st044296

2-[4-(4-chlorophenyl)-1-oxophthalazin-2-yl]-N-[2-(6,7-dichloro-2-methyl-1H-indol-3-yl)ethyl]acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1761
Screen concentration 37.0 μM
Source TimTec (Natural product derivative library)
PubChem CID 1826700
SMILES CC1=C(C2=C(N1)C(=C(C=C2)Cl)Cl)CCNC(=O)CN3C(=O)C4=CC=CC=C4C(=N3)C5=CC=C(C=C5)Cl
Standardized SMILES Cc1[nH]c2c(Cl)c(Cl)ccc2c1CCNC(=O)CN3N=C(c4ccc(Cl)cc4)c5ccccc5C3=O
Molecular weight 539.8402
ALogP 6.1
H-bond donor count 2
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.52
% growth inhibition (Hom. pool) 2.22


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1826700
Download HIP data (tab-delimited text)  (excel)
Gene:ARC40(YBR234C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.02||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CDC31(YOR257W)|FD-Score:3.27|P-value:5.38E-4|Clearance:0.17||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CDC39(YCR093W)|FD-Score:-3.36|P-value:3.95E-4|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CTR86(YCR054C)|FD-Score:-3.47|P-value:2.56E-4|Clearance:0||SGD DESC:Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress Gene:GCD2(YGR083C)|FD-Score:3.38|P-value:3.66E-4|Clearance:0.11||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:ISD11(YER048W-A)|FD-Score:-6.9|P-value:2.59E-12|Clearance:0||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:LSM2(YBL026W)|FD-Score:4.1|P-value:2.07E-5|Clearance:0.07||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NOP1(YDL014W)|FD-Score:5.04|P-value:2.32E-7|Clearance:0.18||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NOP9(YJL010C)|FD-Score:-3.4|P-value:3.34E-4|Clearance:0||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:PDS5(YMR076C)|FD-Score:3.47|P-value:2.60E-4|Clearance:0.02||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PFY1(YOR122C)|FD-Score:-4.24|P-value:1.09E-5|Clearance:0||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:RPB7(YDR404C)|FD-Score:4.03|P-value:2.78E-5|Clearance:0.36||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPL18A(YOL120C)|FD-Score:6.37|P-value:9.61E-11|Clearance:1.33||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPS20(YHL015W)|FD-Score:3.1|P-value:9.64E-4|Clearance:0.02||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:SPB1(YCL054W)|FD-Score:4.86|P-value:5.95E-7|Clearance:0.76||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:UBA1(YKL210W)|FD-Score:3.45|P-value:2.82E-4|Clearance:0.05||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:YOR146W(YOR146W_d)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.2||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:ARC40(YBR234C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.02||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CDC31(YOR257W)|FD-Score:3.27|P-value:5.38E-4|Clearance:0.17||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CDC39(YCR093W)|FD-Score:-3.36|P-value:3.95E-4|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CTR86(YCR054C)|FD-Score:-3.47|P-value:2.56E-4|Clearance:0||SGD DESC:Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress Gene:GCD2(YGR083C)|FD-Score:3.38|P-value:3.66E-4|Clearance:0.11||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:ISD11(YER048W-A)|FD-Score:-6.9|P-value:2.59E-12|Clearance:0||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:LSM2(YBL026W)|FD-Score:4.1|P-value:2.07E-5|Clearance:0.07||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NOP1(YDL014W)|FD-Score:5.04|P-value:2.32E-7|Clearance:0.18||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NOP9(YJL010C)|FD-Score:-3.4|P-value:3.34E-4|Clearance:0||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:PDS5(YMR076C)|FD-Score:3.47|P-value:2.60E-4|Clearance:0.02||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PFY1(YOR122C)|FD-Score:-4.24|P-value:1.09E-5|Clearance:0||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:RPB7(YDR404C)|FD-Score:4.03|P-value:2.78E-5|Clearance:0.36||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPL18A(YOL120C)|FD-Score:6.37|P-value:9.61E-11|Clearance:1.33||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPS20(YHL015W)|FD-Score:3.1|P-value:9.64E-4|Clearance:0.02||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:SPB1(YCL054W)|FD-Score:4.86|P-value:5.95E-7|Clearance:0.76||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:UBA1(YKL210W)|FD-Score:3.45|P-value:2.82E-4|Clearance:0.05||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:YOR146W(YOR146W_d)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.2||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1826700
Download HOP data (tab-delimited text)  (excel)
Gene:ARN2(YHL047C)|FD-Score:-5.3|P-value:5.68E-8||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:BUD19(YJL188C_d)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:COX16(YJL003W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:CST26(YBR042C)|FD-Score:4|P-value:3.13E-5||SGD DESC:Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication Gene:CTF19(YPL018W)|FD-Score:4|P-value:3.17E-5||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:EAR1(YMR171C)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:EHT1(YBR177C)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:ERG28(YER044C)|FD-Score:-3.15|P-value:8.13E-4||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:FYV4(YHR059W)|FD-Score:7.29|P-value:1.56E-13||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GOS1(YHL031C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GPD2(YOL059W)|FD-Score:4.95|P-value:3.62E-7||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:HNT3(YOR258W)|FD-Score:4.37|P-value:6.23E-6||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:ICE2(YIL090W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IMA1(YGR287C)|FD-Score:4.42|P-value:5.02E-6||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:IRC18(YJL037W)|FD-Score:6.52|P-value:3.44E-11||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:-4.06|P-value:2.47E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IST1(YNL265C)|FD-Score:3.78|P-value:7.98E-5||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:LST4(YKL176C)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LYS5(YGL154C)|FD-Score:-3.18|P-value:7.47E-4||SGD