MET14(YKL001C)|FD-Score:6.43|P-value:6.54E-11||SGD DESC:Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Gene:MGA1(YGR249W)|FD-Score:-3.33|P-value:4.30E-4||SGD DESC:Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants Gene:MGM101(YJR144W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MID2(YLR332W)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MRPL37(YBR268W)|FD-Score:3.73|P-value:9.76E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH2(YOL090W)|FD-Score:5.66|P-value:7.53E-9||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MST1(YKL194C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NCR1(YPL006W)|FD-Score:-4.65|P-value:1.63E-6||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:NNF2(YGR089W)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:OGG1(YML060W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:PAC10(YGR078C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAM17(YKR065C)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress Gene:PBP4(YDL053C)|FD-Score:4.39|P-value:5.78E-6||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PCL1(YNL289W)|FD-Score:4.36|P-value:6.53E-6||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PDB1(YBR221C)|FD-Score:-5.06|P-value:2.06E-7||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PDH1(YPR002W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PET112(YBL080C)|FD-Score:5.6|P-value:1.08E-8||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PEX27(YOR193W)|FD-Score:5|P-value:2.84E-7||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PFA3(YNL326C)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions Gene:PHO87(YCR037C)|FD-Score:-8.26|P-value:7.28E-17||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PML39(YML107C)|FD-Score:-4.95|P-value:3.62E-7||SGD DESC:Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes Gene:PRO2(YOR323C)|FD-Score:-6.6|P-value:2.07E-11||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PSO2(YMR137C)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress Gene:PUF4(YGL014W)|FD-Score:4.38|P-value:5.98E-6||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:PUT1(YLR142W)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source Gene:RAD6(YGL058W)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p Gene:RAV1(YJR033C)|FD-Score:7.39|P-value:7.35E-14||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:REI1(YBR267W)|FD-Score:5.78|P-value:3.64E-9||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RGA2(YDR379W)|FD-Score:-3.91|P-value:4.65E-5||SGD DESC:GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p Gene:RGP1(YDR137W)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RML2(YEL050C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL13A(YDL082W)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:SKI2(YLR398C)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SPC2(YML055W)|FD-Score:-4.27|P-value:9.76E-6||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:SPS18(YNL204C)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SUL1(YBR294W)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SWA2(YDR320C)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TBS1(YBR150C)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication Gene:TDA11(YHR159W_p)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:THR1(YHR025W)|FD-Score:4.47|P-value:3.91E-6||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TUF1(YOR187W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBX2(YML013W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UBX5(YDR330W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p Gene:UGA2(YBR006W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:URA1(YKL216W)|FD-Score:-3.97|P-value:3.53E-5||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VAC7(YNL054W)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VFA1(YER128W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VPS13(YLL040C)|FD-Score:-3.39|P-value:3.43E-4||SGD DESC:Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively Gene:VPS51(YKR020W)|FD-Score:3.46|P-value:2.65E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:WTM2(YOR229W)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YAP1802(YGR241C)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YBL062W(YBL062W_d)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL065W(YBL065W_d)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene Gene:YBR296C-A(YBR296C-A_p)|FD-Score:6.11|P-value:4.98E-10||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBT1(YLL048C)|FD-Score:3.91|P-value:4.63E-5||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YCL012C(YCL012C)|FD-Score:4.43|P-value:4.75E-6||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YDL129W(YDL129W_p)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR102C(YDR102C_d)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index Gene:YDR115W(YDR115W_p)|FD-Score:4.27|P-value:9.83E-6||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YEL008W(YEL008W_d)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Hypothetical protein predicted to be involved in metabolism Gene:YFL019C(YFL019C_d)|FD-Score:5.42|P-value:2.93E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YFL052W(YFL052W_p)|FD-Score:5.05|P-value:2.25E-7||SGD DESC:Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity Gene:YGR139W(YGR139W_d)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL060W(YIL060W_p)|FD-Score:3.83|P-value:6.32E-5||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIL089W(YIL089W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking