st037496

9-benzyl-1,3,7-trimethylpurine-2,6,8-trione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1747
Screen concentration 66.6 μM
Source TimTec (Natural product derivative library)
PubChem CID 723305
SMILES CN1C2=C(N(C(=O)N(C2=O)C)C)N(C1=O)CC3=CC=CC=C3
Standardized SMILES CN1C(=O)N(C)C2=C(N(C)C(=O)N2Cc3ccccc3)C1=O
Molecular weight 300.3125
ALogP 0.64
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.18
% growth inhibition (Hom. pool) 7.14


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 723305
Download HIP data (tab-delimited text)  (excel)
Gene:CDC13(YDL220C)|FD-Score:7.39|P-value:7.14E-14|Clearance:1.92||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:CET1(YPL228W)|FD-Score:3.79|P-value:7.66E-5|Clearance:0.06||SGD DESC:Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:EMG1(YLR186W)|FD-Score:3.12|P-value:9.19E-4|Clearance:0.07||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:NAB3(YPL190C)|FD-Score:3.82|P-value:6.77E-5|Clearance:0.03||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:NHP2(YDL208W)|FD-Score:5.3|P-value:5.70E-8|Clearance:0.65||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:NIP1(YMR309C)|FD-Score:-3.91|P-value:4.57E-5|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOB1(YOR056C)|FD-Score:4.65|P-value:1.62E-6|Clearance:0.67||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:NOP53(YPL146C)|FD-Score:-3.93|P-value:4.26E-5|Clearance:0||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:NUP116(YMR047C)|FD-Score:-4.24|P-value:1.13E-5|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p) Gene:PRP9(YDL030W)|FD-Score:-4.43|P-value:4.72E-6|Clearance:0||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:PUP3(YER094C)|FD-Score:3.13|P-value:8.88E-4|Clearance:0||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RHO3(YIL118W)|FD-Score:3.96|P-value:3.79E-5|Clearance:0.08||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RPA43(YOR340C)|FD-Score:3.99|P-value:3.31E-5|Clearance:0.03||SGD DESC:RNA polymerase I subunit A43 Gene:RPC25(YKL144C)|FD-Score:3.63|P-value:1.44E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p Gene:RPM2(YML091C)|FD-Score:3.62|P-value:1.46E-4|Clearance:0.01||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RPT3(YDR394W)|FD-Score:3.27|P-value:5.44E-4|Clearance:0.01||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:SCM3(YDL139C)|FD-Score:3.23|P-value:6.15E-4|Clearance:0.11||SGD DESC:Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP Gene:SDO1(YLR022C)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes Gene:SHR3(YDL212W)|FD-Score:3.12|P-value:9.00E-4|Clearance:0.01||SGD DESC:Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface Gene:SMD1(YGR074W)|FD-Score:3.73|P-value:9.64E-5|Clearance:0.1||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SWC4(YGR002C)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.02||SGD DESC:Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex Gene:TBF1(YPL128C)|FD-Score:-3.94|P-value:4.05E-5|Clearance:0||SGD DESC:Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation Gene:TIF11(YMR260C)|FD-Score:5.47|P-value:2.25E-8|Clearance:0.17||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:UTP10(YJL109C)|FD-Score:-3.84|P-value:6.26E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance Gene:YDR413C(YDR413C_d)|FD-Score:-5.76|P-value:4.10E-9|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YML6(YML025C)|FD-Score:3.88|P-value:5.29E-5|Clearance:0.06||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins Gene:YPI1(YFR003C)|FD-Score:3.61|P-value:1.53E-4|Clearance:0.1||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance Gene:YPR177C(YPR177C_d)|FD-Score:3.52|P-value:2.20E-4|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex Gene:CDC13(YDL220C)|FD-Score:7.39|P-value:7.14E-14|Clearance:1.92||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:CET1(YPL228W)|FD-Score:3.79|P-value:7.66E-5|Clearance:0.06||SGD DESC:Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:EMG1(YLR186W)|FD-Score:3.12|P-value:9.19E-4|Clearance:0.07||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:NAB3(YPL190C)|FD-Score:3.82|P-value:6.77E-5|Clearance:0.03||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:NHP2(YDL208W)|FD-Score:5.3|P-value:5.70E-8|Clearance:0.65||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:NIP1(YMR309C)|FD-Score:-3.91|P-value:4.57E-5|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOB1(YOR056C)|FD-Score:4.65|P-value:1.62E-6|Clearance:0.67||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:NOP53(YPL146C)|FD-Score:-3.93|P-value:4.26E-5|Clearance:0||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:NUP116(YMR047C)|FD-Score:-4.24|P-value:1.13E-5|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p) Gene:PRP9(YDL030W)|FD-Score:-4.43|P-value:4.72E-6|Clearance:0||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:PUP3(YER094C)|FD-Score:3.13|P-value:8.88E-4|Clearance:0||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RHO3(YIL118W)|FD-Score:3.96|P-value:3.79E-5|Clearance:0.08||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RPA43(YOR340C)|FD-Score:3.99|P-value:3.31E-5|Clearance:0.03||SGD