st045414

6-chloro-3-hydroxy-2-pyridin-3-ylchromen-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1766
Screen concentration 44.8 μM
Source TimTec (Natural product derivative library)
PubChem CID 253960
SMILES C1=CC(=CN=C1)C2=C(C(=O)C3=C(O2)C=CC(=C3)Cl)O
Standardized SMILES OC1=C(Oc2ccc(Cl)cc2C1=O)c3cccnc3
Molecular weight 273.6712
ALogP 2.11
H-bond donor count 1
H-bond acceptor count 4
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 27.6
% growth inhibition (Hom. pool) 15.64


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 253960
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:-3.13|P-value:8.87E-4|Clearance:0||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ASK1(YKL052C)|FD-Score:10.7|P-value:3.06E-27|Clearance:1.51||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress Gene:CCT4(YDL143W)|FD-Score:-3.43|P-value:2.99E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:-4.75|P-value:1.02E-6|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC16(YKL022C)|FD-Score:-3.77|P-value:8.09E-5|Clearance:0||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:CYR1(YJL005W)|FD-Score:-3.17|P-value:7.55E-4|Clearance:0||SGD DESC:Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation Gene:EMG1(YLR186W)|FD-Score:3.39|P-value:3.46E-4|Clearance:0.02||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:GCD11(YER025W)|FD-Score:8.77|P-value:8.96E-19|Clearance:1.51||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GCD2(YGR083C)|FD-Score:4.19|P-value:1.37E-5|Clearance:0.11||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GLC7(YER133W)|FD-Score:10|P-value:5.32E-24|Clearance:1.51||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:ISD11(YER048W-A)|FD-Score:6.3|P-value:1.48E-10|Clearance:0.25||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:NET1(YJL076W)|FD-Score:4.31|P-value:8.20E-6|Clearance:0.11||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:NOP9(YJL010C)|FD-Score:4.08|P-value:2.21E-5|Clearance:0.36||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:PRP24(YMR268C)|FD-Score:7.25|P-value:2.01E-13|Clearance:0.95||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RLI1(YDR091C)|FD-Score:4.36|P-value:6.52E-6|Clearance:0.05||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPB8(YOR224C)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.07||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPO26(YPR187W)|FD-Score:-4.39|P-value:5.70E-6|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPP1(YHR062C)|FD-Score:3.52|P-value:2.12E-4|Clearance:0.13||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT1(YKL145W)|FD-Score:3.38|P-value:3.67E-4|Clearance:0.09||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:SEC13(YLR208W)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.13||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:5.84|P-value:2.67E-9|Clearance:0.39||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SPC98(YNL126W)|FD-Score:6.05|P-value:7.07E-10|Clearance:0.22||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SPT15(YER148W)|FD-Score:3.22|P-value:6.35E-4|Clearance:0.15||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:SRP54(YPR088C)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.06||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:SUI3(YPL237W)|FD-Score:5.44|P-value:2.62E-8|Clearance:1.08||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TOM40(YMR203W)|FD-Score:-3.16|P-value:7.85E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTP5(YDR398W)|FD-Score:-8.09|P-value:2.92E-16|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YPL238C(YPL238C_d)|FD-Score:3.73|P-value:9.68E-5|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:ACC1(YNR016C)|FD-Score:-3.13|P-value:8.87E-4|Clearance:0||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ASK1(YKL052C)|FD-Score:10.7|P-value:3.06E-27|Clearance:1.51||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress Gene:CCT4(YDL143W)|FD-Score:-3.43|P-value:2.99E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:-4.75|P-value:1.02E-6|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC16(YKL022C)|FD-Score:-3.77|P-value:8.09E-5|Clearance:0||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:CYR1(YJL005W)|FD-Score:-3.17|P-value:7.55E-4|Clearance:0||SGD DESC:Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation Gene:EMG1(YLR186W)|FD-Score:3.39|P-value:3.46E-4|Clearance:0.02||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:GCD11(YER025W)|FD-Score:8.77|P-value:8.96E-19|Clearance:1.51||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GCD2(YGR083C)|FD-Score:4.19|P-value:1.37E-5|Clearance:0.11||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GLC7(YER133W)|FD-Score:10|P-value:5.32E-24|Clearance:1.51||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:ISD11(YER048W-A)|FD-Score:6.3|P-value:1.48E-10|Clearance:0.25||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:NET1(YJL076W)|FD-Score:4.31|P-value:8.20E-6|Clearance:0.11||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:NOP9(YJL010C)|FD-Score:4.08|P-value:2.21E-5|Clearance:0.36||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:PRP24(YMR268C)|FD-Score:7.25|P-value:2.01E-13|Clearance:0.95||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RLI1(YDR091C)|FD-Score:4.36|P-value:6.52E-6|Clearance:0.05||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPB8(YOR224C)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.07||