st035615

(1R,3R)-5-bromo-1,3-bis(2-methylprop-2-enyl)-1,2,3,4-tetrahydroisoquinoline hydrochloride

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1729
Screen concentration 46.0 μM
Source TimTec (Natural product derivative library)
PubChem CID 17385451
SMILES CC(=C)CC1CC2=C(C=CC=C2Br)C(N1)CC(=C)C.Cl
Standardized SMILES CC(=C)CC1Cc2c(Br)cccc2C(CC(=C)C)N1
Molecular weight 356.7282
ALogP 5.4
H-bond donor count 2
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 21.88
% growth inhibition (Hom. pool) 7.08


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17385451
Download HIP data (tab-delimited text)  (excel)
Gene:BET4(YJL031C)|FD-Score:4.23|P-value:1.15E-5|Clearance:0.24||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:BET5(YML077W)|FD-Score:-6.33|P-value:1.20E-10|Clearance:0||SGD DESC:Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi Gene:CDC28(YBR160W)|FD-Score:-5.34|P-value:4.68E-8|Clearance:0||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:CDC53(YDL132W)|FD-Score:4.25|P-value:1.06E-5|Clearance:0.01||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:DBP2(YNL112W)|FD-Score:3.99|P-value:3.26E-5|Clearance:0.49||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:GLE1(YDL207W)|FD-Score:5.34|P-value:4.74E-8|Clearance:0.82||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:ILS1(YBL076C)|FD-Score:3.5|P-value:2.29E-4|Clearance:0.11||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:MIA40(YKL195W)|FD-Score:3.16|P-value:8.00E-4|Clearance:0.01||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NUP57(YGR119C)|FD-Score:3.38|P-value:3.61E-4|Clearance:0.23||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:ORC5(YNL261W)|FD-Score:4.24|P-value:1.13E-5|Clearance:0.01||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PAN1(YIR006C)|FD-Score:3.14|P-value:8.32E-4|Clearance:0.01||SGD DESC:Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease Gene:PIS1(YPR113W)|FD-Score:3.13|P-value:8.59E-4|Clearance:0.05||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:PRE1(YER012W)|FD-Score:-3.43|P-value:3.02E-4|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRE2(YPR103W)|FD-Score:-5|P-value:2.93E-7|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RPN8(YOR261C)|FD-Score:5.07|P-value:1.97E-7|Clearance:0.82||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RSC6(YCR052W)|FD-Score:3.15|P-value:8.27E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:STH1(YIL126W)|FD-Score:5.65|P-value:8.24E-9|Clearance:0.82||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SWD2(YKL018W)|FD-Score:-3.25|P-value:5.85E-4|Clearance:0||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TAF12(YDR145W)|FD-Score:5.6|P-value:1.08E-8|Clearance:0.82||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TAF3(YPL011C)|FD-Score:-3.16|P-value:7.96E-4|Clearance:0||SGD DESC:TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation Gene:TAF5(YBR198C)|FD-Score:5.56|P-value:1.37E-8|Clearance:0.82||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TOM40(YMR203W)|FD-Score:3.4|P-value:3.42E-4|Clearance:0.02||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:WRS1(YOL097C)|FD-Score:-7.03|P-value:1.03E-12|Clearance:0||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:BET4(YJL031C)|FD-Score:4.23|P-value:1.15E-5|Clearance:0.24||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:BET5(YML077W)|FD-Score:-6.33|P-value:1.20E-10|Clearance:0||SGD DESC:Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi Gene:CDC28(YBR160W)|FD-Score:-5.34|P-value:4.68E-8|Clearance:0||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:CDC53(YDL132W)|FD-Score:4.25|P-value:1.06E-5|Clearance:0.01||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:DBP2(YNL112W)|FD-Score:3.99|P-value:3.26E-5|Clearance:0.49||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:GLE1(YDL207W)|FD-Score:5.34|P-value:4.74E-8|Clearance:0.82||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:ILS1(YBL076C)|FD-Score:3.5|P-value:2.29E-4|Clearance:0.11||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:MIA40(YKL195W)|FD-Score:3.16|P-value:8.00E-4|Clearance:0.01||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NUP57(YGR119C)|FD-Score:3.38|P-value:3.61E-4|Clearance:0.23||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:ORC5(YNL261W)|FD-Score:4.24|P-value:1.13E-5|Clearance:0.01||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PAN1(YIR006C)|FD-Score:3.14|P-value:8.32E-4|Clearance:0.01||SGD DESC:Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease Gene:PIS1(YPR113W)|FD-Score:3.13|P-value:8.59E-4|Clearance:0.05||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:PRE1(YER012W)|FD-Score:-3.43|P-value:3.02E-4|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRE2(YPR103W)|FD-Score:-5|P-value:2.93E-7|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RPN8(YOR261C)|FD-Score:5.07|P-value:1.97E-7|Clearance:0.82||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RSC6(YCR052W)|FD-Score:3.15|P-value:8.27E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:STH1(YIL126W)|FD-Score:5.65|P-value:8.24E-9|Clearance:0.82||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SWD2(YKL018W)|FD-Score:-3.25|P-value:5.85E-4|Clearance:0||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TAF12(YDR145W)|FD-Score:5.6|P-value:1.08E-8|Clearance:0.82||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TAF3(YPL011C)|FD-Score:-3.16|P-value:7.96E-4|Clearance:0||SGD DESC:TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation Gene:TAF5(YBR198C)|FD-Score:5.56|P-value:1.37E-8|Clearance:0.82||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TOM40(YMR203W)|FD-Score:3.4|P-value:3.42E-4|Clearance:0.02||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:WRS1(YOL097C)|FD-Score:-7.03|P-value:1.03E-12|Clearance:0||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17385451