DESC:Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine Gene:MCM16(YPR046W)|FD-Score:4.28|P-value:9.31E-6||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:NDT80(YHR124W)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:NPP1(YCR026C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:NRT1(YOR071C)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:NTA1(YJR062C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation Gene:NUP133(YKR082W)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:NYV1(YLR093C)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane Gene:OCA2(YNL056W)|FD-Score:-4.61|P-value:2.03E-6||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OCT1(YKL134C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:ORM2(YLR350W)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:RAD17(YOR368W)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RAD5(YLR032W)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAV2(YDR202C)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:REI1(YBR267W)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RPL13A(YDL082W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL40B(YKR094C)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RPS27B(YHR021C)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RRP8(YDR083W)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:SHO1(YER118C)|FD-Score:5.62|P-value:9.42E-9||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SHU2(YDR078C)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SPO13(YHR014W)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Meiosis-specific protein, involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II Gene:STE23(YLR389C)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:TED1(YIL039W)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:VPS9(YML097C)|FD-Score:-5.09|P-value:1.78E-7||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YBR113W(YBR113W_d)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YBR141C(YBR141C_p)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YCR007C(YCR007C_p)|FD-Score:-4.78|P-value:8.79E-7||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDL144C(YDL144C_p)|FD-Score:5.24|P-value:8.06E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YDR476C(YDR476C_p)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YFR045W(YFR045W_p)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white Gene:YHR140W(YHR140W_p)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Putative integral membrane protein of unknown function Gene:YIL025C(YIL025C_d)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR022C(YOR022C_p)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR200W(YOR200W_d)|FD-Score:4.9|P-value:4.88E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPR014C(YPR014C_d)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:ARN2(YHL047C)|FD-Score:-5.3|P-value:5.68E-8||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:BUD19(YJL188C_d)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:COX16(YJL003W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:CST26(YBR042C)|FD-Score:4|P-value:3.13E-5||SGD DESC:Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication Gene:CTF19(YPL018W)|FD-Score:4|P-value:3.17E-5||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:EAR1(YMR171C)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:EHT1(YBR177C)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:ERG28(YER044C)|FD-Score:-3.15|P-value:8.13E-4||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:FYV4(YHR059W)|FD-Score:7.29|P-value:1.56E-13||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GOS1(YHL031C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GPD2(YOL059W)|FD-Score:4.95|P-value:3.62E-7||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:HNT3(YOR258W)|FD-Score:4.37|P-value:6.23E-6||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:ICE2(YIL090W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IMA1(YGR287C)|FD-Score:4.42|P-value:5.02E-6||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:IRC18(YJL037W)|FD-Score:6.52|P-value:3.44E-11||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:-4.06|P-value:2.47E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IST1(YNL265C)|FD-Score:3.78|P-value:7.98E-5||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:LST4(YKL176C)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LYS5(YGL154C)|FD-Score:-3.18|P-value:7.47E-4||SGD DESC:Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine Gene:MCM16(YPR046W)|FD-Score:4.28|P-value:9.31E-6||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:NDT80(YHR124W)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:NPP1(YCR026C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:NRT1(YOR071C)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:NTA1(YJR062C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation Gene:NUP133(YKR082W)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:NYV1(YLR093C)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane Gene:OCA2(YNL056W)|FD-Score:-4.61|P-value:2.03E-6||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OCT1(YKL134C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:ORM2(YLR350W)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:RAD17(YOR368W)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RAD5(YLR032W)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAV2(YDR202C)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:REI1(YBR267W)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RPL13A(YDL082W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL40B(YKR094C)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RPS27B(YHR021C)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RRP8(YDR083W)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:SHO1(YER118C)|FD-Score:5.62|P-value:9.42E-9||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SHU2(YDR078C)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SPO13(YHR014W)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Meiosis-specific