st038117

ethyl 4-methyl-5-(pyrrolidine-1-carbonyl)-2-[(3,4,5-trimethoxybenzoyl)amino]thiophene-3-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1746
Screen concentration 42.0 μM
Source TimTec (Natural product derivative library)
PubChem CID 2898218
SMILES CCOC(=O)C1=C(SC(=C1C)C(=O)N2CCCC2)NC(=O)C3=CC(=C(C(=C3)OC)OC)OC
Standardized SMILES CCOC(=O)c1c(C)c(sc1NC(=O)c2cc(OC)c(OC)c(OC)c2)C(=O)N3CCCC3
Molecular weight 476.5426
ALogP 3.21
H-bond donor count 1
H-bond acceptor count 8
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) 1.07


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2898218
Download HIP data (tab-delimited text)  (excel)
Gene:APC4(YDR118W)|FD-Score:3.33|P-value:4.28E-4|Clearance:0.05||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress Gene:CAB2(YIL083C)|FD-Score:3.17|P-value:7.54E-4|Clearance:0.08||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:CDC16(YKL022C)|FD-Score:5.54|P-value:1.55E-8|Clearance:0.68||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:CDC53(YDL132W)|FD-Score:-3.68|P-value:1.18E-4|Clearance:0||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:CEP3(YMR168C)|FD-Score:4.04|P-value:2.72E-5|Clearance:0.32||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:GPI2(YPL076W)|FD-Score:-3.41|P-value:3.21E-4|Clearance:0||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein Gene:ISD11(YER048W-A)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.2||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:KEG1(YFR042W)|FD-Score:3.28|P-value:5.13E-4|Clearance:0.11||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:MMS21(YEL019C)|FD-Score:3.52|P-value:2.20E-4|Clearance:0.18||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:NUP1(YOR098C)|FD-Score:-3.09|P-value:9.85E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153 Gene:POB3(YML069W)|FD-Score:4.18|P-value:1.49E-5|Clearance:0.13||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress Gene:PRO3(YER023W)|FD-Score:4.86|P-value:5.86E-7|Clearance:0.68||SGD DESC:Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis Gene:RPO26(YPR187W)|FD-Score:-3.2|P-value:6.81E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RRB1(YMR131C)|FD-Score:7.21|P-value:2.78E-13|Clearance:1.68||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:SMC1(YFL008W)|FD-Score:4.04|P-value:2.63E-5|Clearance:0.01||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:TID3(YIL144W)|FD-Score:-3.22|P-value:6.48E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:APC4(YDR118W)|FD-Score:3.33|P-value:4.28E-4|Clearance:0.05||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress Gene:CAB2(YIL083C)|FD-Score:3.17|P-value:7.54E-4|Clearance:0.08||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:CDC16(YKL022C)|FD-Score:5.54|P-value:1.55E-8|Clearance:0.68||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:CDC53(YDL132W)|FD-Score:-3.68|P-value:1.18E-4|Clearance:0||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:CEP3(YMR168C)|FD-Score:4.04|P-value:2.72E-5|Clearance:0.32||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:GPI2(YPL076W)|FD-Score:-3.41|P-value:3.21E-4|Clearance:0||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein Gene:ISD11(YER048W-A)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.2||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:KEG1(YFR042W)|FD-Score:3.28|P-value:5.13E-4|Clearance:0.11||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:MMS21(YEL019C)|FD-Score:3.52|P-value:2.20E-4|Clearance:0.18||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:NUP1(YOR098C)|FD-Score:-3.09|P-value:9.85E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153 Gene:POB3(YML069W)|FD-Score:4.18|P-value:1.49E-5|Clearance:0.13||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress Gene:PRO3(YER023W)|FD-Score:4.86|P-value:5.86E-7|Clearance:0.68||SGD DESC:Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis Gene:RPO26(YPR187W)|FD-Score:-3.2|P-value:6.81E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RRB1(YMR131C)|FD-Score:7.21|P-value:2.78E-13|Clearance:1.68||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:SMC1(YFL008W)|FD-Score:4.04|P-value:2.63E-5|Clearance:0.01||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:TID3(YIL144W)|FD-Score:-3.22|P-value:6.48E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2898218
Download HOP data (tab-delimited text)  (excel)
