st045822

2-(4-prop-2-enyl-5-sulfanylidene-3-thiophen-2-yl-1,2,4-triazol-1-yl)-6,8-dioxabicyclo[3.2.1]octan-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1799
Screen concentration 46.1 μM
Source TimTec (Natural product derivative library)
PubChem CID 3674992
SMILES C=CCN1C(=NN(C1=S)C2CC(=O)C3OCC2O3)C4=CC=CS4
Standardized SMILES C=CCN1C(=S)N(N=C1c2cccs2)C3CC(=O)C4OCC3O4
Molecular weight 349.4279
ALogP 2.43
H-bond donor count 0
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.96
% growth inhibition (Hom. pool) 3.25


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3674992
Download HIP data (tab-delimited text)  (excel)
Gene:CDC24(YAL041W)|FD-Score:3.99|P-value:3.25E-5|Clearance:0.14||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:FRS1(YLR060W)|FD-Score:3.2|P-value:6.87E-4|Clearance:0.13||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:GPI18(YBR004C)|FD-Score:5.76|P-value:4.30E-9|Clearance:1.76||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:KAR1(YNL188W)|FD-Score:-3.43|P-value:3.05E-4|Clearance:0||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:PDS5(YMR076C)|FD-Score:-3.73|P-value:9.67E-5|Clearance:0||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PET9(YBL030C)|FD-Score:-4.14|P-value:1.72E-5|Clearance:0||SGD DESC:Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication Gene:RPF1(YHR088W)|FD-Score:3.33|P-value:4.29E-4|Clearance:0.13||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:SAM50(YNL026W)|FD-Score:-3.46|P-value:2.75E-4|Clearance:0||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SCL1(YGL011C)|FD-Score:-3.22|P-value:6.42E-4|Clearance:0||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC61(YLR378C)|FD-Score:-4.88|P-value:5.20E-7|Clearance:0||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:SOF1(YLL011W)|FD-Score:3.85|P-value:5.88E-5|Clearance:0.01||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:SPP41(YDR464W)|FD-Score:-3.63|P-value:1.42E-4|Clearance:0||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:TAF5(YBR198C)|FD-Score:3.84|P-value:6.17E-5|Clearance:0.51||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:CDC24(YAL041W)|FD-Score:3.99|P-value:3.25E-5|Clearance:0.14||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:FRS1(YLR060W)|FD-Score:3.2|P-value:6.87E-4|Clearance:0.13||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:GPI18(YBR004C)|FD-Score:5.76|P-value:4.30E-9|Clearance:1.76||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:KAR1(YNL188W)|FD-Score:-3.43|P-value:3.05E-4|Clearance:0||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:PDS5(YMR076C)|FD-Score:-3.73|P-value:9.67E-5|Clearance:0||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PET9(YBL030C)|FD-Score:-4.14|P-value:1.72E-5|Clearance:0||SGD DESC:Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication Gene:RPF1(YHR088W)|FD-Score:3.33|P-value:4.29E-4|Clearance:0.13||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:SAM50(YNL026W)|FD-Score:-3.46|P-value:2.75E-4|Clearance:0||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SCL1(YGL011C)|FD-Score:-3.22|P-value:6.42E-4|Clearance:0||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC61(YLR378C)|FD-Score:-4.88|P-value:5.20E-7|Clearance:0||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:SOF1(YLL011W)|FD-Score:3.85|P-value:5.88E-5|Clearance:0.01||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:SPP41(YDR464W)|FD-Score:-3.63|P-value:1.42E-4|Clearance:0||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:TAF5(YBR198C)|FD-Score:3.84|P-value:6.17E-5|Clearance:0.51||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3674992
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:BFR1(YOR198C)|FD-Score:-3.12|P-value:9.14E-4||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BNA1(YJR025C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:CHS3(YBR023C)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CPR7(YJR032W)|FD-Score:4.42|P-value:4.98E-6||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CRG1(YHR209W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:CSF1(YLR087C)|FD-Score:-3.78|P-value:7.92E-5||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSI2(YOL007C)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene Gene:CYB2(YML054C)|FD-Score:-4.18|P-value:1.48E-5||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:DAP1(YPL170W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DSE2(YHR143W)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:EAF5(YEL018W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:ENT2(YLR206W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus Gene:ERG4(YGL012W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FAT1(YBR041W)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FLR1(YBR008C)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:GRE1(YPL223C)|FD-Score:-4.3|P-value:8.56E-6||SGD DESC:Hydrophilin essential in dessication-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway Gene:GRE3(YHR104W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:GTT2(YLL060C)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HCM1(YCR065W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role Gene:HNT1(YDL125C)|FD-Score:-4.85|P-value:6.03E-7||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HSP26(YBR072W)|FD-Score:3.88|P-value:5.30E-5||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:HVG1(YER039C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:IKI3(YLR384C)|FD-Score:-3.94|P-value:4.00E-5||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:KTI11(YBL071W-A)|FD-Score:-3.15|P-value:8.08E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:MAL32(YBR299W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MCX1(YBR227C)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MDV1(YJL112W)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:MSH2(YOL090W)|FD-Score:-3.74|P-value:9.09E-5||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MZM1(YDR493W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NUP53(YMR153W)|FD-Score:4.46|P-value:4.19E-6||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:OYE2(YHR179W)|FD-Score:4.46|P-value:4.05E-6||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PEA2(YER149C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth Gene:PEP4(YPL154C)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PEX12(YMR026C)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PGM1(YKL127W)|FD-Score:4.36|P-value:6.45E-6||SGD DESC:Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism Gene:PKH1(YDR490C)|FD-Score:6.09|P-value:5.82E-10||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:POP2(YNR052C)|FD-Score:4.46|P-value:4.10E-6||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PUS7(YOR243C)|FD-Score:5.38|P-value:3.65E-8||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:QCR8(YJL166W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p Gene:QDR3(YBR043C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:RDL2(YOR286W)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss Gene:RNH1(YMR234W)|FD-Score:3.8|P-value:7.17E-5||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:RPL16B(YNL069C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RSC1(YGR056W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RTG2(YGL252C)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:SDT1(YGL224C)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SLY41(YOR307C)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:Protein involved in ER-to-Golgi transport Gene:SOL3(YHR163W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:TPO4(YOR273C)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRM7(YBR061C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:URC2(YDR520C_p)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VID30(YGL227W)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:YAR030C(YAR030C_d)|FD-Score:4.54|P-value:2.82E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 Gene:YCR061W(YCR061W_p)|FD-Score:-3.19|P-value:7.13E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YIL134C-A(YIL134C-A_p)|FD-Score:3.99|P-value:3.26E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIR020W-A(YIR020W-A_d)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YLR419W(YLR419W_p)|FD-Score:4.02|P-value:2.97E-5||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YMR304C-A(YMR304C-A_d)|FD-Score:5.48|P-value:2.15E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YMR316C-A(YMR316C-A_p)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YOR139C(YOR139C_d)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YOR343C(YOR343C_d)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR385W(YOR385W_p)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL038W-A(YPL038W-A_p)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YSP2(YDR326C)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:ACE2(YLR131C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:BFR1(YOR198C)|FD-Score:-3.12|P-value:9.14E-4||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BNA1(YJR025C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:CHS3(YBR023C)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CPR7(YJR032W)|FD-Score:4.42|P-value:4.98E-6||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CRG1(YHR209W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:CSF1(YLR087C)|FD-Score:-3.78|P-value:7.92E-5||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSI2(YOL007C)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene Gene:CYB2(YML054C)|FD-Score:-4.18|P-value:1.48E-5||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:DAP1(YPL170W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DSE2(YHR143W)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:EAF5(YEL018W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:ENT2(YLR206W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus Gene:ERG4(YGL012W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FAT1(YBR041W)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FLR1(YBR008C)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:GRE1(YPL223C)|FD-Score:-4.3|P-value:8.56E-6||SGD DESC:Hydrophilin essential in dessication-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway Gene:GRE3(YHR104W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:GTT2(YLL060C)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HCM1(YCR065W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role Gene:HNT1(YDL125C)|FD-Score:-4.85|P-value:6.03E-7||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HSP26(YBR072W)|FD-Score:3.88|P-value:5.30E-5||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:HVG1(YER039C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:IKI3(YLR384C)|FD-Score:-3.94|P-value:4.00E-5||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:KTI11(YBL071W-A)|FD-Score:-3.15|P-value:8.08E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:MAL32(YBR299W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MCX1(YBR227C)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MDV1(YJL112W)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:MSH2(YOL090W)|FD-Score:-3.74|P-value:9.09E-5||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MZM1(YDR493W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NUP53(YMR153W)|FD-Score:4.46|P-value:4.19E-6||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:OYE2(YHR179W)|FD-Score:4.46|P-value:4.05E-6||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PEA2(YER149C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth Gene:PEP4(YPL154C)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PEX12(YMR026C)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PGM1(YKL127W)|FD-Score:4.36|P-value:6.45E-6||SGD DESC:Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism Gene:PKH1(YDR490C)|FD-Score:6.09|P-value:5.82E-10||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:POP2(YNR052C)|FD-Score:4.46|P-value:4.10E-6||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PUS7(YOR243C)|FD-Score:5.38|P-value:3.65E-8||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:QCR8(YJL166W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p Gene:QDR3(YBR043C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:RDL2(YOR286W)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss Gene:RNH1(YMR234W)|FD-Score:3.8|P-value:7.17E-5||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:RPL16B(YNL069C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RSC1(YGR056W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RTG2(YGL252C)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:SDT1(YGL224C)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SLY41(YOR307C)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:Protein