st041960

6-[2-(4-methyl-2-propan-2-ylphenoxy)ethylsulfanyl]-7H-purine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1755
Screen concentration 45.2 μM
Source TimTec (Natural product derivative library)
PubChem CID 2257073
SMILES CC1=CC(=C(C=C1)OCCSC2=NC=NC3=C2NC=N3)C(C)C
Standardized SMILES CC(C)c1cc(C)ccc1OCCSc2[nH]cnc3ncnc23
Molecular weight 328.4319
ALogP 4.16
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 18.28
% growth inhibition (Hom. pool) 11.27


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2257073
Download HIP data (tab-delimited text)  (excel)
Gene:BRR6(YGL247W)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.11||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:CCT3(YJL014W)|FD-Score:3.39|P-value:3.44E-4|Clearance:0.03||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:3.99|P-value:3.25E-5|Clearance:0.27||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC21(YOR074C)|FD-Score:4.7|P-value:1.31E-6|Clearance:0.2||SGD DESC:Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S Gene:DBP5(YOR046C)|FD-Score:3.72|P-value:9.93E-5|Clearance:0.2||SGD DESC:Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p Gene:ERG12(YMR208W)|FD-Score:-4.62|P-value:1.88E-6|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG7(YHR072W)|FD-Score:5.47|P-value:2.21E-8|Clearance:0.32||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:FIP1(YJR093C)|FD-Score:3.25|P-value:5.69E-4|Clearance:0.17||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:LSG1(YGL099W)|FD-Score:6.78|P-value:6.12E-12|Clearance:1.3||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MCM10(YIL150C)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.02||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MEC1(YBR136W)|FD-Score:4.72|P-value:1.18E-6|Clearance:0.02||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:MYO1(YHR023W)|FD-Score:4.9|P-value:4.73E-7|Clearance:0.18||SGD DESC:Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively Gene:NMD3(YHR170W)|FD-Score:5.15|P-value:1.28E-7|Clearance:0.25||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:OLE1(YGL055W)|FD-Score:4.5|P-value:3.39E-6|Clearance:0.39||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PMI40(YER003C)|FD-Score:-3.14|P-value:8.49E-4|Clearance:0||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:RPC19(YNL113W)|FD-Score:3.36|P-value:3.87E-4|Clearance:0.11||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RRP45(YDR280W)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.12||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SPP381(YBR152W)|FD-Score:-3.2|P-value:6.87E-4|Clearance:0||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:STS1(YIR011C)|FD-Score:-3.13|P-value:8.64E-4|Clearance:0||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:UTR5(YEL035C_p)|FD-Score:-3.09|P-value:9.99E-4|Clearance:0||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:BRR6(YGL247W)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.11||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:CCT3(YJL014W)|FD-Score:3.39|P-value:3.44E-4|Clearance:0.03||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:3.99|P-value:3.25E-5|Clearance:0.27||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC21(YOR074C)|FD-Score:4.7|P-value:1.31E-6|Clearance:0.2||SGD DESC:Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S Gene:DBP5(YOR046C)|FD-Score:3.72|P-value:9.93E-5|Clearance:0.2||SGD DESC:Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p Gene:ERG12(YMR208W)|FD-Score:-4.62|P-value:1.88E-6|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG7(YHR072W)|FD-Score:5.47|P-value:2.21E-8|Clearance:0.32||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:FIP1(YJR093C)|FD-Score:3.25|P-value:5.69E-4|Clearance:0.17||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:LSG1(YGL099W)|FD-Score:6.78|P-value:6.12E-12|Clearance:1.3||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MCM10(YIL150C)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.02||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MEC1(YBR136W)|FD-Score:4.72|P-value:1.18E-6|Clearance:0.02||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:MYO1(YHR023W)|FD-Score:4.9|P-value:4.73E-7|Clearance:0.18||SGD DESC:Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively Gene:NMD3(YHR170W)|FD-Score:5.15|P-value:1.28E-7|Clearance:0.25||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:OLE1(YGL055W)|FD-Score:4.5|P-value:3.39E-6|Clearance:0.39||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PMI40(YER003C)|FD-Score:-3.14|P-value:8.49E-4|Clearance:0||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:RPC19(YNL113W)|FD-Score:3.36|P-value:3.87E-4|Clearance:0.11||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RRP45(YDR280W)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.12||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SPP381(YBR152W)|FD-Score:-3.2|P-value:6.87E-4|Clearance:0||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:STS1(YIR011C)|FD-Score:-3.13|P-value:8.64E-4|Clearance:0||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:UTR5(YEL035C_p)|FD-Score:-3.09|P-value:9.99E-4|Clearance:0||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2257073