protein, involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II Gene:STE23(YLR389C)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:TED1(YIL039W)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:VPS9(YML097C)|FD-Score:-5.09|P-value:1.78E-7||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YBR113W(YBR113W_d)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YBR141C(YBR141C_p)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YCR007C(YCR007C_p)|FD-Score:-4.78|P-value:8.79E-7||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDL144C(YDL144C_p)|FD-Score:5.24|P-value:8.06E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YDR476C(YDR476C_p)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YFR045W(YFR045W_p)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white Gene:YHR140W(YHR140W_p)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Putative integral membrane protein of unknown function Gene:YIL025C(YIL025C_d)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR022C(YOR022C_p)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR200W(YOR200W_d)|FD-Score:4.9|P-value:4.88E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPR014C(YPR014C_d)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOL120C6.379.61E-111.33RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YDL014W5.042.32E-70.18NOP1Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin
YCL054W4.865.95E-70.76SPB1AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
YBL026W4.102.07E-50.07LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YDR404C4.032.78E-50.36RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YOR146W_d3.671.20E-40.20YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YMR076C3.472.60E-40.02PDS5Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis
YKL210W3.452.82E-40.05UBA1Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress
YBR234C3.393.45E-40.02ARC40Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YGR083C3.383.66E-40.11GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YOR257W3.275.38E-40.17CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YHL015W3.109.64E-40.02RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
YER165W3.090.001010.09PAB1Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
YFR050C2.990.001390.01PRE4Beta 7 subunit of the 20S proteasome
YHR040W2.980.001440.12BCD1Essential protein required for the accumulation of box C/D snoRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR059W7.291.56E-13FYV4Protein of unknown function, required for survival upon exposure to K1 killer toxin
YJL037W6.523.44E-11IRC18Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci
YER118C5.629.42E-9SHO1Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p
YDL144C_p5.248.06E-8YDL144C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress
YOL059W4.953.62E-7GPD2NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1
YOR200W_d4.904.88E-7YOR200W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c
YGR287C4.425.02E-6IMA1Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family
YOR258W4.376.23E-6HNT3DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress
YPR046W4.289.31E-6MCM16Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3
YDL082W4.191.40E-5RPL13ARibosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication
YDR083W4.121.91E-5RRP8Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1
YKR094C4.111.95E-5RPL40BUbiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication
YDR202C4.092.11E-5RAV2Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme
YBR042C4.003.13E-5CST26Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication
YPL018W4.003.17E-5CTF19Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2

GO enrichment analysis for SGTC_1761
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0912.49E-12SGTC_1762st045503 25.5 μMTimTec (Natural product derivative library)46455540.115385
0.0699.23E-8SGTC_8003448-7362 155.0 μMChemDiv (Drug-like library)159919130.134021translation
0.0631.21E-6SGTC_620180-0423 3.2 μMChemDiv (Drug-like library)301020.094736860S ribosome export
0.0631.29E-6SGTC_32219128209 49.5 μMChembridge (Drug-like library)164607230.0816327
0.0621.56E-6SGTC_30079078383 71.4 μMChembridge (Drug-like library)64969950.115385
0.0621.71E-6SGTC_2654protoveratrine a 100.0 μMMicrosource (Natural product library)57022780.041958
0.0622.03E-6SGTC_1040k898-0338 155.0 μMChemDiv (Drug-like library)209693510.148148
0.0603.83E-6SGTC_1180083-0097 135.3 μMChemDiv (Drug-like library)52073560.0925926translation
0.0579.85E-6SGTC_31389097216 49.5 μMChembridge (Drug-like library)252374860.144444
0.0571.19E-5SGTC_2677mitomycin C 100.0 μMMiscellaneous57460.0865385DNA damage response
0.0561.68E-5SGTC_32479135149 49.5 μMChembridge (Drug-like library)89344360.0875DNA damage response
0.0552.56E-5SGTC_1037k284-1091 78.7 μMChemDiv (Drug-like library)33347110.11236
0.0543.24E-5SGTC_504lovastatin 124.0 μMICCB bioactive library532320.0701754
0.0534.97E-5SGTC_8324083-0019 164.0 μMChemDiv (Drug-like library)19907580.103774translation
0.0535.26E-5SGTC_1757st041620 31.8 μMTimTec (Natural product derivative library)52898150.0571429

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1730st03380845.1 μM0.6111111045166TimTec (Natural product derivative library)443.32583.77223
SGTC_6530203-001655.2 μM0.276316295302ChemDiv (Drug-like library)289.756743.44212
SGTC_2832799276251.95 μM0.2658232985177Chembridge (Drug-like library)299.71183.75204
SGTC_6681682-049962.6 μM0.2625682214ChemDiv (Drug-like library)324.20184.10612
SGTC_800159-0065512.24 μM0.2417581548079ChemDiv (Drug-like library)390.431784.49814
SGTC_13993562-577225 μM0.24137923847490ChemDiv (Drug-like library)394.7829834.86305RPP1 & pyrimidine depletion
SGTC_3253913598749.47 μM0.2272734182430Chembridge (Drug-like library)313.37421.4681460S ribosome export
SGTC_1704197-0004226 μM0.22619218692ChemDiv (Drug-like library)317.793283.72923
SGTC_2993905425771.43 μM0.2222226470217Chembridge (Drug-like library)343.850483.16113fatty acid desaturase (OLE1)
SGTC_2838900268771.43 μM0.220936457060Chembridge (Drug-like library)357.877063.64713fatty acid desaturase (OLE1)