st044992

(13S,17E)-17-hydrazinylidene-13-methyl-7,8,9,11,12,14,15,16-octahydro-6H-cyclopenta[a]phenanthren-3-ol

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1767
Screen concentration 70.3 μM
Source TimTec (Natural product derivative library)
PubChem CID 9600693
SMILES CC12CCC3C(C1CCC2=NN)CCC4=C3C=CC(=C4)O
Standardized SMILES CC12CCC3C(CCc4cc(O)ccc34)C1CCC2=NN
Molecular weight 284.396
ALogP 3.5
H-bond donor count 2
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.66
% growth inhibition (Hom. pool) 4.3


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 9600693
Download HIP data (tab-delimited text)  (excel)
Gene:ADE13(YLR359W)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.1||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:ARC19(YKL013C)|FD-Score:3.14|P-value:8.47E-4|Clearance:0.01||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:DED81(YHR019C)|FD-Score:-3.13|P-value:8.73E-4|Clearance:0||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:ERD2(YBL040C)|FD-Score:-4.22|P-value:1.22E-5|Clearance:0||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:ERG1(YGR175C)|FD-Score:3.32|P-value:4.47E-4|Clearance:0.09||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:FAP7(YDL166C)|FD-Score:3.13|P-value:8.70E-4|Clearance:0.23||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:FRQ1(YDR373W)|FD-Score:3.61|P-value:1.52E-4|Clearance:0.15||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:IQG1(YPL242C)|FD-Score:3.92|P-value:4.47E-5|Clearance:0.31||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ISD11(YER048W-A)|FD-Score:-3.23|P-value:6.28E-4|Clearance:0||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:KOG1(YHR186C)|FD-Score:-3.5|P-value:2.35E-4|Clearance:0||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:MCM10(YIL150C)|FD-Score:4.03|P-value:2.82E-5|Clearance:0.11||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MCM2(YBL023C)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.11||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:NUP82(YJL061W)|FD-Score:3.35|P-value:3.97E-4|Clearance:0.03||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:RPN12(YFR052W)|FD-Score:-3.72|P-value:9.95E-5|Clearance:0||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress Gene:SNU114(YKL173W)|FD-Score:-4.35|P-value:6.92E-6|Clearance:0||SGD DESC:GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 Gene:STT4(YLR305C)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization Gene:TIF6(YPR016C)|FD-Score:4.46|P-value:4.09E-6|Clearance:0.43||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:WRS1(YOL097C)|FD-Score:-3.27|P-value:5.42E-4|Clearance:0||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YAL034C-B(YAL034C-B_d)|FD-Score:-3.21|P-value:6.71E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR023C(YJR023C_d)|FD-Score:-3.47|P-value:2.59E-4|Clearance:0||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing Gene:YNL247W(YNL247W)|FD-Score:-3.18|P-value:7.24E-4|Clearance:0||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments Gene:ADE13(YLR359W)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.1||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:ARC19(YKL013C)|FD-Score:3.14|P-value:8.47E-4|Clearance:0.01||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:DED81(YHR019C)|FD-Score:-3.13|P-value:8.73E-4|Clearance:0||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:ERD2(YBL040C)|FD-Score:-4.22|P-value:1.22E-5|Clearance:0||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:ERG1(YGR175C)|FD-Score:3.32|P-value:4.47E-4|Clearance:0.09||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:FAP7(YDL166C)|FD-Score:3.13|P-value:8.70E-4|Clearance:0.23||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:FRQ1(YDR373W)|FD-Score:3.61|P-value:1.52E-4|Clearance:0.15||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:IQG1(YPL242C)|FD-Score:3.92|P-value:4.47E-5|Clearance:0.31||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ISD11(YER048W-A)|FD-Score:-3.23|P-value:6.28E-4|Clearance:0||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:KOG1(YHR186C)|FD-Score:-3.5|P-value:2.35E-4|Clearance:0||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:MCM10(YIL150C)|FD-Score:4.03|P-value:2.82E-5|Clearance:0.11||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MCM2(YBL023C)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.11||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:NUP82(YJL061W)|FD-Score:3.35|P-value:3.97E-4|Clearance:0.03||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:RPN12(YFR052W)|FD-Score:-3.72|P-value:9.95E-5|Clearance:0||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress Gene:SNU114(YKL173W)|FD-Score:-4.35|P-value:6.92E-6|Clearance:0||SGD DESC:GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 Gene:STT4(YLR305C)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization Gene:TIF6(YPR016C)|FD-Score:4.46|P-value:4.09E-6|Clearance:0.43||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:WRS1(YOL097C)|FD-Score:-3.27|P-value:5.42E-4|Clearance:0||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YAL034C-B(YAL034C-B_d)|FD-Score:-3.21|P-value:6.71E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR023C(YJR023C_d)|FD-Score:-3.47|P-value:2.59E-4|Clearance:0||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing Gene:YNL247W(YNL247W)|FD-Score:-3.18|P-value:7.24E-4|Clearance:0||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 9600693
