st035555

(E)-1-(4-ethoxyphenyl)-3-(2-fluorophenyl)prop-2-en-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1738
Screen concentration 74.0 μM
Source TimTec (Natural product derivative library)
PubChem CID 5331140
SMILES CCOC1=CC=C(C=C1)C(=O)C=CC2=CC=CC=C2F
Standardized SMILES CCOc1ccc(cc1)C(=O)C=Cc2ccccc2F
Molecular weight 270.2982
ALogP 4.24
H-bond donor count 0
H-bond acceptor count 3
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.95
% growth inhibition (Hom. pool) 10.44


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5331140
Download HIP data (tab-delimited text)  (excel)
Gene:CDC8(YJR057W)|FD-Score:-6.22|P-value:2.52E-10|Clearance:0||SGD DESC:Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p Gene:CDC9(YDL164C)|FD-Score:3.38|P-value:3.63E-4|Clearance:0.11||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:CEG1(YGL130W)|FD-Score:4.09|P-value:2.12E-5|Clearance:0.14||SGD DESC:Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:ESF2(YNR054C)|FD-Score:3.76|P-value:8.37E-5|Clearance:0.14||SGD DESC:Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome Gene:GPI2(YPL076W)|FD-Score:3.27|P-value:5.37E-4|Clearance:0.05||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein Gene:HTB1(YDR224C)|FD-Score:-4.41|P-value:5.09E-6|Clearance:0||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:KRI1(YNL308C)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.02||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:KRR1(YCL059C)|FD-Score:3.87|P-value:5.49E-5|Clearance:0.1||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:LSM3(YLR438C-A)|FD-Score:3.12|P-value:8.94E-4|Clearance:0.01||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM5(YER146W)|FD-Score:4.47|P-value:3.83E-6|Clearance:0.35||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:PDS5(YMR076C)|FD-Score:3.95|P-value:3.87E-5|Clearance:0.08||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PRE10(YOR362C)|FD-Score:3.22|P-value:6.42E-4|Clearance:0.08||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PWP2(YCR057C)|FD-Score:-3.14|P-value:8.43E-4|Clearance:0||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPL25(YOL127W)|FD-Score:3.63|P-value:1.43E-4|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPP1(YHR062C)|FD-Score:3.12|P-value:9.16E-4|Clearance:0.02||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSC58(YLR033W)|FD-Score:-3.29|P-value:4.96E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC9(YML127W)|FD-Score:-4.39|P-value:5.63E-6|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC63(YOR254C)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.14||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SHQ1(YIL104C)|FD-Score:-3.55|P-value:1.91E-4|Clearance:0||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:SLN1(YIL147C)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.05||SGD DESC:Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators Gene:SSU72(YNL222W)|FD-Score:4.44|P-value:4.48E-6|Clearance:0.35||SGD DESC:Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo Gene:UBC1(YDR177W)|FD-Score:3.09|P-value:9.84E-4|Clearance:0.06||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YAE1(YJR067C)|FD-Score:-3.34|P-value:4.20E-4|Clearance:0||SGD DESC:Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:YDR413C(YDR413C_d)|FD-Score:3.14|P-value:8.56E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YKL083W(YKL083W_d)|FD-Score:-3.17|P-value:7.66E-4|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YPL142C(YPL142C_d)|FD-Score:-3.61|P-value:1.53E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:CDC8(YJR057W)|FD-Score:-6.22|P-value:2.52E-10|Clearance:0||SGD DESC:Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p Gene:CDC9(YDL164C)|FD-Score:3.38|P-value:3.63E-4|Clearance:0.11||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:CEG1(YGL130W)|FD-Score:4.09|P-value:2.12E-5|Clearance:0.14||SGD DESC:Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:ESF2(YNR054C)|FD-Score:3.76|P-value:8.37E-5|Clearance:0.14||SGD DESC:Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome Gene:GPI2(YPL076W)|FD-Score:3.27|P-value:5.37E-4|Clearance:0.05||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein Gene:HTB1(YDR224C)|FD-Score:-4.41|P-value:5.09E-6|Clearance:0||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:KRI1(YNL308C)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.02||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:KRR1(YCL059C)|FD-Score:3.87|P-value:5.49E-5|Clearance:0.1||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:LSM3(YLR438C-A)|FD-Score:3.12|P-value:8.94E-4|Clearance:0.01||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM5(YER146W)|FD-Score:4.47|P-value:3.83E-6|Clearance:0.35||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:PDS5(YMR076C)|FD-Score:3.95|P-value:3.87E-5|Clearance:0.08||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PRE10(YOR362C)|FD-Score:3.22|P-value:6.42E-4|Clearance:0.08||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PWP2(YCR057C)|FD-Score:-3.14|P-value:8.43E-4|Clearance:0||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPL25(YOL127W)|FD-Score:3.63|P-value:1.43E-4|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPP1(YHR062C)|FD-Score:3.12|P-value:9.16E-4|Clearance:0.02||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSC58(YLR033W)|FD-Score:-3.29|P-value:4.96E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC9(YML127W)|FD-Score:-4.39|P-value:5.63E-6|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC63(YOR254C)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.14||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SHQ1(YIL104C)|FD-Score:-3.55|P-value:1.91E-4|Clearance:0||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:SLN1(YIL147C)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.05||SGD