st041558

ethyl 2-[[2-[4-(2-benzamido-3-methoxy-3-oxopropyl)phenoxy]acetyl]amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1756
Screen concentration 35.5 μM
Source TimTec (Natural product derivative library)
PubChem CID 3955792
SMILES CCOC(=O)C1=C(SC2=C1CCCC2)NC(=O)COC3=CC=C(C=C3)CC(C(=O)OC)NC(=O)C4=CC=CC=C4
Standardized SMILES CCOC(=O)c1c2CCCCc2sc1NC(=O)COc3ccc(CC(NC(=O)c4ccccc4)C(=O)OC)cc3
Molecular weight 564.6493
ALogP 4.78
H-bond donor count 2
H-bond acceptor count 8
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.7
% growth inhibition (Hom. pool) 2.17


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3955792
Download HIP data (tab-delimited text)  (excel)
Gene:ADE13(YLR359W)|FD-Score:-3.91|P-value:4.55E-5|Clearance:0||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:BET5(YML077W)|FD-Score:3.88|P-value:5.19E-5|Clearance:0.41||SGD DESC:Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi Gene:CCT7(YJL111W)|FD-Score:-6.36|P-value:1.01E-10|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:ERB1(YMR049C)|FD-Score:3.22|P-value:6.41E-4|Clearance:0.41||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:ERG12(YMR208W)|FD-Score:4.03|P-value:2.84E-5|Clearance:0.41||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GRS1(YBR121C)|FD-Score:4.13|P-value:1.85E-5|Clearance:0.41||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:IDI1(YPL117C)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.41||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:NIC96(YFR002W)|FD-Score:3.19|P-value:7.14E-4|Clearance:0.41||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:POP3(YNL282W)|FD-Score:-3.26|P-value:5.56E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC61(YLR378C)|FD-Score:-3.17|P-value:7.72E-4|Clearance:0||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:SEC9(YGR009C)|FD-Score:3.31|P-value:4.62E-4|Clearance:0.41||SGD DESC:t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog Gene:SPC97(YHR172W)|FD-Score:3.29|P-value:4.94E-4|Clearance:0.41||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SPP381(YBR152W)|FD-Score:-3.2|P-value:6.98E-4|Clearance:0||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:TAF7(YMR227C)|FD-Score:-3.4|P-value:3.37E-4|Clearance:0||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:UBC9(YDL064W)|FD-Score:4.23|P-value:1.15E-5|Clearance:0.41||SGD DESC:SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) Gene:UFE1(YOR075W)|FD-Score:4.22|P-value:1.20E-5|Clearance:0.41||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:ADE13(YLR359W)|FD-Score:-3.91|P-value:4.55E-5|Clearance:0||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:BET5(YML077W)|FD-Score:3.88|P-value:5.19E-5|Clearance:0.41||SGD DESC:Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi Gene:CCT7(YJL111W)|FD-Score:-6.36|P-value:1.01E-10|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:ERB1(YMR049C)|FD-Score:3.22|P-value:6.41E-4|Clearance:0.41||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:ERG12(YMR208W)|FD-Score:4.03|P-value:2.84E-5|Clearance:0.41||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GRS1(YBR121C)|FD-Score:4.13|P-value:1.85E-5|Clearance:0.41||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:IDI1(YPL117C)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.41||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:NIC96(YFR002W)|FD-Score:3.19|P-value:7.14E-4|Clearance:0.41||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:POP3(YNL282W)|FD-Score:-3.26|P-value:5.56E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC61(YLR378C)|FD-Score:-3.17|P-value:7.72E-4|Clearance:0||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:SEC9(YGR009C)|FD-Score:3.31|P-value:4.62E-4|Clearance:0.41||SGD DESC:t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog Gene:SPC97(YHR172W)|FD-Score:3.29|P-value:4.94E-4|Clearance:0.41||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SPP381(YBR152W)|FD-Score:-3.2|P-value:6.98E-4|Clearance:0||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:TAF7(YMR227C)|FD-Score:-3.4|P-value:3.37E-4|Clearance:0||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:UBC9(YDL064W)|FD-Score:4.23|P-value:1.15E-5|Clearance:0.41||SGD DESC:SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) Gene:UFE1(YOR075W)|FD-Score:4.22|P-value:1.20E-5|Clearance:0.41||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3955792
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADY3(YDL239C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Protein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication Gene:ALB1(YJL122W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:ARI1(YGL157W)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:ATX1(YNL259C)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:BOP3(YNL042W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury Gene:BUD2(YKL092C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint Gene:CUP9(YPL177C)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:ECM19(YLR390W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FET5(YFL041W)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport Gene:GMC1(YDR506C)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:GRH1(YDR517W)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress Gene:HBT1(YDL223C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HSM3(YBR272C)|FD-Score:5.94|P-value:1.44E-9||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:HSP12(YFL014W)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension Gene:IKS1(YJL057C)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p Gene:IRC13(YOR235W_d)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:ITR1(YDR497C)|FD-Score:5.24|P-value:7.83E-8||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:KNH1(YDL049C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:MET16(YPR167C)|FD-Score:3.83|P-value:6.28E-5||SGD DESC:3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism Gene:NUR1(YDL089W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:PAU24(YBR301W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PEX18(YHR160C)|FD-Score:-3.53|P-value:2.07E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PKP2(YGL059W)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:PMA2(YPL036W)|FD-Score:4.57|P-value:2.48E-6||SGD DESC:Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential Gene:SER3(YER081W)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SPE1(YKL184W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Ornithine decarboxylase, catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation Gene:SRO77(YBL106C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SSA3(YBL075C)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:TAE1(YBR261C)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm Gene:TSR2(YLR435W)|FD-Score:-4.57|P-value:2.43E-6||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:UFD4(YKL010C)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:UTP30(YKR060W)|FD-Score:-4.71|P-value:1.22E-6||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VPS9(YML097C)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YGL149W(YGL149W_d)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YKL202W(YKL202W_d)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR269C(YLR269C_d)|FD-Score:4.47|P-value:3.86E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR349W(YLR349W_d)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C Gene:YOL131W(YOL131W_p)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Putative protein of unknown function Gene:ACB1(YGR037C)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADY3(YDL239C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Protein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication Gene:ALB1(YJL122W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:ARI1(YGL157W)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:ATX1(YNL259C)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:BOP3(YNL042W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury Gene:BUD2(YKL092C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint Gene:CUP9(YPL177C)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:ECM19(YLR390W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FET5(YFL041W)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport Gene:GMC1(YDR506C)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:GRH1(YDR517W)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress Gene:HBT1(YDL223C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HSM3(YBR272C)|FD-Score:5.94|P-value:1.44E-9||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:HSP12(YFL014W)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension Gene:IKS1(YJL057C)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p Gene:IRC13(YOR235W_d)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:ITR1(YDR497C)|FD-Score:5.24|P-value:7.83E-8||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:KNH1(YDL049C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:MET16(YPR167C)|FD-Score:3.83|P-value:6.28E-5||SGD DESC:3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism Gene:NUR1(YDL089W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:PAU24(YBR301W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PEX18(YHR160C)|FD-Score:-3.53|P-value:2.07E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PKP2(YGL059W)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:PMA2(YPL036W)|FD-Score:4.57|P-value:2.48E-6||SGD DESC:Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential Gene:SER3(YER081W)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SPE1(YKL184W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Ornithine decarboxylase, catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation Gene:SRO77(YBL106C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SSA3(YBL075C)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:TAE1(YBR261C)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm Gene:TSR2(YLR435W)|FD-Score:-4.57|P-value:2.43E-6||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:UFD4(YKL010C)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:UTP30(YKR060W)|FD-Score:-4.71|P-value:1.22E-6||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VPS9(YML097C)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YGL149W(YGL149W_d)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YKL202W(YKL202W_d)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR269C(YLR269C_d)|FD-Score:4.47|P-value:3.86E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR349W(YLR349W_d)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C Gene:YOL131W(YOL131W_p)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL064W4.231.15E-50.41UBC9SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC)
YOR075W4.221.20E-50.41UFE1t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER
YBR121C4.131.85E-50.41GRS1Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication
YMR208W4.032.84E-50.41ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YML077W3.885.19E-50.41BET5Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi
YPL117C3.532.08E-40.41IDI1Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability
YGR009C3.314.62E-40.41SEC9t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog
YHR172W3.294.94E-40.41SPC97Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YMR049C3.226.41E-40.41ERB1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1
YFR002W3.197.14E-40.41NIC96Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
YNL151C2.780.002710.04RPC31RNA polymerase III subunit C31
YNL110C2.740.003070.06NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YLR132C2.680.003650.01USB1Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants
YGL068W2.670.003760.10MNP1Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth
YDL098C2.580.004970.03SNU23Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR272C5.941.44E-9HSM3Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation
YDR497C5.247.83E-8ITR1Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress
YBL075C4.582.33E-6SSA3ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication
YGL149W_d4.572.43E-6YGL149W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C.
