st037937

N-(1,3-benzodioxol-5-yl)-2-[7-[(3-chlorophenyl)methyl]-1,3-dimethyl-2,6-dioxopurin-8-yl]sulfanylacetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1744
Screen concentration 38.9 μM
Source TimTec (Natural product derivative library)
PubChem CID 1016152
SMILES CN1C2=C(C(=O)N(C1=O)C)N(C(=N2)SCC(=O)NC3=CC4=C(C=C3)OCO4)CC5=CC(=CC=C5)Cl
Standardized SMILES CN1C(=O)N(C)c2nc(SCC(=O)Nc3ccc4OCOc4c3)n(Cc5cccc(Cl)c5)c2C1=O
Molecular weight 513.9534
ALogP 3.64
H-bond donor count 1
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.06
% growth inhibition (Hom. pool) -0.01


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1016152
Download HIP data (tab-delimited text)  (excel)
Gene:ATP16(YDL004W)|FD-Score:3.88|P-value:5.18E-5|Clearance:0.4||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:FIP1(YJR093C)|FD-Score:-4.11|P-value:1.97E-5|Clearance:0||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:HTB1(YDR224C)|FD-Score:4.79|P-value:8.27E-7|Clearance:0.4||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:NHP2(YDL208W)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.15||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:NNF1(YJR112W)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.02||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:ORC6(YHR118C)|FD-Score:4.2|P-value:1.31E-5|Clearance:0.4||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:PGA1(YNL158W)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.1||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:PRO3(YER023W)|FD-Score:4.73|P-value:1.13E-6|Clearance:0.4||SGD DESC:Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis Gene:RDS3(YPR094W)|FD-Score:4.51|P-value:3.24E-6|Clearance:0.4||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:RFC4(YOL094C)|FD-Score:-3.1|P-value:9.74E-4|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RRP14(YKL082C)|FD-Score:3.49|P-value:2.46E-4|Clearance:0.16||SGD DESC:Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family Gene:RRP42(YDL111C)|FD-Score:-3.39|P-value:3.44E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:SPP2(YOR148C)|FD-Score:-3.5|P-value:2.28E-4|Clearance:0||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:STU1(YBL034C)|FD-Score:-3.48|P-value:2.52E-4|Clearance:0||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:USB1(YLR132C)|FD-Score:4.28|P-value:9.55E-6|Clearance:0.4||SGD DESC:Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants Gene:VRG4(YGL225W)|FD-Score:5.16|P-value:1.23E-7|Clearance:0.4||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YOR102W(YOR102W_d)|FD-Score:4.03|P-value:2.78E-5|Clearance:0.4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Gene:ATP16(YDL004W)|FD-Score:3.88|P-value:5.18E-5|Clearance:0.4||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:FIP1(YJR093C)|FD-Score:-4.11|P-value:1.97E-5|Clearance:0||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:HTB1(YDR224C)|FD-Score:4.79|P-value:8.27E-7|Clearance:0.4||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:NHP2(YDL208W)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.15||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:NNF1(YJR112W)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.02||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:ORC6(YHR118C)|FD-Score:4.2|P-value:1.31E-5|Clearance:0.4||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:PGA1(YNL158W)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.1||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:PRO3(YER023W)|FD-Score:4.73|P-value:1.13E-6|Clearance:0.4||SGD DESC:Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis Gene:RDS3(YPR094W)|FD-Score:4.51|P-value:3.24E-6|Clearance:0.4||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:RFC4(YOL094C)|FD-Score:-3.1|P-value:9.74E-4|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RRP14(YKL082C)|FD-Score:3.49|P-value:2.46E-4|Clearance:0.16||SGD DESC:Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family Gene:RRP42(YDL111C)|FD-Score:-3.39|P-value:3.44E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:SPP2(YOR148C)|FD-Score:-3.5|P-value:2.28E-4|Clearance:0||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:STU1(YBL034C)|FD-Score:-3.48|P-value:2.52E-4|Clearance:0||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:USB1(YLR132C)|FD-Score:4.28|P-value:9.55E-6|Clearance:0.4||SGD DESC:Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants Gene:VRG4(YGL225W)|FD-Score:5.16|P-value:1.23E-7|Clearance:0.4||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YOR102W(YOR102W_d)|FD-Score:4.03|P-value:2.78E-5|Clearance:0.4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1016152
Download HOP data (tab-delimited text)  (excel)
Gene:ADE8(YDR408C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AIM23(YJL131C)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss Gene:AMN1(YBR158W)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:APL1(YJR005W)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:APM3(YBR288C)|FD-Score:-3.5|P-value:2.37E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:ARF1(YDL192W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ASI1(YMR119W)|FD-Score:-3.97|P-value:3.62E-5||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATG23(YLR431C)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:ATG29(YPL166W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:CIT2(YCR005C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; CIT2 has a paralog, CIT1, that arose from the whole genome duplication