st036774

8-benzhydrylsulfanyl-1,3,7-trimethylpurine-2,6-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1736
Screen concentration 51.0 μM
Source TimTec (Natural product derivative library)
PubChem CID 1272247
SMILES CN1C2=C(N=C1SC(C3=CC=CC=C3)C4=CC=CC=C4)N(C(=O)N(C2=O)C)C
Standardized SMILES CN1C(=O)N(C)c2nc(SC(c3ccccc3)c4ccccc4)n(C)c2C1=O
Molecular weight 392.4741
ALogP 4.18
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 2.28
% growth inhibition (Hom. pool) -0.1


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1272247
Download HIP data (tab-delimited text)  (excel)
Gene:ARP2(YDL029W)|FD-Score:5.02|P-value:2.55E-7|Clearance:1.12||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:DNA2(YHR164C)|FD-Score:3.78|P-value:7.89E-5|Clearance:0.27||SGD DESC:Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress Gene:IFH1(YLR223C)|FD-Score:3.34|P-value:4.19E-4|Clearance:0.18||SGD DESC:Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing Gene:MET30(YIL046W)|FD-Score:3.16|P-value:7.82E-4|Clearance:0.12||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:NHP2(YDL208W)|FD-Score:-3.44|P-value:2.91E-4|Clearance:0||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:PGK1(YCR012W)|FD-Score:3.38|P-value:3.63E-4|Clearance:0.04||SGD DESC:3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis Gene:RRP4(YHR069C)|FD-Score:-3.37|P-value:3.71E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SMC4(YLR086W)|FD-Score:-3.26|P-value:5.64E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SSU72(YNL222W)|FD-Score:3.9|P-value:4.72E-5|Clearance:0.05||SGD DESC:Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo Gene:URB2(YJR041C)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.13||SGD DESC:Nucleolar protein required for normal metabolism of the rRNA primary transcript, proposed to be involved in ribosome biogenesis Gene:UTP25(YIL091C)|FD-Score:3.85|P-value:5.91E-5|Clearance:0.07||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:WRS1(YOL097C)|FD-Score:-3.64|P-value:1.35E-4|Clearance:0||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:ARP2(YDL029W)|FD-Score:5.02|P-value:2.55E-7|Clearance:1.12||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:DNA2(YHR164C)|FD-Score:3.78|P-value:7.89E-5|Clearance:0.27||SGD DESC:Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress Gene:IFH1(YLR223C)|FD-Score:3.34|P-value:4.19E-4|Clearance:0.18||SGD DESC:Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing Gene:MET30(YIL046W)|FD-Score:3.16|P-value:7.82E-4|Clearance:0.12||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:NHP2(YDL208W)|FD-Score:-3.44|P-value:2.91E-4|Clearance:0||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:PGK1(YCR012W)|FD-Score:3.38|P-value:3.63E-4|Clearance:0.04||SGD DESC:3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis Gene:RRP4(YHR069C)|FD-Score:-3.37|P-value:3.71E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SMC4(YLR086W)|FD-Score:-3.26|P-value:5.64E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SSU72(YNL222W)|FD-Score:3.9|P-value:4.72E-5|Clearance:0.05||SGD DESC:Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo Gene:URB2(YJR041C)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.13||SGD DESC:Nucleolar protein required for normal metabolism of the rRNA primary transcript, proposed to be involved in ribosome biogenesis Gene:UTP25(YIL091C)|FD-Score:3.85|P-value:5.91E-5|Clearance:0.07||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:WRS1(YOL097C)|FD-Score:-3.64|P-value:1.35E-4|Clearance:0||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1272247
Download HOP data (tab-delimited text)  (excel)
Gene:AIM19(YIL087C)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth Gene:ANT1(YPR128C)|FD-Score:7.04|P-value:9.38E-13||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ATG1(YGL180W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:BTS1(YPL069C)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDC10(YCR002C)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CEM1(YER061C)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:CMR3(YPR013C_p)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:ECI1(YLR284C)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:ESC2(YDR363W)|FD-Score:-5.25|P-value:7.80E-8||SGD DESC:Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member Gene:FSH2(YMR222C)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:GCN4(YEL009C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCR2(YNL199C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GEP5(YLR091W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GET3(YDL100C)|FD-Score:4.97|P-value:3.41E-7||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GLY1(YEL046C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GRX7(YBR014C)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication Gene:HDA2(YDR295C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance Gene:HNT3(YOR258W)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HO(YDL227C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p Gene:IPT1(YDR072C)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:IRC21(YMR073C)|FD-Score:4.28|P-value:9.40E-6||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:JJJ2(YJL162C)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein Gene:LAA1(YJL207C)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LDB16(YCL005W)|FD-Score:5.85|P-value:2.49E-9||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LRP1(YHR081W)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:MAM33(YIL070C)|FD-Score:-3.31|P-value:4.68E-4||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MCH5(YOR306C)|FD-Score:3.75|P-value:8.75E-5||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MET14(YKL001C)|FD-Score:5.97|P-value:1.20E-9||SGD DESC:Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Gene:MIC17(YMR002W)|FD-Score:5.1|P-value:1.70E-7||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MKC7(YDR144C)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p Gene:MNN5(YJL186W)|FD-Score:-3.1|P-value:9.63E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MSC7(YHR039C)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MUK1(YPL070W)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Cytoplasmic protein of unknown function containing a Vps9 domain; computational analysis of large-scale protein-protein interaction data suggests a possible role in transcriptional regulation Gene:MUM2(YBR057C)|FD-Score:-5.4|P-value:3.35E-8||SGD DESC:Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex Gene:MZM1(YDR493W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NRM1(YNR009W)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:NSR1(YGR159C)|FD-Score:-4.03|P-value:2.79E-5||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:NTG1(YAL015C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication Gene:OXP1(YKL215C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PPM1(YDR435C)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PRM9(YAR031W)|FD-Score:-3.9|P-value:4.90E-5||SGD DESC:Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family Gene:PRX1(YBL064C)|FD-Score:6.22|P-value:2.49E-10||SGD DESC:Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress Gene:PTP3(YER075C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:RAD4(YER162C)|FD-Score:5.34|P-value:4.65E-8||SGD DESC:Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins Gene:RAV1(YJR033C)|FD-Score:6.31|P-value:1.40E-10||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:REV1(YOR346W)|FD-Score:-3.81|P-value:7.06E-5||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RFU1(YLR073C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RMD9(YGL107C)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:ROG3(YFR022W)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication Gene:RPL20A(YMR242C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RRM3(YHR031C)|FD-Score:-3.85|P-value:5.91E-5||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:SET5(YHR207C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SLT2(YHR030C)|FD-Score:4.84|P-value:6.39E-7||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOP4(YJL192C)|FD-Score:4.82|P-value:7.16E-7||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SPO11(YHL022C)|FD-Score:5.25|P-value:7.51E-8||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SPR6(YER115C)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SSD1(YDR293C)|FD-Score:5.05|P-value:2.23E-7||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUR4(YLR372W)|FD-Score:9.26|P-value:1.02E-20||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:THP1(YOL072W)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TIR2(YOR010C)|FD-Score:3.72|P-value:9.89E-5||SGD DESC:Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis Gene:TPK2(YPL203W)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TSC3(YBR058C-A)|FD-Score:3.88|P-value:5.32E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUF1(YOR187W)|FD-Score:-3.22|P-value:6.30E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:URC2(YDR520C_p)|FD-Score:-3.9|P-value:4.75E-5||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VPH2(YKL119C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:WAR1(YML076C)|FD-Score:4.38|P-value:5.99E-6||SGD DESC:Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively Gene:YCL021W-A(YCL021W-A_p)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Putative protein of unknown function Gene:YDR090C(YDR090C_p)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Putative protein of unknown function Gene:YDR186C(YDR186C)|FD-Score:6.85|P-value:3.65E-12||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR476C(YDR476C_p)|FD-Score:5.76|P-value:4.19E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YER039C-A(YER039C-A_p)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YER187W(YER187W_p)|FD-Score:5.53|P-value:1.59E-8||SGD