st050063

6,7-diethoxy-1-(4-ethylphenyl)-1,2,3,4-tetrahydroisoquinoline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1808
Screen concentration 61.5 μM
Source TimTec (Natural product derivative library)
PubChem CID 4011993
SMILES CCC1=CC=C(C=C1)C2C3=CC(=C(C=C3CCN2)OCC)OCC
Standardized SMILES CCOc1cc2CCNC(c3ccc(CC)cc3)c2cc1OCC
Molecular weight 325.4446
ALogP 4.66
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 16.38
% growth inhibition (Hom. pool) 8.24


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4011993
Download HIP data (tab-delimited text)  (excel)
Gene:ERG1(YGR175C)|FD-Score:-3.54|P-value:2.01E-4|Clearance:0||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:MAS1(YLR163C)|FD-Score:3.16|P-value:7.86E-4|Clearance:0.08||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:RAM2(YKL019W)|FD-Score:3.49|P-value:2.42E-4|Clearance:0.19||SGD DESC:Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor Gene:RPP1(YHR062C)|FD-Score:-3.33|P-value:4.32E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSC58(YLR033W)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.14||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:TIM10(YHR005C-A)|FD-Score:-3.95|P-value:3.88E-5|Clearance:0||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:YGR114C(YGR114C_d)|FD-Score:4.09|P-value:2.18E-5|Clearance:0.6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:ERG1(YGR175C)|FD-Score:-3.54|P-value:2.01E-4|Clearance:0||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:MAS1(YLR163C)|FD-Score:3.16|P-value:7.86E-4|Clearance:0.08||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:RAM2(YKL019W)|FD-Score:3.49|P-value:2.42E-4|Clearance:0.19||SGD DESC:Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor Gene:RPP1(YHR062C)|FD-Score:-3.33|P-value:4.32E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSC58(YLR033W)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.14||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:TIM10(YHR005C-A)|FD-Score:-3.95|P-value:3.88E-5|Clearance:0||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:YGR114C(YGR114C_d)|FD-Score:4.09|P-value:2.18E-5|Clearance:0.6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4011993
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:4.65|P-value:1.67E-6||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AEP2(YMR282C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM37(YNL100W)|FD-Score:4.77|P-value:9.13E-7||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:APL1(YJR005W)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ASM4(YDL088C)|FD-Score:4.81|P-value:7.52E-7||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:ATC1(YDR184C)|FD-Score:-3.89|P-value:4.91E-5||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:ATP5(YDR298C)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BRE2(YLR015W)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L Gene:BTS1(YPL069C)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAN1(YEL063C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CDC55(YGL190C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CPR5(YDR304C)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:CUE5(YOR042W)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DAL80(YKR034W)|FD-Score:3.31|P-value:4.58E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DGR2(YKL121W_p)|FD-Score:4.44|P-value:4.59E-6||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:ECM38(YLR299W)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation Gene:EHD3(YDR036C)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis Gene:FMO1(YHR176W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FSH3(YOR280C)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:FUI1(YBL042C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GDT1(YBR187W_p)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:HAL1(YPR005C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:IRC9(YJL142C_d)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YJL141C; null mutant displays increased levels of spontaneous Rad52p foci Gene:KGD1(YIL125W)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA Gene:LYP1(YNL268W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MDY2(YOL111C)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MID2(YLR332W)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MRPL25(YGR076C)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:MSB1(YOR188W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSN5(YDR335W)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MSO1(YNR049C)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:MZM1(YDR493W)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NTG2(YOL043C)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication Gene:PCI8(YIL071C)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PRM5(YIL117C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PRY1(YJL079C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:RFM1(YOR279C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGP1(YDR137W)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL31A(YDL075W)|FD-Score:4.27|P-value:9.77E-6||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPN13(YLR421C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress Gene:RPP1B(YDL130W)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS22A(YJL190C)|FD-Score:-3.83|P-value:6.51E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RTN2(YDL204W)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SIP4(YJL089W)|FD-Score:-3.35|P-value:4.08E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SKG1(YKR100C)|FD-Score:-3.52|P-value:2.16E-4||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SRO7(YPR032W)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:SSN3(YPL042C)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STO1(YMR125W)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SWI4(YER111C)|FD-Score:-3.22|P-value:6.30E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:THI2(YBR240C)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:TPN1(YGL186C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:UBC4(YBR082C)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication Gene:UBP2(YOR124C)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:UFD2(YDL190C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:VMA13(YPR036W)|FD-Score:4.98|P-value:3.26E-7||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS51(YKR020W)|FD-Score:-3.29|P-value:4.93E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:YBR134W(YBR134W_d)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YCR025C(YCR025C_d)|FD-Score:3.91|P-value:4.