DESC:Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators Gene:SSU72(YNL222W)|FD-Score:4.44|P-value:4.48E-6|Clearance:0.35||SGD DESC:Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo Gene:UBC1(YDR177W)|FD-Score:3.09|P-value:9.84E-4|Clearance:0.06||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YAE1(YJR067C)|FD-Score:-3.34|P-value:4.20E-4|Clearance:0||SGD DESC:Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:YDR413C(YDR413C_d)|FD-Score:3.14|P-value:8.56E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YKL083W(YKL083W_d)|FD-Score:-3.17|P-value:7.66E-4|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YPL142C(YPL142C_d)|FD-Score:-3.61|P-value:1.53E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5331140
Download HOP data (tab-delimited text)  (excel)
Gene:ADE16(YLR028C)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:AEP1(YMR064W)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AIM34(YMR003W)|FD-Score:5.45|P-value:2.48E-8||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss Gene:ARA1(YBR149W)|FD-Score:-3.21|P-value:6.74E-4||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ARG1(YOL058W)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate Gene:ARO1(YDR127W)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATG1(YGL180W)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:BDH1(YAL060W)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:CDC26(YFR036W)|FD-Score:5.17|P-value:1.18E-7||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CHD1(YER164W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:COQ6(YGR255C)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS) Gene:COX16(YJL003W)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:DAL80(YKR034W)|FD-Score:-3.47|P-value:2.62E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DAL81(YIR023W)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAP2(YHR028C)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DUN1(YDL101C)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:DXO1(YDR370C_p)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Protein with decapping and 5′-3′ exoRNase activity; similar to Rai1p Gene:EXO1(YOR033C)|FD-Score:5.28|P-value:6.29E-8||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:GBP2(YCL011C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GCV2(YMR189W)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm Gene:GEP5(YLR091W)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GLE2(YER107C)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GMC2(YLR445W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GPD1(YDL022W)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:HAP3(YBL021C)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding Gene:HIS3(YOR202W)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p Gene:INO1(YJL153C)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:ISY1(YJR050W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:KKQ8(YKL168C)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:KTR7(YIL085C)|FD-Score:3.77|P-value:8.29E-5||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:LTE1(YAL024C)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAE1(YKL029C)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MDH3(YDL078C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Peroxisomal malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle Gene:MNN10(YDR245W)|FD-Score:4.39|P-value:5.66E-6||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MRF1(YGL143C)|FD-Score:-3.37|P-value:3.69E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MRPL44(YMR225C)|FD-Score:4.29|P-value:9.06E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPS35(YGR165W)|FD-Score:-3.24|P-value:6.02E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MSE1(YOL033W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:MTC1(YJL123C)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 Gene:MTG2(YHR168W)|FD-Score:3.85|P-value:5.85E-5||SGD DESC:Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly Gene:MUP3(YHL036W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NMA111(YNL123W)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NUP84(YDL116W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:ORM1(YGR038W)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:OSH2(YDL019C)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PCL5(YHR071W)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity Gene:PGD1(YGL025C)|FD-Score:7.31|P-value:1.36E-13||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO84(YML123C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PIN4(YBL051C)|FD-Score:-3.15|P-value:8.26E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PIR1(YKL164C)|FD-Score:-5.02|P-value:2.59E-7||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:PSP2(YML017W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PUT4(YOR348C)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells Gene:RAD17(YOR368W)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RAD52(YML032C)|FD-Score:4.48|P-value:3.68E-6||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:RDH54(YBR073W)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p Gene:RDS1(YCR106W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:ROM1(YGR070W)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPN4(YDL020C)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT5(YFR032C_p)|FD-Score:-8.34|P-value:3.84E-17||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:RSM22(YKL155C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RTS1(YOR014W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SDP1(YIL113W)|FD-Score:9.18|P-value:2.11E-20||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SDS24(YBR214W)|FD-Score:4.95|P-value:3.77E-7||SGD