Download HOP data (tab-delimited text)  (excel)
Gene:ALD2(YMR170C)|FD-Score:-3.79|P-value:7.49E-5||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ASG7(YJL170C)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor Gene:ATP1(YBL099W)|FD-Score:3.19|P-value:6.99E-4||SGD DESC:Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BUD2(YKL092C)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint Gene:CAJ1(YER048C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CKB1(YGL019W)|FD-Score:-3.76|P-value:8.58E-5||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:COG5(YNL051W)|FD-Score:4.31|P-value:8.01E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CST6(YIL036W)|FD-Score:7.4|P-value:6.79E-14||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:CUE1(YMR264W)|FD-Score:5.18|P-value:1.12E-7||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DFG5(YMR238W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:ENV7(YPL236C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:ENV9(YOR246C)|FD-Score:6.12|P-value:4.66E-10||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:FAB1(YFR019W)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FIR1(YER032W)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:FRE3(YOR381W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:GMC1(YDR506C)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:HST1(YOL068C)|FD-Score:-4.3|P-value:8.57E-6||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:IMD3(YLR432W)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:JNM1(YMR294W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KGD2(YDR148C)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated Gene:MAD2(YJL030W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MAK10(YEL053C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MDH2(YOL126C)|FD-Score:5.46|P-value:2.38E-8||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MDJ1(YFL016C)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MEK1(YOR351C)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids Gene:MET17(YLR303W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MRM1(YOR201C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MSA2(YKR077W)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MST1(YKL194C)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NRP1(YDL167C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:OCA1(YNL099C)|FD-Score:4.76|P-value:9.56E-7||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OCA6(YDR067C_p)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:OSH3(YHR073W)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PET111(YMR257C)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PEX2(YJL210W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:RING-finger peroxin and E3 ubiquitin ligase, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import Gene:PHO90(YJL198W)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:PRK1(YIL095W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:PRY2(YKR013W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:PTH2(YBL057C)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:RFM1(YOR279C)|FD-Score:4.01|P-value:3.00E-5||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RMD8(YFR048W)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RPL21B(YPL079W)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPS11B(YBR048W)|FD-Score:3.74|P-value:9.05E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RPS1A(YLR441C)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RTR1(YER139C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:SFL1(YOR140W)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SFM1(YOR021C)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SPO22(YIL073C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:SYH1(YPL105C)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TPS3(YMR261C)|FD-Score:-4.17|P-value:1.54E-5||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:UGA2(YBR006W)|FD-Score:9.59|P-value:4.30E-22||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VPS4(YPR173C)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:YCR016W(YCR016W_p)|FD-Score:-3.1|P-value:9.73E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YDR467C(YDR467C_d)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL043W(YEL043W)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YGK3(YOL128C)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGR130C(YGR130C)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YGR219W(YGR219W_d)|FD-Score:-4.56|P-value:2.