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:5.98|P-value:1.13E-9||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADY2(YCR010C)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:ANS1(YHR126C_p)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:APS1(YLR170C)|FD-Score:-5.13|P-value:1.43E-7||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ATG22(YCL038C)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:BFR1(YOR198C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BRE5(YNR051C)|FD-Score:5.28|P-value:6.64E-8||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CDC26(YFR036W)|FD-Score:10.1|P-value:3.07E-24||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CIT1(YNR001C)|FD-Score:4.08|P-value:2.30E-5||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:DUG3(YNL191W)|FD-Score:-4.21|P-value:1.30E-5||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EMP70(YLR083C)|FD-Score:-5.57|P-value:1.29E-8||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:ENV7(YPL236C)|FD-Score:-4.12|P-value:1.91E-5||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:EOS1(YNL080C)|FD-Score:4.31|P-value:8.25E-6||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:ERV41(YML067C)|FD-Score:6.62|P-value:1.75E-11||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:FRA1(YLL029W)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:FRA2(YGL220W)|FD-Score:4.66|P-value:1.54E-6||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:HAP4(YKL109W)|FD-Score:-3.93|P-value:4.33E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HFA1(YMR207C)|FD-Score:-4.35|P-value:6.75E-6||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HOF1(YMR032W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:IFM1(YOL023W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Mitochondrial translation initiation factor 2 Gene:INP2(YMR163C)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene Gene:IRC5(YFR038W)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:KKQ8(YKL168C)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:KNH1(YDL049C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:LIA1(YJR070C)|FD-Score:5.15|P-value:1.28E-7||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MAC1(YMR021C)|FD-Score:4.8|P-value:8.06E-7||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MBB1(YJL199C_d)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Gene:MDH1(YKL085W)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:MDM35(YKL053C-A)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MDY2(YOL111C)|FD-Score:3.87|P-value:5.38E-5||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MIG3(YER028C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MLP2(YIL149C)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:NBA1(YOL070C)|FD-Score:5.21|P-value:9.55E-8||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:NRT1(YOR071C)|FD-Score:-4.51|P-value:3.20E-6||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:OPI1(YHL020C)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:PEX22(YAL055W)|FD-Score:-3.49|P-value:2.38E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PHO85(YPL031C)|FD-Score:3.84|P-value:6.15E-5||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PMS1(YNL082W)|FD-Score:-3.75|P-value:8.71E-5||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PRS2(YER099C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:-3.85|P-value:5.91E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RFX1(YLR176C)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RGM1(YMR182C)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y-prime telomeric elements and subtelomeric COS genes; RGM1 has a paralog, USV1, that arose from the whole genome duplication Gene:RIM21(YNL294C)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPL27A(YHR010W)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPS21A(YKR057W)|FD-Score:5.81|P-value:3.17E-9||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication Gene:RTT107(YHR154W)|FD-Score:5.79|P-value:3.56E-9||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SAM37(YMR060C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SNF2(YOR290C)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:STE20(YHL007C)|FD-Score:-4.43|P-value:4.61E-6||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:SUR4(YLR372W)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWD3(YBR175W)|FD-Score:14.7|P-value:6.19E-49||SGD DESC:Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 Gene:SYH1(YPL105C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:-3.93|P-value:4.33E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:URM1(YIL008W)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:UTP30(YKR060W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VAC14(YLR386W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VHS2(YIL135C)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p Gene:VID30(YGL227W)|FD-Score:3.78|P-value:7.96E-5||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:VPS72(YDR485C)|FD-Score:5.67|P-value:7.01E-9||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YCR025C(YCR025C_d)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YCR085W(YCR085W_d)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR090C(YCR090C_p)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YGL007C-A(YGL007C-A_p)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGL015C(YGL015C_p)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YIR035C(YIR035C_p)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YKL091C(YKL091C)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YML012C-A(YML012C-A_d)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR316C-B(YMR316C-B_d)|FD-Score:3.91|P-value:4.70E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR052C(YOR052C)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOR263C(YOR263C_d)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W