st048882

2-methoxy-5-(3-nitro-2H-chromen-2-yl)phenol

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1771
Screen concentration 4.2 μM
Source TimTec (Natural product derivative library)
PubChem CID 4580403
SMILES COC1=C(C=C(C=C1)C2C(=CC3=CC=CC=C3O2)[N+](=O)[O-])O
Standardized SMILES COc1ccc(cc1O)C2Oc3ccccc3C=C2[N+](=O)[O-]
Molecular weight 299.2781
ALogP 3.13
H-bond donor count 1
H-bond acceptor count 5
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 2.94
% growth inhibition (Hom. pool) -0.01


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4580403
Download HIP data (tab-delimited text)  (excel)
Gene:AME1(YBR211C)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC19(YAL038W)|FD-Score:-3.14|P-value:8.50E-4|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:GAB1(YLR459W)|FD-Score:-3.27|P-value:5.39E-4|Clearance:0||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:GPI8(YDR331W)|FD-Score:-4.22|P-value:1.23E-5|Clearance:0||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:HRP1(YOL123W)|FD-Score:-4.18|P-value:1.48E-5|Clearance:0||SGD DESC:Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences Gene:INN1(YNL152W)|FD-Score:4.3|P-value:8.60E-6|Clearance:0.01||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:NDC1(YML031W)|FD-Score:3.4|P-value:3.42E-4|Clearance:0.06||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NOP9(YJL010C)|FD-Score:6.15|P-value:3.78E-10|Clearance:1.47||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:PRP3(YDR473C)|FD-Score:4.29|P-value:9.00E-6|Clearance:0.16||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RDS3(YPR094W)|FD-Score:5.77|P-value:4.00E-9|Clearance:1.47||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:RFC5(YBR087W)|FD-Score:-3.49|P-value:2.42E-4|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPA190(YOR341W)|FD-Score:3.32|P-value:4.56E-4|Clearance:0.09||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPC53(YDL150W)|FD-Score:4.13|P-value:1.85E-5|Clearance:0.26||SGD DESC:RNA polymerase III subunit C53 Gene:RPN6(YDL097C)|FD-Score:3.1|P-value:9.80E-4|Clearance:0.07||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:7.04|P-value:9.49E-13|Clearance:1.47||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS3(YNL178W)|FD-Score:3.87|P-value:5.55E-5|Clearance:0.04||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RSC6(YCR052W)|FD-Score:6.33|P-value:1.24E-10|Clearance:1.47||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SPC42(YKL042W)|FD-Score:3.34|P-value:4.24E-4|Clearance:0.02||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:TAF7(YMR227C)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.03||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:TFC6(YDR362C)|FD-Score:3.8|P-value:7.31E-5|Clearance:0.38||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TOM22(YNL131W)|FD-Score:3.12|P-value:9.05E-4|Clearance:0.02||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TOM40(YMR203W)|FD-Score:3.83|P-value:6.46E-5|Clearance:0.03||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TUB1(YML085C)|FD-Score:5.99|P-value:1.04E-9|Clearance:1.47||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YJL086C(YJL086C_d)|FD-Score:3.15|P-value:8.12E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR339C(YLR339C_d)|FD-Score:-3.48|P-value:2.50E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Gene:YOR102W(YOR102W_d)|FD-Score:3.23|P-value:6.25E-4|Clearance:0.08||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Gene:AME1(YBR211C)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC19(YAL038W)|FD-Score:-3.14|P-value:8.50E-4|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:GAB1(YLR459W)|FD-Score:-3.27|P-value:5.39E-4|Clearance:0||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:GPI8(YDR331W)|FD-Score:-4.22|P-value:1.23E-5|Clearance:0||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:HRP1(YOL123W)|FD-Score:-4.18|P-value:1.48E-5|Clearance:0||SGD DESC:Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences Gene:INN1(YNL152W)|FD-Score:4.3|P-value:8.60E-6|Clearance:0.01||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:NDC1(YML031W)|FD-Score:3.4|P-value:3.42E-4|Clearance:0.06||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NOP9(YJL010C)|FD-Score:6.15|P-value:3.78E-10|Clearance:1.47||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:PRP3(YDR473C)|FD-Score:4.29|P-value:9.00E-6|Clearance:0.16||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RDS3(YPR094W)|FD-Score:5.77|P-value:4.00E-9|Clearance:1.47||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:RFC5(YBR087W)|FD-Score:-3.49|P-value:2.42E-4|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPA190(YOR341W)|FD-Score:3.32|P-value:4.56E-4|Clearance:0.09||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPC53(YDL150W)|FD-Score:4.13|P-value:1.85E-5|Clearance:0.26||SGD DESC:RNA polymerase III subunit C53 