st044815

methyl 3-amino-5-methoxy-1-benzofuran-2-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1765
Screen concentration 90.4 μM
Source TimTec (Natural product derivative library)
PubChem CID 645695
SMILES COC1=CC2=C(C=C1)OC(=C2N)C(=O)OC
Standardized SMILES COC(=O)c1oc2ccc(OC)cc2c1N
Molecular weight 221.2093
ALogP 1.52
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.55
% growth inhibition (Hom. pool) 6.47


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 645695
Download HIP data (tab-delimited text)  (excel)
Gene:DBP10(YDL031W)|FD-Score:-3.39|P-value:3.48E-4|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis Gene:GAA1(YLR088W)|FD-Score:3.29|P-value:4.93E-4|Clearance:0.13||SGD DESC:Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER Gene:GPI14(YJR013W)|FD-Score:4.57|P-value:2.42E-6|Clearance:0.14||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:MED6(YHR058C)|FD-Score:6.59|P-value:2.21E-11|Clearance:1.22||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:MIA40(YKL195W)|FD-Score:4.64|P-value:1.73E-6|Clearance:0.07||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:MTR3(YGR158C)|FD-Score:3.79|P-value:7.42E-5|Clearance:0.03||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NDC1(YML031W)|FD-Score:6.19|P-value:2.98E-10|Clearance:1.22||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NFS1(YCL017C)|FD-Score:-4.98|P-value:3.16E-7|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NUP192(YJL039C)|FD-Score:3.48|P-value:2.49E-4|Clearance:0.19||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:ORC3(YLL004W)|FD-Score:3.52|P-value:2.15E-4|Clearance:0.04||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PGS1(YCL004W)|FD-Score:-7.01|P-value:1.23E-12|Clearance:0||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRP24(YMR268C)|FD-Score:4.43|P-value:4.66E-6|Clearance:0.31||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RPB10(YOR210W)|FD-Score:3.76|P-value:8.42E-5|Clearance:0.24||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB8(YOR224C)|FD-Score:3.17|P-value:7.70E-4|Clearance:0.09||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPN3(YER021W)|FD-Score:4.12|P-value:1.87E-5|Clearance:0.03||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPT3(YDR394W)|FD-Score:4.09|P-value:2.14E-5|Clearance:0.04||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT4(YOR259C)|FD-Score:4.05|P-value:2.57E-5|Clearance:0.26||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RSC9(YML127W)|FD-Score:4.97|P-value:3.30E-7|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC26(YDR238C)|FD-Score:-3.32|P-value:4.54E-4|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SPP381(YBR152W)|FD-Score:4.92|P-value:4.24E-7|Clearance:0.28||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:TRS120(YDR407C)|FD-Score:-3.12|P-value:9.01E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:YOR218C(YOR218C_d)|FD-Score:-3.26|P-value:5.53E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W Gene:DBP10(YDL031W)|FD-Score:-3.39|P-value:3.48E-4|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis Gene:GAA1(YLR088W)|FD-Score:3.29|P-value:4.93E-4|Clearance:0.13||SGD DESC:Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER Gene:GPI14(YJR013W)|FD-Score:4.57|P-value:2.42E-6|Clearance:0.14||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:MED6(YHR058C)|FD-Score:6.59|P-value:2.21E-11|Clearance:1.22||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:MIA40(YKL195W)|FD-Score:4.64|P-value:1.73E-6|Clearance:0.07||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:MTR3(YGR158C)|FD-Score:3.79|P-value:7.42E-5|Clearance:0.03||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NDC1(YML031W)|FD-Score:6.19|P-value:2.98E-10|Clearance:1.22||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NFS1(YCL017C)|FD-Score:-4.98|P-value:3.16E-7|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NUP192(YJL039C)|FD-Score:3.48|P-value:2.49E-4|Clearance:0.19||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:ORC3(YLL004W)|FD-Score:3.52|P-value:2.15E-4|Clearance:0.04||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PGS1(YCL004W)|FD-Score:-7.01|P-value:1.23E-12|Clearance:0||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRP24(YMR268C)|FD-Score:4.43|P-value:4.66E-6|Clearance:0.31||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RPB10(YOR210W)|FD-Score:3.76|P-value:8.42E-5|Clearance:0.24||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB8(YOR224C)|FD-Score:3.17|P-value:7.70E-4|Clearance:0.09||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPN3(YER021W)|FD-Score:4.12|P-value:1.87E-5|Clearance:0.03||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPT3(YDR394W)|FD-Score:4.09|P-value:2.14E-5|Clearance:0.04||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT4(YOR259C)|FD-Score:4.05|P-value:2.57E-5|Clearance:0.26||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RSC9(YML127W)|FD-Score:4.97|P-value:3.30E-7|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC26(YDR238C)|FD-Score:-3.32|P-value:4.54E-4|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SPP381(YBR152W)|FD-Score:4.92|P-value:4.24E-7|Clearance:0.28||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:TRS120(YDR407C)|FD-Score:-3.12|P-value:9.01E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:YOR218C(YOR218C_d)|FD-Score:-3.26|P-value:5.53E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 645695
Download HOP data (tab-delimited text)  (excel)