DESC:Putative protein of unknown function; induced in respiratory-deficient cells Gene:YFR035C(YFR035C_p)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGL010W(YGL010W_p)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGL185C(YGL185C_p)|FD-Score:7.54|P-value:2.28E-14||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHK8(YHR048W_p)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles Gene:YIL058W(YIL058W_d)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL165C(YIL165C_p)|FD-Score:4.7|P-value:1.30E-6||SGD DESC:Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene Gene:YIP3(YNL044W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p Gene:YKL044W(YKL044W_p)|FD-Score:4.38|P-value:6.07E-6||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL077W(YKL077W_p)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKL133C(YKL133C_p)|FD-Score:-3.24|P-value:6.05E-4||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YKL169C(YKL169C_d)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YMR181C(YMR181C_p)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication Gene:YMR265C(YMR265C_p)|FD-Score:3.76|P-value:8.46E-5||SGD DESC:Putative protein of unknown function Gene:YMR31(YFR049W)|FD-Score:4.85|P-value:6.13E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 Gene:YNL303W(YNL303W_d)|FD-Score:3.72|P-value:9.98E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR029W(YOR029W_d)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL071C(YPL071C_p)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL119C-A(YPL119C-A_p)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPL152W-A(YPL152W-A_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPR078C(YPR078C_p)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:ZRG17(YNR039C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency Gene:AIM19(YIL087C)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth Gene:ANT1(YPR128C)|FD-Score:7.04|P-value:9.38E-13||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ATG1(YGL180W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:BTS1(YPL069C)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDC10(YCR002C)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CEM1(YER061C)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:CMR3(YPR013C_p)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:ECI1(YLR284C)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:ESC2(YDR363W)|FD-Score:-5.25|P-value:7.80E-8||SGD DESC:Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member Gene:FSH2(YMR222C)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:GCN4(YEL009C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCR2(YNL199C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GEP5(YLR091W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GET3(YDL100C)|FD-Score:4.97|P-value:3.41E-7||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GLY1(YEL046C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GRX7(YBR014C)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication Gene:HDA2(YDR295C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance Gene:HNT3(YOR258W)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HO(YDL227C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p Gene:IPT1(YDR072C)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:IRC21(YMR073C)|FD-Score:4.28|P-value:9.40E-6||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:JJJ2(YJL162C)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein Gene:LAA1(YJL207C)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LDB16(YCL005W)|FD-Score:5.85|P-value:2.49E-9||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LRP1(YHR081W)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:MAM33(YIL070C)|FD-Score:-3.31|P-value:4.68E-4||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MCH5(YOR306C)|FD-Score:3.75|P-value:8.75E-5||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MET14(YKL001C)|FD-Score:5.97|P-value:1.20E-9||SGD DESC:Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Gene:MIC17(YMR002W)|FD-Score:5.1|P-value:1.70E-7||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MKC7(YDR144C)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p Gene:MNN5(YJL186W)|FD-Score:-3.1|P-value:9.63E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MSC7(YHR039C)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MUK1(YPL070W)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Cytoplasmic protein of unknown function containing a Vps9 domain; computational analysis of large-scale protein-protein interaction data suggests a possible role in transcriptional regulation Gene:MUM2(YBR057C)|FD-Score:-5.4|P-value:3.35E-8||SGD DESC:Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex Gene:MZM1(YDR493W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NRM1(YNR009W)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:NSR1(YGR159C)|FD-Score:-4.03|P-value:2.79E-5||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:NTG1(YAL015C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication Gene:OXP1(YKL215C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PPM1(YDR435C)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PRM9(YAR031W)|FD-Score:-3.9|P-value:4.90E-5||SGD DESC:Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family Gene:PRX1(YBL064C)|FD-Score:6.22|P-value:2.49E-10||SGD DESC:Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress Gene:PTP3(YER075C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:RAD4(YER162C)|FD-Score:5.34|P-value:4.65E-8||SGD DESC:Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins Gene:RAV1(YJR033C)|FD-Score:6.31|P-value:1.40E-10||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:REV1(YOR346W)|FD-Score:-3.81|P-value:7.06E-5||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RFU1(YLR073C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RMD9(YGL107C)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:ROG3(YFR022W)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication Gene:RPL20A(YMR242C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RRM3(YHR031C)|FD-Score:-3.85|P-value:5.91E-5||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:SET5(YHR207C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SLT2(YHR030C)|FD-Score:4.84|P-value:6.39E-7||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOP4(YJL192C)|FD-Score:4.82|P-value:7.16E-7||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SPO11(YHL022C)|FD-Score:5.25|P-value:7.51E-8||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SPR6(YER115C)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SSD1(YDR293C)|FD-Score:5.05|P-value:2.23E-7||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUR4(YLR372W)|FD-Score:9.26|P-value:1.02E-20||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:THP1(YOL072W)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TIR2(YOR010C)|FD-Score:3.72|P-value:9.89E-5||SGD DESC:Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis Gene:TPK2(YPL203W)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TSC3(YBR058C-A)|FD-Score:3.88|P-value:5.32E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUF1(YOR187W)|FD-Score:-3.22|P-value:6.30E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:URC2(YDR520C_p)|FD-Score:-3.9|P-value:4.75E-5||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VPH2(YKL119C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:WAR1(YML076C)|FD-Score:4.38|P-value:5.99E-6||SGD DESC:Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively Gene:YCL021W-A(YCL021W-A_p)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Putative protein of unknown function Gene:YDR090C(YDR090C_p)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Putative protein of unknown function Gene:YDR186C(YDR186C)|FD-Score:6.85|P-value:3.65E-12||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR476C(YDR476C_p)|FD-Score:5.76|P-value:4.19E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YER039C-A(YER039C-A_p)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YER187W(YER187W_p)|FD-Score:5.53|P-value:1.59E-8||SGD DESC:Putative protein of unknown function; induced in respiratory-deficient cells Gene:YFR035C(YFR035C_p)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGL010W(YGL010W_p)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGL185C(YGL185C_p)|FD-Score:7.54|P-value:2.28E-14||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHK8(YHR048W_p)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles Gene:YIL058W(YIL058W_d)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL165C(YIL165C_p)|FD-Score:4.7|P-value:1.30E-6||SGD DESC:Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene Gene:YIP3(YNL044W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p Gene:YKL044W(YKL044W_p)|FD-Score:4.38|P-value:6.07E-6||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL077W(YKL077W_p)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKL133C(YKL133C_p)|FD-Score:-3.24|P-value:6.05E-4||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YKL169C(YKL169C_d)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YMR181C(YMR181C_p)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication Gene:YMR265C(YMR265C_p)|FD-Score:3.76|P-value:8.46E-5||SGD DESC:Putative protein of unknown function Gene:YMR31(YFR049W)|FD-Score:4.85|P-value:6.13E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 Gene:YNL303W(YNL303W_d)|FD-Score:3.72|P-value:9.98E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR029W(YOR029W_d)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL071C(YPL071C_p)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL119C-A(YPL119C-A_p)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPL152W-A(YPL152W-A_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPR078C(YPR078C_p)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:ZRG17(YNR039C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL029W5.022.55E-71.12ARP2Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YNL222W3.904.72E-50.05SSU72Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo
YIL091C3.855.91E-50.07UTP25Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis
YHR164C3.787.89E-50.27DNA2Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress
YJR041C3.512.22E-40.13URB2Nucleolar protein required for normal metabolism of the rRNA primary transcript, proposed to be involved in ribosome biogenesis
YCR012W3.383.63E-40.04PGK13-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis
YLR223C3.344.19E-40.18IFH1Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
YIL046W3.167.82E-40.12MET30F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
YGR158C3.040.001190.01MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YDR021W3.030.001220.10FAL1Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases
YJR023C_d2.940.001670.07YJR023C_dPutative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing
YGL130W2.870.002080.07CEG1Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
YML105C2.800.002590.02SEC65Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
YOR272W2.780.002730.00YTM1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats
YPR161C2.770.002770.00SGV1Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR372W9.261.02E-20SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YGL185C_p7.542.28E-14YGL185C_pPutative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YPR128C7.049.38E-13ANT1Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation
YDR186C6.853.65E-12YDR186CPutative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YJR033C6.311.40E-10RAV1Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
YBL064C6.222.49E-10PRX1Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress
YKL001C5.971.20E-9MET14Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism
YCL005W5.852.49E-9LDB16Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
YDR476C_p5.764.19E-9YDR476C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene
YER187W_p5.531.59E-8YER187W_pPutative protein of unknown function; induced in respiratory-deficient cells
YER162C5.344.65E-8RAD4Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins
YHL022C5.257.51E-8SPO11Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation
YMR002W5.101.70E-7MIC17Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
YDR293C5.052.23E-7SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YDL100C4.973.41E-7GET3Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_1736
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1225.31E-21SGTC_2740felodipine 17.1 μMMiscellaneous33330.0779221
0.1187.53E-20SGTC_5481436-0017 110.0 μMChemDiv (Drug-like library)96771370.1TSC3-RPN4
0.1024.79E-15SGTC_1726st035556 14.6 μMTimTec (Natural product derivative library)59194390.0923077
0.1001.20E-14SGTC_1982st072467 71.9 μMTimTec (Natural product derivative library)31635330.1
0.0978.31E-14SGTC_1735st034310 56.8 μMTimTec (Natural product derivative library)8711530.0882353Golgi
0.0952.36E-13SGTC_487nimodipine 119.0 μMMiscellaneous44970.0813954
0.0938.85E-13SGTC_14103909-8106 77.9 μMChemDiv (Drug-like library)10392100.0705882
0.0881.62E-11SGTC_1731st035964 63.0 μMTimTec (Natural product derivative library)67395750.0519481
0.0857.15E-11SGTC_13321480-0653 17.5 μMChemDiv (Drug-like library)57494380.123077TSC3-RPN4
0.0849.09E-11SGTC_33369143619 71.4 μMChembridge (Drug-like library)455957300.0793651
0.0841.15E-10SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.057971TSC3-RPN4
0.0814.68E-10SGTC_8830850-0245 24.6 μMChemDiv (Drug-like library)68232290.0875cell wall
0.0815.36E-10SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.0786517
0.0791.09E-9SGTC_33429147791 14.5 μMChembridge (Drug-like library)18381680.0987654
0.0782.12E-9SGTC_9221866-0035 480.0 μMChemDiv (Drug-like library)31561690.08excess fatty acid

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_228caffeine993.06 μM0.3958332519Miscellaneous194.1906-0.103
SGTC_1791st04831356.1 μM0.3809521204976TimTec (Natural product derivative library)356.37582.86505
SGTC_1792st04577343.6 μM0.3684212919270TimTec (Natural product derivative library)458.451122.50809
SGTC_1748st03745553.6 μM0.366667580690TimTec (Natural product derivative library)339.176683.02403
SGTC_23759072683200 μM0.35087716798029Chembridge (Fragment library)261.27980.54703mitochondrial processes
SGTC_2348902254333.42 μM0.3392863162678Chembridge (Fragment library)275.306380.8303mitochondrial processes
SGTC_1740st03828613.22 μM0.333333716786TimTec (Natural product derivative library)266.319461.29213
SGTC_1749st03793254.2 μM0.323077594497TimTec (Natural product derivative library)369.202663.33704
SGTC_23387999974200 μM0.315789315925Chembridge (Fragment library)234.254460.96303
SGTC_1743st03760646.38 μM0.311475590142TimTec (Natural product derivative library)326.392882.88403