DESC:Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication Gene:SHC1(YER096W)|FD-Score:5.75|P-value:4.55E-9||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SKG1(YKR100C)|FD-Score:5.65|P-value:8.13E-9||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SKI7(YOR076C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype Gene:SLX9(YGR081C)|FD-Score:-6.59|P-value:2.20E-11||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SOD1(YJR104C)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPC1(YJR010C-A)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SPS19(YNL202W)|FD-Score:3.73|P-value:9.50E-5||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:TDH2(YJR009C)|FD-Score:3.25|P-value:5.67E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:THI72(YOR192C)|FD-Score:4.37|P-value:6.20E-6||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:THP1(YOL072W)|FD-Score:5.34|P-value:4.74E-8||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TMA10(YLR327C)|FD-Score:-3.81|P-value:7.06E-5||SGD DESC:Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress Gene:TRP2(YER090W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSC3(YBR058C-A)|FD-Score:20.6|P-value:3.45E-94||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VBA5(YKR105C_p)|FD-Score:-3.14|P-value:8.50E-4||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:YAL065C(YAL065C_p)|FD-Score:5.01|P-value:2.74E-7||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YCR090C(YCR090C_p)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDL177C(YDL177C_p)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR061W(YDR061W_p)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance Gene:YDR249C(YDR249C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein of unknown function Gene:YEL008W(YEL008W_d)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Hypothetical protein predicted to be involved in metabolism Gene:YEL067C(YEL067C_p)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YER034W(YER034W_p)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YER067C-A(YER067C-A_d)|FD-Score:3.85|P-value:6.02E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Gene:YER084W(YER084W_p)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YET1(YKL065C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YHM2(YMR241W)|FD-Score:7.29|P-value:1.56E-13||SGD DESC:Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome Gene:YIL001W(YIL001W_p)|FD-Score:5.96|P-value:1.29E-9||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YIR042C(YIR042C_p)|FD-Score:7.72|P-value:6.03E-15||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YJL120W(YJL120W_d)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YLR001C(YLR001C_p)|FD-Score:5.02|P-value:2.59E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YMR175W-A(YMR175W-A_p)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Putative protein of unknown function Gene:YMR304C-A(YMR304C-A_d)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YNL057W(YNL057W_d)|FD-Score:4.88|P-value:5.35E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR062C(YNR062C_p)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Putative membrane protein of unknown function Gene:YPL245W(YPL245W_p)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YPR127W(YPR127W)|FD-Score:-4.25|P-value:1.07E-5||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:ZTA1(YBR046C)|FD-Score:-4.93|P-value:4.18E-7||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin Gene:ADE16(YLR028C)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:AEP1(YMR064W)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AIM34(YMR003W)|FD-Score:5.45|P-value:2.48E-8||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss Gene:ARA1(YBR149W)|FD-Score:-3.21|P-value:6.74E-4||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ARG1(YOL058W)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate Gene:ARO1(YDR127W)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATG1(YGL180W)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:BDH1(YAL060W)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:CDC26(YFR036W)|FD-Score:5.17|P-value:1.18E-7||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CHD1(YER164W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:COQ6(YGR255C)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS) Gene:COX16(YJL003W)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:DAL80(YKR034W)|FD-Score:-3.47|P-value:2.62E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DAL81(YIR023W)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAP2(YHR028C)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DUN1(YDL101C)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:DXO1(YDR370C_p)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Protein with decapping and 5′-3′ exoRNase activity; similar to Rai1p Gene:EXO1(YOR033C)|FD-Score:5.28|P-value:6.29E-8||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:GBP2(YCL011C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GCV2(YMR189W)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm Gene:GEP5(YLR091W)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GLE2(YER107C)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GMC2(YLR445W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GPD1(YDL022W)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:HAP3(YBL021C)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding Gene:HIS3(YOR202W)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p