3553-0389

N-(4-chloro-2-methylphenyl)thiophene-2-sulfonamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_174
Screen concentration 74.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 738071
SMILES CC1=C(C=CC(=C1)Cl)NS(=O)(=O)C2=CC=CS2
Standardized SMILES Cc1cc(Cl)ccc1NS(=O)(=O)c2cccs2
Molecular weight 287.7856
ALogP 3.25
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 17.98
% growth inhibition (Hom. pool) 8.37


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 738071
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.07||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CCT7(YJL111W)|FD-Score:4.31|P-value:8.22E-6|Clearance:0.4||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC13(YDL220C)|FD-Score:-3.12|P-value:9.17E-4|Clearance:0||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:CEG1(YGL130W)|FD-Score:3.31|P-value:4.69E-4|Clearance:0.04||SGD DESC:Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:DRE2(YKR071C)|FD-Score:3.27|P-value:5.33E-4|Clearance:0.02||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:FCP1(YMR277W)|FD-Score:-3.59|P-value:1.68E-4|Clearance:0||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:FIP1(YJR093C)|FD-Score:3.32|P-value:4.57E-4|Clearance:0.01||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:MMS21(YEL019C)|FD-Score:-3.13|P-value:8.71E-4|Clearance:0||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:NUP49(YGL172W)|FD-Score:3.25|P-value:5.71E-4|Clearance:0.01||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:NUT2(YPR168W)|FD-Score:-3.52|P-value:2.17E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:POL3(YDL102W)|FD-Score:3.81|P-value:7.07E-5|Clearance:0.08||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:RPB11(YOL005C)|FD-Score:3.24|P-value:5.92E-4|Clearance:0.12||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RSC6(YCR052W)|FD-Score:3.12|P-value:9.12E-4|Clearance:0.14||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RTS2(YOR077W)|FD-Score:3.44|P-value:2.91E-4|Clearance:0.12||SGD DESC:Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication Gene:SAR1(YPL218W)|FD-Score:-3.17|P-value:7.72E-4|Clearance:0||SGD DESC:GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport Gene:UTP8(YGR128C)|FD-Score:3.72|P-value:9.80E-5|Clearance:0.21||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YJL015C(YJL015C_d)|FD-Score:4.2|P-value:1.31E-5|Clearance:0.4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Gene:ACT1(YFL039C)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.07||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CCT7(YJL111W)|FD-Score:4.31|P-value:8.22E-6|Clearance:0.4||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC13(YDL220C)|FD-Score:-3.12|P-value:9.17E-4|Clearance:0||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:CEG1(YGL130W)|FD-Score:3.31|P-value:4.69E-4|Clearance:0.04||SGD DESC:Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:DRE2(YKR071C)|FD-Score:3.27|P-value:5.33E-4|Clearance:0.02||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:FCP1(YMR277W)|FD-Score:-3.59|P-value:1.68E-4|Clearance:0||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:FIP1(YJR093C)|FD-Score:3.32|P-value:4.57E-4|Clearance:0.01||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:MMS21(YEL019C)|FD-Score:-3.13|P-value:8.71E-4|Clearance:0||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:NUP49(YGL172W)|FD-Score:3.25|P-value:5.71E-4|Clearance:0.01||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:NUT2(YPR168W)|FD-Score:-3.52|P-value:2.17E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:POL3(YDL102W)|FD-Score:3.81|P-value:7.07E-5|Clearance:0.08||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:RPB11(YOL005C)|FD-Score:3.24|P-value:5.92E-4|Clearance:0.12||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RSC6(YCR052W)|FD-Score:3.12|P-value:9.12E-4|Clearance:0.14||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RTS2(YOR077W)|FD-Score:3.44|P-value:2.91E-4|Clearance:0.12||SGD DESC:Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication Gene:SAR1(YPL218W)|FD-Score:-3.17|P-value:7.72E-4|Clearance:0||SGD DESC:GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport Gene:UTP8(YGR128C)|FD-Score:3.72|P-value:9.80E-5|Clearance:0.21||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YJL015C(YJL015C_d)|FD-Score:4.2|P-value:1.31E-5|Clearance:0.4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 738071
Download HOP data (tab-delimited text)  (excel)