DESC:RNA polymerase I subunit A43 Gene:RPC25(YKL144C)|FD-Score:3.63|P-value:1.44E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p Gene:RPM2(YML091C)|FD-Score:3.62|P-value:1.46E-4|Clearance:0.01||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RPT3(YDR394W)|FD-Score:3.27|P-value:5.44E-4|Clearance:0.01||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:SCM3(YDL139C)|FD-Score:3.23|P-value:6.15E-4|Clearance:0.11||SGD DESC:Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP Gene:SDO1(YLR022C)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes Gene:SHR3(YDL212W)|FD-Score:3.12|P-value:9.00E-4|Clearance:0.01||SGD DESC:Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface Gene:SMD1(YGR074W)|FD-Score:3.73|P-value:9.64E-5|Clearance:0.1||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SWC4(YGR002C)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.02||SGD DESC:Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex Gene:TBF1(YPL128C)|FD-Score:-3.94|P-value:4.05E-5|Clearance:0||SGD DESC:Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation Gene:TIF11(YMR260C)|FD-Score:5.47|P-value:2.25E-8|Clearance:0.17||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:UTP10(YJL109C)|FD-Score:-3.84|P-value:6.26E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance Gene:YDR413C(YDR413C_d)|FD-Score:-5.76|P-value:4.10E-9|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YML6(YML025C)|FD-Score:3.88|P-value:5.29E-5|Clearance:0.06||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins Gene:YPI1(YFR003C)|FD-Score:3.61|P-value:1.53E-4|Clearance:0.1||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance Gene:YPR177C(YPR177C_d)|FD-Score:3.52|P-value:2.20E-4|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 723305
Download HOP data (tab-delimited text)  (excel)
Gene:AAD6(YFL056C)|FD-Score:-4.79|P-value:8.36E-7||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ADO1(YJR105W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:APL2(YKL135C)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:ARP8(YOR141C)|FD-Score:-4.35|P-value:6.83E-6||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ATG33(YLR356W)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes Gene:BSP1(YPR171W)|FD-Score:-5.06|P-value:2.12E-7||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:BUD6(YLR319C)|FD-Score:3.9|P-value:4.73E-5||SGD DESC:Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate Gene:BUD7(YOR299W)|FD-Score:-6.32|P-value:1.31E-10||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication Gene:CBP4(YGR174C)|FD-Score:-3.82|P-value:6.62E-5||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:CCP1(YKR066C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CKB2(YOR039W)|FD-Score:-5.72|P-value:5.47E-9||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CLB4(YLR210W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:CMR1(YDL156W)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS Gene:CRF1(YDR223W)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain Gene:CWC15(YDR163W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p Gene:DCI1(YOR180C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DFG5(YMR238W)|FD-Score:6.29|P-value:1.64E-10||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DFM1(YDR411C)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p Gene:DNF3(YMR162C)|FD-Score:-4.67|P-value:1.53E-6||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase Gene:EBS1(YDR206W)|FD-Score:9.23|P-value:1.35E-20||SGD DESC:Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors Gene:ECM32(YER176W)|FD-Score:4.31|P-value:8.08E-6||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:EST1(YLR233C)|FD-Score:-4.44|P-value:4.51E-6||SGD DESC:TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA Gene:FLC1(YPL221W)|FD-Score:-3.9|P-value:4.89E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance Gene:FSF1(YOR271C_p)|FD-Score:-3.47|P-value:2.63E-4||SGD DESC:Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis Gene:GEA1(YJR031C)|FD-Score:-5.4|P-value:3.27E-8||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GIS2(YNL255C)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GMC1(YDR506C)|FD-Score:-3.85|P-value:5.82E-5||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:GUP1(YGL084C)|FD-Score:-4.89|P-value:5.07E-7||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HSE1(YHL002W)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:HSP26(YBR072W)|FD-Score:-3.25|P-value:5.71E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:HSP42(YDR171W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:IRS4(YKR019C)|FD-Score:4.78|P-value:8.82E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KES1(YPL145C)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:KTI12(YKL110C)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:LTE1(YAL024C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAE1(YKL029C)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MDH1(YKL085W)|FD-Score:4.32|P-value:7.74E-6||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:MMT2(YPL224C)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MTC1(YJL123C)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 Gene:NAM7(YMR080C)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:NMD2(YHR077C)|FD-Score:-4.44|P-value:4.50E-6||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NNT1(YLR285W)|FD-Score:4.87|P-value:5.67E-7||SGD DESC:S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination Gene:NOP16(YER002W)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:NPR1(YNL183C)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:NUP120(YKL057C)|FD-Score:5.51|P-value:1.81E-8||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OPI9(YLR338W_d)|FD-Score:-3.13|P-value:8.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OSH6(YKR003W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PDR3(YBL005W)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:PEP3(YLR148W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX11(YOL147C)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p Gene:PGM1(YKL127W)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism Gene:PIR1(YKL164C)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:PSY4(YBL046W)|FD-Score:-5.78|P-value:3.73E-9||SGD DESC:Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 Gene:REV1(YOR346W)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RGC1(YPR115W)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RPL14A(YKL006W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-3.79|P-value:7.67E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS18B(YML026C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSM25(YIL093C)|FD-Score:-5.51|P-value:1.81E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SAN1(YDR143C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SNA3(YJL151C)|FD-Score:-3.97|P-value:3.65E-5||SGD DESC:Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles Gene:SNF4(YGL115W)|FD-Score:-4.9|P-value:4.74E-7||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SNT1(YCR033W)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance Gene:STI1(YOR027W)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:SWD1(YAR003W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:SYO1(YDL063C)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TGL3(YMR313C)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TOS8(YGL096W)|FD-Score:-3.3|P-value:4.83E-4||SGD DESC:Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication Gene:TRM2(YKR056W)|FD-Score:-4.02|P-value:2.89E-5||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:TRM82(YDR165W)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TUF1(YOR187W)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:TYE7(YOR344C)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression Gene:UBC7(YMR022W)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:URE2(YNL229C)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VAC7(YNL054W)|FD-Score:-5.18|P-value:1.11E-7||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VPS61(YDR136C_d)|FD-Score:4.3|P-value:8.63E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBL065W(YBL065W_d)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene Gene:YBR197C(YBR197C_p)|FD-Score:-3.14|P-value:8.52E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YBR284W(YBR284W_p)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCH1(YGR203W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YCK2(YNL154C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking; YCK2 has a paralog, YCK1, that arose from the whole genome duplication Gene:YCL046W(YCL046W_d)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YCR049C(YCR049C_d)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL086W(YDL086W_p)|FD-Score:-3.73|P-value:9.52E-5||SGD DESC:Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene Gene:YDR203W(YDR203W_d)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR426C(YDR426C_d)|FD-Score:-3.99|P-value:3.