Gene:YPR014C(YPR014C_d)|FD-Score:5.45|P-value:2.46E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR109W(YPR109W_p)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:ZRT2(YLR130C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor Gene:ACE2(YLR131C)|FD-Score:5.98|P-value:1.13E-9||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADY2(YCR010C)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:ANS1(YHR126C_p)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:APS1(YLR170C)|FD-Score:-5.13|P-value:1.43E-7||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ATG22(YCL038C)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:BFR1(YOR198C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BRE5(YNR051C)|FD-Score:5.28|P-value:6.64E-8||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CDC26(YFR036W)|FD-Score:10.1|P-value:3.07E-24||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CIT1(YNR001C)|FD-Score:4.08|P-value:2.30E-5||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:DUG3(YNL191W)|FD-Score:-4.21|P-value:1.30E-5||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EMP70(YLR083C)|FD-Score:-5.57|P-value:1.29E-8||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:ENV7(YPL236C)|FD-Score:-4.12|P-value:1.91E-5||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:EOS1(YNL080C)|FD-Score:4.31|P-value:8.25E-6||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:ERV41(YML067C)|FD-Score:6.62|P-value:1.75E-11||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:FRA1(YLL029W)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:FRA2(YGL220W)|FD-Score:4.66|P-value:1.54E-6||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:HAP4(YKL109W)|FD-Score:-3.93|P-value:4.33E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HFA1(YMR207C)|FD-Score:-4.35|P-value:6.75E-6||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HOF1(YMR032W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:IFM1(YOL023W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Mitochondrial translation initiation factor 2 Gene:INP2(YMR163C)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene Gene:IRC5(YFR038W)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:KKQ8(YKL168C)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:KNH1(YDL049C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:LIA1(YJR070C)|FD-Score:5.15|P-value:1.28E-7||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MAC1(YMR021C)|FD-Score:4.8|P-value:8.06E-7||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MBB1(YJL199C_d)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Gene:MDH1(YKL085W)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:MDM35(YKL053C-A)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MDY2(YOL111C)|FD-Score:3.87|P-value:5.38E-5||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MIG3(YER028C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MLP2(YIL149C)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:NBA1(YOL070C)|FD-Score:5.21|P-value:9.55E-8||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:NRT1(YOR071C)|FD-Score:-4.51|P-value:3.20E-6||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:OPI1(YHL020C)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:PEX22(YAL055W)|FD-Score:-3.49|P-value:2.38E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PHO85(YPL031C)|FD-Score:3.84|P-value:6.15E-5||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PMS1(YNL082W)|FD-Score:-3.75|P-value:8.71E-5||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PRS2(YER099C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:-3.85|P-value:5.91E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RFX1(YLR176C)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RGM1(YMR182C)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y-prime telomeric elements and subtelomeric COS genes; RGM1 has a paralog, USV1, that arose from the whole genome duplication Gene:RIM21(YNL294C)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPL27A(YHR010W)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPS21A(YKR057W)|FD-Score:5.81|P-value:3.17E-9||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication Gene:RTT107(YHR154W)|FD-Score:5.79|P-value:3.56E-9||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SAM37(YMR060C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SNF2(YOR290C)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:STE20(YHL007C)|FD-Score:-4.43|P-value:4.61E-6||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:SUR4(YLR372W)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWD3(YBR175W)|FD-Score:14.7|P-value:6.19E-49||SGD DESC:Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 Gene:SYH1(YPL105C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:-3.93|P-value:4.33E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:URM1(YIL008W)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:UTP30(YKR060W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VAC14(YLR386W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VHS2(YIL135C)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p Gene:VID30(YGL227W)|FD-Score:3.78|P-value:7.96E-5||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:VPS72(YDR485C)|FD-Score:5.67|P-value:7.01E-9||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YCR025C(YCR025C_d)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YCR085W(YCR085W_d)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR090C(YCR090C_p)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YGL007C-A(YGL007C-A_p)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGL015C(YGL015C_p)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YIR035C(YIR035C_p)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YKL091C(YKL091C)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YML012C-A(YML012C-A_d)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR316C-B(YMR316C-B_d)|FD-Score:3.91|P-value:4.70E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR052C(YOR052C)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOR263C(YOR263C_d)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W