st044301

3-[2-(1,2-dimethylindol-3-yl)ethylamino]-1-(4-propoxyphenyl)pyrrolidine-2,5-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1768
Screen concentration 37.2 μM
Source TimTec (Natural product derivative library)
PubChem CID 3931349
SMILES CCCOC1=CC=C(C=C1)N2C(=O)CC(C2=O)NCCC3=C(N(C4=CC=CC=C43)C)C
Standardized SMILES CCCOc1ccc(cc1)N2C(=O)CC(NCCc3c(C)n(C)c4ccccc34)C2=O
Molecular weight 419.5161
ALogP 3.89
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 2.94
% growth inhibition (Hom. pool) 4.3


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3931349
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:-3.63|P-value:1.44E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ASA1(YPR085C)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.08||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CLP1(YOR250C)|FD-Score:4.57|P-value:2.49E-6|Clearance:0.13||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:GCD2(YGR083C)|FD-Score:4.86|P-value:5.73E-7|Clearance:0.3||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI18(YBR004C)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.06||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:KRE5(YOR336W)|FD-Score:4.31|P-value:8.21E-6|Clearance:0.07||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:NAM9(YNL137C)|FD-Score:3.1|P-value:9.52E-4|Clearance:0.01||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:NAT2(YGR147C)|FD-Score:3.55|P-value:1.93E-4|Clearance:0.01||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NOG2(YNR053C)|FD-Score:3.38|P-value:3.59E-4|Clearance:0.19||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:RPC53(YDL150W)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.13||SGD DESC:RNA polymerase III subunit C53 Gene:RRP4(YHR069C)|FD-Score:4.24|P-value:1.13E-5|Clearance:0.29||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC65(YML105C)|FD-Score:-3.28|P-value:5.25E-4|Clearance:0||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:UTP15(YMR093W)|FD-Score:4.43|P-value:4.64E-6|Clearance:0.12||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP9(YHR196W)|FD-Score:3.41|P-value:3.26E-4|Clearance:0.03||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL152W(YDL152W_d)|FD-Score:3.94|P-value:4.02E-5|Clearance:0.23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YGR265W(YGR265W_d)|FD-Score:3.1|P-value:9.72E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YNL114C(YNL114C_d)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:ACT1(YFL039C)|FD-Score:-3.63|P-value:1.44E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ASA1(YPR085C)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.08||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CLP1(YOR250C)|FD-Score:4.57|P-value:2.49E-6|Clearance:0.13||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:GCD2(YGR083C)|FD-Score:4.86|P-value:5.73E-7|Clearance:0.3||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI18(YBR004C)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.06||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:KRE5(YOR336W)|FD-Score:4.31|P-value:8.21E-6|Clearance:0.07||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:NAM9(YNL137C)|FD-Score:3.1|P-value:9.52E-4|Clearance:0.01||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:NAT2(YGR147C)|FD-Score:3.55|P-value:1.93E-4|Clearance:0.01||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NOG2(YNR053C)|FD-Score:3.38|P-value:3.59E-4|Clearance:0.19||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:RPC53(YDL150W)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.13||SGD DESC:RNA polymerase III subunit C53 Gene:RRP4(YHR069C)|FD-Score:4.24|P-value:1.13E-5|Clearance:0.29||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC65(YML105C)|FD-Score:-3.28|P-value:5.25E-4|Clearance:0||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:UTP15(YMR093W)|FD-Score:4.43|P-value:4.64E-6|Clearance:0.12||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP9(YHR196W)|FD-Score:3.41|P-value:3.26E-4|Clearance:0.03||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL152W(YDL152W_d)|FD-Score:3.94|P-value:4.02E-5|Clearance:0.23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YGR265W(YGR265W_d)|FD-Score:3.1|P-value:9.72E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YNL114C(YNL114C_d)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3931349
Download HOP data (tab-delimited text)  (excel)
Gene:ADE8(YDR408C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ALF1(YNL148C)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ANP1(YEL036C)|FD-Score:3.93|P-value:4.25E-5||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:APM4(YOL062C)|FD-Score:-4.12|P-value:1.86E-5||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:BTN2(YGR142W)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:CAF4(YKR036C)|FD-Score:3.76|P-value:8.64E-5||SGD DESC:WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication Gene:CBP6(YBR120C)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:CDH1(YGL003C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:COG7(YGL005C)|FD-Score:4.38|P-value:5.84E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COR1(YBL045C)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain Gene:COX18(YGR062C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:CSF1(YLR087C)|FD-Score:-4.36|P-value:6.58E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CTI6(YPL181W)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:DSE2(YHR143W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DSF2(YBR007C_p)|FD-Score:4.02|P-value:2.85E-5||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FBP1(YLR377C)|FD-Score:3.98|P-value:3.44E-5||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FUI1(YBL042C)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GAL4(YPL248C)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p Gene:GAL7(YBR018C)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GEX2(YKR106W)|FD-Score:4.41|P-value:5.24E-6||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GLO3(YER122C)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:HIS1(YER055C)|FD-Score:7.54|P-value:2.44E-14||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HMT1(YBR034C)|FD-Score:7.05|P-value:8.99E-13||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HOR7(YMR251W-A)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HUG1(YML058W-A)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:IES5(YER092W)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:IRC15(YPL017C)|FD-Score:5.43|P-value:2.75E-8||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:ISU2(YOR226C)|FD-Score:-3.42|P-value:3.15E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:KRE2(YDR483W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:KTI12(YKL110C)|FD-Score:5.05|P-value:2.16E-7||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:MET32(YDR253C)|FD-Score:-4.36|P-value:6.39E-6||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MFT1(YML062C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MLS1(YNL117W)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MMM1(YLL006W)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRPL17(YNL252C)|FD-Score:4.47|P-value:3.96E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MYO4(YAL029C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:NPP2(YEL016C)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:NSG1(YHR133C)|FD-Score:-4.93|P-value:4.12E-7||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:ORM1(YGR038W)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PGD1(YGL025C)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PIH1(YHR034C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PKH3(YDR466W)|FD-Score:4.56|P-value:2.51E-6||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:RFU1(YLR073C)|FD-Score:4.27|P-value:9.75E-6||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RPL16B(YNL069C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RPL27A(YHR010W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:3.79|P-value:7.64E-5||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRG7(YOR305W)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:SCH9(YHR205W)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan Gene:SEM1(YDR363W-A)|FD-Score:4.53|P-value:2.94E-6||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SHM2(YLR058C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SSO1(YPL232W)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:SYO1(YDL063C)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TAT2(YOL020W)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TRP4(YDR354W)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP6(YFR010W)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VAC17(YCL063W)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:YDR149C(YDR149C_d)|FD-Score:4.55|P-value:2.68E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YMR057C(YMR057C_d)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YMR158C-A(YMR158C-A_p)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene Gene:YOR114W(YOR114W_p)|FD-Score:5.8|P-value:3.32E-9||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YPL247C(YPL247C_p)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YRB30(YGL164C)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes Gene:ADE8(YDR408C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ALF1(YNL148C)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ANP1(YEL036C)|FD-Score:3.93|P-value:4.25E-5||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:APM4(YOL062C)|FD-Score:-4.12|P-value:1.86E-5||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:BTN2(YGR142W)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:CAF4(YKR036C)|FD-Score:3.76|P-value:8.64E-5||SGD DESC:WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication Gene:CBP6(YBR120C)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:CDH1(YGL003C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:COG7(YGL005C)|FD-Score:4.38|P-value:5.84E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COR1(YBL045C)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain Gene:COX18(YGR062C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:CSF1(YLR087C)|FD-Score:-4.36|P-value:6.58E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CTI6(YPL181W)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:DSE2(YHR143W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DSF2(YBR007C_p)|FD-Score:4.02|P-value:2.85E-5||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FBP1(YLR377C)|FD-Score:3.98|P-value:3.44E-5||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FUI1(YBL042C)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GAL4(YPL248C)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p Gene:GAL7(YBR018C)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GEX2(YKR106W)|FD-Score:4.41|P-value:5.24E-6||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GLO3(YER122C)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:HIS1(YER055C)|FD-Score:7.54|P-value:2.44E-14||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HMT1(YBR034C)|FD-Score:7.05|P-value:8.99E-13||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HOR7(YMR251W-A)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HUG1(YML058W-A)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:IES5(YER092W)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:IRC15(YPL017C)|FD-Score:5.43|P-value:2.75E-8||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:ISU2(YOR226C)|FD-Score:-3.42|P-value:3.15E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:KRE2(YDR483W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:KTI12(YKL110C)|FD-Score:5.05|P-value:2.16E-7||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:MET32(YDR253C)|FD-Score:-4.36|P-value:6.39E-6||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MFT1(YML062C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MLS1(YNL117W)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MMM1(YLL006W)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRPL17(YNL252C)|FD-Score:4.47|P-value:3.96E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MYO4(YAL029C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:NPP2(YEL016C)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:NSG1(YHR133C)|FD-Score:-4.93|P-value:4.12E-7||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:ORM1(YGR038W)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PGD1(YGL025C)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PIH1(YHR034C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PKH3(YDR466W)|FD-Score:4.56|P-value:2.51E-6||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:RFU1(YLR073C)|FD-Score:4.27|P-value:9.75E-6||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RPL16B(YNL069C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RPL27A(YHR010W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:3.79|P-value:7.64E-5||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRG7(YOR305W)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:SCH9(YHR205W)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan Gene:SEM1(YDR363W-A)|FD-Score:4.53|P-value:2.94E-6||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SHM2(YLR058C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SSO1(YPL232W)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:SYO1(YDL063C)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TAT2(YOL020W)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TRP4(YDR354W)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP6(YFR010W)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VAC17(YCL063W)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:YDR149C(YDR149C_d)|FD-Score:4.55|P-value:2.68E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YMR057C(YMR057C_d)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YMR158C-A(YMR158C-A_p)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene Gene:YOR114W(YOR114W_p)|FD-Score:5.8|P-value:3.32E-9||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YPL247C(YPL247C_p)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YRB30(YGL164C)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR083C4.865.73E-70.30GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YOR250C4.572.49E-60.13CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YMR093W4.434.64E-60.12UTP15Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YOR336W4.318.21E-60.07KRE5Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects
YHR069C4.241.13E-50.29RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YDL152W_d3.944.02E-50.23YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YBR004C3.721.01E-40.06GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YNL114C_d3.661.27E-40.11YNL114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
YGR147C3.551.93E-40.01NAT2Protein with an apparent role in acetylation of N-terminal methionine residues
YDL150W3.542.00E-40.13RPC53RNA polymerase III subunit C53
YHR196W3.413.26E-40.03UTP9Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YNR053C3.383.59E-40.19NOG2Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation
YPR085C3.197.13E-40.08ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
YNL137C3.109.52E-40.01NAM9Mitochondrial ribosomal component of the small subunit
YGR265W_d3.109.72E-40.01YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER055C7.542.44E-14HIS1ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control
YBR034C7.058.99E-13HMT1Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region.