Gene:ADK2(YER170W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:AEP2(YMR282C)|FD-Score:5.43|P-value:2.89E-8||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AHC2(YCR082W)|FD-Score:3.79|P-value:7.50E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ALD2(YMR170C)|FD-Score:-4.68|P-value:1.41E-6||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:APC9(YLR102C)|FD-Score:5.7|P-value:5.88E-9||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:APL5(YPL195W)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:ARL1(YBR164C)|FD-Score:5.44|P-value:2.63E-8||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARL3(YPL051W)|FD-Score:4.49|P-value:3.63E-6||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATG14(YBR128C)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor Gene:BOR1(YNL275W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:BUD2(YKL092C)|FD-Score:-4|P-value:3.19E-5||SGD DESC:GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint Gene:CIT1(YNR001C)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:COG8(YML071C)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DCC1(YCL016C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:EAR1(YMR171C)|FD-Score:-5.33|P-value:4.87E-8||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:EST2(YLR318W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:EXO5(YBR163W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:GET1(YGL020C)|FD-Score:8.58|P-value:4.67E-18||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GMH1(YKR030W)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting Gene:GTS1(YGL181W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:HPR1(YDR138W)|FD-Score:-3.18|P-value:7.36E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HTA1(YDR225W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:LRS4(YDR439W)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:LSC2(YGR244C)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MFM1(YPL060W)|FD-Score:3.85|P-value:5.85E-5||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MLP2(YIL149C)|FD-Score:6.22|P-value:2.55E-10||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:MNE1(YOR350C)|FD-Score:5.09|P-value:1.78E-7||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MRP49(YKL167C)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL10(YNL284C)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:MRPL13(YKR006C)|FD-Score:4.33|P-value:7.43E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPS12(YNR036C)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MTG1(YMR097C)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NPL3(YDR432W)|FD-Score:-4.09|P-value:2.20E-5||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PAD1(YDR538W)|FD-Score:4.57|P-value:2.48E-6||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PCP1(YGR101W)|FD-Score:3.86|P-value:5.71E-5||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PEP7(YDR323C)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:RAI1(YGL246C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RHO2(YNL090W)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RIC1(YLR039C)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL42B(YHR141C)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication Gene:RRP8(YDR083W)|FD-Score:4.33|P-value:7.42E-6||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:SKM1(YOL113W)|FD-Score:5.28|P-value:6.59E-8||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SLX5(YDL013W)|FD-Score:3.91|P-value:4.57E-5||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SNF3(YDL194W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication Gene:SRB8(YCR081W)|FD-Score:-3.89|P-value:4.94E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SRO77(YBL106C)|FD-Score:5|P-value:2.84E-7||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SYS1(YJL004C)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:THI72(YOR192C)|FD-Score:4.65|P-value:1.65E-6||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:TIP1(YBR067C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TLG2(YOL018C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP1(YDR007W)|FD-Score:3.83|P-value:6.50E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VPS1(YKR001C)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS24(YKL041W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS53(YJL029C)|FD-Score:-6.33|P-value:1.22E-10||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting Gene:VPS9(YML097C)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YBR113W(YBR113W_d)|FD-Score:5.61|P-value:9.83E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YBR200W-A(YBR200W-A_p)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR292C(YBR292C_d)|FD-Score:4.43|P-value:4.78E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Gene:YCL002C(YCL002C_p)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR099C(YCR099C_p)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function Gene:YDL026W(YDL026W_d)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER053C-A(YER053C-A_p)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YER076C(YER076C_p)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YKL069W(YKL069W)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YKL118W(YKL118W_d)|FD-Score:5.12|P-value:1.54E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLL006W-A(YLL006W-A_p)|FD-Score:-3.81|P-value:7.07E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR400W(YLR400W_d)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR160W(YMR160W_p)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YMR187C(YMR187C_p)|FD-Score:-3.78|P-value:7.71E-5||SGD