st048256

ethyl 8-methoxy-1,3,4,5-tetrahydropyrido[4,3-b]indole-2-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1793
Screen concentration 72.2 μM
Source TimTec (Natural product derivative library)
PubChem CID 672022
SMILES CCOC(=O)N1CCC2=C(C1)C3=C(N2)C=CC(=C3)OC
Standardized SMILES CCOC(=O)N1CCc2[nH]c3ccc(OC)cc3c2C1
Molecular weight 274.315
ALogP 2.51
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 16.96
% growth inhibition (Hom. pool) 8.06


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 672022
Download HIP data (tab-delimited text)  (excel)
Gene:FOL2(YGR267C)|FD-Score:3.38|P-value:3.61E-4|Clearance:0.22||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:ILV5(YLR355C)|FD-Score:4.93|P-value:4.01E-7|Clearance:0.23||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:MCD4(YKL165C)|FD-Score:3.49|P-value:2.42E-4|Clearance:0.11||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:NUS1(YDL193W)|FD-Score:-3.74|P-value:9.25E-5|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PET9(YBL030C)|FD-Score:-4.52|P-value:3.06E-6|Clearance:0||SGD DESC:Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication Gene:PKC1(YBL105C)|FD-Score:7.7|P-value:6.69E-15|Clearance:2.77||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:QRI1(YDL103C)|FD-Score:3.16|P-value:7.92E-4|Clearance:0.13||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RIM2(YBR192W)|FD-Score:4.59|P-value:2.23E-6|Clearance:0.35||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:TOM40(YMR203W)|FD-Score:4.71|P-value:1.24E-6|Clearance:0.12||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YDL196W(YDL196W_d)|FD-Score:4.24|P-value:1.11E-5|Clearance:0.75||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:FOL2(YGR267C)|FD-Score:3.38|P-value:3.61E-4|Clearance:0.22||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:ILV5(YLR355C)|FD-Score:4.93|P-value:4.01E-7|Clearance:0.23||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:MCD4(YKL165C)|FD-Score:3.49|P-value:2.42E-4|Clearance:0.11||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:NUS1(YDL193W)|FD-Score:-3.74|P-value:9.25E-5|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PET9(YBL030C)|FD-Score:-4.52|P-value:3.06E-6|Clearance:0||SGD DESC:Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication Gene:PKC1(YBL105C)|FD-Score:7.7|P-value:6.69E-15|Clearance:2.77||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:QRI1(YDL103C)|FD-Score:3.16|P-value:7.92E-4|Clearance:0.13||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RIM2(YBR192W)|FD-Score:4.59|P-value:2.23E-6|Clearance:0.35||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:TOM40(YMR203W)|FD-Score:4.71|P-value:1.24E-6|Clearance:0.12||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YDL196W(YDL196W_d)|FD-Score:4.24|P-value:1.11E-5|Clearance:0.75||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 672022
Download HOP data (tab-delimited text)  (excel)
Gene:AIM43(YPL099C)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:BIT2(YBR270C_p)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:DBP1(YPL119C)|FD-Score:3.3|P-value:4.92E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:ELP3(YPL086C)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:EMP70(YLR083C)|FD-Score:-3.51|P-value:2.20E-4||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:ERV41(YML067C)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:IDH2(YOR136W)|FD-Score:-3.33|P-value:4.30E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:MCH2(YKL221W)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MET13(YGL125W)|FD-Score:-3.82|P-value:6.69E-5||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MNN2(YBR015C)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRP17(YKL003C)|FD-Score:-3.09|P-value:9.86E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator Gene:MSN1(YOL116W)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:OST5(YGL226C-A)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PAU7(YAR020C)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PEX12(YMR026C)|FD-Score:-4.19|P-value:1.39E-5||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PLB2(YMR006C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:PTR2(YKR093W)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RMT2(YDR465C)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress Gene:RPN10(YHR200W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RPS17A(YML024W)|FD-Score:-4.04|P-value:2.73E-5||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:-6.04|P-value:7.61E-10||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:SCY1(YGL083W)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase Gene:SPA2(YLL021W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate Gene:SPO74(YGL170C)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:URC2(YDR520C_p)|FD-Score:-4.77|P-value:9.06E-7||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VPS53(YJL029C)|FD-Score:-6.21|P-value:2.59E-10||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting Gene:YBR242W(YBR242W_p)|FD-Score:-3.82|P-value:6.66E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YDR306C(YDR306C_p)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YIG1(YPL201C)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YKL107W(YKL107W_p)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YML034C-A(YML034C-A_d)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ZRT2(YLR130C)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor Gene:AIM43(YPL099C)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:BIT2(YBR270C_p)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:DBP1(YPL119C)|FD-Score:3.3|P-value:4.92E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:ELP3(YPL086C)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:EMP70(YLR083C)|FD-Score:-3.51|P-value:2.20E-4||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:ERV41(YML067C)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:IDH2(YOR136W)|FD-Score:-3.33|P-value:4.30E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:MCH2(YKL221W)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MET13(YGL125W)|FD-Score:-3.82|P-value:6.69E-5||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MNN2(YBR015C)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRP17(YKL003C)|FD-Score:-3.09|P-value:9.86E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator Gene:MSN1(YOL116W)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:OST5(YGL226C-A)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PAU7(YAR020C)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PEX12(YMR026C)|FD-Score:-4.19|P-value:1.39E-5||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PLB2(YMR006C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:PTR2(YKR093W)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RMT2(YDR465C)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress Gene:RPN10(YHR200W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RPS17A(YML024W)|FD-Score:-4.04|P-value:2.73E-5||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:-6.04|P-value:7.61E-10||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:SCY1(YGL083W)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase Gene:SPA2(YLL021W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate Gene:SPO74(YGL170C)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:URC2(YDR520C_p)|FD-Score:-4.77|P-value:9.06E-7||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VPS53(YJL029C)|FD-Score:-6.21|P-value:2.59E-10||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting Gene:YBR242W(YBR242W_p)|FD-Score:-3.82|P-value:6.66E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YDR306C(YDR306C_p)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YIG1(YPL201C)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YKL107W(YKL107W_p)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YML034C-A(YML034C-A_d)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ZRT2(YLR130C)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBL105C7.706.69E-152.77PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YLR355C4.934.01E-70.23ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YMR203W4.711.24E-60.12TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YBR192W4.592.23E-60.35RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YDL196W_d4.241.11E-50.75YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YKL165C3.492.42E-40.11MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YGR267C3.383.61E-40.22FOL2GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
YDL103C3.167.92E-40.13QRI1UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
YIL075C3.030.001210.01RPN2Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress
YLR033W3.020.001270.03RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YBR109C2.990.001390.09CMD1Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
YJL111W2.900.001870.02CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YKL173W2.880.001980.02SNU114GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2
YJR141W_p2.860.002120.01YJR141W_pEssential protein of unknown function
YNL216W2.850.002160.06RAP1Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL099C4.552.69E-6AIM43Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
YAR020C4.241.12E-5PAU7Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme
YPL201C4.022.93E-5YIG1Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol
YKL107W_p4.012.97E-5YKL107W_pPutative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein
YLL021W3.512.24E-4SPA2Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate
YMR006C3.492.44E-4PLB2Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine
YHR200W3.334.31E-4RPN10Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein
YML034C-A_d3.314.68E-4YML034C-A_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGL226C-A3.314.71E-4OST5Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YDR306C_p3.304.82E-4YDR306C_pF-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain
YPL119C3.304.92E-4DBP1Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress
YBR015C3.265.59E-4MNN2Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
YML067C3.245.88E-4ERV41Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein
YOL116W3.216.69E-4MSN1Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress
YLR130C3.206.99E-4ZRT2Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor

GO enrichment analysis for SGTC_1793
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1775.46E-43SGTC_2519cryptotanshinone 88.0 μMMicrosource (Natural product library)1602540.0987654heme biosynthesis & mitochondrial translocase
0.1602.82E-35SGTC_1831st055328 12.0 μMTimTec (Natural product derivative library)11030680.142857heme biosynthesis & mitochondrial translocase
0.1533.49E-32SGTC_7450469-0705 296.0 μMChemDiv (Drug-like library)34867360.166667mitochondrial processes
0.1529.12E-32SGTC_1113artemisinin 1.2 μMTimTec (Natural product library)22400.05heme biosynthesis & mitochondrial translocase
0.1505.91E-31SGTC_14224031-0024 15.5 μMChemDiv (Drug-like library)7824270.105263iron homeostasis
0.1361.38E-25SGTC_31619100388 49.5 μMChembridge (Drug-like library)252363680.0853659iron homeostasis
0.1343.70E-25SGTC_7301436-0052 41.2 μMChemDiv (Drug-like library)7687640.238806mitochondrial processes
0.1344.11E-25SGTC_2510benzyl isothiocyanate 8.3 μMMicrosource (Natural product library)23460.0645161
0.1338.33E-25SGTC_23369001819 198.7 μMChembridge (Fragment library)29896020.136364heme biosynthesis & mitochondrial translocase
0.1292.17E-23SGTC_29309013230 14.9 μMChembridge (Drug-like library)29958570.0769231heme biosynthesis & mitochondrial translocase
0.1248.67E-22SGTC_15526-methylflavone 10.6 μMTimTec (Pure natural product library)6890130.0833333superoxide
0.1232.10E-21SGTC_24755812728 15.0 μMMiscellaneous7761600.121622mitochondrial processes
0.1163.09E-19SGTC_21245246320 200.0 μMChembridge (Fragment library)5793420.0869565heme biosynthesis & mitochondrial translocase
0.1119.33E-18SGTC_623k072-0108 16.9 μMChemDiv (Drug-like library)53828030.0384615heme biosynthesis & mitochondrial translocase
0.1111.00E-17SGTC_7211187-1332 279.0 μMChemDiv (Drug-like library)257300.101449cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1952st07703664.1 μM0.7647062937007TimTec (Natural product derivative library)258.315623.01412
SGTC_1957st07703562.4 μM0.75705593TimTec (Natural product derivative library)278.73413.19212
SGTC_1954st07703268.9 μM0.42623698907TimTec (Natural product derivative library)290.359023.68611
SGTC_2414st07723292.9 μM0.338983729801TimTec (Natural product derivative library)215.247823.13622
SGTC_2696st07769174.32 μM0.338983757133TimTec (Natural product derivative library)200.279542.83611
SGTC_1636st00770768.9 μM0.338462403051TimTec (Natural product derivative library)290.359023.75911
SGTC_13291447-169750.6 μM0.3333332840515ChemDiv (Drug-like library)286.325722.57623
SGTC_2698st07769767.5 μM0.3239443154499TimTec (Natural product derivative library)296.406664.12322
SGTC_1613st00238152.1 μM0.319444551167TimTec (Natural product derivative library)363.45283.83913
SGTC_7734358-145744.6 μM0.3188411186886ChemDiv (Drug-like library)352.38383.73716