st042942

methyl 2-[(3,4-dichlorobenzoyl)amino]acetate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1758
Screen concentration 75.2 μM
Source TimTec (Natural product derivative library)
PubChem CID 669317
SMILES COC(=O)CNC(=O)C1=CC(=C(C=C1)Cl)Cl
Standardized SMILES COC(=O)CNC(=O)c1ccc(Cl)c(Cl)c1
Molecular weight 262.0894
ALogP 2.07
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.16
% growth inhibition (Hom. pool) 8.55


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 669317
Download HIP data (tab-delimited text)  (excel)
Gene:ADE13(YLR359W)|FD-Score:3.4|P-value:3.35E-4|Clearance:0.07||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:ERB1(YMR049C)|FD-Score:-3.23|P-value:6.22E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:ERG1(YGR175C)|FD-Score:3.34|P-value:4.24E-4|Clearance:0.15||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:ERG12(YMR208W)|FD-Score:3.09|P-value:9.99E-4|Clearance:0.11||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG9(YHR190W)|FD-Score:4.47|P-value:4.00E-6|Clearance:0.35||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:FAP7(YDL166C)|FD-Score:-4.47|P-value:3.95E-6|Clearance:0||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:ILS1(YBL076C)|FD-Score:3.75|P-value:8.90E-5|Clearance:0.16||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:NOP1(YDL014W)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NSP1(YJL041W)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:RPO26(YPR187W)|FD-Score:3.19|P-value:7.15E-4|Clearance:0.1||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:SEC27(YGL137W)|FD-Score:3.81|P-value:7.04E-5|Clearance:0.06||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SPT6(YGR116W)|FD-Score:4.12|P-value:1.91E-5|Clearance:0.31||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:YPR136C(YPR136C_d)|FD-Score:3.58|P-value:1.69E-4|Clearance:0.18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:ADE13(YLR359W)|FD-Score:3.4|P-value:3.35E-4|Clearance:0.07||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:ERB1(YMR049C)|FD-Score:-3.23|P-value:6.22E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:ERG1(YGR175C)|FD-Score:3.34|P-value:4.24E-4|Clearance:0.15||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:ERG12(YMR208W)|FD-Score:3.09|P-value:9.99E-4|Clearance:0.11||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG9(YHR190W)|FD-Score:4.47|P-value:4.00E-6|Clearance:0.35||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:FAP7(YDL166C)|FD-Score:-4.47|P-value:3.95E-6|Clearance:0||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:ILS1(YBL076C)|FD-Score:3.75|P-value:8.90E-5|Clearance:0.16||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:NOP1(YDL014W)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NSP1(YJL041W)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:RPO26(YPR187W)|FD-Score:3.19|P-value:7.15E-4|Clearance:0.1||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:SEC27(YGL137W)|FD-Score:3.81|P-value:7.04E-5|Clearance:0.06||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SPT6(YGR116W)|FD-Score:4.12|P-value:1.91E-5|Clearance:0.31||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:YPR136C(YPR136C_d)|FD-Score:3.58|P-value:1.69E-4|Clearance:0.18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 669317
Download HOP data (tab-delimited text)  (excel)
Gene:AEP2(YMR282C)|FD-Score:6.88|P-value:3.03E-12||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:ARN2(YHL047C)|FD-Score:-3.12|P-value:9.12E-4||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:ATP12(YJL180C)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:CBP4(YGR174C)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:CCM1(YGR150C)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CMC4(YMR194C-B)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:CMR3(YPR013C_p)|FD-Score:-4.84|P-value:6.44E-7||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:DBF2(YGR092W)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DJP1(YIR004W)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:DLD2(YDL178W)|FD-Score:-3.82|P-value:6.73E-5||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:DUG3(YNL191W)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ELP2(YGR200C)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:GEP5(YLR091W)|FD-Score:4.27|P-value:9.97E-6||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GIC1(YHR061C)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress Gene:HDA3(YPR179C)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p Gene:HLJ1(YMR161W)|FD-Score:-3.21|P-value:6.64E-4||SGD DESC:Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ Gene:HOS3(YPL116W)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats Gene:HSM3(YBR272C)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:IMA5(YJL216C)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose Gene:IMO32(YGR031W)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:IRC23(YOR044W)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:IRC2(YDR112W_d)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:MED1(YPR070W)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MMS2(YGL087C)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MPP6(YNR024W)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRPS16(YPL013C)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSI1(YBR195C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:MSS18(YPR134W)|FD-Score:-3.09|P-value:9.86E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:MTG1(YMR097C)|FD-Score:4.39|P-value:5.57E-6||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NRG1(YDR043C)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; NRG1 has a paralog, NRG2, that arose from the whole genome duplication Gene:OCT1(YKL134C)|FD-Score:5.62|P-value:9.81E-9||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:PDE2(YOR360C)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon Gene:PET111(YMR257C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PEX13(YLR191W)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PHD1(YKL043W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication Gene:POP2(YNR052C)|FD-Score:-3.1|P-value:9.59E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PPS1(YBR276C)|FD-Score:5.09|P-value:1.76E-7||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:REV7(YIL139C)|FD-Score:3.97|P-value:3.61E-5||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RIC1(YLR039C)|FD-Score:-5.89|P-value:1.95E-9||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL19B(YBL027W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RSM24(YDR175C)|FD-Score:4.89|P-value:5.11E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SAC6(YDR129C)|FD-Score:-4.08|P-value:2.24E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEC66(YBR171W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIN4(YNL236W)|FD-Score:-3.74|P-value:9.22E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SIT4(YDL047W)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SKT5(YBL061C)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SLA1(YBL007C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLK19(YOR195W)|FD-Score:5.29|P-value:6.17E-8||SGD DESC:Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate Gene:SPS19(YNL202W)|FD-Score:-3.82|P-value:6.71E-5||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:TAE2(YPL009C)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TCB2(YNL087W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TRM44(YPL030W)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:VHS2(YIL135C)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p Gene:VPS69(YPR087W_d)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YBR016W(YBR016W)|FD-Score:5.36|P-value:4.12E-8||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YDR220C(YDR220C_d)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YER066C-A(YER066C-A_d)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W Gene:YGL006W-A(YGL006W-A_p)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YGL081W(YGL081W_p)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis Gene:YGR126W(YGR126W_p)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YHR131C(YHR131C_p)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study Gene:YIL025C(YIL025C_d)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR031W(YLR031W_p)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Putative protein of unknown function Gene:YLR460C(YLR460C_p)|FD-Score:-3.13|P-value:8.61E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YML047W-A(YML047W-A_d)|FD-Score:5.25|P-value:7.76E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR018W(YMR018W_p)|FD-Score:5.43|P-value:2.89E-8||SGD DESC:Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene Gene:YMR178W(YMR178W_p)|FD-Score:5.81|P-value:3.17E-9||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; YMR178W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR209C(YMR209C_p)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YNL190W(YNL190W_p)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YPR117W(YPR117W_p)|FD-Score:3.79|P-value:7.68E-5||SGD DESC:Putative protein of unknown function Gene:AEP2(YMR282C)|FD-Score:6.88|P-value:3.03E-12||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:ARN2(YHL047C)|FD-Score:-3.12|P-value:9.12E-4||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:ATP12(YJL180C)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:CBP4(YGR174C)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:CCM1(YGR150C)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CMC4(YMR194C-B)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:CMR3(YPR013C_p)|FD-Score:-4.84|P-value:6.44E-7||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:DBF2(YGR092W)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DJP1(YIR004W)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:DLD2(YDL178W)|FD-Score:-3.82|P-value:6.73E-5||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:DUG3(YNL191W)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ELP2(YGR200C)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:GEP5(YLR091W)|FD-Score:4.27|P-value:9.97E-6||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GIC1(YHR061C)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress Gene:HDA3(YPR179C)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p Gene:HLJ1(YMR161W)|FD-Score:-3.21|P-value:6.64E-4||SGD DESC:Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ Gene:HOS3(YPL116W)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats Gene:HSM3(YBR272C)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:IMA5(YJL216C)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose Gene:IMO32(YGR031W)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:IRC23(YOR044W)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:IRC2(YDR112W_d)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:MED1(YPR070W)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MMS2(YGL087C)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MPP6(YNR024W)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRPS16(YPL013C)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSI1(YBR195C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:MSS18(YPR134W)|FD-Score:-3.09|P-value:9.86E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:MTG1(YMR097C)|FD-Score:4.39|P-value:5.57E-6||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NRG1(YDR043C)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; NRG1 has a paralog, NRG2, that arose from the whole genome duplication Gene:OCT1(YKL134C)|FD-Score:5.62|P-value:9.81E-9||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:PDE2(YOR360C)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon Gene:PET111(YMR257C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PEX13(YLR191W)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PHD1(YKL043W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication Gene:POP2(YNR052C)|FD-Score:-3.1|P-value:9.59E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PPS1(YBR276C)|FD-Score:5.09|P-value:1.76E-7||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:REV7(YIL139C)|FD-Score:3.97|P-value:3.61E-5||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RIC1(YLR039C)|FD-Score:-5.89|P-value:1.95E-9||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL19B(YBL027W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RSM24(YDR175C)|FD-Score:4.89|P-value:5.11E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SAC6(YDR129C)|FD-Score:-4.08|P-value:2.24E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEC66(YBR171W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIN4(YNL236W)|FD-Score:-3.74|P-value:9.22E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SIT4(YDL047W)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SKT5(YBL061C)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SLA1(YBL007C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLK19(YOR195W)|FD-Score:5.29|P-value:6.17E-8||SGD DESC:Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate Gene:SPS19(YNL202W)|FD-Score:-3.82|P-value:6.71E-5||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:TAE2(YPL009C)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TCB2(YNL087W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TRM44(YPL030W)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:VHS2(YIL135C)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p Gene:VPS69(YPR087W_d)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YBR016W(YBR016W)|FD-Score:5.36|P-value:4.12E-8||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YDR220C(YDR220C_d)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YER066C-A(YER066C-A_d)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W Gene:YGL006W-A(YGL006W-A_p)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YGL081W(YGL081W_p)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis Gene:YGR126W(YGR126W_p)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YHR131C(YHR131C_p)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study Gene:YIL025C(YIL025C_d)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR031W(YLR031W_p)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Putative protein of unknown function Gene:YLR460C(YLR460C_p)|FD-Score:-3.13|P-value:8.61E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YML047W-A(YML047W-A_d)|FD-Score:5.25|P-value:7.76E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR018W(YMR018W_p)|FD-Score:5.43|P-value:2.89E-8||SGD DESC:Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene Gene:YMR178W(YMR178W_p)|FD-Score:5.81|P-value:3.17E-9||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; YMR178W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR209C(YMR209C_p)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YNL190W(YNL190W_p)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YPR117W(YPR117W_p)|FD-Score:3.79|P-value:7.68E-5||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR190W4.474.00E-60.35ERG9Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