YOR114W_p5.803.32E-9YOR114W_pPutative protein of unknown function; null mutant is viable
YPL017C5.432.75E-8IRC15Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci
YKL110C5.052.16E-7KTI12Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
YDR466W4.562.51E-6PKH3Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
YDR149C_d4.552.68E-6YDR149C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth
YDR363W-A4.532.94E-6SEM1Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress
YNL252C4.473.96E-6MRPL17Mitochondrial ribosomal protein of the large subunit
YKR106W4.415.24E-6GEX2Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway
YGL005C4.385.84E-6COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YLR073C4.279.75E-6RFU1Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes
YHR034C4.261.04E-5PIH1Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II
YCL063W4.221.20E-5VAC17Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p
YBR007C_p4.022.85E-5DSF2_pDeletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress

GO enrichment analysis for SGTC_1768
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0936.97E-13SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.0740741
0.0939.63E-13SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.101266
0.0921.72E-12SGTC_392N-ethylmaleimide 8.3 μMMiscellaneous43620.121212RNA pol III & RNase P/MRP
0.0863.18E-11SGTC_15852',4'-dihydroxychalcone 10.4 μMTimTec (Pure natural product library)53572180.0740741
0.0807.90E-10SGTC_10214476-0760 85.3 μMChemDiv (Drug-like library)7467680.076087
0.0782.03E-9SGTC_2508celastrol 40.5 μMMicrosource (Natural product library)1227240.0769231PDR1
0.0764.48E-9SGTC_12730849-0096 14.1 μMChemDiv (Drug-like library)52860250.164557RPP1 & pyrimidine depletion
0.0757.63E-9SGTC_1471k673-0312 37.0 μMChemDiv (Drug-like library)240998840.0625RNA processing & uracil transport
0.0741.52E-8SGTC_1068loratadine 26.9 μMMiscellaneous39570.111111
0.0714.39E-8SGTC_190279-0214 38.4 μMChemDiv (Drug-like library)35662740.0506329TRP & mitochondrial translation
0.0714.63E-8SGTC_8600443-0016 25.1 μMChemDiv (Drug-like library)53379420.0722892
0.0691.21E-7SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.13684260S ribosome export
0.0672.25E-7SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.0740741
0.0664.11E-7SGTC_14740330-0125 5.3 μMChemDiv (Drug-like library)X14740.076087
0.0664.18E-7SGTC_20995377090 177.4 μMChembridge (Fragment library)22438290.125

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_9963970-07991.8 μM0.3623196615648ChemDiv (Drug-like library)299.321181.704
SGTC_2103551-044556.83 μM0.33752865180ChemDiv (Drug-like library)371.406983.0316
SGTC_2483572905027.16 μM0.33752865180Miscellaneous371.406983.0316
SGTC_1493970-082246.1 μM0.3205133114250ChemDiv (Drug-like library)313.347761.90504tubulin folding & SWR complex
SGTC_11323456-36142.5 μM0.31253125502ChemDiv (Drug-like library)318.350961.70116tubulin folding & SWR complex
SGTC_7353076-0345636 μM0.3026322888990ChemDiv (Drug-like library)314.421943.39513
SGTC_2465580924233.21 μM0.2839512731748Miscellaneous357.38042.68116
SGTC_2487591837344.05 μM0.2771082876279Miscellaneous385.39052.46517
SGTC_2474532157043.32 μM0.2739732841384Miscellaneous297.37153.55303
SGTC_5891227-007034.1 μM0.2739732841384ChemDiv (Drug-like library)297.37153.55303