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YHL005C(YHL005C_d)|FD-Score:5.16|P-value:1.25E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W Gene:YHR125W(YHR125W_d)|FD-Score:4.31|P-value:8.00E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL107C(YJL107C_p)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi Gene:YJR015W(YJR015W_p)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YLR001C(YLR001C_p)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR294C(YLR294C_d)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Gene:YMR306C-A(YMR306C-A_d)|FD-Score:5.33|P-value:4.81E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR065C(YNR065C_p)|FD-Score:4.85|P-value:6.19E-7||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR225W(YOR225W_d)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR277C(YOR277C_d)|FD-Score:4.41|P-value:5.26E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YPL182C(YPL182C_d)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPQ1(YOL092W_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:ALD2(YMR170C)|FD-Score:-3.79|P-value:7.49E-5||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ASG7(YJL170C)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor Gene:ATP1(YBL099W)|FD-Score:3.19|P-value:6.99E-4||SGD DESC:Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BUD2(YKL092C)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint Gene:CAJ1(YER048C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CKB1(YGL019W)|FD-Score:-3.76|P-value:8.58E-5||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:COG5(YNL051W)|FD-Score:4.31|P-value:8.01E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CST6(YIL036W)|FD-Score:7.4|P-value:6.79E-14||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:CUE1(YMR264W)|FD-Score:5.18|P-value:1.12E-7||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DFG5(YMR238W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:ENV7(YPL236C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:ENV9(YOR246C)|FD-Score:6.12|P-value:4.66E-10||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:FAB1(YFR019W)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FIR1(YER032W)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:FRE3(YOR381W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:GMC1(YDR506C)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:HST1(YOL068C)|FD-Score:-4.3|P-value:8.57E-6||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:IMD3(YLR432W)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:JNM1(YMR294W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KGD2(YDR148C)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated Gene:MAD2(YJL030W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MAK10(YEL053C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MDH2(YOL126C)|FD-Score:5.46|P-value:2.38E-8||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MDJ1(YFL016C)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MEK1(YOR351C)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids Gene:MET17(YLR303W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MRM1(YOR201C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MSA2(YKR077W)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MST1(YKL194C)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NRP1(YDL167C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:OCA1(YNL099C)|FD-Score:4.76|P-value:9.56E-7||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OCA6(YDR067C_p)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:OSH3(YHR073W)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PET111(YMR257C)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PEX2(YJL210W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:RING-finger peroxin and E3 ubiquitin ligase, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import Gene:PHO90(YJL198W)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:PRK1(YIL095W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:PRY2(YKR013W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:PTH2(YBL057C)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:RFM1(YOR279C)|FD-Score:4.01|P-value:3.00E-5||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RMD8(YFR048W)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RPL21B(YPL079W)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPS11B(YBR048W)|FD-Score:3.74|P-value:9.05E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RPS1A(YLR441C)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RTR1(YER139C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:SFL1(YOR140W)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SFM1(YOR021C)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SPO22(YIL073C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:SYH1(YPL105C)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TPS3(YMR261C)|FD-Score:-4.17|P-value:1.54E-5||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:UGA2(YBR006W)|FD-Score:9.59|P-value:4.30E-22||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VPS4(YPR173C)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:YCR016W(YCR016W_p)|FD-Score:-3.1|P-value:9.73E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YDR467C(YDR467C_d)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL043W(YEL043W)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YGK3(YOL128C)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGR130C(YGR130C)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YGR219W(YGR219W_d)|FD-Score:-4.56|P-value:2.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YHL005C(YHL005C_d)|FD-Score:5.16|P-value:1.25E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W Gene:YHR125W(YHR125W_d)|FD-Score:4.31|P-value:8.00E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL107C(YJL107C_p)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi Gene:YJR015W(YJR015W_p)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YLR001C(YLR001C_p)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR294C(YLR294C_d)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Gene:YMR306C-A(YMR306C-A_d)|FD-Score:5.33|P-value:4.81E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR065C(YNR065C_p)|FD-Score:4.85|P-value:6.19E-7||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR225W(YOR225W_d)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR277C(YOR277C_d)|FD-Score:4.41|P-value:5.26E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YPL182C(YPL182C_d)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPQ1(YOL092W_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR016C4.464.09E-60.43TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YIL150C4.032.82E-50.11MCM10Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