Gene:RPN6(YDL097C)|FD-Score:3.1|P-value:9.80E-4|Clearance:0.07||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:7.04|P-value:9.49E-13|Clearance:1.47||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS3(YNL178W)|FD-Score:3.87|P-value:5.55E-5|Clearance:0.04||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RSC6(YCR052W)|FD-Score:6.33|P-value:1.24E-10|Clearance:1.47||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SPC42(YKL042W)|FD-Score:3.34|P-value:4.24E-4|Clearance:0.02||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:TAF7(YMR227C)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.03||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:TFC6(YDR362C)|FD-Score:3.8|P-value:7.31E-5|Clearance:0.38||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TOM22(YNL131W)|FD-Score:3.12|P-value:9.05E-4|Clearance:0.02||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TOM40(YMR203W)|FD-Score:3.83|P-value:6.46E-5|Clearance:0.03||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TUB1(YML085C)|FD-Score:5.99|P-value:1.04E-9|Clearance:1.47||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YJL086C(YJL086C_d)|FD-Score:3.15|P-value:8.12E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR339C(YLR339C_d)|FD-Score:-3.48|P-value:2.50E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Gene:YOR102W(YOR102W_d)|FD-Score:3.23|P-value:6.25E-4|Clearance:0.08||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4580403
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALD2(YMR170C)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ALF1(YNL148C)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ALG5(YPL227C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ALT2(YDR111C_p)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p Gene:BIO2(YGR286C)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BIO4(YNR057C)|FD-Score:3.3|P-value:4.92E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:CCS1(YMR038C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC73(YLR418C)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLB2(YPR119W)|FD-Score:4.87|P-value:5.65E-7||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CLB5(YPR120C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:COX7(YMR256C)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CTR1(YPR124W)|FD-Score:4.95|P-value:3.74E-7||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CTR9(YOL145C)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DIF1(YLR437C)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:EST1(YLR233C)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA Gene:FAR11(YNL127W)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:GLK1(YCL040W)|FD-Score:-4.51|P-value:3.19E-6||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:GYP7(YDL234C)|FD-Score:-3.95|P-value:3.98E-5||SGD DESC:GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking Gene:GZF3(YJL110C)|FD-Score:-3.35|P-value:4.07E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HAT1(YPL001W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair Gene:HCR1(YLR192C)|FD-Score:3.83|P-value:6.44E-5||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HOM6(YJR139C)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HSC82(YMR186W)|FD-Score:4.28|P-value:9.54E-6||SGD DESC:Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication Gene:MAK10(YEL053C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MAL33(YBR297W)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MEP2(YNL142W)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MRPL23(YOR150W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH2(YOL090W)|FD-Score:3.79|P-value:7.52E-5||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:NUP120(YKL057C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:NUR1(YDL089W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OSW1(YOR255W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PAU7(YAR020C)|FD-Score:4.41|P-value:5.14E-6||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PEP7(YDR323C)|FD-Score:7.05|P-value:8.84E-13||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEP8(YJL053W)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PET123(YOR158W)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PRM6(YML047C)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family Gene:PTH1(YHR189W)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RCN1(YKL159C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:RHO2(YNL090W)|FD-Score:5.3|P-value:5.89E-8||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RPL20A(YMR242C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL29(YFR032C-A)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPN4(YDL020C)|FD-Score:6.11|P-value:4.95E-10||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT1(YBL048W_d)|FD-Score:5.18|P-value:1.10E-7||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RSB1(YOR049C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane Gene:RSC1(YGR056W)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SBE22(YHR103W)|FD-Score:3.83|P-value:6.35E-5||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SHE4(YOR035C)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SOL4(YGR248W)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SPO22(YIL073C)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:STP22(YCL008C)|FD-Score:6.04|P-value:7.74E-10||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:TAT1(YBR069C)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRP4(YDR354W)|FD-Score:3.93|P-value:4.17E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VPS36(YLR417W)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS62(YGR141W)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:XBP1(YIL101C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YAR030C(YAR030C_d)|FD-Score:-3.8|P-value:7.