1327-0068

2-chloro-4-(4-chlorophenoxy)-1-phenylpentane-1,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_177
Screen concentration 8.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 15389900
SMILES CC(C(=O)C(C(=O)C1=CC=CC=C1)Cl)OC2=CC=C(C=C2)Cl
Standardized SMILES CC(Oc1ccc(Cl)cc1)C(=O)C(Cl)C(=O)c2ccccc2
Molecular weight 337.1973
ALogP 4.68
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 25.05
% growth inhibition (Hom. pool) 19.22


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 15389900
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.05||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:CCT3(YJL014W)|FD-Score:-5.15|P-value:1.28E-7|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC20(YGL116W)|FD-Score:3.66|P-value:1.28E-4|Clearance:0.21||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:DBP5(YOR046C)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p Gene:DIP2(YLR129W)|FD-Score:-6.03|P-value:8.05E-10|Clearance:0||SGD DESC:Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex Gene:DML1(YMR211W)|FD-Score:6.22|P-value:2.53E-10|Clearance:0.16||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERB1(YMR049C)|FD-Score:4.94|P-value:3.89E-7|Clearance:0.08||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:FOL3(YMR113W)|FD-Score:4.24|P-value:1.09E-5|Clearance:0||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:GCD2(YGR083C)|FD-Score:7|P-value:1.32E-12|Clearance:0.78||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GLC7(YER133W)|FD-Score:-3.94|P-value:4.07E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM12(YDR047W)|FD-Score:3.84|P-value:6.24E-5|Clearance:0.01||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:ILV5(YLR355C)|FD-Score:4.34|P-value:7.24E-6|Clearance:0.09||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:MIA40(YKL195W)|FD-Score:11|P-value:2.18E-28|Clearance:2.25||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NAT2(YGR147C)|FD-Score:3.19|P-value:7.09E-4|Clearance:0.25||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:PKC1(YBL105C)|FD-Score:3.24|P-value:5.93E-4|Clearance:0.05||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RBA50(YDR527W)|FD-Score:5.98|P-value:1.10E-9|Clearance:0.61||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RER2(YBR002C)|FD-Score:4.58|P-value:2.35E-6|Clearance:0.06||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RET1(YOR207C)|FD-Score:24.4|P-value:9.22E-132|Clearance:8.14||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RHO1(YPR165W)|FD-Score:-3.48|P-value:2.52E-4|Clearance:0||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RIM2(YBR192W)|FD-Score:5.28|P-value:6.46E-8|Clearance:0.08||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPB7(YDR404C)|FD-Score:-4.29|P-value:8.95E-6|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPM2(YML091C)|FD-Score:4.52|P-value:3.11E-6|Clearance:0.18||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RPN2(YIL075C)|FD-Score:4.07|P-value:2.36E-5|Clearance:0.02||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RPO26(YPR187W)|FD-Score:3.35|P-value:4.04E-4|Clearance:0.11||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPT3(YDR394W)|FD-Score:3.79|P-value:7.58E-5|Clearance:0.09||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RSC58(YLR033W)|FD-Score:23.1|P-value:3.46E-118|Clearance:8.14||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:4.86|P-value:5.91E-7|Clearance:0.28||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:8.74|P-value:1.14E-18|Clearance:1.75||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:6.06|P-value:6.93E-10|Clearance:0.07||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC31(YDL195W)|FD-Score:3.83|P-value:6.54E-5|Clearance:0.04||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SPC105(YGL093W)|FD-Score:5.2|P-value:9.94E-8|Clearance:0.15||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SPC42(YKL042W)|FD-Score:-3.62|P-value:1.50E-4|Clearance:0||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SSL1(YLR005W)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.09||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:STH1(YIL126W)|FD-Score:5.05|P-value:2.23E-7|Clearance:0.11||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUI2(YJR007W)|FD-Score:5.38|P-value:3.79E-8|Clearance:0.1||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TFA1(YKL028W)|FD-Score:-3.52|P-value:2.13E-4|Clearance:0||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TIF35(YDR429C)|FD-Score:-6.93|P-value:2.10E-12|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOM40(YMR203W)|FD-Score:14.9|P-value:8.67E-51|Clearance:3.95||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TSC10(YBR265W)|FD-Score:4.24|P-value:1.11E-5|Clearance:0.17||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:YGR115C(YGR115C_d)|FD-Score:3.45|P-value:2.84E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YPT1(YFL038C)|FD-Score:4.05|P-value:2.57E-5|Clearance:0.21||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) Gene:ACC1(YNR016C)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.05||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:CCT3(YJL014W)|FD-Score:-5.15|P-value:1.28E-7|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC20(YGL116W)|FD-Score:3.66|P-value:1.28E-4|Clearance:0.21||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:DBP5(YOR046C)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p