Gene:AIM23(YJL131C)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss Gene:AST1(YBL069W)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATG7(YHR171W)|FD-Score:-5.09|P-value:1.75E-7||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:BEM1(YBR200W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p Gene:BIO3(YNR058W)|FD-Score:5.63|P-value:9.19E-9||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:COX12(YLR038C)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:COX15(YER141W)|FD-Score:7.23|P-value:2.41E-13||SGD DESC:Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase Gene:CRN1(YLR429W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly Gene:CRT10(YOL063C)|FD-Score:-3.22|P-value:6.48E-4||SGD DESC:Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat Gene:DAP2(YHR028C)|FD-Score:-8.88|P-value:3.36E-19||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DCN1(YLR128W)|FD-Score:4.53|P-value:2.95E-6||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:EBS1(YDR206W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors Gene:FCY22(YER060W-A)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FYV10(YIL097W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GLO2(YDR272W)|FD-Score:3.46|P-value:2.65E-4||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GLT1(YDL171C)|FD-Score:6.15|P-value:3.95E-10||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:GPB1(YOR371C)|FD-Score:5.3|P-value:5.81E-8||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:GPX2(YBR244W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress Gene:GRR1(YJR090C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GTR2(YGR163W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GUD1(YDL238C)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HOM2(YDR158W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HPM1(YIL110W)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:HTZ1(YOL012C)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:ICY1(YMR195W)|FD-Score:3.75|P-value:8.95E-5||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:INO4(YOL108C)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:IRC21(YMR073C)|FD-Score:9.14|P-value:3.09E-20||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LIN1(YHR156C)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MDM35(YKL053C-A)|FD-Score:5.14|P-value:1.34E-7||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MON1(YGL124C)|FD-Score:-4.32|P-value:7.87E-6||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MSS51(YLR203C)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:NIP100(YPL174C)|FD-Score:3.77|P-value:8.31E-5||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:OST5(YGL226C-A)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PEP7(YDR323C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX27(YOR193W)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PHO5(YBR093C)|FD-Score:5.36|P-value:4.20E-8||SGD DESC:Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 Gene:PHO87(YCR037C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PHO89(YBR296C)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:POL32(YJR043C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:POM34(YLR018C)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication Gene:PRR1(YKL116C)|FD-Score:-3.1|P-value:9.71E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:PUF3(YLL013C)|FD-Score:4.88|P-value:5.38E-7||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:PUS2(YGL063W)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:RAD50(YNL250W)|FD-Score:6.09|P-value:5.80E-10||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:REV3(YPL167C)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:ROM1(YGR070W)|FD-Score:5.21|P-value:9.53E-8||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPS14A(YCR031C)|FD-Score:3.79|P-value:7.44E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS23A(YGR118W)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:SAC7(YDR389W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAS3(YBL052C)|FD-Score:-4.36|P-value:6.46E-6||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SGM1(YJR134C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SIZ1(YDR409W)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SMA2(YML066C)|FD-Score:6.03|P-value:8.26E-10||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:SNA3(YJL151C)|FD-Score:-3.96|P-value:3.75E-5||SGD DESC:Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles Gene:SNC1(YAL030W)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SPC1(YJR010C-A)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SPH1(YLR313C)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:STB4(YMR019W)|FD-Score:-3.89|P-value:4.94E-5||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:SVP26(YHR181W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:SWD1(YAR003W)|FD-Score:6.91|P-value:2.35E-12||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:SWM2(YNR004W_p)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TGS1(YPL157W)|FD-Score:5.33|P-value:4.80E-8||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TOS2(YGR221C)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:UBA4(YHR111W)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UBX6(YJL048C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:VMA22(YHR060W)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VPS65(YLR322W_d)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YAL018C(YAL018C_p)|FD-Score:6.64|P-value:1.52E-11||SGD DESC:Putative protein of unknown function Gene:YAP3(YHL009C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YAR1(YPL239W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YBR099C(YBR099C_d)|FD-Score:5.72|P-value:5.21E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YBR200W-A(YBR200W-A_p)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCL002C(YCL002C_p)|FD-Score:-6.34|P-value:1.15E-10||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCL022C(YCL022C_d)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W Gene:YCL023C(YCL023C_d)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Gene:YCR007C(YCR007C_p)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDR161W(YDR161W_p)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR169C-A(YDR169C-A_p)|FD-Score:-5.89|P-value:1.95E-9||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR179W-A(YDR179W-A_p)|FD-Score:4.38|P-value:5.99E-6||SGD DESC:Putative protein of unknown function Gene:YDR379C-A(YDR379C-A)|FD-Score:-4.24|P-value:1.12E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YEL045C(YEL045C_d)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YGK3(YOL128C)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL159W(YGL159W_p)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGR290W(YGR290W_d)|FD-Score:-3.44|P-value:2.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YKR075C(YKR075C_p)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YLR241W(YLR241W_p)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YMR082C(YMR082C_d)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR1(YJR110W)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family Gene:YMR119W-A(YMR119W-A_d)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR178W(YMR178W_p)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; YMR178W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL095C(YNL095C_p)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication Gene:YOR318C(YOR318C_d)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YPL225W(YPL225W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR123C(YPR123C_d)|FD-Score:5.13|P-value:1.46E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPS1(YLR120C)|FD-Score:-4.79|P-value:8.40E-7||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YRB30(YGL164C)|FD-Score:4|P-value:3.17E-5||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes Gene:YVH1(YIR026C)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases Gene:AIM23(YJL131C)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss Gene:AST1(YBL069W)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATG7(YHR171W)|FD-Score:-5.09|P-value:1.75E-7||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:BEM1(YBR200W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p Gene:BIO3(YNR058W)|FD-Score:5.63|P-value:9.19E-9||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:COX12(YLR038C)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:COX15(YER141W)|FD-Score:7.23|P-value:2.41E-13||SGD DESC:Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase Gene:CRN1(YLR429W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly Gene:CRT10(YOL063C)|FD-Score:-3.22|P-value:6.48E-4||SGD DESC:Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat Gene:DAP2(YHR028C)|FD-Score:-8.88|P-value:3.36E-19||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DCN1(YLR128W)|FD-Score:4.53|P-value:2.95E-6||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:EBS1(YDR206W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors Gene:FCY22(YER060W-A)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FYV10(YIL097W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GLO2(YDR272W)|FD-Score:3.46|P-value:2.65E-4||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GLT1(YDL171C)|FD-Score:6.15|P-value:3.95E-10||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:GPB1(YOR371C)|FD-Score:5.3|P-value:5.81E-8||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:GPX2(YBR244W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress Gene:GRR1(YJR090C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GTR2(YGR163W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GUD1(YDL238C)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HOM2(YDR158W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HPM1(YIL110W)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:HTZ1(YOL012C)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:ICY1(YMR195W)|FD-Score:3.75|P-value:8.95E-5||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:INO4(YOL108C)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:IRC21(YMR073C)|FD-Score:9.14|P-value:3.09E-20||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LIN1(YHR156C)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MDM35(YKL053C-A)|FD-Score:5.14|P-value:1.34E-7||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MON1(YGL124C)|FD-Score:-4.32|P-value:7.87E-6||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MSS51(YLR203C)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:NIP100(YPL174C)|FD-Score:3.77|P-value:8.31E-5||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:OST5(YGL226C-A)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PEP7(YDR323C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX27(YOR193W)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PHO5(YBR093C)|FD-Score:5.36|P-value:4.20E-8||SGD