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 Gene:YER091C-A(YER091C-A_d)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFT2(YDR319C_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGR066C(YGR066C_p)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Putative protein of unknown function Gene:YGR266W(YGR266W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YGR269W(YGR269W_d)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YIL012W(YIL012W_d)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIR020W-A(YIR020W-A_d)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YMR245W(YMR245W_d)|FD-Score:6.18|P-value:3.29E-10||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL143C(YNL143C_p)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR214C(YOR214C_p)|FD-Score:-3.88|P-value:5.13E-5||SGD DESC:Putative protein of unknown function; YOR214C is not an essential gene Gene:YPR127W(YPR127W)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:AFT1(YGL071W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALD2(YMR170C)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ALF1(YNL148C)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ALG5(YPL227C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ALT2(YDR111C_p)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p Gene:BIO2(YGR286C)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BIO4(YNR057C)|FD-Score:3.3|P-value:4.92E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:CCS1(YMR038C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC73(YLR418C)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLB2(YPR119W)|FD-Score:4.87|P-value:5.65E-7||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CLB5(YPR120C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:COX7(YMR256C)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CTR1(YPR124W)|FD-Score:4.95|P-value:3.74E-7||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CTR9(YOL145C)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DIF1(YLR437C)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:EST1(YLR233C)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA Gene:FAR11(YNL127W)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:GLK1(YCL040W)|FD-Score:-4.51|P-value:3.19E-6||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:GYP7(YDL234C)|FD-Score:-3.95|P-value:3.98E-5||SGD DESC:GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking Gene:GZF3(YJL110C)|FD-Score:-3.35|P-value:4.07E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HAT1(YPL001W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair Gene:HCR1(YLR192C)|FD-Score:3.83|P-value:6.44E-5||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HOM6(YJR139C)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HSC82(YMR186W)|FD-Score:4.28|P-value:9.54E-6||SGD DESC:Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication Gene:MAK10(YEL053C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MAL33(YBR297W)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MEP2(YNL142W)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MRPL23(YOR150W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH2(YOL090W)|FD-Score:3.79|P-value:7.52E-5||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:NUP120(YKL057C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:NUR1(YDL089W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OSW1(YOR255W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PAU7(YAR020C)|FD-Score:4.41|P-value:5.14E-6||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PEP7(YDR323C)|FD-Score:7.05|P-value:8.84E-13||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEP8(YJL053W)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PET123(YOR158W)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PRM6(YML047C)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family Gene:PTH1(YHR189W)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RCN1(YKL159C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:RHO2(YNL090W)|FD-Score:5.3|P-value:5.89E-8||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RPL20A(YMR242C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL29(YFR032C-A)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPN4(YDL020C)|FD-Score:6.11|P-value:4.95E-10||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT1(YBL048W_d)|FD-Score:5.18|P-value:1.10E-7||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RSB1(YOR049C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane Gene:RSC1(YGR056W)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SBE22(YHR103W)|FD-Score:3.83|P-value:6.35E-5||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SHE4(YOR035C)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SOL4(YGR248W)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SPO22(YIL073C)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:STP22(YCL008C)|FD-Score:6.04|P-value:7.74E-10||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:TAT1(YBR069C)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRP4(YDR354W)|FD-Score:3.93|P-value:4.17E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VPS36(YLR417W)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS62(YGR141W)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:XBP1(YIL101C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YAR030C(YAR030C_d)|FD-Score:-3.8|P-value:7.