Gene:ACF2(YLR144C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress Gene:AIM46(YHR199C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:BUR2(YLR226W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II Gene:CAM1(YPL048W)|FD-Score:-4.31|P-value:8.10E-6||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CDC73(YLR418C)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CKI1(YLR133W)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CTF8(YHR191C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DST1(YGL043W)|FD-Score:6.7|P-value:1.08E-11||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:EGD2(YHR193C)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:ELM1(YKL048C)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ERG4(YGL012W)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:EST3(YIL009C-A)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Component of the telomerase holoenzyme, involved in telomere replication Gene:FYV4(YHR059W)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:HUL5(YGL141W)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD Gene:IRC13(YOR235W_d)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC18(YJL037W)|FD-Score:3.77|P-value:8.11E-5||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:LEU9(YOR108W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:MDM38(YOL027C)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MEP2(YNL142W)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MRP10(YDL045W-A)|FD-Score:3.93|P-value:4.24E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs Gene:MRPL38(YKL170W)|FD-Score:6.13|P-value:4.50E-10||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPS8(YMR158W)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTG1(YMR097C)|FD-Score:4.85|P-value:6.12E-7||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NDE2(YDL085W)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain Gene:NMA111(YNL123W)|FD-Score:4.55|P-value:2.73E-6||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:OCA1(YNL099C)|FD-Score:6.99|P-value:1.35E-12||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OCA5(YHL029C_p)|FD-Score:-3.93|P-value:4.24E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OPI1(YHL020C)|FD-Score:4.63|P-value:1.84E-6||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:OPI9(YLR338W_d)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PEX10(YDR265W)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders Gene:PHO5(YBR093C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 Gene:PTH2(YBL057C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PUF2(YPR042C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:QDR3(YBR043C)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:RAD17(YOR368W)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:REV7(YIL139C)|FD-Score:-4.43|P-value:4.79E-6||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RIM9(YMR063W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPL34A(YER056C-A)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPL9A(YGL147C)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPS7B(YNL096C)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SAE2(YGL175C)|FD-Score:5.93|P-value:1.53E-9||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SGS1(YMR190C)|FD-Score:-5.47|P-value:2.29E-8||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SHR5(YOL110W)|FD-Score:3.78|P-value:7.96E-5||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SPT4(YGR063C)|FD-Score:4.36|P-value:6.64E-6||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SSN3(YPL042C)|FD-Score:6.13|P-value:4.49E-10||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:THO2(YNL139C)|FD-Score:5.2|P-value:1.01E-7||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TOS6(YNL300W_p)|FD-Score:4.42|P-value:4.89E-6||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TRI1(YMR233W)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TUL1(YKL034W)|FD-Score:-3.15|P-value:8.07E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:TUM1(YOR251C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:TYW3(YGL050W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:UBC11(YOR339C)|FD-Score:-3.82|P-value:6.67E-5||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:VFA1(YER128W)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VPS51(YKR020W)|FD-Score:4.95|P-value:3.80E-7||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS65(YLR322W_d)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:VPS9(YML097C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YBL053W(YBL053W_d)|FD-Score:4.85|P-value:6.06E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR277C(YBR277C_d)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCR051W(YCR051W_p)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDL119C(YDL119C_p)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YLR118C(YLR118C)|FD-Score:-5.14|P-value:1.37E-7||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR149C(YLR149C_p)|FD-Score:4.06|P-value:2.42E-5||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YLR460C(YLR460C_p)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YNL010W(YNL010W_p)|FD-Score:-4.32|P-value:7.95E-6||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YOL013W-B(YOL013W-B_d)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the long terminal repeat (LTR) of a Ty1 element Gene:YOR309C(YOR309C_d)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPL114W(YPL114W_d)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPL182C(YPL182C_d)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:ACF2(YLR144C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress Gene:AIM46(YHR199C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:BUR2(YLR226W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II