Gene:COX7(YMR256C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CUP2(YGL166W)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DID4(YKL002W)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:GLG2(YJL137C)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GPB1(YOR371C)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:HNT1(YDL125C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HSP104(YLL026W)|FD-Score:-5.46|P-value:2.43E-8||SGD DESC:Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress Gene:HST1(YOL068C)|FD-Score:4.73|P-value:1.15E-6||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:ISC10(YER180C)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:KAR4(YCL055W)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:KIP3(YGL216W)|FD-Score:4.62|P-value:1.88E-6||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning Gene:LIF1(YGL090W)|FD-Score:5.05|P-value:2.24E-7||SGD DESC:Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein Gene:LIN1(YHR156C)|FD-Score:5.1|P-value:1.73E-7||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MAM1(YER106W)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MBF1(YOR298C-A)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress Gene:MDM35(YKL053C-A)|FD-Score:4.95|P-value:3.75E-7||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MOS1(YCL057C-A)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MRPL4(YLR439W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRPS35(YGR165W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MSN5(YDR335W)|FD-Score:3.8|P-value:7.29E-5||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MSS1(YMR023C)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:MSS51(YLR203C)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:MTC1(YJL123C)|FD-Score:4.61|P-value:2.04E-6||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 Gene:MXR1(YER042W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NAP1(YKR048C)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NMD4(YLR363C)|FD-Score:-5.51|P-value:1.80E-8||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NPR2(YEL062W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:NUP133(YKR082W)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:PHO13(YDL236W)|FD-Score:4.46|P-value:4.07E-6||SGD DESC:Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity Gene:PRM3(YPL192C)|FD-Score:4.78|P-value:8.73E-7||SGD DESC:Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body Gene:PXL1(YKR090W)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RAD16(YBR114W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RBK1(YCR036W)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Putative ribokinase Gene:RDL1(YOR285W)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RIM13(YMR154C)|FD-Score:4.44|P-value:4.58E-6||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RPL6B(YLR448W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:4.99|P-value:2.95E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:SCO1(YBR037C)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SHM2(YLR058C)|FD-Score:-4.29|P-value:8.98E-6||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SLX9(YGR081C)|FD-Score:4.41|P-value:5.10E-6||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SSA3(YBL075C)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SUR1(YPL057C)|FD-Score:6.75|P-value:7.36E-12||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:SWI5(YDR146C)|FD-Score:3.75|P-value:8.97E-5||SGD DESC:Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication Gene:TDA5(YLR426W_p)|FD-Score:7.22|P-value:2.68E-13||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TRX3(YCR083W)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:VAC7(YNL054W)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VAM7(YGL212W)|FD-Score:4.47|P-value:3.97E-6||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:YBR016W(YBR016W)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YDL114W(YDL114W_p)|FD-Score:4.43|P-value:4.72E-6||SGD DESC:Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene Gene:YER186C(YER186C_p)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Putative protein of unknown function Gene:YGR093W(YGR093W_p)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YGR291C(YGR291C_d)|FD-Score:4.5|P-value:3.39E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL054W(YIL054W_p)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YIL086C(YIL086C_d)|FD-Score:5.98|P-value:1.08E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIP5(YGL161C)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJR149W(YJR149W_p)|FD-Score:3.68|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR001C(YLR001C_p)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR211C(YLR211C_p)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YMR001C-A(YMR001C-A_p)|FD-Score:4.55|P-value:2.65E-6||SGD DESC:Putative protein of unknown function Gene:YMR075C-A(YMR075C-A_d)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YMR122C(YMR122C_d)|FD-Score:-3.12|P-value:9.