st043925

3,4-dihydro-1H-isoquinolin-2-yl-(2-methoxy-4-methylsulfanylphenyl)methanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1753
Screen concentration 64.0 μM
Source TimTec (Natural product derivative library)
PubChem CID 670181
SMILES COC1=C(C=CC(=C1)SC)C(=O)N2CCC3=CC=CC=C3C2
Standardized SMILES COc1cc(SC)ccc1C(=O)N2CCc3ccccc3C2
Molecular weight 313.414
ALogP 3.43
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 18.13
% growth inhibition (Hom. pool) 11.78


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 670181
Download HIP data (tab-delimited text)  (excel)
Gene:DBP10(YDL031W)|FD-Score:4.01|P-value:3.02E-5|Clearance:0.54||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis Gene:DED81(YHR019C)|FD-Score:3.18|P-value:7.28E-4|Clearance:0||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:ERD2(YBL040C)|FD-Score:3.1|P-value:9.66E-4|Clearance:0.09||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:RPP1(YHR062C)|FD-Score:3.18|P-value:7.39E-4|Clearance:0.08||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP12(YPL012W)|FD-Score:3.27|P-value:5.40E-4|Clearance:0.03||SGD DESC:Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats Gene:RSC3(YDR303C)|FD-Score:-4.11|P-value:2.00E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p Gene:SMD3(YLR147C)|FD-Score:3.24|P-value:5.95E-4|Clearance:0.06||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SNU114(YKL173W)|FD-Score:4.81|P-value:7.73E-7|Clearance:0.79||SGD DESC:GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 Gene:SPC19(YDR201W)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.06||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:STT4(YLR305C)|FD-Score:3.47|P-value:2.61E-4|Clearance:0.01||SGD DESC:Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization Gene:YCL041C(YCL041C_d)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W Gene:YOR203W(YOR203W_d)|FD-Score:4.02|P-value:2.91E-5|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:DBP10(YDL031W)|FD-Score:4.01|P-value:3.02E-5|Clearance:0.54||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis Gene:DED81(YHR019C)|FD-Score:3.18|P-value:7.28E-4|Clearance:0||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:ERD2(YBL040C)|FD-Score:3.1|P-value:9.66E-4|Clearance:0.09||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:RPP1(YHR062C)|FD-Score:3.18|P-value:7.39E-4|Clearance:0.08||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP12(YPL012W)|FD-Score:3.27|P-value:5.40E-4|Clearance:0.03||SGD DESC:Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats Gene:RSC3(YDR303C)|FD-Score:-4.11|P-value:2.00E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p Gene:SMD3(YLR147C)|FD-Score:3.24|P-value:5.95E-4|Clearance:0.06||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SNU114(YKL173W)|FD-Score:4.81|P-value:7.73E-7|Clearance:0.79||SGD DESC:GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 Gene:SPC19(YDR201W)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.06||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:STT4(YLR305C)|FD-Score:3.47|P-value:2.61E-4|Clearance:0.01||SGD DESC:Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization Gene:YCL041C(YCL041C_d)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W Gene:YOR203W(YOR203W_d)|FD-Score:4.02|P-value:2.91E-5|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 670181
Download HOP data (tab-delimited text)  (excel)
Gene:AIM9(YER080W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARG5,6(YER069W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:BTS1(YPL069C)|FD-Score:-5.41|P-value:3.12E-8||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CSH1(YBR161W)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:EAF5(YEL018W)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:EBS1(YDR206W)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors Gene:ERV2(YPR037C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER Gene:FMP27(YLR454W_p)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GSY1(YFR015C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GTS1(YGL181W)|FD-Score:4.37|P-value:6.24E-6||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:HBN1(YCL026C-B_p)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HMX1(YLR205C)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants Gene:IRC13(YOR235W_d)|FD-Score:-6.14|P-value:4.09E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:ITR1(YDR497C)|FD-Score:5.2|P-value:9.70E-8||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:KNH1(YDL049C)|FD-Score:5.08|P-value:1.93E-7||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:LSC2(YGR244C)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:OMS1(YDR316W)|FD-Score:4.27|P-value:9.78E-6||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PHO91(YNR013C)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth Gene:PKP2(YGL059W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:POR2(YIL114C)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPS21A(YKR057W)|FD-Score:-4.69|P-value:1.37E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication Gene:SGA1(YIL099W)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation Gene:TAE1(YBR261C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm Gene:TMA108(YIL137C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TRK2(YKR050W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication Gene:TVP15(YDR100W)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p Gene:UBC8(YEL012W)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:UFD4(YKL010C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:UGX2(YDL169C)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:UTP30(YKR060W)|FD-Score:-3.1|P-value:9.75E-4||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VID28(YIL017C)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VPS9(YML097C)|FD-Score:-3.78|P-value:7.82E-5||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YDR015C(YDR015C_d)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YDR095C(YDR095C_d)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL165C(YGL165C_d)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W Gene:YGL242C(YGL242C_p)|FD-Score:5.81|P-value:3.11E-9||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YGR021W(YGR021W_p)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YGR045C(YGR045C_d)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL136W(YKL136W_d)|FD-Score:3.82|P-value:6.58E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YKL202W(YKL202W_d)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR419W(YLR419W_p)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YMR247W-A(YMR247W-A_p)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Putative protein of unknown function Gene:YNL035C(YNL035C_p)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPR003C(YPR003C_p)|FD-Score:-3.17|P-value:7.58E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR117W(YPR117W_p)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Putative protein of unknown function Gene:AIM9(YER080W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARG5,6(YER069W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:BTS1(YPL069C)|FD-Score:-5.41|P-value:3.12E-8||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CSH1(YBR161W)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:EAF5(YEL018W)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:EBS1(YDR206W)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors Gene:ERV2(YPR037C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER Gene:FMP27(YLR454W_p)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GSY1(YFR015C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GTS1(YGL181W)|FD-Score:4.37|P-value:6.24E-6||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:HBN1(YCL026C-B_p)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HMX1(YLR205C)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants Gene:IRC13(YOR235W_d)|FD-Score:-6.14|P-value:4.09E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:ITR1(YDR497C)|FD-Score:5.2|P-value:9.70E-8||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:KNH1(YDL049C)|FD-Score:5.08|P-value:1.93E-7||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:LSC2(YGR244C)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:OMS1(YDR316W)|FD-Score:4.27|P-value:9.78E-6||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PHO91(YNR013C)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth Gene:PKP2(YGL059W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:POR2(YIL114C)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPS21A(YKR057W)|FD-Score:-4.69|P-value:1.37E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication Gene:SGA1(YIL099W)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation Gene:TAE1(YBR261C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm Gene:TMA108(YIL137C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TRK2(YKR050W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication Gene:TVP15(YDR100W)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p Gene:UBC8(YEL012W)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:UFD4(YKL010C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:UGX2(YDL169C)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:UTP30(YKR060W)|FD-Score:-3.1|P-value:9.75E-4||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VID28(YIL017C)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VPS9(YML097C)|FD-Score:-3.78|P-value:7.82E-5||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YDR015C(YDR015C_d)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YDR095C(YDR095C_d)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL165C(YGL165C_d)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W Gene:YGL242C(YGL242C_p)|FD-Score:5.81|P-value:3.11E-9||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YGR021W(YGR021W_p)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YGR045C(YGR045C_d)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL136W(YKL136W_d)|FD-Score:3.82|P-value:6.58E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YKL202W(YKL202W_d)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR419W(YLR419W_p)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YMR247W-A(YMR247W-A_p)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Putative protein of unknown function Gene:YNL035C(YNL035C_p)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPR003C(YPR003C_p)|FD-Score:-3.17|P-value:7.58E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR117W(YPR117W_p)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL173W4.817.73E-70.79SNU114GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2
