Deletion Strain | FD score | P-value | Clearance | Gene | Gene Description |
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YKL052C | 10.70 | 3.06E-27 | 1.51 | ASK1 | Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress |
YER133W | 10.00 | 5.32E-24 | 1.51 | GLC7 | Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest |
YER025W | 8.77 | 8.96E-19 | 1.51 | GCD11 | Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met |
YMR268C | 7.25 | 2.01E-13 | 0.95 | PRP24 | Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling |
YER048W-A | 6.30 | 1.48E-10 | 0.25 | ISD11 | Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis |
YNL126W | 6.05 | 7.07E-10 | 0.22 | SPC98 | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque |
YDL195W | 5.84 | 2.67E-9 | 0.39 | SEC31 | Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance |
YPL237W | 5.44 | 2.62E-8 | 1.08 | SUI3 | Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding |
YDR091C | 4.36 | 6.52E-6 | 0.05 | RLI1 | Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species |
YJL076W | 4.31 | 8.20E-6 | 0.11 | NET1 | Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication |
YGR083C | 4.19 | 1.37E-5 | 0.11 | GCD2 | Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression |
YJL010C | 4.08 | 2.21E-5 | 0.36 | NOP9 | Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain |
YPL238C_d | 3.73 | 9.68E-5 | 0.01 | YPL238C_d | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W |
YLR208W | 3.72 | 1.01E-4 | 0.13 | SEC13 | Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress |
YPR088C | 3.59 | 1.66E-4 | 0.06 | SRP54 | Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain |