st034310

7-methoxy-2-(trichloromethyl)chromen-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1735
Screen concentration 56.8 μM
Source TimTec (Natural product derivative library)
PubChem CID 871153
SMILES COC1=CC2=C(C=C1)C(=O)C=C(O2)C(Cl)(Cl)Cl
Standardized SMILES COc1ccc2C(=O)C=C(Oc2c1)C(Cl)(Cl)Cl
Molecular weight 293.5305
ALogP 3.13
H-bond donor count 0
H-bond acceptor count 3
Response signature Golgi

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.58
% growth inhibition (Hom. pool) 6.23


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 871153
Download HIP data (tab-delimited text)  (excel)
Gene:AAR2(YBL074C)|FD-Score:4.65|P-value:1.70E-6|Clearance:0.3||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:ACP1(YKL192C)|FD-Score:-3.52|P-value:2.13E-4|Clearance:0||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:ALG2(YGL065C)|FD-Score:4.28|P-value:9.48E-6|Clearance:0.14||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:ARP2(YDL029W)|FD-Score:4.75|P-value:1.00E-6|Clearance:0.1||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:ARP7(YPR034W)|FD-Score:3.27|P-value:5.39E-4|Clearance:0.04||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CCT7(YJL111W)|FD-Score:4.13|P-value:1.78E-5|Clearance:0.07||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:ERG8(YMR220W)|FD-Score:3.39|P-value:3.51E-4|Clearance:0.06||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:HEM12(YDR047W)|FD-Score:-3.56|P-value:1.82E-4|Clearance:0||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:LSM2(YBL026W)|FD-Score:4.65|P-value:1.62E-6|Clearance:0.01||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM5(YER146W)|FD-Score:5.81|P-value:3.04E-9|Clearance:1.06||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MCM6(YGL201C)|FD-Score:-3.97|P-value:3.66E-5|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:MOT1(YPL082C)|FD-Score:4.31|P-value:8.14E-6|Clearance:0.03||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:MRD1(YPR112C)|FD-Score:-3.15|P-value:8.04E-4|Clearance:0||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:NAT2(YGR147C)|FD-Score:-3.51|P-value:2.24E-4|Clearance:0||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NUG1(YER006W)|FD-Score:-3.16|P-value:8.01E-4|Clearance:0||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:POL31(YJR006W)|FD-Score:4.06|P-value:2.40E-5|Clearance:0.14||SGD DESC:Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair Gene:PSF3(YOL146W)|FD-Score:3.45|P-value:2.78E-4|Clearance:0.06||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RET2(YFR051C)|FD-Score:4.34|P-value:7.00E-6|Clearance:0.03||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RFT1(YBL020W)|FD-Score:3.33|P-value:4.32E-4|Clearance:0.06||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RPL32(YBL092W)|FD-Score:3.76|P-value:8.65E-5|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:TAF9(YMR236W)|FD-Score:3.75|P-value:8.91E-5|Clearance:0.03||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:TSR4(YOL022C)|FD-Score:3.72|P-value:9.94E-5|Clearance:0.27||SGD DESC:Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 Gene:UBA1(YKL210W)|FD-Score:3.23|P-value:6.11E-4|Clearance:0.08||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:URB1(YKL014C)|FD-Score:-3.76|P-value:8.41E-5|Clearance:0||SGD DESC:Nucleolar protein required for the normal accumulation of 25S and 5.8S rRNAs, associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit Gene:UTP25(YIL091C)|FD-Score:3.92|P-value:4.34E-5|Clearance:0.17||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YCL041C(YCL041C_d)|FD-Score:3.15|P-value:8.14E-4|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W Gene:YOR146W(YOR146W_d)|FD-Score:-3.48|P-value:2.55E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPL142C(YPL142C_d)|FD-Score:-4.45|P-value:4.26E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:AAR2(YBL074C)|FD-Score:4.65|P-value:1.70E-6|Clearance:0.3||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:ACP1(YKL192C)|FD-Score:-3.52|P-value:2.13E-4|Clearance:0||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:ALG2(YGL065C)|FD-Score:4.28|P-value:9.48E-6|Clearance:0.14||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:ARP2(YDL029W)|FD-Score:4.75|P-value:1.00E-6|Clearance:0.1||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:ARP7(YPR034W)|FD-Score:3.27|P-value:5.39E-4|Clearance:0.04||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CCT7(YJL111W)|FD-Score:4.13|P-value:1.78E-5|Clearance:0.07||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:ERG8(YMR220W)|FD-Score:3.39|P-value:3.51E-4|Clearance:0.06||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:HEM12(YDR047W)|FD-Score:-3.56|P-value:1.82E-4|Clearance:0||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:LSM2(YBL026W)|FD-Score:4.65|P-value:1.62E-6|Clearance:0.01||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM5(YER146W)|FD-Score:5.81|P-value:3.04E-9|Clearance:1.06||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MCM6(YGL201C)|FD-Score:-3.97|P-value:3.66E-5|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:MOT1(YPL082C)|FD-Score:4.31|P-value:8.14E-6|Clearance:0.03||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:MRD1(YPR112C)|FD-Score:-3.15|P-value:8.04E-4|Clearance:0||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:NAT2(YGR147C)|FD-Score:-3.51|P-value:2.24E-4|Clearance:0||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NUG1(YER006W)|FD-Score:-3.16|P-value:8.01E-4|Clearance:0||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:POL31(YJR006W)|FD-Score:4.06|P-value:2.40E-5|Clearance:0.14||SGD DESC:Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair Gene:PSF3(YOL146W)|FD-Score:3.45|P-value:2.78E-4|Clearance:0.06||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RET2(YFR051C)|FD-Score:4.34|P-value:7.00E-6|Clearance:0.03||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RFT1(YBL020W)|FD-Score:3.33|P-value:4.32E-4|Clearance:0.06||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RPL32(YBL092W)|FD-Score:3.76|P-value:8.65E-5|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:TAF9(YMR236W)|FD-Score:3.75|P-value:8.91E-5|Clearance:0.03||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:TSR4(YOL022C)|FD-Score:3.72|P-value:9.94E-5|Clearance:0.27||SGD DESC:Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 Gene:UBA1(YKL210W)|FD-Score:3.23|P-value:6.11E-4|Clearance:0.08||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:URB1(YKL014C)|FD-Score:-3.76|P-value:8.41E-5|Clearance:0||SGD DESC:Nucleolar protein required for the normal accumulation of 25S and 5.8S rRNAs, associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit Gene:UTP25(YIL091C)|FD-Score:3.92|P-value:4.34E-5|Clearance:0.17||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YCL041C(YCL041C_d)|FD-Score:3.15|P-value:8.14E-4|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W Gene:YOR146W(YOR146W_d)|FD-Score:-3.48|P-value:2.55E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPL142C(YPL142C_d)|FD-Score:-4.45|P-value:4.26E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 871153
Download HOP data (tab-delimited text)  (excel)
Gene:AIM19(YIL087C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth Gene:AIM43(YPL099C)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALG8(YOR067C)|FD-Score:4.33|P-value:7.61E-6||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:APL4(YPR029C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport Gene:ARO3(YDR035W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG26(YLR189C)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy Gene:BZZ1(YHR114W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:CIS3(YJL158C)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family Gene:CMK2(YOL016C)|FD-Score:-4.25|P-value:1.07E-5||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:COG5(YNL051W)|FD-Score:4.5|P-value:3.44E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:6.42|P-value:6.86E-11||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:7.16|P-value:4.17E-13||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DIG1(YPL049C)|FD-Score:5.52|P-value:1.73E-8||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DOG1(YHR044C)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified Gene:DXO1(YDR370C_p)|FD-Score:4.35|P-value:6.86E-6||SGD DESC:Protein with decapping and 5′-3′ exoRNase activity; similar to Rai1p Gene:ELP4(YPL101W)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERV41(YML067C)|FD-Score:3.23|P-value:6.08E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:FAA3(YIL009W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FAT1(YBR041W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FCY1(YPR062W)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FMP27(YLR454W_p)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN4(YEL009C)|FD-Score:4.87|P-value:5.71E-7||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GIC2(YDR309C)|FD-Score:-4.45|P-value:4.21E-6||SGD DESC:Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region Gene:GND2(YGR256W)|FD-Score:4.4|P-value:5.34E-6||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:GRE2(YOL151W)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:GSP2(YOR185C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:GTR2(YGR163W)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HAP4(YKL109W)|FD-Score:-3.23|P-value:6.13E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HSP104(YLL026W)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress Gene:IRC13(YOR235W_d)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC25(YLR021W)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:IRS4(YKR019C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISU2(YOR226C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:KKQ8(YKL168C)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:LEO1(YOR123C)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LRO1(YNR008W)|FD-Score:-4.05|P-value:2.56E-5||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MPP6(YNR024W)|FD-Score:4.83|P-value:6.94E-7||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MSC7(YHR039C)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MUP1(YGR055W)|FD-Score:-3.12|P-value:9.01E-4||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:NNT1(YLR285W)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination Gene:NOP16(YER002W)|FD-Score:-3.81|P-value:6.83E-5||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:PNS1(YOR161C)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport Gene:POR1(YNL055C)|FD-Score:4.3|P-value:8.46E-6||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PRE9(YGR135W)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:PRM10(YJL108C)|FD-Score:-3.1|P-value:9.76E-4||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:PRX1(YBL064C)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress Gene:PTP3(YER075C)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:REC102(YLR329W)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination Gene:RGP1(YDR137W)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL13A(YDL082W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL23A(YBL087C)|FD-Score:-4.35|P-value:6.94E-6||SGD DESC:Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication Gene:RPL40A(YIL148W)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS30A(YLR287C-A)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RSA1(YPL193W)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:SAC6(YDR129C)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAM4(YPL273W)|FD-Score:-3.09|P-value:9.98E-4||SGD DESC:S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:SFC1(YJR095W)|FD-Score:-3.8|P-value:7.14E-5||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SIP1(YDR422C)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions Gene:SKI7(YOR076C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype Gene:SLM3(YDL033C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SRX1(YKL086W)|FD-Score:-3.97|P-value:3.57E-5||SGD DESC:Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress Gene:STV1(YMR054W)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SUL2(YLR092W)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SYP1(YCR030C)|FD-Score:4.55|P-value:2.64E-6||SGD DESC:Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization Gene:TKL1(YPR074C)|FD-Score:3.72|P-value:9.91E-5||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRI1(YMR233W)|FD-Score:5.92|P-value:1.65E-9||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:5.41|P-value:3.08E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.75|P-value:8.90E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UIP3(YAR027W)|FD-Score:-3.78|P-value:7.69E-5||SGD DESC:Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family Gene:URM1(YIL008W)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VEL1(YGL258W)|FD-Score:-3.83|P-value:6.34E-5||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPH1(YOR270C)|FD-Score:-4.57|P-value:2.38E-6||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS65(YLR322W_d)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:VTA1(YLR181C)|FD-Score:-4.9|P-value:4.83E-7||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:YBR220C(YBR220C_p)|FD-Score:3.74|P-value:9.33E-5||SGD DESC:Putative protein of unknown function; YBR220C is not an essential gene Gene:YDR476C(YDR476C_p)|FD-Score:6.66|P-value:1.33E-11||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YEL057C(YEL057C_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YER053C-A(YER053C-A_p)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YFR054C(YFR054C_d)|FD-Score:4.99|P-value:3.07E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL185C(YGL185C_p)|FD-Score:3.85|P-value:5.88E-5||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR111W(YGR111W_p)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YKL063C(YKL063C_p)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi Gene:YKR075C(YKR075C_p)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YML007C-A(YML007C-A_p)|FD-Score:-3.79|P-value:7.57E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR158C-A(YMR158C-A_p)|FD-Score:-3.16|P-value:7.77E-4||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene Gene:YMR172C-A(YMR172C-A_d)|FD-Score:5.68|P-value:6.62E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPQ1(YOL092W_p)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YPR170C(YPR170C_d)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YPR172W(YPR172W_p)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication Gene:AIM19(YIL087C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth Gene:AIM43(YPL099C)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALG8(YOR067C)|FD-Score:4.33|P-value:7.61E-6||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:APL4(YPR029C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport Gene:ARO3(YDR035W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG26(YLR189C)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy Gene:BZZ1(YHR114W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:CIS3(YJL158C)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family Gene:CMK2(YOL016C)|FD-Score:-4.25|P-value:1.07E-5||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:COG5(YNL051W)|FD-Score:4.5|P-value:3.44E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:6.42|P-value:6.86E-11||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:7.16|P-value:4.17E-13||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DIG1(YPL049C)|FD-Score:5.52|P-value:1.73E-8||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DOG1(YHR044C)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified Gene:DXO1(YDR370C_p)|FD-Score:4.35|P-value:6.86E-6||SGD DESC:Protein with decapping and 5′-3′ exoRNase activity; similar to Rai1p Gene:ELP4(YPL101W)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERV41(YML067C)|FD-Score:3.23|P-value:6.08E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:FAA3(YIL009W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FAT1(YBR041W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FCY1(YPR062W)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FMP27(YLR454W_p)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN4(YEL009C)|FD-Score:4.87|P-value:5.71E-7||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GIC2(YDR309C)|FD-Score:-4.45|P-value:4.21E-6||SGD DESC:Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region Gene:GND2(YGR256W)|FD-Score:4.4|P-value:5.34E-6||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:GRE2(YOL151W)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:GSP2(YOR185C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:GTR2(YGR163W)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HAP4(YKL109W)|FD-Score:-3.23|P-value:6.13E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HSP104(YLL026W)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress Gene:IRC13(YOR235W_d)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC25(YLR021W)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:IRS4(YKR019C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISU2(YOR226C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:KKQ8(YKL168C)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:LEO1(YOR123C)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LRO1(YNR008W)|FD-Score:-4.05|P-value:2.56E-5||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MPP6(YNR024W)|FD-Score:4.83|P-value:6.94E-7||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MSC7(YHR039C)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MUP1(YGR055W)|FD-Score:-3.12|P-value:9.01E-4||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:NNT1(YLR285W)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination Gene:NOP16(YER002W)|FD-Score:-3.81|P-value:6.83E-5||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:PNS1(YOR161C)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport Gene:POR1(YNL055C)|FD-Score:4.3|P-value:8.46E-6||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PRE9(YGR135W)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:PRM10(YJL108C)|FD-Score:-3.1|P-value:9.76E-4||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:PRX1(YBL064C)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress Gene:PTP3(YER075C)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:REC102(YLR329W)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination Gene:RGP1(YDR137W)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL13A(YDL082W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL23A(YBL087C)|FD-Score:-4.35|P-value:6.94E-6||SGD DESC:Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication Gene:RPL40A(YIL148W)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS30A(YLR287C-A)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RSA1(YPL193W)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:SAC6(YDR129C)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAM4(YPL273W)|FD-Score:-3.09|P-value:9.98E-4||SGD DESC:S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:SFC1(YJR095W)|FD-Score:-3.8|P-value:7.14E-5||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SIP1(YDR422C)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions Gene:SKI7(YOR076C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype Gene:SLM3(YDL033C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SRX1(YKL086W)|FD-Score:-3.97|P-value:3.57E-5||SGD DESC:Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress Gene:STV1(YMR054W)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SUL2(YLR092W)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SYP1(YCR030C)|FD-Score:4.55|P-value:2.64E-6||SGD DESC:Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization Gene:TKL1(YPR074C)|FD-Score:3.72|P-value:9.91E-5||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRI1(YMR233W)|FD-Score:5.92|P-value:1.65E-9||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:5.41|P-value:3.08E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.75|P-value:8.90E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UIP3(YAR027W)|FD-Score:-3.78|P-value:7.69E-5||SGD DESC:Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family Gene:URM1(YIL008W)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VEL1(YGL258W)|FD-Score:-3.83|P-value:6.34E-5||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPH1(YOR270C)|FD-Score:-4.57|P-value:2.38E-6||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS65(YLR322W_d)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:VTA1(YLR181C)|FD-Score:-4.9|P-value:4.83E-7||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:YBR220C(YBR220C_p)|FD-Score:3.74|P-value:9.33E-5||SGD DESC:Putative protein of unknown function; YBR220C is not an essential gene Gene:YDR476C(YDR476C_p)|FD-Score:6.66|P-value:1.33E-11||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YEL057C(YEL057C_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YER053C-A(YER053C-A_p)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YFR054C(YFR054C_d)|FD-Score:4.99|P-value:3.07E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL185C(YGL185C_p)|FD-Score:3.85|P-value:5.88E-5||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR111W(YGR111W_p)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YKL063C(YKL063C_p)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi Gene:YKR075C(YKR075C_p)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YML007C-A(YML007C-A_p)|FD-Score:-3.79|P-value:7.57E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR158C-A(YMR158C-A_p)|FD-Score:-3.16|P-value:7.77E-4||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene Gene:YMR172C-A(YMR172C-A_d)|FD-Score:5.68|P-value:6.62E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPQ1(YOL092W_p)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YPR170C(YPR170C_d)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YPR172W(YPR172W_p)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER146W5.813.04E-91.06LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YDL029W4.751.00E-60.10ARP2Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YBL026W4.651.62E-60.01LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YBL074C4.651.70E-60.30AAR2Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron
YFR051C4.347.00E-60.03RET2Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YPL082C4.318.14E-60.03MOT1Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA
YGL065C4.289.48E-60.14ALG2Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol
YJL111W4.131.78E-50.07CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YJR006W4.062.40E-50.14POL31Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair
YIL091C3.924.34E-50.17UTP25Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis
YBL092W3.768.65E-50.01RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YMR236W3.758.91E-50.03TAF9Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
YOL022C3.729.94E-50.27TSR4Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2
YOL146W3.452.78E-40.06PSF3Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YMR220W3.393.51E-40.06ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML071C7.164.17E-13COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YDR476C_p6.661.33E-11YDR476C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene
YNL041C6.426.86E-11COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YMR233W5.921.65E-9TRI1Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication
YMR172C-A_d5.686.62E-9YMR172C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPL049C5.521.73E-8DIG1MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p
YER090W5.413.08E-8TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YFR054C_d4.993.07E-7YFR054C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YEL009C4.875.71E-7GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YNR024W4.836.94E-7MPP6Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes
YBL064C4.741.09E-6PRX1Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress
YGL005C4.691.35E-6COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YLR021W4.691.38E-6IRC25Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
YCR030C4.552.64E-6SYP1Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization
YNL051W4.503.44E-6COG5Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments

GO enrichment analysis for SGTC_1735
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1541.49E-32SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.0649351Golgi
0.1483.98E-30SGTC_1671st016596 74.5 μMTimTec (Natural product derivative library)28291670.0821918
0.1455.13E-29SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.0625Golgi
0.1396.78E-27SGTC_624k048-0052 28.0 μMChemDiv (Drug-like library)68355310.0704225Golgi
0.1381.44E-26SGTC_2231micatex 6.9 μMMiscellaneous207370.0816327
0.1331.15E-24SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.047619Golgi
0.1331.53E-24SGTC_220964-0034 14.5 μMChemDiv (Drug-like library)1384020.1unfolded protein response
0.1315.15E-24SGTC_1078idebenone 36.1 μMMiscellaneous36860.125Golgi
0.1308.95E-24SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.078125
0.1301.62E-23SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.037037
0.1284.03E-23SGTC_22557941087 197.4 μMChembridge (Fragment library)9355610.0746269
0.1271.50E-22SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.125
0.1262.55E-22SGTC_1655st012546 55.1 μMTimTec (Natural product derivative library)7209850.175439
0.1255.77E-22SGTC_20415107442 45.3 μMChembridge (Fragment library)7930050.132075RSC complex & mRNA processing
0.1231.70E-21SGTC_15998-chlro-1-tetrahydronorharmanone 90.6 μMTimTec (Pure natural product library)41748400.0967742

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1727st0343099.9 μM0.789474870305TimTec (Natural product derivative library)293.530483.13403
SGTC_1867st05737264 μM0.581395688837TimTec (Natural product derivative library)312.316643.08705
SGTC_1722st0343043.65 μM0.5641034036148TimTec (Natural product derivative library)263.50453.1502mitochondrial processes
SGTC_1887st05764870.8 μM0.555556688672TimTec (Natural product derivative library)282.290663.10304
SGTC_1732st03430851.82 μM0.547619830119TimTec (Natural product derivative library)277.531083.63602
SGTC_15447-methoxy-4-methylcoumarin105 μM0.52381390807TimTec (Pure natural product library)190.19532.32903
SGTC_1723st0343072.55 μM0.52381867466TimTec (Natural product derivative library)297.949563.81402mitochondrial processes
SGTC_1866st05763864 μM0.468085688802TimTec (Natural product derivative library)312.316643.08705
SGTC_1912st06020990.8 μM0.468085688787TimTec (Natural product derivative library)220.221281.9204
SGTC_15427-hydroxy-3'-methoxyflavone49.7 μM0.450985393153TimTec (Pure natural product library)268.264082.87814