st050076

6,7-diethoxy-1-(4-fluoro-3-phenoxyphenyl)-1,2,3,4-tetrahydroisoquinoline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1804
Screen concentration 49.1 μM
Source TimTec (Natural product derivative library)
PubChem CID 3895976
SMILES CCOC1=C(C=C2C(NCCC2=C1)C3=CC(=C(C=C3)F)OC4=CC=CC=C4)OCC
Standardized SMILES CCOc1cc2CCNC(c3ccc(F)c(Oc4ccccc4)c3)c2cc1OCC
Molecular weight 407.4772
ALogP 5.49
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 26.22
% growth inhibition (Hom. pool) 9.16


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3895976
Download HIP data (tab-delimited text)  (excel)
Gene:CCT4(YDL143W)|FD-Score:3.81|P-value:6.95E-5|Clearance:0.01||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.16||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:MAS1(YLR163C)|FD-Score:3.09|P-value:9.98E-4|Clearance:0.04||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NUP49(YGL172W)|FD-Score:3.14|P-value:8.51E-4|Clearance:0.01||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PIK1(YNL267W)|FD-Score:5.24|P-value:7.99E-8|Clearance:0.72||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:REB1(YBR049C)|FD-Score:3.13|P-value:8.80E-4|Clearance:0.02||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RPC19(YNL113W)|FD-Score:3.1|P-value:9.52E-4|Clearance:0.01||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPL25(YOL127W)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPS20(YHL015W)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.32||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.22||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:TCP1(YDR212W)|FD-Score:3.8|P-value:7.11E-5|Clearance:0.01||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:UTP4(YDR324C)|FD-Score:3.79|P-value:7.49E-5|Clearance:0.09||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YDR187C(YDR187C_d)|FD-Score:3.13|P-value:8.76E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YJL009W(YJL009W_d)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YRB1(YDR002W)|FD-Score:4.85|P-value:6.25E-7|Clearance:0.72||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 Gene:CCT4(YDL143W)|FD-Score:3.81|P-value:6.95E-5|Clearance:0.01||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.16||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:MAS1(YLR163C)|FD-Score:3.09|P-value:9.98E-4|Clearance:0.04||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NUP49(YGL172W)|FD-Score:3.14|P-value:8.51E-4|Clearance:0.01||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PIK1(YNL267W)|FD-Score:5.24|P-value:7.99E-8|Clearance:0.72||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:REB1(YBR049C)|FD-Score:3.13|P-value:8.80E-4|Clearance:0.02||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RPC19(YNL113W)|FD-Score:3.1|P-value:9.52E-4|Clearance:0.01||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPL25(YOL127W)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPS20(YHL015W)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.32||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.22||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:TCP1(YDR212W)|FD-Score:3.8|P-value:7.11E-5|Clearance:0.01||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:UTP4(YDR324C)|FD-Score:3.79|P-value:7.49E-5|Clearance:0.09||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YDR187C(YDR187C_d)|FD-Score:3.13|P-value:8.76E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YJL009W(YJL009W_d)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YRB1(YDR002W)|FD-Score:4.85|P-value:6.25E-7|Clearance:0.72||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3895976
Download HOP data (tab-delimited text)  (excel)
Gene:ADD66(YKL206C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:AHC2(YCR082W)|FD-Score:-3.76|P-value:8.46E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:AIM18(YHR198C)|FD-Score:4.32|P-value:7.74E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALK2(YBL009W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:CCC2(YDR270W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CKB1(YGL019W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CKB2(YOR039W)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CUE5(YOR042W)|FD-Score:-4.46|P-value:4.05E-6||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DFG5(YMR238W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DPP1(YDR284C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:ECL1(YGR146C)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:ECM33(YBR078W)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ELP6(YMR312W)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FZF1(YGL254W)|FD-Score:-3.15|P-value:8.15E-4||SGD DESC:Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress Gene:GAL11(YOL051W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GAL7(YBR018C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GIM5(YML094W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GIP1(YBR045C)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GIS3(YLR094C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Protein of unknown function Gene:IKI1(YHR187W)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IRC21(YMR073C)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRC9(YJL142C_d)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YJL141C; null mutant displays increased levels of spontaneous Rad52p foci Gene:LEM3(YNL323W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LEO1(YOR123C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LSM1(YJL124C)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MID2(YLR332W)|FD-Score:4.98|P-value:3.16E-7||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:NIP100(YPL174C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NUP60(YAR002W)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PEX34(YCL056C)|FD-Score:3.77|P-value:8.18E-5||SGD DESC:Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation Gene:RFM1(YOR279C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RRD1(YIL153W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SIP3(YNL257C)|FD-Score:-3.93|P-value:4.27E-5||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SSA3(YBL075C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:STP22(YCL008C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:TAN1(YGL232W)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:THP1(YOL072W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TIR3(YIL011W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TVP23(YDR084C)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBP6(YFR010W)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VPS20(YMR077C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS28(YPL065W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:5.36|P-value:4.18E-8||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YAL045C(YAL045C_d)|FD-Score:3.76|P-value:8.46E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A Gene:YAP1802(YGR241C)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YCL002C(YCL002C_p)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR085W(YCR085W_d)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCT1(YLL055W)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene Gene:YDL009C(YDL009C_p)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YER039C-A(YER039C-A_p)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YER119C-A(YER119C-A_d)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGR139W(YGR139W_d)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR168C(YGR168C_p)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YHR022C(YHR022C_p)|FD-Score:-4.5|P-value:3.47E-6||SGD DESC:Putative protein of unknown function; YHR022C is not an essential gene Gene:YHR202W(YHR202W_p)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YIL024C(YIL024C_p)|FD-Score:-3.99|P-value:3.27E-5||SGD DESC:Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p Gene:YJR096W(YJR096W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YKR047W(YKR047W_d)|FD-Score:5.14|P-value:1.40E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR444C(YLR444C_d)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR075C-A(YMR075C-A_d)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YMR245W(YMR245W_d)|FD-Score:5.24|P-value:7.89E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL203C(YNL203C_d)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR105W(YOR105W_p)|FD-Score:4.33|P-value:7.60E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR366W(YOR366W_d)|FD-Score:4.27|P-value:9.56E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YPK1(YKL126W)|FD-Score:5.01|P-value:2.72E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ADD66(YKL206C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:AHC2(YCR082W)|FD-Score:-3.76|P-value:8.46E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:AIM18(YHR198C)|FD-Score:4.32|P-value:7.74E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALK2(YBL009W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:CCC2(YDR270W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CKB1(YGL019W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CKB2(YOR039W)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CUE5(YOR042W)|FD-Score:-4.46|P-value:4.05E-6||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DFG5(YMR238W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DPP1(YDR284C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:ECL1(YGR146C)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:ECM33(YBR078W)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ELP6(YMR312W)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FZF1(YGL254W)|FD-Score:-3.15|P-value:8.15E-4||SGD DESC:Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress Gene:GAL11(YOL051W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GAL7(YBR018C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GIM5(YML094W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GIP1(YBR045C)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GIS3(YLR094C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Protein of unknown function Gene:IKI1(YHR187W)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IRC21(YMR073C)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRC9(YJL142C_d)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YJL141C; null mutant displays increased levels of spontaneous Rad52p foci Gene:LEM3(YNL323W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LEO1(YOR123C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LSM1(YJL124C)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MID2(YLR332W)|FD-Score:4.98|P-value:3.16E-7||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:NIP100(YPL174C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NUP60(YAR002W)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PEX34(YCL056C)|FD-Score:3.77|P-value:8.18E-5||SGD DESC:Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation Gene:RFM1(YOR279C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RRD1(YIL153W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SIP3(YNL257C)|FD-Score:-3.93|P-value:4.27E-5||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SSA3(YBL075C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:STP22(YCL008C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:TAN1(YGL232W)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:THP1(YOL072W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TIR3(YIL011W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TVP23(YDR084C)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBP6(YFR010W)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VPS20(YMR077C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS28(YPL065W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:5.36|P-value:4.18E-8||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YAL045C(YAL045C_d)|FD-Score:3.76|P-value:8.46E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A Gene:YAP1802(YGR241C)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YCL002C(YCL002C_p)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR085W(YCR085W_d)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCT1(YLL055W)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene Gene:YDL009C(YDL009C_p)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YER039C-A(YER039C-A_p)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YER119C-A(YER119C-A_d)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGR139W(YGR139W_d)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR168C(YGR168C_p)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YHR022C(YHR022C_p)|FD-Score:-4.5|P-value:3.47E-6||SGD