DESC:Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 Gene:PHO87(YCR037C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PHO89(YBR296C)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:POL32(YJR043C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:POM34(YLR018C)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication Gene:PRR1(YKL116C)|FD-Score:-3.1|P-value:9.71E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:PUF3(YLL013C)|FD-Score:4.88|P-value:5.38E-7||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:PUS2(YGL063W)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:RAD50(YNL250W)|FD-Score:6.09|P-value:5.80E-10||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:REV3(YPL167C)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:ROM1(YGR070W)|FD-Score:5.21|P-value:9.53E-8||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPS14A(YCR031C)|FD-Score:3.79|P-value:7.44E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS23A(YGR118W)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:SAC7(YDR389W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAS3(YBL052C)|FD-Score:-4.36|P-value:6.46E-6||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SGM1(YJR134C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SIZ1(YDR409W)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SMA2(YML066C)|FD-Score:6.03|P-value:8.26E-10||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:SNA3(YJL151C)|FD-Score:-3.96|P-value:3.75E-5||SGD DESC:Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles Gene:SNC1(YAL030W)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SPC1(YJR010C-A)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SPH1(YLR313C)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:STB4(YMR019W)|FD-Score:-3.89|P-value:4.94E-5||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:SVP26(YHR181W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:SWD1(YAR003W)|FD-Score:6.91|P-value:2.35E-12||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:SWM2(YNR004W_p)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TGS1(YPL157W)|FD-Score:5.33|P-value:4.80E-8||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TOS2(YGR221C)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:UBA4(YHR111W)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UBX6(YJL048C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:VMA22(YHR060W)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VPS65(YLR322W_d)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YAL018C(YAL018C_p)|FD-Score:6.64|P-value:1.52E-11||SGD DESC:Putative protein of unknown function Gene:YAP3(YHL009C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YAR1(YPL239W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YBR099C(YBR099C_d)|FD-Score:5.72|P-value:5.21E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YBR200W-A(YBR200W-A_p)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCL002C(YCL002C_p)|FD-Score:-6.34|P-value:1.15E-10||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCL022C(YCL022C_d)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W Gene:YCL023C(YCL023C_d)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Gene:YCR007C(YCR007C_p)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDR161W(YDR161W_p)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR169C-A(YDR169C-A_p)|FD-Score:-5.89|P-value:1.95E-9||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR179W-A(YDR179W-A_p)|FD-Score:4.38|P-value:5.99E-6||SGD DESC:Putative protein of unknown function Gene:YDR379C-A(YDR379C-A)|FD-Score:-4.24|P-value:1.12E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YEL045C(YEL045C_d)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YGK3(YOL128C)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL159W(YGL159W_p)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGR290W(YGR290W_d)|FD-Score:-3.44|P-value:2.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YKR075C(YKR075C_p)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YLR241W(YLR241W_p)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YMR082C(YMR082C_d)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR1(YJR110W)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family Gene:YMR119W-A(YMR119W-A_d)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR178W(YMR178W_p)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; YMR178W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL095C(YNL095C_p)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication Gene:YOR318C(YOR318C_d)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YPL225W(YPL225W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR123C(YPR123C_d)|FD-Score:5.13|P-value:1.46E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPS1(YLR120C)|FD-Score:-4.79|P-value:8.40E-7||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YRB30(YGL164C)|FD-Score:4|P-value:3.17E-5||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes Gene:YVH1(YIR026C)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR131C7.212.78E-131.68RRB1Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p