Gene:YIL165C(YIL165C_p)|FD-Score:3.76|P-value:8.36E-5||SGD DESC:Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene Gene:YLR040C(YLR040C_p)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YMR160W(YMR160W_p)|FD-Score:6.51|P-value:3.79E-11||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YMR172C-A(YMR172C-A_d)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR181C(YMR181C_p)|FD-Score:-4.16|P-value:1.61E-5||SGD DESC:Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication Gene:YNL162W-A(YNL162W-A_p)|FD-Score:4.53|P-value:2.90E-6||SGD DESC:Putative protein of unknown function; identified by homology Gene:YNL217W(YNL217W_p)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate Gene:YNL319W(YNL319W_d)|FD-Score:7.02|P-value:1.11E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YPL152W-A(YPL152W-A_p)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPR114W(YPR114W_p)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Putative protein of unknown function Gene:YPT31(YER031C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ZDS1(YMR273C)|FD-Score:3.74|P-value:9.14E-5||SGD DESC:Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication Gene:ADA2(YDR448W)|FD-Score:-4.27|P-value:9.95E-6||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ARG82(YDR173C)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARL1(YBR164C)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARR3(YPR201W)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:BAS1(YKR099W)|FD-Score:4.79|P-value:8.41E-7||SGD DESC:Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes Gene:BOP2(YLR267W_p)|FD-Score:-3.85|P-value:5.84E-5||SGD DESC:Protein of unknown function Gene:BRE5(YNR051C)|FD-Score:3.82|P-value:6.55E-5||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CAT8(YMR280C)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CCM1(YGR150C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CCW12(YLR110C)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CNN1(YFR046C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T Gene:COG7(YGL005C)|FD-Score:6|P-value:9.81E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPR2(YHR057C)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CRT10(YOL063C)|FD-Score:-4.16|P-value:1.57E-5||SGD DESC:Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat Gene:CWC27(YPL064C)|FD-Score:-6.36|P-value:9.90E-11||SGD DESC:Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress Gene:ECM19(YLR390W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:EMI5(YOL071W)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Subunit of succinate dehydrogenase, which couples succinate oxidation to ubiquinone reduction; required for FAD cofactor attachment to Sdh1p; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma) Gene:ENB1(YOL158C)|FD-Score:3.9|P-value:4.84E-5||SGD DESC:Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment Gene:ERG4(YGL012W)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ESC2(YDR363W)|FD-Score:4.61|P-value:1.97E-6||SGD DESC:Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member Gene:FAR7(YFR008W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FKH2(YNL068C)|FD-Score:5.66|P-value:7.72E-9||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FKS3(YMR306W)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FLC2(YAL053W)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FRT2(YAL028W)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:GAL4(YPL248C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p Gene:GDB1(YPR184W)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GEP3(YOR205C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GLN3(YER040W)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GSP2(YOR185C)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HAL1(YPR005C)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:HAP4(YKL109W)|FD-Score:-3.86|P-value:5.78E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HAT1(YPL001W)|FD-Score:4.47|P-value:3.83E-6||SGD DESC:Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair Gene:HER2(YMR293C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HIR3(YJR140C)|FD-Score:5.78|P-value:3.80E-9||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:HPF1(YOL155C)|FD-Score:6.9|P-value:2.54E-12||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:HXT8(YJL214W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IGD1(YFR017C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication Gene:INM2(YDR287W)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:IRC18(YJL037W)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:KCH1(YJR054W_p)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate Gene:LSB3(YFR024C-A)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MCH5(YOR306C)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MET14(YKL001C)|FD-Score:6.43|P-value:6.54E-11||SGD DESC:Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Gene:MGA1(YGR249W)|FD-Score:-3.33|P-value:4.30E-4||SGD DESC:Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants Gene:MGM101(YJR144W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MID2(YLR332W)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MRPL37(YBR268W)|FD-Score:3.73|P-value:9.76E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH2(YOL090W)|FD-Score:5.66|P-value:7.53E-9||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MST1(YKL194C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NCR1(YPL006W)|FD-Score:-4.65|P-value:1.63E-6||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:NNF2(YGR089W)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:OGG1(YML060W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:PAC10(YGR078C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAM17(YKR065C)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress Gene:PBP4(YDL053C)|FD-Score:4.39|P-value:5.78E-6||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PCL1(YNL289W)|FD-Score:4.36|P-value:6.53E-6||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PDB1(YBR221C)|FD-Score:-5.06|P-value:2.06E-7||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PDH1(YPR002W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PET112(YBL080C)|FD-Score:5.6|P-value:1.08E-8||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PEX27(YOR193W)|FD-Score:5|P-value:2.84E-7||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PFA3(YNL326C)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions Gene:PHO87(YCR037C)|FD-Score:-8.26|P-value:7.28E-17||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PML39(YML107C)|FD-Score:-4.95|P-value:3.62E-7||SGD DESC:Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes Gene:PRO2(YOR323C)|FD-Score:-6.6|P-value:2.07E-11||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PSO2(YMR137C)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress Gene:PUF4(YGL014W)|FD-Score:4.38|P-value:5.98E-6||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:PUT1(YLR142W)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source Gene:RAD6(YGL058W)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p Gene:RAV1(YJR033C)|FD-Score:7.39|P-value:7.35E-14||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:REI1(YBR267W)|FD-Score:5.78|P-value:3.64E-9||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RGA2(YDR379W)|FD-Score:-3.91|P-value:4.65E-5||SGD DESC:GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p Gene:RGP1(YDR137W)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RML2(YEL050C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL13A(YDL082W)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:SKI2(YLR398C)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SPC2(YML055W)|FD-Score:-4.27|P-value:9.76E-6||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:SPS18(YNL204C)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SUL1(YBR294W)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SWA2(YDR320C)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TBS1(YBR150C)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication Gene:TDA11(YHR159W_p)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:THR1(YHR025W)|FD-Score:4.47|P-value:3.91E-6||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TUF1(YOR187W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBX2(YML013W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UBX5(YDR330W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p Gene:UGA2(YBR006W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:URA1(YKL216W)|FD-Score:-3.97|P-value:3.53E-5||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VAC7(YNL054W)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VFA1(YER128W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VPS13(YLL040C)|FD-Score:-3.39|P-value:3.43E-4||SGD DESC:Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively Gene:VPS51(YKR020W)|FD-Score:3.46|P-value:2.65E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:WTM2(YOR229W)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YAP1802(YGR241C)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YBL062W(YBL062W_d)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL065W(YBL065W_d)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene Gene:YBR296C-A(YBR296C-A_p)|FD-Score:6.11|P-value:4.98E-10||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBT1(YLL048C)|FD-Score:3.91|P-value:4.63E-5||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YCL012C(YCL012C)|FD-Score:4.43|P-value:4.75E-6||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YDL129W(YDL129W_p)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR102C(YDR102C_d)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index Gene:YDR115W(YDR115W_p)|FD-Score:4.27|P-value:9.83E-6||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YEL008W(YEL008W_d)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Hypothetical protein predicted to be involved in metabolism Gene:YFL019C(YFL019C_d)|FD-Score:5.42|P-value:2.93E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YFL052W(YFL052W_p)|FD-Score:5.05|P-value:2.25E-7||SGD DESC:Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity Gene:YGR139W(YGR139W_d)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL060W(YIL060W_p)|FD-Score:3.83|P-value:6.32E-5||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIL089W(YIL089W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking Gene:YIL165C(YIL165C_p)|FD-Score:3.76|P-value:8.36E-5||SGD DESC:Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene Gene:YLR040C(YLR040C_p)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YMR160W(YMR160W_p)|FD-Score:6.51|P-value:3.79E-11||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YMR172C-A(YMR172C-A_d)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR181C(YMR181C_p)|FD-Score:-4.16|P-value:1.61E-5||SGD DESC:Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication Gene:YNL162W-A(YNL162W-A_p)|FD-Score:4.53|P-value:2.90E-6||SGD DESC:Putative protein of unknown function; identified by homology Gene:YNL217W(YNL217W_p)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate Gene:YNL319W(YNL319W_d)|FD-Score:7.02|P-value:1.11E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YPL152W-A(YPL152W-A_p)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPR114W(YPR114W_p)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Putative protein of unknown function Gene:YPT31(YER031C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ZDS1(YMR273C)|FD-Score:3.74|P-value:9.14E-5||SGD DESC:Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR460W6.009.85E-100.45TFB3Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit
YOR326W5.991.05E-90.45MYO2Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication
YGL247W5.783.84E-90.45BRR6Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
YPR176C5.764.13E-90.45BET2Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YJL031C5.482.18E-80.45BET4Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YOR056C5.413.19E-80.45NOB1Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress
YBL026W5.334.87E-80.45LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YLR197W4.885.29E-70.23NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YER021W4.651.63E-60.00RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YOL069W4.651.65E-60.26NUF2Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YDR021W4.395.63E-60.13FAL1Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases
YKL165C4.261.03E-50.09MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YGR115C_d4.171.55E-50.03YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YDL097C4.131.79E-50.16RPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
YOR335C3.973.61E-50.14ALA1Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJR033C7.397.35E-14RAV1Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
YNL319W_d7.021.11E-12YNL319W_dDubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14
YOL155C6.902.54E-12HPF1Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines
YMR160W_p6.513.79E-11YMR160W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress
YKL001C6.436.54E-11MET14Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism
YBR296C-A_p6.114.98E-10YBR296C-A_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YGL005C6.009.81E-10COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YBR267W5.783.64E-9REI1Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
YJR140C5.783.80E-9HIR3Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein
YOL090W5.667.53E-9MSH2Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP
YNL068C5.667.72E-9FKH2Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication
YBL080C5.601.08E-8PET112Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog
YFL019C_d5.422.93E-8YFL019C_dDubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene
YOR306C5.181.09E-7MCH5Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport
YFL052W_p5.052.25E-7YFL052W_pPutative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity

GO enrichment analysis for SGTC_1727
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1201.69E-20SGTC_20415107442 45.3 μMChembridge (Fragment library)7930050.132075RSC complex & mRNA processing
0.0952.07E-13SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.0625Golgi
0.0939.09E-13SGTC_624k048-0052 28.0 μMChemDiv (Drug-like library)68355310.0704225Golgi
0.0921.08E-12SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.138462RSC & ERG11
0.0872.38E-11SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.0350877Golgi
0.0872.51E-11SGTC_21095483026 200.0 μMChembridge (Fragment library)7593350.111111RSC complex & mRNA processing
0.0863.91E-11SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.047619Golgi
0.0855.86E-11SGTC_590k048-0076 22.4 μMChemDiv (Drug-like library)68328140.0757576RSC & ERG11
0.0858.14E-11SGTC_21876125751 200.0 μMChembridge (Fragment library)8744980.0793651RSC complex & mRNA processing
0.0849.01E-11SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.125
0.0831.33E-10SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.0649351Golgi
0.0822.51E-10SGTC_33259138760 55.2 μMChembridge (Drug-like library)438449590.0909091Golgi
0.0773.20E-9SGTC_220964-0034 14.5 μMChemDiv (Drug-like library)1384020.1unfolded protein response
0.0765.58E-9SGTC_560957-0365 83.2 μMChemDiv (Drug-like library)67523600.115385Golgi
0.0731.71E-8SGTC_32779138724 49.5 μMChembridge (Drug-like library)159434180.0655738Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1735st03431056.75 μM0.789474871153TimTec (Natural product derivative library)293.530483.13403Golgi
SGTC_1732st03430851.82 μM0.666667830119TimTec (Natural product derivative library)277.531083.63602
SGTC_1723st0343072.55 μM0.641026867466TimTec (Natural product derivative library)297.949563.81402mitochondrial processes
SGTC_1866st05763864 μM0.568182688802TimTec (Natural product derivative library)312.316643.08705
SGTC_1722st0343043.65 μM0.5641034036148TimTec (Natural product derivative library)263.50453.1502mitochondrial processes
SGTC_1111tnp00062510.7 nM0.543478676306TimTec (Natural product library)268.264082.87814
SGTC_1833st05598471 μM0.543478676288TimTec (Natural product derivative library)282.290663.10304
SGTC_15412',3',6-trimethoxyflavone64 μM0.510204688801TimTec (Pure natural product library)312.316643.08705
SGTC_26222-methoxyxanthone59.94 μM0.48837271034Microsource (Natural product library)226.22742.94803
SGTC_1860st05622874.6 μM0.479167688679TimTec (Natural product derivative library)268.264082.87814