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPO26(YPR187W)|FD-Score:-4.39|P-value:5.70E-6|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPP1(YHR062C)|FD-Score:3.52|P-value:2.12E-4|Clearance:0.13||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT1(YKL145W)|FD-Score:3.38|P-value:3.67E-4|Clearance:0.09||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:SEC13(YLR208W)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.13||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:5.84|P-value:2.67E-9|Clearance:0.39||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SPC98(YNL126W)|FD-Score:6.05|P-value:7.07E-10|Clearance:0.22||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SPT15(YER148W)|FD-Score:3.22|P-value:6.35E-4|Clearance:0.15||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:SRP54(YPR088C)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.06||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:SUI3(YPL237W)|FD-Score:5.44|P-value:2.62E-8|Clearance:1.08||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TOM40(YMR203W)|FD-Score:-3.16|P-value:7.85E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTP5(YDR398W)|FD-Score:-8.09|P-value:2.92E-16|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YPL238C(YPL238C_d)|FD-Score:3.73|P-value:9.68E-5|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 253960
Download HOP data (tab-delimited text)  (excel)
Gene:ABP1(YCR088W)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences Gene:ADE1(YAR015W)|FD-Score:3.72|P-value:9.78E-5||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:ADE3(YGR204W)|FD-Score:-4|P-value:3.14E-5||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:ASN2(YGR124W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:BCK1(YJL095W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CCR4(YAL021C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CHA1(YCL064C)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:CST6(YIL036W)|FD-Score:4.28|P-value:9.50E-6||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:CTT1(YGR088W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Cytosolic catalase T, has a role in protection from oxidative damage by hydrogen peroxide Gene:DAL81(YIR023W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DEG1(YFL001W)|FD-Score:3.5|P-value:2.28E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DID2(YKR035W-A)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors Gene:ECM18(YDR125C)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:ECM32(YER176W)|FD-Score:3.76|P-value:8.41E-5||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:ENV10(YLR065C)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ENV7(YPL236C)|FD-Score:5.44|P-value:2.74E-8||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:ERG3(YLR056W)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FMP21(YBR269C_p)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP46(YKR049C)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE7(YOL152W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:GAL7(YBR018C)|FD-Score:6.07|P-value:6.23E-10||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GCR2(YNL199C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GGA2(YHR108W)|FD-Score:6.94|P-value:1.91E-12||SGD DESC:Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin Gene:GND1(YHR183W)|FD-Score:-4.73|P-value:1.13E-6||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:GTR2(YGR163W)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HCR1(YLR192C)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HUR1(YGL168W)|FD-Score:5.17|P-value:1.18E-7||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:ICY2(YPL250C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:IPK1(YDR315C)|FD-Score:-3.89|P-value:5.03E-5||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:LDB19(YOR322C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LEM3(YNL323W)|FD-Score:-4.81|P-value:7.66E-7||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MET16(YPR167C)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism Gene:MFM1(YPL060W)|FD-Score:4|P-value:3.23E-5||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MIP6(YHR015W)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication Gene:MNL1(YHR204W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MPA43(YNL249C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NPT1(YOR209C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OCA2(YNL056W)|FD-Score:-6.98|P-value:1.43E-12||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:PEP3(YLR148W)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PFK2(YMR205C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PGD1(YGL025C)|FD-Score:7.95|P-value:9.14E-16||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PMR1(YGL167C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:PTK2(YJR059W)|FD-Score:-3.75|P-value:8.94E-5||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUG1(YER185W)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:RAX2(YLR084C)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RCY1(YJL204C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RDS1(YCR106W)|FD-Score:-4.14|P-value:1.73E-5||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:REG1(YDR028C)|FD-Score:5.21|P-value:9.34E-8||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIC1(YLR039C)|FD-Score:4.88|P-value:5.25E-7||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPE1(YJL121C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL42B(YHR141C)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication Gene:RRT12(YCR045C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RTG1(YOL067C)|FD-Score:4|P-value:3.14E-5||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTN2(YDL204W)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:7.76|P-value:4.21E-15||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDS3(YIL084C)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SHE4(YOR035C)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SHP1(YBL058W)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SIN4(YNL236W)|FD-Score:19.7|P-value:6.09E-87||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SIP3(YNL257C)|FD-Score:3.85|P-value:6.00E-5||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SLG1(YOR008C)|FD-Score:5.33|P-value:4.95E-8||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNQ2(YDR011W)|FD-Score:6.61|P-value:1.97E-11||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SOL4(YGR248W)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SOV1(YMR066W)|FD-Score:3.76|P-value:8.58E-5||SGD DESC:Mitochondrial protein of unknown function Gene:SRO77(YBL106C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SSN2(YDR443C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:TAE2(YPL009C)|FD-Score:-3.83|P-value:6.44E-5||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TDA8(YAL064C-A_p)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TPS3(YMR261C)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:TRP3(YKL211C)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRS65(YGR166W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:TYW1(YPL207W)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:VMA8(YEL051W)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS21(YOR089C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS45(YGL095C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS5(YOR069W)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:XRS2(YDR369C)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBR141C(YBR141C_p)|FD-Score:-7.27|P-value:1.79E-13||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YBR300C(YBR300C_d)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YDL041W(YDL041W_d)|FD-Score:3.75|P-value:8.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YER134C(YER134C)|FD-Score:4.69|P-value:1.36E-6||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YKL053W(YKL053W_d)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YKL091C(YKL091C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YKR005C(YKR005C_p)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Putative protein of unknown function Gene:YKU70(YMR284W)|FD-Score:-4.85|P-value:6.25E-7||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YML012C-A(YML012C-A_d)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR099C(YMR099C)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YMR102C(YMR102C_p)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YMR111C(YMR111C_p)|FD-Score:-3.15|P-value:8.24E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress Gene:YNL198C(YNL198C_d)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL235C(YNL235C_d)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YPR174C(YPR174C_p)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; relative distribution to foci at the nuclear periphery increases upon DNA replication stress; YPR174C has a paralog, NBP1, that arose from the whole genome duplication Gene:ABP1(YCR088W)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences Gene:ADE1(YAR015W)|FD-Score:3.72|P-value:9.78E-5||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:ADE3(YGR204W)|FD-Score:-4|P-value:3.14E-5||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:ASN2(YGR124W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:BCK1(YJL095W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CCR4(YAL021C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CHA1(YCL064C)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:CST6(YIL036W)|FD-Score:4.28|P-value:9.50E-6||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:CTT1(YGR088W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Cytosolic catalase T, has a role in protection from oxidative damage by hydrogen peroxide Gene:DAL81(YIR023W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DEG1(YFL001W)|FD-Score:3.5|P-value:2.28E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DID2(YKR035W-A)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors Gene:ECM18(YDR125C)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:ECM32(YER176W)|FD-Score:3.76|P-value:8.41E-5||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:ENV10(YLR065C)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ENV7(YPL236C)|FD-Score:5.44|P-value:2.74E-8||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:ERG3(YLR056W)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FMP21(YBR269C_p)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP46(YKR049C)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE7(YOL152W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:GAL7(YBR018C)|FD-Score:6.07|P-value:6.23E-10||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GCR2(YNL199C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GGA2(YHR108W)|FD-Score:6.94|P-value:1.91E-12||SGD DESC:Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin Gene:GND1(YHR183W)|FD-Score:-4.73|P-value:1.13E-6||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:GTR2(YGR163W)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HCR1(YLR192C)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HUR1(YGL168W)|FD-Score:5.17|P-value:1.18E-7||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:ICY2(YPL250C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:IPK1(YDR315C)|FD-Score:-3.89|P-value:5.03E-5||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:LDB19(YOR322C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LEM3(YNL323W)|FD-Score:-4.81|P-value:7.66E-7||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MET16(YPR167C)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism Gene:MFM1(YPL060W)|FD-Score:4|P-value:3.23E-5||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MIP6(YHR015W)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication Gene:MNL1(YHR204W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MPA43(YNL249C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NPT1(YOR209C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OCA2(YNL056W)|FD-Score:-6.98|P-value:1.43E-12||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:PEP3(YLR148W)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PFK2(YMR205C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PGD1(YGL025C)|FD-Score:7.95|P-value:9.14E-16||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PMR1(YGL167C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:PTK2(YJR059W)|FD-Score:-3.75|P-value:8.94E-5||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUG1(YER185W)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:RAX2(YLR084C)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RCY1(YJL204C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RDS1(YCR106W)|FD-Score:-4.14|P-value:1.73E-5||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:REG1(YDR028C)|FD-Score:5.21|P-value:9.34E-8||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIC1(YLR039C)|FD-Score:4.88|P-value:5.25E-7||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPE1(YJL121C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL42B(YHR141C)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication Gene:RRT12(YCR045C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RTG1(YOL067C)|FD-Score:4|P-value:3.14E-5||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTN2(YDL204W)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:7.76|P-value:4.21E-15||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDS3(YIL084C)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SHE4(YOR035C)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SHP1(YBL058W)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SIN4(YNL236W)|FD-Score:19.7|P-value:6.09E-87||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SIP3(YNL257C)|FD-Score:3.85|P-value:6.00E-5||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SLG1(YOR008C)|FD-Score:5.33|P-value:4.95E-8||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNQ2(YDR011W)|FD-Score:6.61|P-value:1.97E-11||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SOL4(YGR248W)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SOV1(YMR066W)|FD-Score:3.76|P-value:8.58E-5||SGD DESC:Mitochondrial protein of unknown function Gene:SRO77(YBL106C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SSN2(YDR443C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:TAE2(YPL009C)|FD-Score:-3.83|P-value:6.44E-5||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TDA8(YAL064C-A_p)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TPS3(YMR261C)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:TRP3(YKL211C)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRS65(YGR166W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:TYW1(YPL207W)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:VMA8(YEL051W)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS21(YOR089C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS45(YGL095C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS5(YOR069W)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:XRS2(YDR369C)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBR141C(YBR141C_p)|FD-Score:-7.27|P-value:1.79E-13||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YBR300C(YBR300C_d)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YDL041W(YDL041W_d)|FD-Score:3.75|P-value:8.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YER134C(YER134C)|FD-Score:4.69|P-value:1.36E-6||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YKL053W(YKL053W_d)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YKL091C(YKL091C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YKR005C(YKR005C_p)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Putative protein of unknown function Gene:YKU70(YMR284W)|FD-Score:-4.85|P-value:6.25E-7||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YML012C-A(YML012C-A_d)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR099C(YMR099C)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YMR102C(YMR102C_p)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YMR111C(YMR111C_p)|FD-Score:-3.15|P-value:8.24E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress Gene:YNL198C(YNL198C_d)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL235C(YNL235C_d)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YPR174C(YPR174C_p)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; relative distribution to foci at the nuclear periphery increases upon DNA replication stress; YPR174C has a paralog, NBP1, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL052C10.703.06E-271.51ASK1Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