YPL036W4.572.48E-6PMA2Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential
YLR269C_d4.473.86E-6YLR269C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPL177C4.201.33E-5CUP9Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YFL014W4.191.37E-5HSP12Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
YBL106C4.121.89E-5SRO77Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication
YDL049C4.072.34E-5KNH1Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
YBR261C4.062.47E-5TAE1AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm
YGR037C4.062.48E-5ACB1Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
YOL131W_p3.944.14E-5YOL131W_pPutative protein of unknown function
YPR167C3.836.28E-5MET163'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism
YDL239C3.691.13E-4ADY3Protein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1756
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1453.29E-29SGTC_1753st043925 64.0 μMTimTec (Natural product derivative library)6701810.132653
0.0613.26E-6SGTC_29177979103 53.8 μMChembridge (Drug-like library)29805650.150538
0.0587.22E-6SGTC_31569099931 49.5 μMChembridge (Drug-like library)181110530.147368
0.0588.96E-6SGTC_30409091894 49.5 μMChembridge (Drug-like library)252369660.168421DNA intercalators
0.0571.30E-5SGTC_3700180-0345 38.7 μMChemDiv (Drug-like library)39895270.0879121DNA intercalators
0.0543.33E-5SGTC_30169080866 49.5 μMChembridge (Drug-like library)170662990.15534
0.0518.71E-5SGTC_512prima-1 270.0 μMICCB bioactive library3229680.0454545DNA intercalators
0.0491.63E-4SGTC_14063909-7906 11.9 μMChemDiv (Drug-like library)X14060.1010160S ribosome export
0.0482.27E-4SGTC_774k060-0086 136.0 μMChemDiv (Drug-like library)59112350.07
0.0472.67E-4SGTC_14053909-7894 12.0 μMChemDiv (Drug-like library)71040600.10309360S ribosome export
0.0472.68E-4SGTC_24325630864 12.2 μMMiscellaneous57149700.0824742
0.0472.98E-4SGTC_2803448-3607 3.0 μMChemDiv (Drug-like library)6261790.088235360S ribosome export
0.0473.01E-4SGTC_1880MG-132 525.0 μMMiscellaneous4623820.193548
0.0473.02E-4SGTC_2416tofa 880.0 nMMiscellaneous1151750.122222sphingolipid biosynthesis & PDR1
0.0464.10E-4SGTC_4711-acyl-paf 4.0 μMICCB bioactive library167593670.0849057DNA intercalators

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1704st02880570.8 μM0.4459462876101TimTec (Natural product derivative library)282.358622.5724
SGTC_2937904475311.33 μM0.43756465530Chembridge (Drug-like library)393.543443.0921660S ribosome export
SGTC_10134112-3890241 μM0.385542705171ChemDiv (Drug-like library)331.386183.39725TSC3-RPN4
SGTC_309k408-00511.48 μM0.3655913640463ChemDiv (Drug-like library)505.650043.32218
SGTC_8034488-1585147 μM0.349398747204ChemDiv (Drug-like library)315.386783.924
SGTC_9751335-004628.5 μM0.34523816486740ChemDiv (Drug-like library)374.250142.67915RPP1 & pyrimidine depletion
SGTC_2833900131545.46 μM0.3372092989378Chembridge (Drug-like library)358.411522.55425amide catabolism
SGTC_1694st02401144.9 μM0.329672787533TimTec (Natural product derivative library)445.306444.01124ERG2
SGTC_9133487-1245167 μM0.3214294016153ChemDiv (Drug-like library)349.831844.53424
SGTC_3216912971649.47 μM0.3214298056934Chembridge (Drug-like library)310.368721.40815RPP1 & pyrimidine depletion