Gene:INO1(YJL153C)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:ISY1(YJR050W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:KKQ8(YKL168C)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:KTR7(YIL085C)|FD-Score:3.77|P-value:8.29E-5||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:LTE1(YAL024C)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAE1(YKL029C)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MDH3(YDL078C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Peroxisomal malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle Gene:MNN10(YDR245W)|FD-Score:4.39|P-value:5.66E-6||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MRF1(YGL143C)|FD-Score:-3.37|P-value:3.69E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MRPL44(YMR225C)|FD-Score:4.29|P-value:9.06E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPS35(YGR165W)|FD-Score:-3.24|P-value:6.02E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MSE1(YOL033W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:MTC1(YJL123C)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 Gene:MTG2(YHR168W)|FD-Score:3.85|P-value:5.85E-5||SGD DESC:Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly Gene:MUP3(YHL036W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NMA111(YNL123W)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NUP84(YDL116W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:ORM1(YGR038W)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:OSH2(YDL019C)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PCL5(YHR071W)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity Gene:PGD1(YGL025C)|FD-Score:7.31|P-value:1.36E-13||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO84(YML123C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PIN4(YBL051C)|FD-Score:-3.15|P-value:8.26E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PIR1(YKL164C)|FD-Score:-5.02|P-value:2.59E-7||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:PSP2(YML017W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PUT4(YOR348C)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells Gene:RAD17(YOR368W)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RAD52(YML032C)|FD-Score:4.48|P-value:3.68E-6||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:RDH54(YBR073W)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p Gene:RDS1(YCR106W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:ROM1(YGR070W)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPN4(YDL020C)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT5(YFR032C_p)|FD-Score:-8.34|P-value:3.84E-17||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:RSM22(YKL155C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RTS1(YOR014W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SDP1(YIL113W)|FD-Score:9.18|P-value:2.11E-20||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SDS24(YBR214W)|FD-Score:4.95|P-value:3.77E-7||SGD DESC:Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication Gene:SHC1(YER096W)|FD-Score:5.75|P-value:4.55E-9||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SKG1(YKR100C)|FD-Score:5.65|P-value:8.13E-9||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SKI7(YOR076C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype Gene:SLX9(YGR081C)|FD-Score:-6.59|P-value:2.20E-11||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SOD1(YJR104C)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPC1(YJR010C-A)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SPS19(YNL202W)|FD-Score:3.73|P-value:9.50E-5||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:TDH2(YJR009C)|FD-Score:3.25|P-value:5.67E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:THI72(YOR192C)|FD-Score:4.37|P-value:6.20E-6||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:THP1(YOL072W)|FD-Score:5.34|P-value:4.74E-8||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TMA10(YLR327C)|FD-Score:-3.81|P-value:7.06E-5||SGD DESC:Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress Gene:TRP2(YER090W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSC3(YBR058C-A)|FD-Score:20.6|P-value:3.45E-94||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VBA5(YKR105C_p)|FD-Score:-3.14|P-value:8.50E-4||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:YAL065C(YAL065C_p)|FD-Score:5.01|P-value:2.74E-7||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YCR090C(YCR090C_p)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDL177C(YDL177C_p)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR061W(YDR061W_p)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance Gene:YDR249C(YDR249C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein of unknown function Gene:YEL008W(YEL008W_d)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Hypothetical protein predicted to be involved in metabolism Gene:YEL067C(YEL067C_p)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YER034W(YER034W_p)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YER067C-A(YER067C-A_d)|FD-Score:3.85|P-value:6.02E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Gene:YER084W(YER084W_p)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YET1(YKL065C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YHM2(YMR241W)|FD-Score:7.29|P-value:1.56E-13||SGD DESC:Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome Gene:YIL001W(YIL001W_p)|FD-Score:5.96|P-value:1.29E-9||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YIR042C(YIR042C_p)|FD-Score:7.72|P-value:6.03E-15||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YJL120W(YJL120W_d)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YLR001C(YLR001C_p)|FD-Score:5.02|P-value:2.59E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YMR175W-A(YMR175W-A_p)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Putative