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR160W(YMR160W_p)|FD-Score:3.89|P-value:5.02E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YMR230W-A(YMR230W-A_p)|FD-Score:3.76|P-value:8.66E-5||SGD DESC:Putative protein of unknown function Gene:YMR31(YFR049W)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 Gene:YNL092W(YNL092W_p)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene Gene:YNR063W(YNR063W_p)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YPL225W(YPL225W)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPS1(YLR120C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YSW1(YBR148W)|FD-Score:4.28|P-value:9.24E-6||SGD DESC:Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane Gene:ADE8(YDR408C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AIM23(YJL131C)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss Gene:AMN1(YBR158W)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:APL1(YJR005W)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:APM3(YBR288C)|FD-Score:-3.5|P-value:2.37E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:ARF1(YDL192W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ASI1(YMR119W)|FD-Score:-3.97|P-value:3.62E-5||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATG23(YLR431C)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:ATG29(YPL166W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:CIT2(YCR005C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; CIT2 has a paralog, CIT1, that arose from the whole genome duplication Gene:COX7(YMR256C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CUP2(YGL166W)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DID4(YKL002W)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:GLG2(YJL137C)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GPB1(YOR371C)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:HNT1(YDL125C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HSP104(YLL026W)|FD-Score:-5.46|P-value:2.43E-8||SGD DESC:Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress Gene:HST1(YOL068C)|FD-Score:4.73|P-value:1.15E-6||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:ISC10(YER180C)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:KAR4(YCL055W)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:KIP3(YGL216W)|FD-Score:4.62|P-value:1.88E-6||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning Gene:LIF1(YGL090W)|FD-Score:5.05|P-value:2.24E-7||SGD DESC:Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein Gene:LIN1(YHR156C)|FD-Score:5.1|P-value:1.73E-7||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MAM1(YER106W)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MBF1(YOR298C-A)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress Gene:MDM35(YKL053C-A)|FD-Score:4.95|P-value:3.75E-7||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MOS1(YCL057C-A)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MRPL4(YLR439W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRPS35(YGR165W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MSN5(YDR335W)|FD-Score:3.8|P-value:7.29E-5||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MSS1(YMR023C)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:MSS51(YLR203C)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:MTC1(YJL123C)|FD-Score:4.61|P-value:2.04E-6||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 Gene:MXR1(YER042W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NAP1(YKR048C)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NMD4(YLR363C)|FD-Score:-5.51|P-value:1.80E-8||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NPR2(YEL062W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:NUP133(YKR082W)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:PHO13(YDL236W)|FD-Score:4.46|P-value:4.07E-6||SGD DESC:Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity Gene:PRM3(YPL192C)|FD-Score:4.78|P-value:8.73E-7||SGD DESC:Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body Gene:PXL1(YKR090W)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RAD16(YBR114W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RBK1(YCR036W)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Putative ribokinase Gene:RDL1(YOR285W)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RIM13(YMR154C)|FD-Score:4.44|P-value:4.58E-6||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RPL6B(YLR448W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:4.99|P-value:2.95E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:SCO1(YBR037C)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SHM2(YLR058C)|FD-Score:-4.29|P-value:8.98E-6||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SLX9(YGR081C)|FD-Score:4.41|P-value:5.10E-6||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SSA3(YBL075C)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SUR1(YPL057C)|FD-Score:6.75|P-value:7.36E-12||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:SWI5(YDR146C)|FD-Score:3.75|P-value:8.97E-5||SGD DESC:Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication Gene:TDA5(YLR426W_p)|FD-Score:7.22|P-value:2.68E-13||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TRX3(YCR083W)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:VAC7(YNL054W)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VAM7(YGL212W)|FD-Score:4.47|P-value:3.97E-6||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:YBR016W(YBR016W)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YDL114W(YDL114W_p)|FD-Score:4.43|P-value:4.72E-6||SGD DESC:Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene Gene:YER186C(YER186C_p)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Putative protein of unknown function Gene:YGR093W(YGR093W_p)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YGR291C(YGR291C_d)|FD-Score:4.5|P-value:3.39E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL054W(YIL054W_p)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YIL086C(YIL086C_d)|FD-Score:5.98|P-value:1.08E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIP5(YGL161C)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJR149W(YJR149W_p)|FD-Score:3.68|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR001C(YLR001C_p)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR211C(YLR211C_p)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YMR001C-A(YMR001C-A_p)|FD-Score:4.55|P-value:2.65E-6||SGD DESC:Putative protein of unknown function Gene:YMR075C-A(YMR075C-A_d)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YMR122C(YMR122C_d)|FD-Score:-3.12|P-value:9.