YGR116W4.121.91E-50.31SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YGL137W3.817.04E-50.06SEC27Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
YBL076C3.758.90E-50.16ILS1Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A
YPR136C_d3.581.69E-40.18YPR136C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
YLR359W3.403.35E-40.07ADE13Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
YGR175C3.344.24E-40.15ERG1Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
YPR187W3.197.15E-40.10RPO26RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
YMR208W3.099.99E-40.11ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YLR066W2.980.001450.00SPC3Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23
YDL193W2.980.001460.02NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YMR235C2.960.001550.00RNA1GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport
YPR186C2.950.001570.01PZF1Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
YML077W2.940.001620.07BET5Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi
YPL218W2.870.002050.03SAR1GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR282C6.883.03E-12AEP2Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader
YMR178W_p5.813.17E-9YMR178W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; YMR178W is not an essential gene; protein abundance increases in response to DNA replication stress
YKL134C5.629.81E-9OCT1Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis
YMR018W_p5.432.89E-8YMR018W_pPutative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene
YBR016W5.364.12E-8YBR016WTail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication
YOR195W5.296.17E-8SLK19Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate
YML047W-A_d5.257.76E-8YML047W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBR276C5.091.76E-7PPS1Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle
YDR175C4.895.11E-7RSM24Mitochondrial ribosomal protein of the small subunit
YNR024W4.542.85E-6MPP6Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes
YMR194C-B4.503.33E-6CMC4Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs
YMR097C4.395.57E-6MTG1Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals
YLR091W4.279.97E-6GEP5Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine
YIL025C_d4.131.83E-5YIL025C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPL030W4.121.90E-5TRM44tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene

GO enrichment analysis for SGTC_1758
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0815.82E-10SGTC_1731273-0061 2.7 μMChemDiv (Drug-like library)28474140.0819672mitochondrial stress
0.0757.72E-9SGTC_13712421-0008 32.7 μMChemDiv (Drug-like library)28275520.0757576mitochondrial processes
0.0664.34E-7SGTC_3101273-0058 1.1 μMChemDiv (Drug-like library)27342320.0555556mitochondrial stress
0.0656.21E-7SGTC_9953931-2021 34.6 μMChemDiv (Drug-like library)7301430.114286RPP1 & pyrimidine depletion
0.0648.26E-7SGTC_2361509-0011 5.3 μMChemDiv (Drug-like library)31072800.0819672
0.0595.99E-6SGTC_8324083-0019 164.0 μMChemDiv (Drug-like library)19907580.115385translation
0.0571.14E-5SGTC_1856st057054 55.7 μMTimTec (Natural product derivative library)12660180.161765
0.0561.66E-5SGTC_1620st003704 16.5 μMTimTec (Natural product derivative library)28344140.125
0.0561.70E-5SGTC_275trichlorophene 5.7 μMMiscellaneous626160.142857mitochondrial stress
0.0552.71E-5SGTC_1010490-5701 117.5 μMChemDiv (Drug-like library)46616690.181818
0.0534.45E-5SGTC_9083448-7491 47.7 μMChemDiv (Drug-like library)12030310.0877193mitochondrial stress
0.0534.64E-5SGTC_100279-0212 42.6 μMChemDiv (Drug-like library)398510.14TRP & mitochondrial translation
0.0518.43E-5SGTC_6010986-0029 62.9 μMChemDiv (Drug-like library)247479810.152542calcium & mitochondrial duress
0.0518.62E-5SGTC_630099-0242 26.7 μMChemDiv (Drug-like library)38985020.0740741mitochondrial stress
0.0501.09E-4SGTC_21836420625 130.5 μMChembridge (Fragment library)28976190.126984mitochondrial processes

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_21535569715200 μM0.5116282054701Chembridge (Fragment library)281.13732.79912iron homeostasis
SGTC_2929901334824.6 μM0.5111112995952Chembridge (Drug-like library)310.175223.99912
SGTC_8480368-008985.6 μM0.404255762535ChemDiv (Drug-like library)267.110722.79212iron homeostasis
SGTC_3118912425049.47 μM0.3653851504786Chembridge (Drug-like library)317.16944.12912
SGTC_12890976-007369.1 μM0.3555563391136ChemDiv (Drug-like library)253.29582.7812
SGTC_7211187-1332279 μM0.34782625730ChemDiv (Drug-like library)281.13733.96522cell wall signaling
SGTC_22537938677135.83 μM0.3478262301972Chembridge (Fragment library)229.660181.66913
SGTC_11710368-007721.4 μM0.3469393239779ChemDiv (Drug-like library)268.095483.4403
SGTC_3310913022944.14 μM0.3396237761258Chembridge (Drug-like library)289.756743.8212Golgi
SGTC_2787528454071.43 μM0.333333736958Chembridge (Drug-like library)291.729563.24613