YKL022C5.541.55E-80.68CDC16Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation
YER023W4.865.86E-70.69PRO3Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis
YML069W4.181.49E-50.13POB3Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress
YFL008W4.042.63E-50.01SMC1Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure
YMR168C4.042.72E-50.32CEP3Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
YER048W-A3.721.00E-40.20ISD11Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis
YEL019C3.522.20E-40.18MMS21SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination
YDR118W3.334.28E-40.05APC4Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
YFR042W3.285.13E-40.11KEG1Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability
YIL083C3.177.54E-40.08CAB2Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
YMR308C3.090.001000.07PSE1Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p
YCL043C3.020.001280.05PDI1Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
YGL061C2.970.001500.01DUO1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YJR023C_d2.960.001530.02YJR023C_dPutative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR073C9.143.09E-20IRC21Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study
YER141W7.232.41E-13COX15Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase
YAR003W6.912.35E-12SWD1Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7
YAL018C_p6.641.52E-11YAL018C_pPutative protein of unknown function
YDL171C6.153.95E-10GLT1NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source
YNL250W6.095.80E-10RAD50Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress
YML066C6.038.26E-10SMA2Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation
YBR099C_d5.725.21E-9YBR099C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
YNR058W5.639.19E-9BIO37,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis
YBR093C5.364.20E-8PHO5Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2
YPL157W5.334.80E-8TGS1Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
YOR371C5.305.81E-8GPB1Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication
YGR070W5.219.53E-8ROM1GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP
YKL053C-A5.141.34E-7MDM35Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
YPR123C_d5.131.46E-7YPR123C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR

GO enrichment analysis for SGTC_1746
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0655.39E-7SGTC_2507purpurin 79.7 μMMicrosource (Natural product library)66830.0540541copper-dependent oxidative stress
0.0621.88E-6SGTC_28669034245 58.4 μMChembridge (Drug-like library)64622730.129412
0.0622.17E-6SGTC_29349036370 36.9 μMChembridge (Drug-like library)64628000.159091
0.0603.50E-6SGTC_28799041815 71.4 μMChembridge (Drug-like library)49429710.151899
0.0595.63E-6SGTC_1997st073024 93.8 μMTimTec (Natural product derivative library)6862680.0365854
0.0596.20E-6SGTC_2671danthron 100.0 μMMicrosource (Natural product library)29500.0422535DNA damage response
0.0571.10E-5SGTC_2550chrysin dimethyl ether 71.9 μMMicrosource (Natural product library)888810.123457
0.0561.53E-5SGTC_1519esterone 74.0 μMTimTec (Pure natural product library)6980.0674157DNA intercalators
0.0534.15E-5SGTC_219mitoxanthrone 164.4 μMMiscellaneous42120.0625DNA intercalators
0.0519.98E-5SGTC_2555cholic acid 100.0 μMMicrosource (Natural product library)67086570.08
0.0501.10E-4SGTC_28619024595 19.5 μMChembridge (Drug-like library)164828100.25
0.0491.42E-4SGTC_264promethazine 12.5 mMMiscellaneous60140.0641026DNA intercalators
0.0491.65E-4SGTC_15034585-0012 61.3 μMChemDiv (Drug-like library)29220520.105263
0.0482.47E-4SGTC_1453737-0113 38.4 μMChemDiv (Drug-like library)68493510.0681818DNA intercalators
0.0482.47E-4SGTC_21715739187 198.1 μMChembridge (Fragment library)22627610.104478copper-dependent oxidative stress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_309k408-00511.48 μM0.3780493640463ChemDiv (Drug-like library)505.650043.32218
SGTC_2937904475311.33 μM0.3289476465530Chembridge (Drug-like library)393.543443.0921660S ribosome export
SGTC_1704st02880570.8 μM0.3285712876101TimTec (Natural product derivative library)282.358622.5724
SGTC_2913796655636.57 μM0.3205132976516Chembridge (Drug-like library)372.43813.3982560S ribosome export
SGTC_492tmb-8126 μM0.3142865494ICCB bioactive library395.532884.93906
SGTC_22537938677135.83 μM0.31252301972Chembridge (Fragment library)229.660181.66913
SGTC_3032908942749.47 μM0.30769217173707Chembridge (Drug-like library)318.390722.7291560S ribosome export
SGTC_9751335-004628.5 μM0.30263216486740ChemDiv (Drug-like library)374.250142.67915RPP1 & pyrimidine depletion
SGTC_14843473-2379286 μM0.30137671917ChemDiv (Drug-like library)279.311621.89825
SGTC_10134112-3890241 μM0.294872705171ChemDiv (Drug-like library)331.386183.39725TSC3-RPN4