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 Gene:YER091C-A(YER091C-A_d)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFT2(YDR319C_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGR066C(YGR066C_p)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Putative protein of unknown function Gene:YGR266W(YGR266W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YGR269W(YGR269W_d)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YIL012W(YIL012W_d)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIR020W-A(YIR020W-A_d)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YMR245W(YMR245W_d)|FD-Score:6.18|P-value:3.29E-10||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL143C(YNL143C_p)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR214C(YOR214C_p)|FD-Score:-3.88|P-value:5.13E-5||SGD DESC:Putative protein of unknown function; YOR214C is not an essential gene Gene:YPR127W(YPR127W)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C7.049.49E-131.47RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YCR052W6.331.24E-101.47RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YJL010C6.153.78E-101.47NOP9Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
YML085C5.991.04E-91.47TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YPR094W5.774.00E-91.47RDS3Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance
YNL152W4.308.60E-60.01INN1Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis
YDR473C4.299.00E-60.16PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YDL150W4.131.85E-50.26RPC53RNA polymerase III subunit C53
YNL178W3.875.55E-50.04RPS3Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3
YMR203W3.836.46E-50.03TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YDR362C3.807.31E-50.38TFC6One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
YMR227C3.423.11E-40.03TAF7TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation
YML031W3.403.42E-40.06NDC1Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
YKL042W3.344.24E-40.02SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YOR341W3.324.56E-40.09RPA190RNA polymerase I largest subunit A190

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR323C7.058.84E-13PEP7Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
YMR245W_d6.183.29E-10YMR245W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDL020C6.114.95E-10RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YCL008C6.047.74E-10STP22Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype
YNL090W5.305.89E-8RHO2Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly
YBL048W_d5.181.10E-7RRT1_dIdentified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
YPR124W4.953.74E-7CTR1High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress
YPR119W4.875.65E-7CLB2B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome
YBR069C4.621.93E-6TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YFR032C-A4.424.90E-6RPL29Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog
YAR020C4.415.14E-6PAU7Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme
YMR186W4.289.54E-6HSC82Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication
YOR049C4.211.26E-5RSB1Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane
YMR256C4.191.42E-5COX7Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
YOR158W4.121.86E-5PET123Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator

GO enrichment analysis for SGTC_1771
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1511.50E-31SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.138462
0.1484.06E-30SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.138462
0.1419.71E-28SGTC_14740330-0125 5.3 μMChemDiv (Drug-like library)X14740.075
0.1402.46E-27SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0833333TSC3-RPN4
0.1399.71E-27SGTC_2350330-0087 5.0 mMChemDiv (Drug-like library)464948180.118421TSC3-RPN4
0.1369.27E-26SGTC_575k072-0246 11.9 μMChemDiv (Drug-like library)67967790.108434
0.1321.84E-24SGTC_14780453-0656 35.7 μMChemDiv (Drug-like library)52584960.123288
0.1323.33E-24SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.0963855
0.1254.81E-22SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.08
0.1231.79E-21SGTC_1661st013063 9.7 μMTimTec (Natural product derivative library)7213950.12
0.1232.57E-21SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.126761
0.1223.91E-21SGTC_21135491891 107.0 μMChembridge (Fragment library)7899790.0882353TSC3-RPN4
0.1211.12E-20SGTC_9262268-1401 59.4 μMChemDiv (Drug-like library)29315780.157895TSC3-RPN4
0.1202.29E-20SGTC_15852',4'-dihydroxychalcone 10.4 μMTimTec (Pure natural product library)53572180.138462
0.1203.00E-20SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.0857143TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8890919-152482.7 μM0.3230773905452ChemDiv (Drug-like library)274.271962.85825
SGTC_2568diosmetin100 μM0.3076925281612Microsource (Natural product library)300.262882.39436
SGTC_1114scopoletin1.73 μM0.2931035280460TimTec (Natural product library)192.168121.64114
SGTC_6781218-217985.9 μM0.2878796820081ChemDiv (Drug-like library)272.256081.82515RNA pol III & RNase P/MRP
SGTC_15465-methoxyflavanone78.7 μM0.285714147795TimTec (Pure natural product library)254.280563.08303
SGTC_1863st05764120.1 μM0.2857143921635TimTec (Natural product derivative library)270.279962.61414
SGTC_1834st05598152.8 μM0.276923471719TimTec (Natural product derivative library)284.263482.63625RPP1 & pyrimidine depletion
SGTC_1995st07281461.1 μM0.2739735769371TimTec (Natural product derivative library)327.248461.59627
SGTC_1970st07012235.8 μM0.269841102928TimTec (Natural product derivative library)254.280563.08303TSC3-RPN4
SGTC_1820st05376518.6 μM0.2692313690829TimTec (Natural product derivative library)428.459845.4106RPP1 & pyrimidine depletion