Download HOP data (tab-delimited text)  (excel)
Gene:ALG8(YOR067C)|FD-Score:5.22|P-value:8.80E-8||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:APJ1(YNL077W)|FD-Score:4.18|P-value:1.49E-5||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARN2(YHL047C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:BBC1(YJL020C)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches Gene:BFA1(YJR053W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis Gene:CAP1(YKL007W)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CHS7(YHR142W)|FD-Score:5.34|P-value:4.65E-8||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:DAL3(YIR032C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DIC1(YLR348C)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:DNL4(YOR005C)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth Gene:ELC1(YPL046C)|FD-Score:-4.92|P-value:4.25E-7||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:ENV7(YPL236C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:ERD1(YDR414C)|FD-Score:5.68|P-value:6.87E-9||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:FAA2(YER015W)|FD-Score:6.39|P-value:8.06E-11||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FAR11(YNL127W)|FD-Score:4.27|P-value:9.59E-6||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FKS3(YMR306W)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FLO1(YAR050W)|FD-Score:4.33|P-value:7.33E-6||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:FRT2(YAL028W)|FD-Score:-4.71|P-value:1.22E-6||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:GCS1(YDL226C)|FD-Score:4.94|P-value:3.92E-7||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDB1(YPR184W)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GIP3(YPL137C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:IFA38(YBR159W)|FD-Score:-5.24|P-value:8.06E-8||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IML2(YJL082W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:LAA1(YJL207C)|FD-Score:-5.27|P-value:6.92E-8||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LYS9(YNR050C)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 Gene:MDJ2(YNL328C)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MEC3(YLR288C)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MET22(YOL064C)|FD-Score:5.29|P-value:5.96E-8||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MGM101(YJR144W)|FD-Score:5.09|P-value:1.83E-7||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MSN5(YDR335W)|FD-Score:4.31|P-value:8.10E-6||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MTD1(YKR080W)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NKP2(YLR315W)|FD-Score:-3.11|P-value:9.42E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:NOP13(YNL175C)|FD-Score:7.41|P-value:6.30E-14||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NSR1(YGR159C)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:OCA6(YDR067C_p)|FD-Score:3.81|P-value:6.94E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PEX4(YGR133W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis Gene:PHO87(YCR037C)|FD-Score:5.36|P-value:4.19E-8||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PIG1(YLR273C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase Gene:PKH3(YDR466W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PKR1(YMR123W)|FD-Score:10|P-value:6.19E-24||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PML1(YLR016C)|FD-Score:-3.29|P-value:4.92E-4||SGD DESC:Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p Gene:PRO2(YOR323C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTK2(YJR059W)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUB1(YNL016W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:RAV1(YJR033C)|FD-Score:7.9|P-value:1.41E-15||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RFU1(YLR073C)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RGM1(YMR182C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y-prime telomeric elements and subtelomeric COS genes; RGM1 has a paralog, USV1, that arose from the whole genome duplication Gene:RPL34A(YER056C-A)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPL38(YLR325C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RPL40A(YIL148W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RPN4(YDL020C)|FD-Score:-5.05|P-value:2.19E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS17A(YML024W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RPS1A(YLR441C)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:4.71|P-value:1.27E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RSA1(YPL193W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:SAC1(YKL212W)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC7(YDR389W)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SEC66(YBR171W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIZ1(YDR409W)|FD-Score:11.2|P-value:3.38E-29||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SMI1(YGR229C)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SOL1(YNR034W)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPE3(YPR069C)|FD-Score:-3.1|P-value:9.79E-4||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SPO11(YHL022C)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SSN8(YNL025C)|FD-Score:4.93|P-value:4.12E-7||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:TGL1(YKL140W)|FD-Score:7.03|P-value:1.02E-12||SGD