Gene:DIP2(YLR129W)|FD-Score:-6.03|P-value:8.05E-10|Clearance:0||SGD DESC:Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex Gene:DML1(YMR211W)|FD-Score:6.22|P-value:2.53E-10|Clearance:0.16||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERB1(YMR049C)|FD-Score:4.94|P-value:3.89E-7|Clearance:0.08||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:FOL3(YMR113W)|FD-Score:4.24|P-value:1.09E-5|Clearance:0||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:GCD2(YGR083C)|FD-Score:7|P-value:1.32E-12|Clearance:0.78||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GLC7(YER133W)|FD-Score:-3.94|P-value:4.07E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM12(YDR047W)|FD-Score:3.84|P-value:6.24E-5|Clearance:0.01||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:ILV5(YLR355C)|FD-Score:4.34|P-value:7.24E-6|Clearance:0.09||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:MIA40(YKL195W)|FD-Score:11|P-value:2.18E-28|Clearance:2.25||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NAT2(YGR147C)|FD-Score:3.19|P-value:7.09E-4|Clearance:0.25||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:PKC1(YBL105C)|FD-Score:3.24|P-value:5.93E-4|Clearance:0.05||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RBA50(YDR527W)|FD-Score:5.98|P-value:1.10E-9|Clearance:0.61||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RER2(YBR002C)|FD-Score:4.58|P-value:2.35E-6|Clearance:0.06||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RET1(YOR207C)|FD-Score:24.4|P-value:9.22E-132|Clearance:8.14||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RHO1(YPR165W)|FD-Score:-3.48|P-value:2.52E-4|Clearance:0||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RIM2(YBR192W)|FD-Score:5.28|P-value:6.46E-8|Clearance:0.08||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPB7(YDR404C)|FD-Score:-4.29|P-value:8.95E-6|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPM2(YML091C)|FD-Score:4.52|P-value:3.11E-6|Clearance:0.18||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RPN2(YIL075C)|FD-Score:4.07|P-value:2.36E-5|Clearance:0.02||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RPO26(YPR187W)|FD-Score:3.35|P-value:4.04E-4|Clearance:0.11||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPT3(YDR394W)|FD-Score:3.79|P-value:7.58E-5|Clearance:0.09||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RSC58(YLR033W)|FD-Score:23.1|P-value:3.46E-118|Clearance:8.14||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:4.86|P-value:5.91E-7|Clearance:0.28||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:8.74|P-value:1.14E-18|Clearance:1.75||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:6.06|P-value:6.93E-10|Clearance:0.07||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC31(YDL195W)|FD-Score:3.83|P-value:6.54E-5|Clearance:0.04||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SPC105(YGL093W)|FD-Score:5.2|P-value:9.94E-8|Clearance:0.15||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SPC42(YKL042W)|FD-Score:-3.62|P-value:1.50E-4|Clearance:0||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SSL1(YLR005W)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.09||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:STH1(YIL126W)|FD-Score:5.05|P-value:2.23E-7|Clearance:0.11||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUI2(YJR007W)|FD-Score:5.38|P-value:3.79E-8|Clearance:0.1||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TFA1(YKL028W)|FD-Score:-3.52|P-value:2.13E-4|Clearance:0||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TIF35(YDR429C)|FD-Score:-6.93|P-value:2.10E-12|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOM40(YMR203W)|FD-Score:14.9|P-value:8.67E-51|Clearance:3.95||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TSC10(YBR265W)|FD-Score:4.24|P-value:1.11E-5|Clearance:0.17||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:YGR115C(YGR115C_d)|FD-Score:3.45|P-value:2.84E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YPT1(YFL038C)|FD-Score:4.05|P-value:2.57E-5|Clearance:0.21||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 15389900
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:5.16|P-value:1.26E-7||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ALD6(YPL061W)|FD-Score:7.45|P-value:4.51E-14||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ALT1(YLR089C)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive Gene:ARA1(YBR149W)|FD-Score:-4.15|P-value:1.63E-5||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ARC1(YGL105W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ATS1(YAL020C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BOR1(YNL275W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:BUD19(YJL188C_d)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAF130(YGR134W)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation Gene:CCS1(YMR038C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CIN2(YPL241C)|FD-Score:3.75|P-value:8.84E-5||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CKA1(YIL035C)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLA4(YNL298W)|FD-Score:8.88|P-value:3.40E-19||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLB5(YPR120C)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:CNA1(YLR433C)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:CTR1(YPR124W)|FD-Score:8.78|P-value:8.00E-19||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYB5(YNL111C)|FD-Score:6.36|P-value:1.01E-10||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DOA4(YDR069C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:ECM11(YDR446W)|FD-Score:-3.86|P-value:5.75E-5||SGD DESC:Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure Gene:EGD2(YHR193C)|FD-Score:10.4|P-value:9.26E-26||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:ELA1(YNL230C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:FCJ1(YKR016W)|FD-Score:-4.23|P-value:1.19E-5||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:GEP5(YLR091W)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GET1(YGL020C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GET2(YER083C)|FD-Score:9.1|P-value:4.49E-20||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:HAP4(YKL109W)|FD-Score:-5.64|P-value:8.33E-9||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HCR1(YLR192C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HOL1(YNR055C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake Gene:HOM6(YJR139C)|FD-Score:6.94|P-value:1.95E-12||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:ICE2(YIL090W)|FD-Score:5.02|P-value:2.61E-7||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IES6(YEL044W)|FD-Score:4|P-value:3.19E-5||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:ISA1(YLL027W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:LST7(YGR057C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LTE1(YAL024C)|FD-Score:4.38|P-value:5.82E-6||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAC1(YMR021C)|FD-Score:11.5|P-value:1.11E-30||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAL31(YBR298C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MHR1(YDR296W)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MIR1(YJR077C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MMS22(YLR320W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MRH4(YGL064C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRP7(YNL005C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL15(YLR312W-A)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL38(YKL170W)|FD-Score:4.41|P-value:5.23E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPS8(YMR158W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTG1(YMR097C)|FD-Score:4.84|P-value:6.61E-7||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NAT1(YDL040C)|FD-Score:3.75|P-value:8.94E-5||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NEW1(YPL226W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NPR1(YNL183C)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:PDX1(YGR193C)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core Gene:PIF1(YML061C)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PIH1(YHR034C)|FD-Score:6.6|P-value:1.99E-11||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:POL32(YJR043C)|FD-Score:9.42|P-value:2.31E-21||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PRO2(YOR323C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RAD27(YKL113C)|FD-Score:5.01|P-value:2.70E-7||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:REV7(YIL139C)|FD-Score:-3.96|P-value:3.70E-5||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RML2(YEL050C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:ROM2(YLR371W)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPE1(YJL121C)|FD-Score:9.69|P-value:1.60E-22||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL31A(YDL075W)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:4.77|P-value:9.09E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RSM25(YIL093C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SET4(YJL105W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SGS1(YMR190C)|FD-Score:3.76|P-value:8.35E-5||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SHB17(YKR043C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SKN7(YHR206W)|FD-Score:7.89|P-value:1.51E-15||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SOD1(YJR104C)|FD-Score:7.34|P-value:1.03E-13||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPS100(YHR139C)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SRO77(YBL106C)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SRO9(YCL037C)|FD-Score:4.94|P-value:3.89E-7||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SRS2(YJL092W)|FD-Score:4.89|P-value:5.11E-7||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SUV3(YPL029W)|FD-Score:4.9|P-value:4.89E-7||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TDA9(YML081W)|FD-Score:6.02|P-value:8.59E-10||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TFB5(YDR079C-A)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:THR1(YHR025W)|FD-Score:19.3|P-value:3.49E-83||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