protein of unknown function Gene:YMR304C-A(YMR304C-A_d)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YNL057W(YNL057W_d)|FD-Score:4.88|P-value:5.35E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR062C(YNR062C_p)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Putative membrane protein of unknown function Gene:YPL245W(YPL245W_p)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YPR127W(YPR127W)|FD-Score:-4.25|P-value:1.07E-5||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:ZTA1(YBR046C)|FD-Score:-4.93|P-value:4.18E-7||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER146W4.473.83E-60.35LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YNL222W4.444.48E-60.35SSU72Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo
YGL130W4.092.12E-50.14CEG1Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
YMR076C3.953.87E-50.08PDS5Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis
YCL059C3.875.49E-50.10KRR1Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit
YNR054C3.768.37E-50.14ESF2Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome
YOL127W3.631.43E-40.04RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YIL147C3.591.66E-40.05SLN1Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators
YOR254C3.541.97E-40.14SEC63Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YNL308C3.403.38E-40.02KRI1Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p
YDL164C3.383.63E-40.11CDC9DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination
YPL076W3.275.37E-40.05GPI2Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein
YOR362C3.226.42E-40.08PRE10Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YDR413C_d3.148.56E-40.01YDR413C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing
YLR438C-A3.128.94E-40.01LSM3Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A20.603.45E-94TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YIL113W9.182.11E-20SDP1Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock
YIR042C_p7.726.03E-15YIR042C_pPutative protein of unknown function; YIR042C is a non-essential gene
YGL025C7.311.36E-13PGD1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
YMR241W7.291.56E-13YHM2Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome
YIL001W_p5.961.29E-9YIL001W_pPutative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm
YER096W5.754.55E-9SHC1Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication
YKR100C5.658.13E-9SKG1Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell
YMR003W5.452.48E-8AIM34Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss
YOL072W5.344.74E-8THP1Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding
YOR033C5.286.29E-8EXO15'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress
YFR036W5.171.18E-7CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YLR001C_p5.022.59E-7YLR001C_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated
YAL065C_p5.012.74E-7YAL065C_pPutative protein of unknown function; has homology to FLO1; possible pseudogene
YBR214W4.953.77E-7SDS24Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1738
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3665.79E-187SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.272727TSC3-RPN4
0.3591.07E-179SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.030303TSC3-RPN4
0.3527.77E-172SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.133333TSC3-RPN4
0.3302.72E-150SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.372549TSC3-RPN4
0.3271.65E-147SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.314815TSC3-RPN4
0.3161.18E-136SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0985916TSC3-RPN4
0.3147.36E-135SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0615385TSC3-RPN4
0.3115.05E-133SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.15873TSC3-RPN4
0.3016.57E-124SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.169014
0.3011.51E-123SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0555556TSC3-RPN4
0.2924.20E-116SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.189189TSC3-RPN4
0.2882.10E-113SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.118644TSC3-RPN4
0.2795.25E-106SGTC_1813st051246 8.6 μMTimTec (Natural product derivative library)52919590.333333TSC3-RPN4
0.2731.23E-101SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.241379
0.2734.66E-101SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.123288TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1726st03555614.58 μM0.6190485919439TimTec (Natural product derivative library)260.6907034.57102
SGTC_1737st03555769.7 μM0.5652175716916TimTec (Natural product derivative library)286.75284.69802
SGTC_19284'-methoxychalcone40.3 μM0.456522641818TimTec (Natural product derivative library)238.281163.68502
SGTC_24984'-Methoxychalcone54.63 μM0.456522641818Microsource (Natural product library)238.281163.68502RPP1 & pyrimidine depletion
SGTC_1691st01993384.6 μM0.3877556176082TimTec (Natural product derivative library)236.308344.67401
SGTC_1603st00204567.8 μM0.3725495887985TimTec (Natural product derivative library)295.1357635.23602TSC3-RPN4
SGTC_26092,3,4-trihydroxy-4'-ethoxybenzophenone70 μM0.3725496708739Microsource (Natural product library)274.268662.8435redox potentiating
SGTC_2998906676271.43 μM0.3684214945196Chembridge (Drug-like library)297.348363.54713RPP1 & pyrimidine depletion
SGTC_3140909424349.47 μM0.36666719291112Chembridge (Drug-like library)342.4072233.32204
SGTC_26044'-hydroxychalcone11.32 μM0.3617025282362Microsource (Natural product library)224.254583.4612