DESC:Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes Gene:TOK1(YJL093C)|FD-Score:4.31|P-value:8.22E-6||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:TRM1(YDR120C)|FD-Score:-4.54|P-value:2.88E-6||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:USA1(YML029W)|FD-Score:-3.92|P-value:4.41E-5||SGD DESC:Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p Gene:VMA9(YCL005W-A)|FD-Score:4.39|P-value:5.55E-6||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPH2(YKL119C)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS25(YJR102C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:4.29|P-value:8.78E-6||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS52(YDR484W)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS65(YLR322W_d)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YBR027C(YBR027C_d)|FD-Score:4.3|P-value:8.67E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR184W(YBR184W_p)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YBR238C(YBR238C)|FD-Score:6.45|P-value:5.56E-11||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR242W(YBR242W_p)|FD-Score:-3.23|P-value:6.08E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YCL075W(YCL075W)|FD-Score:4.48|P-value:3.67E-6||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YEL1(YBL060W)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YER187W(YER187W_p)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Putative protein of unknown function; induced in respiratory-deficient cells Gene:YGR107W(YGR107W_d)|FD-Score:3.83|P-value:6.45E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR122W(YGR122W)|FD-Score:7.95|P-value:9.55E-16||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YJL211C(YJL211C_d)|FD-Score:5.14|P-value:1.37E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YKL044W(YKL044W_p)|FD-Score:-4.4|P-value:5.41E-6||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL077W(YKL077W_p)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKL115C(YKL115C_d)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YLL056C(YLL056C_p)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin Gene:YLR236C(YLR236C_d)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR311C(YLR311C_d)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR155W(YMR155W_p)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YMR172C-A(YMR172C-A_d)|FD-Score:-4.78|P-value:8.90E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR295C(YMR295C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; YMR295C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL144C(YNL144C_p)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:YNL179C(YNL179C_d)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance Gene:YOL097W-A(YOL097W-A_p)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPL182C(YPL182C_d)|FD-Score:5.83|P-value:2.72E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPT6(YLR262C)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YPT7(YML001W)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 Gene:ALG8(YOR067C)|FD-Score:5.22|P-value:8.80E-8||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:APJ1(YNL077W)|FD-Score:4.18|P-value:1.49E-5||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARN2(YHL047C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:BBC1(YJL020C)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches Gene:BFA1(YJR053W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis Gene:CAP1(YKL007W)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CHS7(YHR142W)|FD-Score:5.34|P-value:4.65E-8||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:DAL3(YIR032C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DIC1(YLR348C)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:DNL4(YOR005C)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth Gene:ELC1(YPL046C)|FD-Score:-4.92|P-value:4.25E-7||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:ENV7(YPL236C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:ERD1(YDR414C)|FD-Score:5.68|P-value:6.87E-9||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:FAA2(YER015W)|FD-Score:6.39|P-value:8.06E-11||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FAR11(YNL127W)|FD-Score:4.27|P-value:9.59E-6||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FKS3(YMR306W)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FLO1(YAR050W)|FD-Score:4.33|P-value:7.33E-6||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:FRT2(YAL028W)|FD-Score:-4.71|P-value:1.22E-6||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:GCS1(YDL226C)|FD-Score:4.94|P-value:3.92E-7||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDB1(YPR184W)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GIP3(YPL137C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:IFA38(YBR159W)|FD-Score:-5.24|P-value:8.06E-8||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IML2(YJL082W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:LAA1(YJL207C)|FD-Score:-5.27|P-value:6.92E-8||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LYS9(YNR050C)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 