st045864

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1777
Screen concentration 34.8 μM
Source TimTec (Natural product derivative library)
PubChem CID 4691462
SMILES C1C(C2COC(C1=O)O2)N3C4=CC=CC5=C4C(=CC=C5)S3(=O)=O
Standardized SMILES O=C1CC(C2COC1O2)N3c4cccc5cccc(c45)S3(=O)=O
Molecular weight 331.3431
ALogP 1.15
H-bond donor count 0
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.71
% growth inhibition (Hom. pool) -0.01


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4691462
Download HIP data (tab-delimited text)  (excel)
Gene:ASA1(YPR085C)|FD-Score:3.32|P-value:4.43E-4|Clearance:0.12||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:DIB1(YPR082C)|FD-Score:4.05|P-value:2.58E-5|Clearance:0.36||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:ENP2(YGR145W)|FD-Score:3.16|P-value:7.79E-4|Clearance:0.06||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:ERG12(YMR208W)|FD-Score:3.11|P-value:9.49E-4|Clearance:0.21||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ILV5(YLR355C)|FD-Score:3.2|P-value:6.84E-4|Clearance:0.02||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:MPS2(YGL075C)|FD-Score:3.37|P-value:3.81E-4|Clearance:0.04||SGD DESC:Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p Gene:NUP85(YJR042W)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.03||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:POP6(YGR030C)|FD-Score:4.03|P-value:2.84E-5|Clearance:0.36||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRN3(YKL125W)|FD-Score:3.92|P-value:4.41E-5|Clearance:0.36||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:SRP102(YKL154W)|FD-Score:-3.27|P-value:5.44E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:USO1(YDL058W)|FD-Score:3.43|P-value:2.99E-4|Clearance:0.07||SGD DESC:Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex Gene:UTP14(YML093W)|FD-Score:3.18|P-value:7.43E-4|Clearance:0.01||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YJL009W(YJL009W_d)|FD-Score:3.56|P-value:1.88E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YLR230W(YLR230W_d)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:ASA1(YPR085C)|FD-Score:3.32|P-value:4.43E-4|Clearance:0.12||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:DIB1(YPR082C)|FD-Score:4.05|P-value:2.58E-5|Clearance:0.36||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:ENP2(YGR145W)|FD-Score:3.16|P-value:7.79E-4|Clearance:0.06||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:ERG12(YMR208W)|FD-Score:3.11|P-value:9.49E-4|Clearance:0.21||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ILV5(YLR355C)|FD-Score:3.2|P-value:6.84E-4|Clearance:0.02||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:MPS2(YGL075C)|FD-Score:3.37|P-value:3.81E-4|Clearance:0.04||SGD DESC:Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p Gene:NUP85(YJR042W)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.03||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:POP6(YGR030C)|FD-Score:4.03|P-value:2.84E-5|Clearance:0.36||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRN3(YKL125W)|FD-Score:3.92|P-value:4.41E-5|Clearance:0.36||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:SRP102(YKL154W)|FD-Score:-3.27|P-value:5.44E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:USO1(YDL058W)|FD-Score:3.43|P-value:2.99E-4|Clearance:0.07||SGD DESC:Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex Gene:UTP14(YML093W)|FD-Score:3.18|P-value:7.43E-4|Clearance:0.01||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YJL009W(YJL009W_d)|FD-Score:3.56|P-value:1.88E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YLR230W(YLR230W_d)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4691462
Download HOP data (tab-delimited text)  (excel)
Gene:AFR1(YDR085C)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication Gene:ARR2(YPR200C)|FD-Score:-3.78|P-value:7.72E-5||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:BAT2(YJR148W)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase Gene:CDC26(YFR036W)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CLB5(YPR120C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:FMP43(YGR243W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:GDA1(YEL042W)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:IOC3(YFR013W)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:KAP123(YER110C)|FD-Score:-4.07|P-value:2.36E-5||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:LYS4(YDR234W)|FD-Score:5.29|P-value:6.23E-8||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MKS1(YNL076W)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling Gene:MRF1(YGL143C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:NGL3(YML118W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication Gene:NPP2(YEL016C)|FD-Score:4|P-value:3.20E-5||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:PEX12(YMR026C)|FD-Score:3.85|P-value:5.90E-5||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PHO91(YNR013C)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth Gene:PRY1(YJL079C)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:RGI1(YER067W)|FD-Score:-3.11|P-value:9.47E-4||SGD DESC:Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:RPN13(YLR421C)|FD-Score:3.78|P-value:7.89E-5||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress Gene:RPS24B(YIL069C)|FD-Score:-3.73|P-value:9.72E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication Gene:SCS22(YBL091C-A)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication Gene:SNU66(YOR308C)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:SPG1(YGR236C)|FD-Score:4.35|P-value:6.72E-6||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SPO74(YGL170C)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SSK22(YCR073C)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p Gene:TRM3(YDL112W)|FD-Score:4.62|P-value:1.90E-6||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TRM7(YBR061C)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TSC3(YBR058C-A)|FD-Score:6.62|P-value:1.83E-11||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UGX2(YDL169C)|FD-Score:3.74|P-value:9.29E-5||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:VPS13(YLL040C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively Gene:YBT1(YLL048C)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YDR306C(YDR306C_p)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YGL242C(YGL242C_p)|FD-Score:3.76|P-value:8.63E-5||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YHR202W(YHR202W_p)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YIL054W(YIL054W_p)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YLL007C(YLL007C_p)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YMR221C(YMR221C_p)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YPR027C(YPR027C_p)|FD-Score:4.6|P-value:2.08E-6||SGD DESC:Putative protein of unknown function Gene:AFR1(YDR085C)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication Gene:ARR2(YPR200C)|FD-Score:-3.78|P-value:7.72E-5||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:BAT2(YJR148W)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase Gene:CDC26(YFR036W)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CLB5(YPR120C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:FMP43(YGR243W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:GDA1(YEL042W)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:IOC3(YFR013W)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:KAP123(YER110C)|FD-Score:-4.07|P-value:2.36E-5||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:LYS4(YDR234W)|FD-Score:5.29|P-value:6.23E-8||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MKS1(YNL076W)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling Gene:MRF1(YGL143C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:NGL3(YML118W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication Gene:NPP2(YEL016C)|FD-Score:4|P-value:3.20E-5||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:PEX12(YMR026C)|FD-Score:3.85|P-value:5.90E-5||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PHO91(YNR013C)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth Gene:PRY1(YJL079C)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:RGI1(YER067W)|FD-Score:-3.11|P-value:9.47E-4||SGD DESC:Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:RPN13(YLR421C)|FD-Score:3.78|P-value:7.89E-5||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress Gene:RPS24B(YIL069C)|FD-Score:-3.73|P-value:9.72E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication Gene:SCS22(YBL091C-A)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication Gene:SNU66(YOR308C)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:SPG1(YGR236C)|FD-Score:4.35|P-value:6.72E-6||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SPO74(YGL170C)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SSK22(YCR073C)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p Gene:TRM3(YDL112W)|FD-Score:4.62|P-value:1.90E-6||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TRM7(YBR061C)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TSC3(YBR058C-A)|FD-Score:6.62|P-value:1.83E-11||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UGX2(YDL169C)|FD-Score:3.74|P-value:9.29E-5||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:VPS13(YLL040C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively Gene:YBT1(YLL048C)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YDR306C(YDR306C_p)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YGL242C(YGL242C_p)|FD-Score:3.76|P-value:8.63E-5||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YHR202W(YHR202W_p)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YIL054W(YIL054W_p)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YLL007C(YLL007C_p)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YMR221C(YMR221C_p)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YPR027C(YPR027C_p)|FD-Score:4.6|P-value:2.08E-6||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR082C4.052.58E-50.36DIB117-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein
YGR030C4.032.84E-50.36POP6Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YKL125W3.924.41E-50.36RRN3Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
YJL009W_d3.561.88E-40.03YJL009W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex
YLR230W_d3.532.09E-40.06YLR230W_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
YJR042W3.462.67E-40.03NUP85Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75
YDL058W3.432.99E-40.07USO1Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
YGL075C3.373.81E-40.04MPS2Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p
YPR085C3.324.43E-40.12ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
YLR355C3.206.84E-40.02ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YML093W3.187.43E-40.01UTP14Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YGR145W3.167.79E-40.06ENP2Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p
YMR208W3.119.49E-40.21ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YMR079W2.900.001870.01SEC14Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
YIL021W2.890.001930.08RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A6.621.83E-11TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YDR234W5.296.23E-8LYS4Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway
YFR036W5.071.97E-7CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YDL112W4.621.90E-6TRM32'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs
YPR027C_p4.602.08E-6YPR027C_pPutative protein of unknown function
YGR236C4.356.72E-6SPG1Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YDR306C_p4.171.50E-5YDR306C_pF-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain
YNR013C4.171.53E-5PHO91Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth
YEL016C4.003.20E-5NPP2Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication
YMR026C3.855.90E-5PEX12C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder
YLR421C3.787.89E-5RPN13Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress
YGL170C3.778.25E-5SPO74Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation
YGL242C_p3.768.63E-5YGL242C_pPutative protein of unknown function; deletion mutant is viable
YDL169C3.749.29E-5UGX2Protein of unknown function, transcript accumulates in response to any combination of stress conditions
YMR221C_p3.541.97E-4YMR221C_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy

GO enrichment analysis for SGTC_1777
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2251.50E-68SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.056338TSC3-RPN4
0.2054.25E-57SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.106061TSC3-RPN4
0.1983.65E-53SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.15TSC3-RPN4
0.1883.65E-48SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0779221TSC3-RPN4
0.1843.13E-46SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.115942TSC3-RPN4
0.1818.22E-45SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0759494TSC3-RPN4
0.1804.84E-44SGTC_10420350-0096 82.0 μMChemDiv (Drug-like library)31225150.102941TSC3-RPN4
0.1781.81E-43SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0526316TSC3-RPN4
0.1758.21E-42SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0547945TSC3-RPN4
0.1718.60E-40SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.0921053TSC3-RPN4
0.1631.65E-36SGTC_1813st051246 8.6 μMTimTec (Natural product derivative library)52919590.0714286TSC3-RPN4
0.1581.98E-34SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.0795455TSC3-RPN4
0.1575.89E-34SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0555556TSC3-RPN4
0.1571.30E-33SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.0704225
0.1561.67E-33SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.0853659TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1711st03226362.8 μM0.3846153958979TimTec (Natural product derivative library)318.350962.4506
SGTC_1798st04571960.95 μM0.3571433980186TimTec (Natural product derivative library)318.350960.70606
SGTC_1799st04582246.14 μM0.3333333674992TimTec (Natural product derivative library)349.42792.42606
SGTC_1762st04550325.46 μM0.256414645554TimTec (Natural product derivative library)451.247055.30909
SGTC_546peri-Naphthalide147 μM0.22807223824ChemDiv (Drug-like library)184.190722.34702DNA damage response
SGTC_21735763948167 μM0.2063492867177Chembridge (Fragment library)269.29521.81603tubulin folding & SWR complex
SGTC_9741326-0144138 μM0.25411856ChemDiv (Drug-like library)198.22061.96612ERG2
SGTC_216156625048.51 μM0.1935482859334Chembridge (Fragment library)275.68711.50803tubulin folding & SWR complex
SGTC_2214717929748.28 μM0.180328769744Chembridge (Fragment library)235.302121.27603superoxide
SGTC_12720839-002127 μM0.1774195027250ChemDiv (Drug-like library)210.2282.46802