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR160W(YMR160W_p)|FD-Score:3.89|P-value:5.02E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YMR230W-A(YMR230W-A_p)|FD-Score:3.76|P-value:8.66E-5||SGD DESC:Putative protein of unknown function Gene:YMR31(YFR049W)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 Gene:YNL092W(YNL092W_p)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene Gene:YNR063W(YNR063W_p)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YPL225W(YPL225W)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPS1(YLR120C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YSW1(YBR148W)|FD-Score:4.28|P-value:9.24E-6||SGD DESC:Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL225W5.161.23E-70.40VRG4Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi
YDR224C4.798.27E-70.40HTB1Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
YER023W4.731.13E-60.40PRO3Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis
YPR094W4.513.24E-60.40RDS3Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance
YLR132C4.289.55E-60.40USB1Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants
YHR118C4.201.31E-50.40ORC6Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p
YOR102W_d4.032.78E-50.40YOR102W_dDubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex
YDL004W3.885.18E-50.40ATP16Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
YKL082C3.492.46E-40.16RRP14Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family
YDL208W3.324.45E-40.15NHP2Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing
YJR112W3.187.46E-40.02NNF1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YNL158W3.167.98E-40.10PGA1Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes
YDR331W3.060.001110.01GPI8ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog
YMR005W3.040.001170.04TAF4TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate
YDL153C3.000.001330.03SAS10Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR426W_p7.222.68E-13TDA5_pPutative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele
YPL057C6.757.36E-12SUR1Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
YIL086C_d5.981.08E-9YIL086C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YHR156C5.101.73E-7LIN1Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication
YGL090W5.052.24E-7LIF1Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein
YBL072C4.992.95E-7RPS8AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication
YKL053C-A4.953.75E-7MDM35Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
YPL192C4.788.73E-7PRM3Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body
YOL068C4.731.15E-6HST1NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance
YGL216W4.621.88E-6KIP3Kinesin-related motor protein involved in mitotic spindle positioning
YJL123C4.612.04E-6MTC1Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1
YMR001C-A_p4.552.65E-6YMR001C-A_pPutative protein of unknown function
YGR291C_d4.503.39E-6YGR291C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YGL212W4.473.97E-6VAM7Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
YDL236W4.464.07E-6PHO13Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity

GO enrichment analysis for SGTC_1744
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0656.75E-7SGTC_2496diffractaic acid 79.8 μMMicrosource (Natural product library)948700.0707071
0.0543.80E-5SGTC_25534-methylesculetin 85.1 μMMicrosource (Natural product library)53195020.0930233
0.0534.85E-5SGTC_1832st054113 55.0 μMTimTec (Natural product derivative library)12512670.0934579
0.0518.79E-5SGTC_29859022375 71.4 μMChembridge (Drug-like library)49393350.168421RPP1 & pyrimidine depletion
0.0501.32E-4SGTC_15892',5'-dimethoxyflavone 47.2 μMTimTec (Pure natural product library)6886670.108696
0.0491.64E-4SGTC_24495812982 45.8 μMMiscellaneous7761850.153846
0.0491.91E-4SGTC_391gliotoxin 8.3 μMMiscellaneous62230.0841122
0.0491.95E-4SGTC_9551216-0110 33.0 μMChemDiv (Drug-like library)36197190.0927835sphingolipid biosynthesis & PDR1
0.0473.34E-4SGTC_31029121669 49.5 μMChembridge (Drug-like library)419050720.0816327
0.0464.08E-4SGTC_29137966556 36.6 μMChembridge (Drug-like library)29765160.14705960S ribosome export
0.0464.40E-4SGTC_266formosanin C 406.0 nMMiscellaneous735970.0408163sphingolipid biosynthesis & PDR1
0.0455.26E-4SGTC_11293453-1384 223.0 μMChemDiv (Drug-like library)10844520.173913
0.0446.30E-4SGTC_459nigericin 15.3 μMICCB bioactive library342300.0492958
0.0446.55E-4SGTC_490717-0965 32.1 μMChemDiv (Drug-like library)58893490.160S ribosome export
0.0420.00121SGTC_2251lansoprazole 556.0 μMNIH Clinical Collection38830.0825688

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1748st03745553.6 μM0.527778580690TimTec (Natural product derivative library)339.176683.02403
SGTC_1749st03793254.2 μM0.357143594497TimTec (Natural product derivative library)369.202663.33704
SGTC_3089911742549.47 μM0.354439401657Chembridge (Drug-like library)297.348363.81613
SGTC_1784st03933436.3 μM0.3535353496984TimTec (Natural product derivative library)551.663723.7110
SGTC_1742st03823528.89 μM0.333333564103TimTec (Natural product derivative library)380.827583.73103mitochondrial stress
SGTC_2782773636971.43 μM0.3253012950836Chembridge (Drug-like library)335.805263.52814
SGTC_3053909590349.47 μM0.30952417174018Chembridge (Drug-like library)317.766843.39313Golgi
SGTC_1736st03677451 μM0.3012051272247TimTec (Natural product derivative library)392.47414.17904
SGTC_3289911991271.43 μM0.29347817014829Chembridge (Drug-like library)384.816283.88415
SGTC_12971068-011114.1 μM0.291139767057ChemDiv (Drug-like library)312.214244.33712