Gene:MDJ2(YNL328C)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MEC3(YLR288C)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MET22(YOL064C)|FD-Score:5.29|P-value:5.96E-8||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MGM101(YJR144W)|FD-Score:5.09|P-value:1.83E-7||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MSN5(YDR335W)|FD-Score:4.31|P-value:8.10E-6||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MTD1(YKR080W)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NKP2(YLR315W)|FD-Score:-3.11|P-value:9.42E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:NOP13(YNL175C)|FD-Score:7.41|P-value:6.30E-14||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NSR1(YGR159C)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:OCA6(YDR067C_p)|FD-Score:3.81|P-value:6.94E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PEX4(YGR133W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis Gene:PHO87(YCR037C)|FD-Score:5.36|P-value:4.19E-8||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PIG1(YLR273C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase Gene:PKH3(YDR466W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PKR1(YMR123W)|FD-Score:10|P-value:6.19E-24||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PML1(YLR016C)|FD-Score:-3.29|P-value:4.92E-4||SGD DESC:Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p Gene:PRO2(YOR323C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTK2(YJR059W)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUB1(YNL016W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:RAV1(YJR033C)|FD-Score:7.9|P-value:1.41E-15||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RFU1(YLR073C)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RGM1(YMR182C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y-prime telomeric elements and subtelomeric COS genes; RGM1 has a paralog, USV1, that arose from the whole genome duplication Gene:RPL34A(YER056C-A)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPL38(YLR325C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RPL40A(YIL148W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RPN4(YDL020C)|FD-Score:-5.05|P-value:2.19E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS17A(YML024W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RPS1A(YLR441C)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:4.71|P-value:1.27E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RSA1(YPL193W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:SAC1(YKL212W)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC7(YDR389W)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SEC66(YBR171W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIZ1(YDR409W)|FD-Score:11.2|P-value:3.38E-29||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SMI1(YGR229C)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SOL1(YNR034W)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPE3(YPR069C)|FD-Score:-3.1|P-value:9.79E-4||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SPO11(YHL022C)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SSN8(YNL025C)|FD-Score:4.93|P-value:4.12E-7||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:TGL1(YKL140W)|FD-Score:7.03|P-value:1.02E-12||SGD DESC:Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes Gene:TOK1(YJL093C)|FD-Score:4.31|P-value:8.22E-6||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:TRM1(YDR120C)|FD-Score:-4.54|P-value:2.88E-6||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:USA1(YML029W)|FD-Score:-3.92|P-value:4.41E-5||SGD DESC:Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p Gene:VMA9(YCL005W-A)|FD-Score:4.39|P-value:5.55E-6||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPH2(YKL119C)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS25(YJR102C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:4.29|P-value:8.78E-6||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS52(YDR484W)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS65(YLR322W_d)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YBR027C(YBR027C_d)|FD-Score:4.3|P-value:8.67E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR184W(YBR184W_p)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YBR238C(YBR238C)|FD-Score:6.45|P-value:5.56E-11||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR242W(YBR242W_p)|FD-Score:-3.23|P-value:6.08E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YCL075W(YCL075W)|FD-Score:4.48|P-value:3.67E-6||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YEL1(YBL060W)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YER187W(YER187W_p)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Putative protein of unknown function; induced in respiratory-deficient cells Gene:YGR107W(YGR107W_d)|FD-Score:3.83|P-value:6.45E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR122W(YGR122W)|FD-Score:7.95|P-value:9.55E-16||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YJL211C(YJL211C_d)|FD-Score:5.14|P-value:1.37E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YKL044W(YKL044W_p)|FD-Score:-4.4|P-value:5.41E-6||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL077W(YKL077W_p)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKL115C(YKL115C_d)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YLL056C(YLL056C_p)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin Gene:YLR236C(YLR236C_d)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR311C(YLR311C_d)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR155W(YMR155W_p)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YMR172C-A(YMR172C-A_d)|FD-Score:-4.78|P-value:8.90E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR295C(YMR295C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; YMR295C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL144C(YNL144C_p)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:YNL179C(YNL179C_d)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance Gene:YOL097W-A(YOL097W-A_p)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPL182C(YPL182C_d)|FD-Score:5.83|P-value:2.72E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPT6(YLR262C)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YPT7(YML001W)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL126W5.658.24E-90.82STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YDR145W5.601.08E-80.82TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YBR198C5.561.37E-80.82TAF5Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YDL207W5.344.74E-80.82GLE1Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export
YOR261C5.071.97E-70.82RPN8Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p
YDL132W4.251.06E-50.01CDC53Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation
YNL261W4.241.13E-50.01ORC5Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YJL031C4.231.15E-50.24BET4Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YNL112W3.993.26E-50.49DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YBL076C3.502.29E-40.11ILS1Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A
YMR203W3.403.42E-40.02TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YGR119C3.383.61E-40.23NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YKL195W3.168.00E-40.01MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YCR052W3.158.27E-40.00RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YIR006C3.148.32E-40.01PAN1Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR409W11.203.38E-29SIZ1SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring
YMR123W10.006.19E-24PKR1V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
YGR122W7.959.55E-16YGR122WProtein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
YJR033C7.901.41E-15RAV1Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
YNL175C7.416.30E-14NOP13Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress
YKL140W7.031.02E-12TGL1Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
YBR238C6.455.56E-11YBR238CMitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span
YER015W6.398.06E-11FAA2Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome
YPL182C_d5.832.72E-9YPL182C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W
YDR414C5.686.87E-9ERD1Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p)
YCR037C5.364.19E-8PHO87Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication
YHR142W5.344.65E-8CHS7Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress
YOL064C5.295.96E-8MET22Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway
YOR067C5.228.80E-8ALG8Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p
YJL211C_d5.141.37E-7YJL211C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2

GO enrichment analysis for SGTC_1729
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1241.12E-21SGTC_1730st033808 45.1 μMTimTec (Natural product derivative library)10451660.0833333
0.0881.06E-11SGTC_218alverine citrate 93.8 μMMiscellaneous217180.111111fatty acid desaturase (OLE1)
0.0863.93E-11SGTC_7450469-0705 296.0 μMChemDiv (Drug-like library)34867360.0714286mitochondrial processes
0.0864.36E-11SGTC_29919074669 71.4 μMChembridge (Drug-like library)173331090.0657895azole & statin
0.0864.52E-11SGTC_18845633444 25.0 μMMiscellaneous57242390.115942redox potentiating
0.0822.32E-10SGTC_258haloperidol 50.8 μMMiscellaneous35590.08fatty acid desaturase (OLE1)
0.0807.52E-10SGTC_700141-0289 43.3 μMChemDiv (Drug-like library)3524080.109375mitochondrial stress
0.0807.78E-10SGTC_31619100388 49.5 μMChembridge (Drug-like library)252363680.15942iron homeostasis
0.0781.77E-9SGTC_1593justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 54.9 μMTimTec (Pure natural product library)4428820.0657895redox potentiating
0.0781.86E-9SGTC_204PMA1.184 62.4 μMChemDiv (Drug-like library)22522130.0759494TRP & mitochondrial translation
0.0773.53E-9SGTC_28809042983 58.4 μMChembridge (Drug-like library)64647140.126761fatty acid desaturase (OLE1)
0.0773.73E-9SGTC_20665222451 176.0 μMChembridge (Fragment library)22811470.0909091heme biosynthesis & mitochondrial translocase
0.0764.08E-9SGTC_396dithiazanine 1.2 μMMiscellaneous105780.075
0.0741.10E-8SGTC_29819013952 39.0 μMChembridge (Drug-like library)29963440.166667
0.0741.29E-8SGTC_20464110841 106.0 μMChembridge (Fragment library)441196300.0909091iron homeostasis

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1728st03561433.79 μM0.37254915945546TimTec (Natural product derivative library)407.571125.25421
SGTC_20034023581200 μM0.22641516641307Chembridge (Fragment library)199.677262.51222cell wall signaling
SGTC_22657929201200 μM0.21265108Chembridge (Fragment library)222.309962.75633
SGTC_11463579-10237 μM0.19697X1146ChemDiv (Drug-like library)381.507835.48843
SGTC_315k915-0449187 μM0.19480520970215ChemDiv (Drug-like library)465.38226.19524
SGTC_3182910884949.47 μM0.19047617133045Chembridge (Drug-like library)267.322383.66112RPP1 & pyrimidine depletion
SGTC_3191911035249.47 μM0.19047617122424Chembridge (Drug-like library)267.322383.66112
SGTC_3037909102649.47 μM0.18840625236398Chembridge (Drug-like library)387.722343.71413NEO1-PIK1
SGTC_23569061756188.97 μM0.187520104043Chembridge (Fragment library)246.304921.46312
SGTC_2598perillyl alcohol100 μM0.18181810819Microsource (Natural product library)152.233442.41111