YPL242C3.924.47E-50.30IQG1Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
YDR373W3.611.52E-40.15FRQ1N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
YBL023C3.462.67E-40.11MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YJL061W3.353.97E-40.03NUP82Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p)
YGR175C3.324.47E-40.09ERG1Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
YLR359W3.246.07E-40.10ADE13Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
YKL013C3.148.47E-40.01ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YDL166C3.138.70E-40.23FAP7Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D
YLR197W2.900.001870.12NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YDR407C2.780.002730.10TRS120One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YGR098C2.680.003712.09E-4ESP1Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
YER147C2.680.003710.00SCC4Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX
YOR257W2.680.003730.01CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR006W9.594.30E-22UGA2Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
YIL036W7.406.79E-14CST6Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication
YOR246C6.124.66E-10ENV9Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
YOL126C5.462.38E-8MDH2Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1
YMR306C-A_d5.334.81E-8YMR306C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YMR264W5.181.12E-7CUE1Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication
YHL005C_d5.161.25E-7YHL005C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W
YNR065C_p4.856.19E-7YNR065C_pProtein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene
YNL099C4.769.56E-7OCA1Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA
YKR077W4.741.09E-6MSA2Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation
YJL170C4.721.19E-6ASG7Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor
YOR140W4.592.22E-6SFL1Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p
YOR277C_d4.415.26E-6YOR277C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20
YHR125W_d4.318.00E-6YHR125W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YNL051W4.318.01E-6COG5Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments

GO enrichment analysis for SGTC_1767
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0758.31E-9SGTC_2094077-0173 175.6 μMChemDiv (Drug-like library)159934940.0909091
0.0672.42E-7SGTC_30909117696 49.5 μMChembridge (Drug-like library)79238210.0666667
0.0672.90E-7SGTC_2502avocadyne acetate 18.6 μMMicrosource (Natural product library)39520790.0263158
0.0654.76E-7SGTC_29679089538 49.9 μMChembridge (Drug-like library)7282160.05
0.0631.09E-6SGTC_13923455-1037 33.9 μMChemDiv (Drug-like library)9811320.0804598
0.0631.16E-6SGTC_15024534-4282 27.7 μMChemDiv (Drug-like library)32789490.0617284
0.0612.74E-6SGTC_1647st011722 22.4 μMTimTec (Natural product derivative library)28547830.140845
0.0603.73E-6SGTC_28057950783 58.4 μMChembridge (Drug-like library)11789580.0337079
0.0596.03E-6SGTC_1678st019265 80.2 μMTimTec (Natural product derivative library)53670200.041666760S ribosome export
0.0588.38E-6SGTC_2413inermin 26.1 μMTimTec (Natural product derivative library)915100.212121
0.0552.75E-5SGTC_10104112-3296 15.2 μMChemDiv (Drug-like library)7043840.0512821
0.0526.77E-5SGTC_1568digitonin 820.0 nMTimTec (Pure natural product library)27350100.0833333sphingolipid biosynthesis & PDR1
0.0501.21E-4SGTC_14023909-7673 35.9 μMChemDiv (Drug-like library)53280140.0405405
0.0501.27E-4SGTC_21055350118 200.0 μMChembridge (Fragment library)21333130.0540541
0.0501.36E-4SGTC_31799107801 49.5 μMChembridge (Drug-like library)48182590.0864198

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_406estrone100 μM0.75870Miscellaneous270.366083.93812
SGTC_1519esterone74 μM0.7698TimTec (Pure natural product library)270.366083.93812DNA intercalators
SGTC_231b-estradiol524 μM0.584906450Miscellaneous272.381963.83822
SGTC_1587estradiol valerate56.1 μM0.45588213791TimTec (Pure natural product library)356.498385.79613
SGTC_416estradiol valerate100 μM0.45588213791TimTec (Pure natural product library)356.498385.79613
SGTC_417st075190100 μM0.366197222757TimTec (Pure natural product library)376.488025.51213
SGTC_2678equilin100 μM0.3593753247Microsource (Natural product library)268.35023.68812
SGTC_25303-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene92.62 μM0.2622956708760Microsource (Natural product library)216.31873.35611
SGTC_2650prasterone100 μM0.26153816759247Microsource (Natural product library)288.424423.33812
SGTC_2758dehydroepiandrosterone69.35 μM0.2615385881Miscellaneous288.424423.33812
SGTC_1572epiandrosterone68.9 μM0.242424441302TimTec (Pure natural product library)290.44033.58812