involved in ER-to-Golgi transport Gene:SOL3(YHR163W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:TPO4(YOR273C)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRM7(YBR061C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:URC2(YDR520C_p)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VID30(YGL227W)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:YAR030C(YAR030C_d)|FD-Score:4.54|P-value:2.82E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 Gene:YCR061W(YCR061W_p)|FD-Score:-3.19|P-value:7.13E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YIL134C-A(YIL134C-A_p)|FD-Score:3.99|P-value:3.26E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIR020W-A(YIR020W-A_d)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YLR419W(YLR419W_p)|FD-Score:4.02|P-value:2.97E-5||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YMR304C-A(YMR304C-A_d)|FD-Score:5.48|P-value:2.15E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YMR316C-A(YMR316C-A_p)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YOR139C(YOR139C_d)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YOR343C(YOR343C_d)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR385W(YOR385W_p)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL038W-A(YPL038W-A_p)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YSP2(YDR326C)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR004C5.764.30E-91.76GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YAL041W3.993.25E-50.14CDC24Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress
YLL011W3.855.88E-50.01SOF1Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
YBR198C3.846.17E-50.51TAF5Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YHR088W3.334.29E-40.13RPF1Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA
YLR060W3.206.87E-40.13FRS1Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
YAR019C3.070.001070.00CDC15Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress
YOR046C3.070.001080.24DBP5Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p
YMR047C2.830.002360.05NUP116FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p)
YHR170W2.770.002780.07NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YKL089W2.700.003500.09MIF2Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3
YMR296C2.610.004510.00LCB1Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YLL004W2.610.004540.05ORC3Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YJL203W2.560.005260.02PRP21Subunit of the SF3a splicing factor complex, required for spliceosome assembly
YGR083C2.540.005520.04GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR490C6.095.82E-10PKH1Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p
YMR304C-A_d5.482.15E-8YMR304C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10
YOR243C5.383.65E-8PUS7Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria
YAR030C_d4.542.82E-6YAR030C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9
YHR179W4.464.05E-6OYE2Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress
YNR052C4.464.10E-6POP2RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation
YMR153W4.464.19E-6NUP53FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication
YJR032W4.424.98E-6CPR7Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity
YHR143W4.385.88E-6DSE2Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP
YKL127W4.366.45E-6PGM1Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism
YGL012W4.161.61E-5ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YJL166W4.141.77E-5QCR8Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
YLR419W_p4.022.97E-5YLR419W_pPutative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene
YIL134C-A_p3.993.26E-5YIL134C-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YBR072W3.885.30E-5HSP26Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity

GO enrichment analysis for SGTC_1799
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0757.48E-9SGTC_712r015-0009 80.6 μMChemDiv (Drug-like library)68138720.0740741
0.0672.97E-7SGTC_13221348-1070 12.0 μMChemDiv (Drug-like library)67580890.0612245
0.0604.00E-6SGTC_29789011784 43.0 μMChembridge (Drug-like library)29949340.0689655
0.0594.78E-6SGTC_12480469-0687 58.6 μMChemDiv (Drug-like library)40174820.0941176
0.0571.14E-5SGTC_1503970-0969 77.2 μMChemDiv (Drug-like library)66156400.0754717
0.0571.17E-5SGTC_1793st048256 72.2 μMTimTec (Natural product derivative library)6720220.0736842
0.0571.19E-5SGTC_1790st045711 43.5 μMTimTec (Natural product derivative library)5851190.0594059
0.0571.25E-5SGTC_466geldanamycin 89.2 μMICCB bioactive library52883820.0583333RPP1 & pyrimidine depletion
0.0552.49E-5SGTC_13641653-0028 37.6 μMChemDiv (Drug-like library)57186520.106796
0.0543.10E-5SGTC_33259138760 55.2 μMChembridge (Drug-like library)438449590.06Golgi
0.0543.22E-5SGTC_13301469-0054 61.8 μMChemDiv (Drug-like library)71026640.0673077iron homeostasis
0.0543.30E-5SGTC_1768st044301 37.2 μMTimTec (Natural product derivative library)39313490.106796
0.0533.95E-5SGTC_1073triclabendazole 3.1 μMNIH Clinical Collection502480.0549451
0.0534.17E-5SGTC_2489cantharidin 73.4 μMICCB bioactive library25450.1cell wall signaling
0.0534.47E-5SGTC_608k048-0125 159.0 μMChemDiv (Drug-like library)68176520.0638298

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1798st04571960.95 μM0.4861113980186TimTec (Natural product derivative library)318.350960.70606
SGTC_1711st03226362.8 μM0.4571433958979TimTec (Natural product derivative library)318.350962.4506
SGTC_1777st04586434.77 μM0.3333334691462TimTec (Natural product derivative library)331.343121.1506
SGTC_2442515890648.48 μM0.251893835Miscellaneous251.324663.05904
SGTC_12103013-0140750 nM0.236842905973ChemDiv (Drug-like library)275.286461.18404
SGTC_1762st04550325.46 μM0.2359554645554TimTec (Natural product derivative library)451.247055.30909
SGTC_3354077-025627.6 μM0.226193121989ChemDiv (Drug-like library)363.27213.69404
SGTC_1534077-0274519.67 μM0.2134833121976ChemDiv (Drug-like library)384.8543635.49905
SGTC_10483161-030024.5 μM0.2054792165101ChemDiv (Drug-like library)266.090722.24102
SGTC_2032518511225.99 μM0.1973685718189Chembridge (Fragment library)241.286781.54415