DESC:Putative protein of unknown function; YMR187C is not an essential gene Gene:YMR316C-B(YMR316C-B_d)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL216W(YPL216W_p)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:ADK2(YER170W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:AEP2(YMR282C)|FD-Score:5.43|P-value:2.89E-8||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AHC2(YCR082W)|FD-Score:3.79|P-value:7.50E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ALD2(YMR170C)|FD-Score:-4.68|P-value:1.41E-6||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:APC9(YLR102C)|FD-Score:5.7|P-value:5.88E-9||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:APL5(YPL195W)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:ARL1(YBR164C)|FD-Score:5.44|P-value:2.63E-8||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARL3(YPL051W)|FD-Score:4.49|P-value:3.63E-6||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATG14(YBR128C)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor Gene:BOR1(YNL275W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:BUD2(YKL092C)|FD-Score:-4|P-value:3.19E-5||SGD DESC:GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint Gene:CIT1(YNR001C)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:COG8(YML071C)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DCC1(YCL016C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:EAR1(YMR171C)|FD-Score:-5.33|P-value:4.87E-8||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:EST2(YLR318W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:EXO5(YBR163W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:GET1(YGL020C)|FD-Score:8.58|P-value:4.67E-18||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GMH1(YKR030W)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting Gene:GTS1(YGL181W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:HPR1(YDR138W)|FD-Score:-3.18|P-value:7.36E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HTA1(YDR225W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:LRS4(YDR439W)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:LSC2(YGR244C)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MFM1(YPL060W)|FD-Score:3.85|P-value:5.85E-5||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MLP2(YIL149C)|FD-Score:6.22|P-value:2.55E-10||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:MNE1(YOR350C)|FD-Score:5.09|P-value:1.78E-7||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MRP49(YKL167C)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL10(YNL284C)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:MRPL13(YKR006C)|FD-Score:4.33|P-value:7.43E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPS12(YNR036C)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MTG1(YMR097C)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NPL3(YDR432W)|FD-Score:-4.09|P-value:2.20E-5||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PAD1(YDR538W)|FD-Score:4.57|P-value:2.48E-6||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PCP1(YGR101W)|FD-Score:3.86|P-value:5.71E-5||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PEP7(YDR323C)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:RAI1(YGL246C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RHO2(YNL090W)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RIC1(YLR039C)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL42B(YHR141C)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication Gene:RRP8(YDR083W)|FD-Score:4.33|P-value:7.42E-6||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:SKM1(YOL113W)|FD-Score:5.28|P-value:6.59E-8||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SLX5(YDL013W)|FD-Score:3.91|P-value:4.57E-5||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SNF3(YDL194W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication Gene:SRB8(YCR081W)|FD-Score:-3.89|P-value:4.94E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SRO77(YBL106C)|FD-Score:5|P-value:2.84E-7||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SYS1(YJL004C)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:THI72(YOR192C)|FD-Score:4.65|P-value:1.65E-6||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:TIP1(YBR067C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TLG2(YOL018C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP1(YDR007W)|FD-Score:3.83|P-value:6.50E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VPS1(YKR001C)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS24(YKL041W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS53(YJL029C)|FD-Score:-6.33|P-value:1.22E-10||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting Gene:VPS9(YML097C)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YBR113W(YBR113W_d)|FD-Score:5.61|P-value:9.83E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YBR200W-A(YBR200W-A_p)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR292C(YBR292C_d)|FD-Score:4.43|P-value:4.78E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Gene:YCL002C(YCL002C_p)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR099C(YCR099C_p)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function Gene:YDL026W(YDL026W_d)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER053C-A(YER053C-A_p)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YER076C(YER076C_p)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YKL069W(YKL069W)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YKL118W(YKL118W_d)|FD-Score:5.12|P-value:1.54E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLL006W-A(YLL006W-A_p)|FD-Score:-3.81|P-value:7.07E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR400W(YLR400W_d)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR160W(YMR160W_p)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YMR187C(YMR187C_p)|FD-Score:-3.78|P-value:7.71E-5||SGD DESC:Putative protein of unknown function; YMR187C is not an essential gene Gene:YMR316C-B(YMR316C-B_d)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL216W(YPL216W_p)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR058C6.592.21E-111.22MED6Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress
YML031W6.192.98E-101.22NDC1Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
YML127W4.973.30E-70.05RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YBR152W4.924.24E-70.28SPP381mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p
YKL195W4.641.73E-60.07MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YJR013W4.572.42E-60.14GPI14Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
YMR268C4.434.66E-60.31PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YER021W4.121.87E-50.03RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YDR394W4.092.14E-50.04RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YOR259C4.052.57E-50.26RPT4One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
YGR158C3.797.42E-50.03MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YOR210W3.768.42E-50.24RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YLL004W3.522.15E-40.04ORC3Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YJL039C3.482.49E-40.19NUP192Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205
YLR088W3.294.93E-40.13GAA1Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL020C8.584.67E-18GET1Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance
YIL149C6.222.55E-10MLP2Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length
YLR102C5.705.88E-9APC9Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YBR113W_d5.619.83E-9YBR113W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8
YBR164C5.442.63E-8ARL1Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor
YMR282C5.432.89E-8AEP2Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader
YOL113W5.286.59E-8SKM1Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth
YBR200W-A_p5.181.09E-7YBR200W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YKL118W_d5.121.54E-7YKL118W_dDubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2
YOR350C5.091.78E-7MNE1Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA
YBL106C5.002.84E-7SRO77Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication
YOR192C4.651.65E-6THI72Transporter of thiamine or related compound; shares sequence similarity with Thi7p
YDR538W4.572.48E-6PAD1Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX
YPL051W4.493.63E-6ARL3GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor
YBR292C_d4.434.78E-6YBR292C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene

GO enrichment analysis for SGTC_1765
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1403.39E-27SGTC_6051315-0399 119.0 μMChemDiv (Drug-like library)240204610.125Golgi
0.1322.37E-24SGTC_20415107442 45.3 μMChembridge (Fragment library)7930050.0909091RSC complex & mRNA processing
0.1316.84E-24SGTC_910417-1751 58.4 μMChemDiv (Drug-like library)67956240.0519481Golgi
0.1254.31E-22SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.047619Golgi
0.1233.32E-21SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.0598802Golgi
0.1102.28E-17SGTC_14083909-7976 48.8 μMChemDiv (Drug-like library)241523740.0533333Golgi
0.1087.29E-17SGTC_32779138724 49.5 μMChembridge (Drug-like library)159434180.0655738Golgi
0.1057.85E-16SGTC_930109-0045 59.2 μMChemDiv (Drug-like library)887330.150943DNA damage response
0.1041.40E-15SGTC_22426630449 61.3 μMChembridge (Fragment library)6912490.12069Golgi
0.0903.64E-12SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.0512821Golgi
0.0903.90E-12SGTC_28909046729 58.4 μMChembridge (Drug-like library)9033290.126984Golgi
0.0897.66E-12SGTC_21685727979 200.0 μMChembridge (Fragment library)6952100.0862069
0.0872.71E-11SGTC_1309pyrazolanthrone 13.4 μMChemDiv (Drug-like library)85150.0689655
0.0831.52E-10SGTC_33259138760 55.2 μMChembridge (Drug-like library)438449590.105263Golgi
0.0831.64E-10SGTC_1693xanthone 101.9 μMTimTec (Natural product derivative library)70200.106383DNA damage response

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2491paeonol100 μM0.33333311092Microsource (Natural product library)166.17391.31113
SGTC_5544483-2211187 μM0.326923747107ChemDiv (Drug-like library)285.29462.45314
SGTC_1759st04305970 μM0.314815669426TimTec (Natural product derivative library)285.29462.45314amide catabolism
SGTC_20474141938200 μM0.3043483807862Chembridge (Fragment library)169.1530231.14414
SGTC_3347918872144.72 μM0.344118411Chembridge (Drug-like library)263.719462.86923
SGTC_6304356-0227183 μM0.296296779312ChemDiv (Drug-like library)234.247862.61814
SGTC_7734358-145744.6 μM0.2950821186886ChemDiv (Drug-like library)352.38383.73716
SGTC_1615st00265686.5 μM0.2909091584458TimTec (Natural product derivative library)231.253821.1424redox potentiating
SGTC_22817943571200 μM0.2909091244024Chembridge (Fragment library)231.247222.47913
SGTC_15447-methoxy-4-methylcoumarin105 μM0.28390807TimTec (Pure natural product library)190.19532.32903