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 Gene:YGR210C(YGR210C_p)|FD-Score:3.79|P-value:7.51E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR266W(YGR266W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YJL218W(YJL218W_p)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YLR030W(YLR030W_p)|FD-Score:-4.77|P-value:9.14E-7||SGD DESC:Putative protein of unknown function Gene:YLR179C(YLR179C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus Gene:YMR119W-A(YMR119W-A_d)|FD-Score:18.6|P-value:8.90E-78||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR175W-A(YMR175W-A_p)|FD-Score:-5.83|P-value:2.78E-9||SGD DESC:Putative protein of unknown function Gene:YNL108C(YNL108C_p)|FD-Score:5.4|P-value:3.35E-8||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YNL200C(YNL200C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNR021W(YNR021W_p)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene Gene:YOL164W-A(YOL164W-A_p)|FD-Score:-3.42|P-value:3.18E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YOR055W(YOR055W_d)|FD-Score:-4.11|P-value:2.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR105W(YOR105W_p)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR338W(YOR338W_p)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication Gene:YPS1(YLR120C)|FD-Score:5.52|P-value:1.68E-8||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YPT53(YNL093W)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:AAD6(YFL056C)|FD-Score:-4.79|P-value:8.36E-7||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ADO1(YJR105W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:APL2(YKL135C)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:ARP8(YOR141C)|FD-Score:-4.35|P-value:6.83E-6||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ATG33(YLR356W)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes Gene:BSP1(YPR171W)|FD-Score:-5.06|P-value:2.12E-7||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:BUD6(YLR319C)|FD-Score:3.9|P-value:4.73E-5||SGD DESC:Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate Gene:BUD7(YOR299W)|FD-Score:-6.32|P-value:1.31E-10||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication Gene:CBP4(YGR174C)|FD-Score:-3.82|P-value:6.62E-5||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:CCP1(YKR066C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CKB2(YOR039W)|FD-Score:-5.72|P-value:5.47E-9||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CLB4(YLR210W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:CMR1(YDL156W)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS Gene:CRF1(YDR223W)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain Gene:CWC15(YDR163W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p Gene:DCI1(YOR180C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DFG5(YMR238W)|FD-Score:6.29|P-value:1.64E-10||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DFM1(YDR411C)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p Gene:DNF3(YMR162C)|FD-Score:-4.67|P-value:1.53E-6||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase Gene:EBS1(YDR206W)|FD-Score:9.23|P-value:1.35E-20||SGD DESC:Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors Gene:ECM32(YER176W)|FD-Score:4.31|P-value:8.08E-6||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:EST1(YLR233C)|FD-Score:-4.44|P-value:4.51E-6||SGD DESC:TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA Gene:FLC1(YPL221W)|FD-Score:-3.9|P-value:4.89E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance Gene:FSF1(YOR271C_p)|FD-Score:-3.47|P-value:2.63E-4||SGD DESC:Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis Gene:GEA1(YJR031C)|FD-Score:-5.4|P-value:3.27E-8||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GIS2(YNL255C)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GMC1(YDR506C)|FD-Score:-3.85|P-value:5.82E-5||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:GUP1(YGL084C)|FD-Score:-4.89|P-value:5.07E-7||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HSE1(YHL002W)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:HSP26(YBR072W)|FD-Score:-3.25|P-value:5.71E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:HSP42(YDR171W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:IRS4(YKR019C)|FD-Score:4.78|P-value:8.82E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KES1(YPL145C)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:KTI12(YKL110C)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:LTE1(YAL024C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAE1(YKL029C)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MDH1(YKL085W)|FD-Score:4.32|P-value:7.74E-6||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:MMT2(YPL224C)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MTC1(YJL123C)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 Gene:NAM7(YMR080C)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:NMD2(YHR077C)|FD-Score:-4.44|P-value:4.50E-6||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NNT1(YLR285W)|FD-Score:4.87|P-value:5.67E-7||SGD DESC:S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination Gene:NOP16(YER002W)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:NPR1(YNL183C)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:NUP120(YKL057C)|FD-Score:5.51|P-value:1.81E-8||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OPI9(YLR338W_d)|FD-Score:-3.13|P-value:8.