YER133W10.005.32E-241.51GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YER025W8.778.96E-191.51GCD11Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met
YMR268C7.252.01E-130.95PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YER048W-A6.301.48E-100.25ISD11Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis
YNL126W6.057.07E-100.22SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YDL195W5.842.67E-90.39SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YPL237W5.442.62E-81.08SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YDR091C4.366.52E-60.05RLI1Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species
YJL076W4.318.20E-60.11NET1Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication
YGR083C4.191.37E-50.11GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YJL010C4.082.21E-50.36NOP9Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
YPL238C_d3.739.68E-50.01YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YLR208W3.721.01E-40.13SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YPR088C3.591.66E-40.06SRP54Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL236W19.706.09E-87SIN4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
YGL025C7.959.14E-16PGD1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
YDR129C7.764.21E-15SAC6Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YHR108W6.941.91E-12GGA2Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
YDR011W6.611.97E-11SNQ2Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
YBR018C6.076.23E-10GAL7Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism
YPL236C5.442.74E-8ENV7Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)
YOR008C5.334.95E-8SLG1Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
YDR028C5.219.34E-8REG1Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p
YGL168W5.171.18E-7HUR1Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene
YOR069W5.091.82E-7VPS5Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes
YLR039C4.885.25E-7RIC1Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes
YDR125C4.731.14E-6ECM18Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication
YER134C4.691.36E-6YER134CMagnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene
YIL036W4.289.50E-6CST6Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1766
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2881.13E-112SGTC_13993562-5772 25.0 μMChemDiv (Drug-like library)238474900.178082RPP1 & pyrimidine depletion
0.2811.71E-107SGTC_1959st077091 55.8 μMTimTec (Natural product derivative library)8213560.184615RPP1 & pyrimidine depletion
0.2701.16E-98SGTC_21565649887 115.0 μMChembridge (Fragment library)54680440NARPP1 & pyrimidine depletion
0.2251.67E-68SGTC_32329132874 49.5 μMChembridge (Drug-like library)37849400.0923077RPP1 & pyrimidine depletion
0.2244.26E-68SGTC_6001495-0418 41.6 μMChemDiv (Drug-like library)53777680.0735294RPP1 & pyrimidine depletion
0.2053.42E-57SGTC_2509methyl 7-deshydroxypyrogallin-4-carboxylate 54.6 μMMicrosource (Natural product library)50988840.119403RPP1 & pyrimidine depletion
0.2004.71E-54SGTC_28107959871 45.5 μMChembridge (Drug-like library)29745350.0864198copper-dependent oxidative stress
0.1831.76E-45SGTC_2507purpurin 79.7 μMMicrosource (Natural product library)66830.207547copper-dependent oxidative stress
0.1771.03E-42SGTC_20575236571 38.3 μMChembridge (Fragment library)54684039NARPP1 & pyrimidine depletion
0.1672.46E-38SGTC_1834st055981 52.8 μMTimTec (Natural product derivative library)4717190.21875RPP1 & pyrimidine depletion
0.1582.10E-34SGTC_3181488-0629 67.2 μMChemDiv (Drug-like library)36625760.0821918copper-dependent oxidative stress
0.1562.35E-33SGTC_28057950783 58.4 μMChembridge (Drug-like library)11789580.313433
0.1529.41E-32SGTC_29269010726 65.2 μMChembridge (Drug-like library)29942930.084507
0.1452.89E-29SGTC_7251497-0963 19.0 μMChemDiv (Drug-like library)35499770.153846copper-dependent oxidative stress
0.1432.35E-28SGTC_32009114385 49.5 μMChembridge (Drug-like library)170180300.0857143

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1780st03844824.54 μM0.56728868TimTec (Natural product derivative library)278.710883.21614iron homeostasis
SGTC_15823',3',6'-trihydroxyflavone74 μM0.520833688803TimTec (Pure natural product library)270.23692.11435
SGTC_1109tnp00067928.26 nM0.46676295TimTec (Natural product library)254.23752.35624
SGTC_1112tnp000691.17 μM0.431373145826TimTec (Natural product library)270.23692.11435
SGTC_11103-hydroxy-3'-methoxyflavone1.24 μM0.418182676296TimTec (Natural product library)268.264082.58214iron homeostasis
SGTC_15494'-methoxyflavonol74.6 μM0.38888997141TimTec (Pure natural product library)268.264082.58214
SGTC_1830st05598250.3 μM0.387755455313TimTec (Natural product derivative library)254.23752.35624
SGTC_1115galangin695.68 nM0.3773585281616TimTec (Natural product library)270.23692.11435RPP1 & pyrimidine depletion
SGTC_2505galangin7.89 μM0.3773585281616Microsource (Natural product library)270.23692.11435RPP1 & pyrimidine depletion
SGTC_1124fisetin1.16 μM0.370375281614TimTec (Natural product library)286.23631.87246
SGTC_1723st0343072.55 μM0.346154867466TimTec (Natural product derivative library)297.949563.81402mitochondrial processes