Gene:CAM1(YPL048W)|FD-Score:-4.31|P-value:8.10E-6||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CDC73(YLR418C)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CKI1(YLR133W)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CTF8(YHR191C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DST1(YGL043W)|FD-Score:6.7|P-value:1.08E-11||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:EGD2(YHR193C)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:ELM1(YKL048C)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ERG4(YGL012W)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:EST3(YIL009C-A)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Component of the telomerase holoenzyme, involved in telomere replication Gene:FYV4(YHR059W)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:HUL5(YGL141W)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD Gene:IRC13(YOR235W_d)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC18(YJL037W)|FD-Score:3.77|P-value:8.11E-5||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:LEU9(YOR108W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:MDM38(YOL027C)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MEP2(YNL142W)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MRP10(YDL045W-A)|FD-Score:3.93|P-value:4.24E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs Gene:MRPL38(YKL170W)|FD-Score:6.13|P-value:4.50E-10||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPS8(YMR158W)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTG1(YMR097C)|FD-Score:4.85|P-value:6.12E-7||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NDE2(YDL085W)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain Gene:NMA111(YNL123W)|FD-Score:4.55|P-value:2.73E-6||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:OCA1(YNL099C)|FD-Score:6.99|P-value:1.35E-12||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OCA5(YHL029C_p)|FD-Score:-3.93|P-value:4.24E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OPI1(YHL020C)|FD-Score:4.63|P-value:1.84E-6||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:OPI9(YLR338W_d)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PEX10(YDR265W)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders Gene:PHO5(YBR093C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 Gene:PTH2(YBL057C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PUF2(YPR042C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:QDR3(YBR043C)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:RAD17(YOR368W)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:REV7(YIL139C)|FD-Score:-4.43|P-value:4.79E-6||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RIM9(YMR063W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPL34A(YER056C-A)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPL9A(YGL147C)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPS7B(YNL096C)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SAE2(YGL175C)|FD-Score:5.93|P-value:1.53E-9||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SGS1(YMR190C)|FD-Score:-5.47|P-value:2.29E-8||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SHR5(YOL110W)|FD-Score:3.78|P-value:7.96E-5||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SPT4(YGR063C)|FD-Score:4.36|P-value:6.64E-6||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SSN3(YPL042C)|FD-Score:6.13|P-value:4.49E-10||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:THO2(YNL139C)|FD-Score:5.2|P-value:1.01E-7||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TOS6(YNL300W_p)|FD-Score:4.42|P-value:4.89E-6||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TRI1(YMR233W)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TUL1(YKL034W)|FD-Score:-3.15|P-value:8.07E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:TUM1(YOR251C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:TYW3(YGL050W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:UBC11(YOR339C)|FD-Score:-3.82|P-value:6.67E-5||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:VFA1(YER128W)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VPS51(YKR020W)|FD-Score:4.95|P-value:3.80E-7||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS65(YLR322W_d)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:VPS9(YML097C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YBL053W(YBL053W_d)|FD-Score:4.85|P-value:6.06E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR277C(YBR277C_d)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCR051W(YCR051W_p)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDL119C(YDL119C_p)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YLR118C(YLR118C)|FD-Score:-5.14|P-value:1.37E-7||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR149C(YLR149C_p)|FD-Score:4.06|P-value:2.42E-5||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YLR460C(YLR460C_p)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YNL010W(YNL010W_p)|FD-Score:-4.32|P-value:7.95E-6||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YOL013W-B(YOL013W-B_d)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the long terminal repeat (LTR) of a Ty1 element Gene:YOR309C(YOR309C_d)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPL114W(YPL114W_d)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPL182C(YPL182C_d)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL111W4.318.22E-60.40CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YJL015C_d4.201.31E-50.40YJL015C_dDubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W
YDL102W3.817.07E-50.08POL3Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
YGR128C3.729.80E-50.21UTP8Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YFL039C3.512.21E-40.07ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YOR077W3.442.91E-40.12RTS2Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication
YJR093C3.324.57E-40.01FIP1Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p
YGL130W3.314.69E-40.04CEG1Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
YKR071C3.275.33E-40.02DRE2Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
YGL172W3.255.71E-40.01NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YOL005C3.245.92E-40.12RPB11RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit
YCR052W3.129.12E-40.14RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YOR287C2.980.001450.05RRP36Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit
YLR198C_d2.930.001690.11YLR198C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W
YLR075W2.820.002438.78E-4RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL099C6.991.35E-12OCA1Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA
YGL043W6.701.08E-11DST1General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
YPL042C6.134.49E-10SSN3Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression
YKL170W6.134.50E-10MRPL38Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress
YGL175C5.931.53E-9SAE2Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
YNL139C5.201.01E-7THO2Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids
YKR020W4.953.80E-7VPS51Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p
YBL053W_d4.856.06E-7YBL053W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR097C4.856.12E-7MTG1Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals
YIL009C-A4.661.59E-6EST3Component of the telomerase holoenzyme, involved in telomere replication
YHL020C4.631.84E-6OPI1Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance
YNL123W4.552.73E-6NMA111Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
YHR059W4.503.33E-6FYV4Protein of unknown function, required for survival upon exposure to K1 killer toxin
YNL300W_p4.424.89E-6TOS6_pGlycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid
YGR063C4.366.64E-6SPT4Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions

GO enrichment analysis for SGTC_174
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0764.83E-9SGTC_1753793-3120 23.7 μMChemDiv (Drug-like library)53222970.084507
0.0664.63E-7SGTC_21836420625 130.5 μMChembridge (Fragment library)28976190.0882353mitochondrial processes
0.0649.83E-7SGTC_10184259-0013 28.1 μMChemDiv (Drug-like library)68012620.147059
0.0622.06E-6SGTC_30889117578 49.5 μMChembridge (Drug-like library)6783410.118644amide catabolism
0.0622.23E-6SGTC_14954469-0029 237.0 μMChemDiv (Drug-like library)7936800.213115
0.0612.32E-6SGTC_430570-0105 72.5 μMChemDiv (Drug-like library)11140460.181818ERG2
0.0604.43E-6SGTC_7083448-5400 146.0 μMChemDiv (Drug-like library)34621950.11236
0.0604.69E-6SGTC_1812st052057 4.3 μMTimTec (Natural product derivative library)36454180.0229885calcium & mitochondrial duress
0.0589.20E-6SGTC_1788fluconazole 20.0 μMMiscellaneous33650.0606061azole & statin
0.0571.33E-5SGTC_1731273-0061 2.7 μMChemDiv (Drug-like library)28474140.078125mitochondrial stress
0.0561.91E-5SGTC_28839059145 45.5 μMChembridge (Drug-like library)164765100.102941
0.0552.68E-5SGTC_1711st032263 62.8 μMTimTec (Natural product derivative library)39589790.0657895
0.0542.83E-5SGTC_9791426-2828 121.0 μMChemDiv (Drug-like library)7478720.245614
0.0526.01E-5SGTC_11554088-0394 48.2 μMChemDiv (Drug-like library)23262480.0625
0.0527.27E-5SGTC_5283492-0019 172.0 μMChemDiv (Drug-like library)28692620.0704225calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3246913546032.98 μM0.47058841446951Chembridge (Drug-like library)329.7743233.65715
SGTC_12580671-004357.1 μM0.4583334298767ChemDiv (Drug-like library)296.772522.72124
SGTC_3076911618749.47 μM0.33928627241210Chembridge (Drug-like library)327.783263.20925
SGTC_2838900268771.43 μM0.3220346457060Chembridge (Drug-like library)357.877063.64713fatty acid desaturase (OLE1)
SGTC_2917797910353.79 μM0.3214292980565Chembridge (Drug-like library)327.783263.07225
SGTC_2834900175558.44 μM0.32989575Chembridge (Drug-like library)376.289163.28215
SGTC_2961908329032.55 μM0.29629617741490Chembridge (Drug-like library)295.784463.47513
SGTC_13501495-0454146 μM0.2857141069057ChemDiv (Drug-like library)350.843043.92615
SGTC_3346915825443.58 μM0.28571417639500Chembridge (Drug-like library)311.783863.31414
SGTC_3434130-282729.8 μM0.2830191118244ChemDiv (Drug-like library)354.6118834.51614