YOR203W_d4.022.91E-50.01YOR203W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation
YDL031W4.013.02E-50.54DBP10Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis
YLR305C3.472.61E-40.01STT4Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization
YCL041C_d3.462.72E-40.13YCL041C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W
YDR201W3.334.40E-40.06SPC19Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
YPL012W3.275.40E-40.03RRP12Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats
YLR147C3.245.95E-40.06SMD3Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3
YHR019C3.187.28E-40.00DED81Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA
YHR062C3.187.39E-40.08RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YBL040C3.109.66E-40.09ERD2HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins
YBR152W3.010.001320.05SPP381mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p
YLR310C2.960.001550.01CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YDL008W2.940.001620.13APC11Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity
YOL097C2.820.002420.07WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL242C_p5.813.11E-9YGL242C_pPutative protein of unknown function; deletion mutant is viable
YDR497C5.209.70E-8ITR1Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress
YDL049C5.081.93E-7KNH1Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
YGL181W4.376.24E-6GTS1Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations
YDR316W4.279.78E-6OMS1Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations
YNR013C4.171.55E-5PHO91Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth
YGL165C_d4.072.38E-5YGL165C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W
YLR454W_p3.983.41E-5FMP27_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YLR419W_p3.924.34E-5YLR419W_pPutative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene
YKL136W_d3.826.58E-5YKL136W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C
YKL202W_d3.797.57E-5YKL202W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YKR050W3.631.43E-4TRK2Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication
YKL010C3.591.63E-4UFD4Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress
YBR261C3.532.09E-4TAE1AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm
YER069W3.532.11E-4ARG5,6Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine

GO enrichment analysis for SGTC_1753
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1453.29E-29SGTC_1756st041558 35.5 μMTimTec (Natural product derivative library)39557920.132653
0.0992.55E-14SGTC_14123909-8563 178.0 μMChemDiv (Drug-like library)237675300.117021
0.0841.21E-10SGTC_14394092-1168 46.0 μMChemDiv (Drug-like library)28862400.166667
0.0822.21E-10SGTC_2419paclitaxel 166.5 μMMiscellaneous46660.092437
0.0722.94E-8SGTC_2414st077232 92.9 μMTimTec (Natural product derivative library)7298010.134328
0.0691.26E-7SGTC_31609100216 49.5 μMChembridge (Drug-like library)171910390.210526
0.0649.06E-7SGTC_1777st045864 34.8 μMTimTec (Natural product derivative library)46914620.0875
0.0631.07E-6SGTC_2744captopril 92.0 μMMiscellaneous440930.10294160S ribosome export
0.0612.95E-6SGTC_32469135460 33.0 μMChembridge (Drug-like library)414469510.152778
0.0594.98E-6SGTC_521r062-0005 13.8 μMChemDiv (Drug-like library)28320330.119403
0.0597.11E-6SGTC_30199081971 49.5 μMChembridge (Drug-like library)164518030.1
0.0588.52E-6SGTC_2545solidagenone 89.6 μMMicrosource (Natural product library)67085720.0714286
0.0571.09E-5SGTC_820054-0107 293.8 μMChemDiv (Drug-like library)31046970.112676
0.0552.11E-5SGTC_465cgp-37157 41.0 μMICCB bioactive library26880.109589
0.0543.10E-5SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.113924RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1770st04388466.3 μM0.615385670149TimTec (Natural product derivative library)301.767443.5502
SGTC_1653st01211265 μM0.5720429TimTec (Natural product derivative library)281.348962.9202
SGTC_1835st00866649.2 μM0.490566690455TimTec (Natural product derivative library)306.186524.2301
SGTC_1636st00770768.9 μM0.3125403051TimTec (Natural product derivative library)290.359023.75911
SGTC_10204335-096193.3 μM0.306452669075ChemDiv (Drug-like library)267.322382.91302
SGTC_11584092-049491.8 μM0.2985071148199ChemDiv (Drug-like library)416.512124.2704
SGTC_3177910750649.47 μM0.2985078897194Chembridge (Drug-like library)314.809263.76512ERG2
SGTC_3178910750649.47 μM0.2985078897194Chembridge (Drug-like library)314.809263.76512
SGTC_1531st05929752.4 μM0.2957757566702TimTec (Pure natural product library)457.517685.22206
SGTC_3113912349849.47 μM0.2923088897275Chembridge (Drug-like library)308.417363.80812
SGTC_14464239-019062 μM0.2876711340085ChemDiv (Drug-like library)379.495263.87414