Gene:YPR014C(YPR014C_d)|FD-Score:5.45|P-value:2.46E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR109W(YPR109W_p)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:ZRT2(YLR130C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W6.786.12E-121.30LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YHR072W5.472.21E-80.32ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YHR170W5.151.28E-70.25NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YHR023W4.904.73E-70.18MYO1Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively
YBR136W4.721.18E-60.02MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YOR074C4.701.31E-60.20CDC21Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S
YGL055W4.503.39E-60.39OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YDR280W4.111.94E-50.12RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YJL111W3.993.25E-50.27CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YOR046C3.729.93E-50.20DBP5Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p
YIL150C3.522.18E-40.02MCM10Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
YGL247W3.502.33E-40.10BRR6Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
YJL014W3.393.44E-40.03CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YNL113W3.363.87E-40.11RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YJR093C3.255.69E-40.17FIP1Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR175W14.706.19E-49SWD3Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5
YFR036W10.103.07E-24CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YML067C6.621.75E-11ERV41Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein
YLR131C5.981.13E-9ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YKR057W5.813.17E-9RPS21AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication
YHR154W5.793.56E-9RTT107Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress
YDR485C5.677.01E-9VPS72Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
YPR014C_d5.452.46E-8YPR014C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene
YNR051C5.286.64E-8BRE5Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A
YOL070C5.219.55E-8NBA1Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate
YJR070C5.151.28E-7LIA1Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress
YMR021C4.808.06E-7MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YGL220W4.661.54E-6FRA2Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel
YCR025C_d4.582.36E-6YCR025C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene
YMR182C4.415.11E-6RGM1Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y-prime telomeric elements and subtelomeric COS genes; RGM1 has a paralog, USV1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1755
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1232.79E-21SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.072289260S ribosome export
0.1141.59E-18SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.081632760S ribosome export
0.1071.88E-16SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.07560S ribosome export
0.1031.98E-15SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.084337360S ribosome export
0.1025.19E-15SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.065789560S ribosome export
0.1016.51E-15SGTC_5794491-1009 110.0 μMChemDiv (Drug-like library)12710410.10810860S ribosome export
0.0953.33E-13SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.063291160S ribosome export
0.0943.85E-13SGTC_29839022671 13.0 μMChembridge (Drug-like library)64051610.13186860S ribosome export
0.0937.53E-13SGTC_8610443-0273 110.0 μMChemDiv (Drug-like library)61491690.0379747
0.0904.94E-12SGTC_1603453-0671 289.7 μMChemDiv (Drug-like library)53222680.0612245
0.0862.91E-11SGTC_32769139060 49.5 μMChembridge (Drug-like library)48706190.160S ribosome export
0.0862.99E-11SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.065217460S ribosome export
0.0841.15E-10SGTC_403arphamenine a 50.0 μMMiscellaneous54871580.057471360S ribosome export
0.0841.27E-10SGTC_1971st069429 63.5 μMTimTec (Natural product derivative library)12465220.11111160S ribosome export
0.0831.57E-10SGTC_9721319-0219 195.0 μMChemDiv (Drug-like library)239037020.0808081

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1819st05344858.6 μM0.5964912857616TimTec (Natural product derivative library)300.378743.44715amide catabolism
SGTC_1641st00929947.1 μM0.4032264046054TimTec (Natural product derivative library)290.771243.46814mitochondrial processes
SGTC_1769st04440664.4 μM0.369231670903TimTec (Natural product derivative library)310.3487433.5415
SGTC_2862902763471.43 μM0.2837845012562Chembridge (Drug-like library)324.416764.45213ERG2
SGTC_23809035439200 μM0.2807026462575Chembridge (Fragment library)201.693142.33922endomembrane recycling
SGTC_3273913785949.47 μM0.2461542377234Chembridge (Drug-like library)266.360764.53803
SGTC_2936903823515.59 μM0.2432434749415Chembridge (Drug-like library)330.404723.48406
SGTC_412gemfibrozil100 μM0.2173913463Miscellaneous250.333384.17213DNA intercalators
SGTC_22157188065200 μM0.216667940217Chembridge (Fragment library)178.618282.74702
SGTC_14133910-053534.4 μM0.21252876951ChemDiv (Drug-like library)358.861825.21313