DESC:Putative protein of unknown function; YHR022C is not an essential gene Gene:YHR202W(YHR202W_p)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YIL024C(YIL024C_p)|FD-Score:-3.99|P-value:3.27E-5||SGD DESC:Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p Gene:YJR096W(YJR096W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YKR047W(YKR047W_d)|FD-Score:5.14|P-value:1.40E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR444C(YLR444C_d)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR075C-A(YMR075C-A_d)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YMR245W(YMR245W_d)|FD-Score:5.24|P-value:7.89E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL203C(YNL203C_d)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR105W(YOR105W_p)|FD-Score:4.33|P-value:7.60E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR366W(YOR366W_d)|FD-Score:4.27|P-value:9.56E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YPK1(YKL126W)|FD-Score:5.01|P-value:2.72E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL267W5.247.99E-80.72PIK1Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
YDR002W4.856.25E-70.72YRB1Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1
YHL015W4.131.83E-50.32RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
YDL143W3.816.95E-50.01CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YDR212W3.807.11E-50.01TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YDR324C3.797.49E-50.09UTP4Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript
YNL178W3.701.07E-40.22RPS3Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3
YOL127W3.482.50E-40.06RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YJR064W3.423.14E-40.16CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YJL009W_d3.265.52E-40.12YJL009W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex
YGL172W3.148.51E-40.01NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YDR187C_d3.138.76E-40.00YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YBR049C3.138.80E-40.02REB1RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
YNL113W3.109.52E-40.01RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YLR163C3.099.98E-40.04MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR417W5.364.18E-8VPS36Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
YMR245W_d5.247.89E-8YMR245W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YKR047W_d5.141.40E-7YKR047W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1
YKL126W5.012.72E-7YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YLR332W4.983.16E-7MID2O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
YHR202W_p4.641.78E-6YHR202W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization
YOR105W_p4.337.60E-6YOR105W_pProtein of unknown function; expressed at both mRNA and protein levels
YHR198C4.327.74E-6AIM18Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
YOR366W_d4.279.56E-6YOR366W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C
YCR085W_d4.251.06E-5YCR085W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPL174C4.221.23E-5NIP100Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued)
YCL008C4.211.29E-5STP22Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype
YDR084C4.201.36E-5TVP23Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YPL065W4.042.70E-5VPS28Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
YBR078W4.022.95E-5ECM33GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p

GO enrichment analysis for SGTC_1804
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1583.02E-34SGTC_3133474-0040 5.0 μMChemDiv (Drug-like library)10433750.0657895NEO1-PIK1
0.1402.49E-27SGTC_1672st016598 6.5 μMTimTec (Natural product derivative library)46658600.101124endomembrane recycling
0.1376.13E-26SGTC_1809st050081 57.6 μMTimTec (Natural product derivative library)50368940.654545
0.1352.90E-25SGTC_1760st045453 10.4 μMTimTec (Natural product derivative library)33234190.610169NEO1-PIK1
0.1257.44E-22SGTC_1801st050089 28.4 μMTimTec (Natural product derivative library)50084930.740741NEO1-PIK1
0.1095.20E-17SGTC_20064011487 200.0 μMChembridge (Fragment library)455952940.0735294endomembrane recycling
0.1031.99E-15SGTC_1593justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 54.9 μMTimTec (Pure natural product library)4428820.107143redox potentiating
0.1001.61E-14SGTC_221trifluoperazine 10.3 μMMiscellaneous55660.119048NEO1-PIK1
0.0992.00E-14SGTC_1803st050068 52.4 μMTimTec (Natural product derivative library)39840590.603448NEO1-PIK1
0.0976.64E-14SGTC_1545lovastatin 49.4 μMTimTec (Pure natural product library)129236120.0792079
0.0961.54E-13SGTC_1463448-8650 53.1 μMChemDiv (Drug-like library)28914880.0744681NEO1-PIK1
0.0961.84E-13SGTC_1687st020778 55.4 μMTimTec (Natural product derivative library)42592990.164706
0.0944.18E-13SGTC_1754st042065 16.1 μMTimTec (Natural product derivative library)11262360.0921053NEO1-PIK1
0.0911.95E-12SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.123457ERG2
0.0904.58E-12SGTC_3014119-0100 24.5 μMChemDiv (Drug-like library)28010.144737NEO1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1802st05011257.9 μM0.7547174662563TimTec (Natural product derivative library)345.4078633.90815
SGTC_1801st05008928.44 μM0.7407415008493TimTec (Natural product derivative library)389.486785.27914NEO1-PIK1
SGTC_1800st05007929.07 μM0.6666673645560TimTec (Natural product derivative library)373.487385.23713NEO1-PIK1
SGTC_1809st05008157.6 μM0.6545455036894TimTec (Natural product derivative library)347.45014.62713
SGTC_1760st04545310.45 μM0.6101693323419TimTec (Natural product derivative library)385.498085.38213NEO1-PIK1
SGTC_1803st05006852.4 μM0.6034483984059TimTec (Natural product derivative library)381.388795.83917NEO1-PIK1
SGTC_1806st05000560.3 μM0.65194765TimTec (Natural product derivative library)331.836484.38313
SGTC_1808st05006361.5 μM0.64011993TimTec (Natural product derivative library)325.444584.66113
SGTC_1805st05001360.3 μM0.5964914037144TimTec (Natural product derivative library)331.836484.38313
SGTC_1810st05013853 μM0.524593444541TimTec (Natural product derivative library)377.476084.61114PDR1