:THR4(YCR053W)|FD-Score:8.81|P-value:6.49E-19||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TKL1(YPR074C)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TMA64(YDR117C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:TPO3(YPR156C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:11.3|P-value:8.35E-30||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:7.46|P-value:4.30E-14||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:TUF1(YOR187W)|FD-Score:3.87|P-value:5.39E-5||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UTR1(YJR049C)|FD-Score:36.4|P-value:7.67E-290||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VAC17(YCL063W)|FD-Score:4.58|P-value:2.30E-6||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:YAP1(YML007W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL172C(YDL172C_d)|FD-Score:-3.1|P-value:9.75E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR537C(YDR537C_d)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YGR127W(YGR127W_p)|FD-Score:-3.22|P-value:6.38E-4||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YHB1(YGR234W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YHR050W-A(YHR050W-A_p)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL120W(YJL120W_d)|FD-Score:7.16|P-value:3.96E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJR018W(YJR018W_d)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL123W(YKL123W_d)|FD-Score:-4.07|P-value:2.34E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4 Gene:YLR118C(YLR118C)|FD-Score:-4.18|P-value:1.48E-5||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR312C(YLR312C_p)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Putative protein of unknown function Gene:YNR021W(YNR021W_p)|FD-Score:-3.93|P-value:4.19E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene Gene:YNR048W(YNR048W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOL075C(YOL075C_p)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Putative ABC transporter Gene:YOR309C(YOR309C_d)|FD-Score:9.91|P-value:1.86E-23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YOS9(YDR057W)|FD-Score:-3.16|P-value:7.99E-4||SGD DESC:ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family Gene:YPK1(YKL126W)|FD-Score:5.29|P-value:6.12E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL062W(YPL062W_d)|FD-Score:8.22|P-value:1.02E-16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPR099C(YPR099C_d)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:AAT2(YLR027C)|FD-Score:5.16|P-value:1.26E-7||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ALD6(YPL061W)|FD-Score:7.45|P-value:4.51E-14||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ALT1(YLR089C)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive Gene:ARA1(YBR149W)|FD-Score:-4.15|P-value:1.63E-5||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ARC1(YGL105W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ATS1(YAL020C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BOR1(YNL275W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:BUD19(YJL188C_d)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAF130(YGR134W)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation Gene:CCS1(YMR038C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CIN2(YPL241C)|FD-Score:3.75|P-value:8.84E-5||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CKA1(YIL035C)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLA4(YNL298W)|FD-Score:8.88|P-value:3.40E-19||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLB5(YPR120C)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:CNA1(YLR433C)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:CTR1(YPR124W)|FD-Score:8.78|P-value:8.00E-19||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYB5(YNL111C)|FD-Score:6.36|P-value:1.01E-10||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DOA4(YDR069C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:ECM11(YDR446W)|FD-Score:-3.86|P-value:5.75E-5||SGD DESC:Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure Gene:EGD2(YHR193C)|FD-Score:10.4|P-value:9.26E-26||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:ELA1(YNL230C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:FCJ1(YKR016W)|FD-Score:-4.23|P-value:1.19E-5||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:GEP5(YLR091W)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GET1(YGL020C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GET2(YER083C)|FD-Score:9.1|P-value:4.49E-20||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:HAP4(YKL109W)|FD-Score:-5.64|P-value:8.33E-9||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HCR1(YLR192C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HOL1(YNR055C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake Gene:HOM6(YJR139C)|FD-Score:6.94|P-value:1.95E-12||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:ICE2(YIL090W)|FD-Score:5.02|P-value:2.61E-7||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IES6(YEL044W)|FD-Score:4|P-value:3.19E-5||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:ISA1(YLL027W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:LST7(YGR057C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LTE1(YAL024C)|FD-Score:4.38|P-value:5.82E-6||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAC1(YMR021C)|FD-Score:11.5|P-value:1.11E-30||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAL31(YBR298C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MHR1(YDR296W)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MIR1(YJR077C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MMS22(YLR320W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MRH4(YGL064C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRP7(YNL005C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL15(YLR312W-A)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL38(YKL170W)|FD-Score:4.41|P-value:5.23E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPS8(YMR158W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTG1(YMR097C)|FD-Score:4.84|P-value:6.61E-7||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NAT1(YDL040C)|FD-Score:3.75|P-value:8.94E-5||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NEW1(YPL226W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NPR1(YNL183C)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:PDX1(YGR193C)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core Gene:PIF1(YML061C)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PIH1(YHR034C)|FD-Score:6.6|P-value:1.99E-11||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:POL32(YJR043C)|FD-Score:9.42|P-value:2.31E-21||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PRO2(YOR323C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RAD27(YKL113C)|FD-Score:5.01|P-value:2.70E-7||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:REV7(YIL139C)|FD-Score:-3.96|P-value:3.70E-5||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RML2(YEL050C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:ROM2(YLR371W)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPE1(YJL121C)|FD-Score:9.69|P-value:1.60E-22||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL31A(YDL075W)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:4.77|P-value:9.09E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RSM25(YIL093C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SET4(YJL105W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SGS1(YMR190C)|FD-Score:3.76|P-value:8.35E-5||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SHB17(YKR043C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SKN7(YHR206W)|FD-Score:7.89|P-value:1.51E-15||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SOD1(YJR104C)|FD-Score:7.34|P-value:1.03E-13||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPS100(YHR139C)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SRO77(YBL106C)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SRO9(YCL037C)|FD-Score:4.94|P-value:3.89E-7||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SRS2(YJL092W)|FD-Score:4.89|P-value:5.11E-7||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SUV3(YPL029W)|FD-Score:4.9|P-value:4.89E-7||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TDA9(YML081W)|FD-Score:6.02|P-value:8.59E-10||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TFB5(YDR079C-A)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:THR1(YHR025W)|FD-Score:19.3|P-value:3.49E-83||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:THR4(YCR053W)|FD-Score:8.81|P-value:6.49E-19||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TKL1(YPR074C)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TMA64(YDR117C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:TPO3(YPR156C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:11.3|P-value:8.35E-30||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:7.46|P-value:4.30E-14||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:TUF1(YOR187W)|FD-Score:3.87|P-value:5.39E-5||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UTR1(YJR049C)|FD-Score:36.4|P-value:7.67E-290||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VAC17(YCL063W)|FD-Score:4.58|P-value:2.30E-6||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:YAP1(YML007W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL172C(YDL172C_d)|FD-Score:-3.1|P-value:9.75E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR537C(YDR537C_d)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YGR127W(YGR127W_p)|FD-Score:-3.22|P-value:6.38E-4||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YHB1(YGR234W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YHR050W-A(YHR050W-A_p)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL120W(YJL120W_d)|FD-Score:7.16|P-value:3.96E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJR018W(YJR018W_d)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL123W(YKL123W_d)|FD-Score:-4.07|P-value:2.34E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4 Gene:YLR118C(YLR118C)|FD-Score:-4.18|P-value:1.48E-5||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR312C(YLR312C_p)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Putative protein of unknown function Gene:YNR021W(YNR021W_p)|FD-Score:-3.93|P-value:4.19E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene Gene:YNR048W(YNR048W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOL075C(YOL075C_p)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Putative ABC transporter Gene:YOR309C(YOR309C_d)|FD-Score:9.91|P-value:1.86E-23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YOS9(YDR057W)|FD-Score:-3.16|P-value:7.99E-4||SGD DESC:ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family Gene:YPK1(YKL126W)|FD-Score:5.29|P-value:6.12E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL062W(YPL062W_d)|FD-Score:8.22|P-value:1.02E-16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPR099C(YPR099C_d)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR207C24.409.22E-1328.14RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YLR033W23.103.46E-1188.14RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YMR203W14.908.67E-513.95TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YKL195W11.002.18E-282.25MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YFR037C8.741.14E-181.75RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YGR083C7.001.32E-120.78GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YMR211W6.222.53E-100.16DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YML127W6.066.93E-100.07RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YDR527W5.981.10E-90.61RBA50Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
YJR007W5.383.79E-80.10SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YBR192W5.286.46E-80.08RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YGL093W5.209.94E-80.15SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YIL126W5.052.23E-70.11STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YMR049C4.943.89E-70.08ERB1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1
YCR052W4.865.91E-70.28RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJR049C36.407.67E-290UTR1ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
YHR025W19.303.49E-83THR1Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
YMR021C11.501.11E-30MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YBR058C-A11.308.35E-30TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YHR193C10.409.26E-26EGD2Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes
YOR309C_d9.911.86E-23YOR309C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
YJL121C9.691.60E-22RPE1D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress
YJR043C9.422.31E-21POL32Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p
YER083C9.104.49E-20GET2Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division
YNL298W8.883.40E-19CLA4Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
YCR053W8.816.49E-19THR4Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
YPR124W8.788.00E-19CTR1High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress
YPL062W_d8.221.02E-16YPL062W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation
YHR206W7.891.51E-15SKN7Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication
YML124C7.464.30E-14TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_177
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4690SGTC_2233sulbentine 40.4 μMMiscellaneous676860.101695
0.4505.91E-293SGTC_2510benzyl isothiocyanate 8.3 μMMicrosource (Natural product library)23460.122449
0.3244.89E-144SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.117647
0.3072.33E-129SGTC_24305571206 200.0 μMMiscellaneous17317710.1
0.3005.02E-123SGTC_22236903751 107.6 μMChembridge (Fragment library)55988630.0857143superoxide
0.2849.67E-110SGTC_2408sulbentine 18.4 μMMiscellaneous676860.101695
0.2811.24E-106SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.166667
0.2612.20E-92SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.0945946
0.2554.08E-88SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.0933333
0.2512.95E-85SGTC_23447990960 77.0 μMChembridge (Fragment library)1408130.163265
0.2463.52E-82SGTC_6113453-1652 23.7 μMChemDiv (Drug-like library)15605750.128571
0.2462.88E-81SGTC_460329-0187a 2.2 μMChemDiv (Drug-like library)464948170.214286
0.2452.93E-81SGTC_28pentachloropyridine N-oxide 1.6 μMChemDiv (Drug-like library)1402500.0666667TSC3-RPN4
0.2411.15E-78SGTC_27997812892 81.8 μMChembridge (Drug-like library)202052350.137931superoxide
0.2354.67E-75SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.163636TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1608st00147340.5 μM0.3478267292267TimTec (Natural product derivative library)242.700244.36601
SGTC_11911487-116610.6 μM0.3461543955771ChemDiv (Drug-like library)293.748743.24304azole & statin
SGTC_70192-15824.23 μM0.34252616ChemDiv (Drug-like library)398.089164.78702
SGTC_2777climbazole1.54 μM0.32142937907Miscellaneous292.760683.76603iron homeostasis
SGTC_2841900476138.96 μM0.3214292991039Chembridge (Drug-like library)289.756744.08412amide catabolism
SGTC_6530203-001655.2 μM0.320755295302ChemDiv (Drug-like library)289.756743.44212
SGTC_1300136-0219337.66 μM0.307692234244ChemDiv (Drug-like library)296.148643.77812
SGTC_3056909490049.47 μM0.30508525236784Chembridge (Drug-like library)342.4072233.88404
SGTC_2882905804458.44 μM0.30357118076804Chembridge (Drug-like library)273.3021433.62513
SGTC_8700573-221357.6 μM0.2968753916321ChemDiv (Drug-like library)453.91636.78104ERG2