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OSH6(YKR003W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PDR3(YBL005W)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:PEP3(YLR148W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX11(YOL147C)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p Gene:PGM1(YKL127W)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism Gene:PIR1(YKL164C)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:PSY4(YBL046W)|FD-Score:-5.78|P-value:3.73E-9||SGD DESC:Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 Gene:REV1(YOR346W)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RGC1(YPR115W)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RPL14A(YKL006W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-3.79|P-value:7.67E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS18B(YML026C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSM25(YIL093C)|FD-Score:-5.51|P-value:1.81E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SAN1(YDR143C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SNA3(YJL151C)|FD-Score:-3.97|P-value:3.65E-5||SGD DESC:Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles Gene:SNF4(YGL115W)|FD-Score:-4.9|P-value:4.74E-7||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SNT1(YCR033W)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance Gene:STI1(YOR027W)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:SWD1(YAR003W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:SYO1(YDL063C)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TGL3(YMR313C)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TOS8(YGL096W)|FD-Score:-3.3|P-value:4.83E-4||SGD DESC:Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication Gene:TRM2(YKR056W)|FD-Score:-4.02|P-value:2.89E-5||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:TRM82(YDR165W)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TUF1(YOR187W)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:TYE7(YOR344C)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression Gene:UBC7(YMR022W)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:URE2(YNL229C)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VAC7(YNL054W)|FD-Score:-5.18|P-value:1.11E-7||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VPS61(YDR136C_d)|FD-Score:4.3|P-value:8.63E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBL065W(YBL065W_d)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene Gene:YBR197C(YBR197C_p)|FD-Score:-3.14|P-value:8.52E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YBR284W(YBR284W_p)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCH1(YGR203W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YCK2(YNL154C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking; YCK2 has a paralog, YCK1, that arose from the whole genome duplication Gene:YCL046W(YCL046W_d)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YCR049C(YCR049C_d)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL086W(YDL086W_p)|FD-Score:-3.73|P-value:9.52E-5||SGD DESC:Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene Gene:YDR203W(YDR203W_d)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR426C(YDR426C_d)|FD-Score:-3.99|P-value:3.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 Gene:YGR210C(YGR210C_p)|FD-Score:3.79|P-value:7.51E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR266W(YGR266W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YJL218W(YJL218W_p)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YLR030W(YLR030W_p)|FD-Score:-4.77|P-value:9.14E-7||SGD DESC:Putative protein of unknown function Gene:YLR179C(YLR179C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus Gene:YMR119W-A(YMR119W-A_d)|FD-Score:18.6|P-value:8.90E-78||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR175W-A(YMR175W-A_p)|FD-Score:-5.83|P-value:2.78E-9||SGD DESC:Putative protein of unknown function Gene:YNL108C(YNL108C_p)|FD-Score:5.4|P-value:3.35E-8||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YNL200C(YNL200C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNR021W(YNR021W_p)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene Gene:YOL164W-A(YOL164W-A_p)|FD-Score:-3.42|P-value:3.18E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YOR055W(YOR055W_d)|FD-Score:-4.11|P-value:2.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR105W(YOR105W_p)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR338W(YOR338W_p)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication Gene:YPS1(YLR120C)|FD-Score:5.52|P-value:1.68E-8||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YPT53(YNL093W)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL220C7.397.14E-141.92CDC13Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation
YMR260C5.472.25E-80.17TIF11Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3
YDL208W5.305.70E-80.65NHP2Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing
YOR056C4.651.62E-60.67NOB1Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress
YOR340C3.993.31E-50.03RPA43RNA polymerase I subunit A43
YIL118W3.963.79E-50.08RHO3Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p
YML025C3.885.29E-50.06YML6Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
YPL190C3.826.77E-50.03NAB3RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing
YPL228W3.797.66E-50.06CET1Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
YGR074W3.739.64E-50.10SMD1Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress
YKL144C3.631.44E-40.00RPC25RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p
YML091C3.621.46E-40.01RPM2Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus
YFR003C3.611.53E-40.10YPI1Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance
YPR177C_d3.522.20E-40.25YPR177C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex
YDR394W3.275.44E-40.01RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR119W-A_d18.608.90E-78YMR119W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR206W9.231.35E-20EBS1Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors
YMR238W6.291.64E-10DFG5Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p
YLR120C5.521.68E-8YPS1Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
YKL057C5.511.81E-8NUP120Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160
YNL108C_p5.403.35E-8YNL108C_pPutative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication
YLR285W4.875.67E-7NNT1S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination
YKR019C4.788.82E-7IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YOR180C4.701.28E-6DCI1Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed
YKL085W4.327.74E-6MDH1Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated
YER176W4.318.08E-6ECM32DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes
YDR136C_d4.308.63E-6VPS61_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect
YOR187W4.251.06E-5TUF1Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans
YBL065W_d4.221.24E-5YBL065W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene
YNL154C4.111.98E-5YCK2Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking; YCK2 has a paralog, YCK1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1747
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0781.86E-9SGTC_1740st038286 13.2 μMTimTec (Natural product derivative library)7167860.214286
0.0603.39E-6SGTC_1050111-0055 734.8 μMChemDiv (Drug-like library)51793700.0185185
0.0604.33E-6SGTC_2762leflunomide 42.1 μMMiscellaneous38990.0793651
0.0571.07E-5SGTC_29699091408 28.7 μMChembridge (Drug-like library)42863110.129032
0.0571.28E-5SGTC_9701315-0089 19.5 μMChemDiv (Drug-like library)68110830.0933333RSC & ERG11
0.0561.42E-5SGTC_7764476-4378 112.0 μMChemDiv (Drug-like library)28920080.0821918ERG2
0.0525.90E-5SGTC_12780874-0585 172.0 μMChemDiv (Drug-like library)7663060.111111
0.0526.36E-5SGTC_2811672-3440 14.9 μMChemDiv (Drug-like library)68330000.0547945Golgi
0.0518.00E-5SGTC_30709117005 49.5 μMChembridge (Drug-like library)87790160.0952381
0.0518.46E-5SGTC_12720839-0021 27.0 μMChemDiv (Drug-like library)50272500.12963
0.0519.78E-5SGTC_1235benzylparaben 21.5 μMChemDiv (Drug-like library)71800.2
0.0501.21E-4SGTC_33019125354 19.8 μMChembridge (Drug-like library)419051620.0869565
0.0501.29E-4SGTC_415dibenzyl ketone 100.0 μMMiscellaneous75930.25641
0.0491.90E-4SGTC_2720fluspirilene 36.9 μMMiscellaneous33960.140845ERG2
0.0491.90E-4SGTC_29629082311 13.2 μMChembridge (Drug-like library)164594530.136364

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_13161272-005065.9 μM0.3617027340392ChemDiv (Drug-like library)251.283222.02213
SGTC_1742st03823528.89 μM0.355932564103TimTec (Natural product derivative library)380.827583.73103mitochondrial stress
SGTC_1733st03735754.9 μM0.3389831272339TimTec (Natural product derivative library)364.3729833.2720460S ribosome export
SGTC_7864544-0069423 μM0.333333748821ChemDiv (Drug-like library)268.310442.73602
SGTC_14163966-032125.3 μM0.333333582530ChemDiv (Drug-like library)252.267981.8312
SGTC_1734st03716869.4 μM0.333333708907TimTec (Natural product derivative library)288.2770231.68904
SGTC_544k015-002052.4 μM0.31253008304ChemDiv (Drug-like library)316.14943.20802
SGTC_23427990637118.86 μM0.3076922033102Chembridge (Fragment library)242.23341.07123RNA processing & uracil transport
SGTC_2007401153971.82 μM0.30612245595301Chembridge (Fragment library)285.180181.42712cell wall signaling
SGTC_1748st03745553.6 μM0.298246580690TimTec (Natural product derivative library)339.176683.02403