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YDR445C(YDR445C_d)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR514C(YDR514C)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YGL177W(YGL177W_d)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL046W-A(YIL046W-A_p)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YKL027W(YKL027W)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPR089W(YPR089W_p)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:ACB1(YGR037C)|FD-Score:4.65|P-value:1.67E-6||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AEP2(YMR282C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM37(YNL100W)|FD-Score:4.77|P-value:9.13E-7||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:APL1(YJR005W)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ASM4(YDL088C)|FD-Score:4.81|P-value:7.52E-7||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:ATC1(YDR184C)|FD-Score:-3.89|P-value:4.91E-5||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:ATP5(YDR298C)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BRE2(YLR015W)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L Gene:BTS1(YPL069C)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAN1(YEL063C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CDC55(YGL190C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CPR5(YDR304C)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:CUE5(YOR042W)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DAL80(YKR034W)|FD-Score:3.31|P-value:4.58E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DGR2(YKL121W_p)|FD-Score:4.44|P-value:4.59E-6||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:ECM38(YLR299W)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation Gene:EHD3(YDR036C)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis Gene:FMO1(YHR176W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FSH3(YOR280C)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:FUI1(YBL042C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GDT1(YBR187W_p)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:HAL1(YPR005C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:IRC9(YJL142C_d)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YJL141C; null mutant displays increased levels of spontaneous Rad52p foci Gene:KGD1(YIL125W)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA Gene:LYP1(YNL268W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MDY2(YOL111C)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MID2(YLR332W)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MRPL25(YGR076C)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:MSB1(YOR188W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSN5(YDR335W)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MSO1(YNR049C)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:MZM1(YDR493W)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NTG2(YOL043C)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication Gene:PCI8(YIL071C)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PRM5(YIL117C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PRY1(YJL079C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:RFM1(YOR279C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGP1(YDR137W)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL31A(YDL075W)|FD-Score:4.27|P-value:9.77E-6||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPN13(YLR421C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress Gene:RPP1B(YDL130W)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS22A(YJL190C)|FD-Score:-3.83|P-value:6.51E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RTN2(YDL204W)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SIP4(YJL089W)|FD-Score:-3.35|P-value:4.08E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SKG1(YKR100C)|FD-Score:-3.52|P-value:2.16E-4||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SRO7(YPR032W)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:SSN3(YPL042C)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STO1(YMR125W)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SWI4(YER111C)|FD-Score:-3.22|P-value:6.30E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:THI2(YBR240C)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:TPN1(YGL186C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:UBC4(YBR082C)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication Gene:UBP2(YOR124C)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:UFD2(YDL190C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:VMA13(YPR036W)|FD-Score:4.98|P-value:3.26E-7||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS51(YKR020W)|FD-Score:-3.29|P-value:4.93E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:YBR134W(YBR134W_d)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YCR025C(YCR025C_d)|FD-Score:3.91|P-value:4.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YDR445C(YDR445C_d)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR514C(YDR514C)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YGL177W(YGL177W_d)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL046W-A(YIL046W-A_p)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YKL027W(YKL027W)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPR089W(YPR089W_p)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR114C_d4.092.18E-50.60YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YKL019W3.492.42E-40.19RAM2Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor
YLR033W3.304.83E-40.14RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YLR163C3.167.86E-40.08MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YKL195W3.080.001030.02MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YGL099W3.060.001110.09LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YBR143C2.970.001510.00SUP45Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p
YPL028W2.960.001520.01ERG10Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis
YOR095C2.950.001580.10RKI1Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
YGL069C_d2.850.002160.02YGL069C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance
YFL009W2.840.002280.04CDC4F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p
YGL048C2.800.002550.09RPT6ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
YLR197W2.710.003400.11NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YDR404C2.600.004690.03RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YHR036W2.570.005070.05BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPR036W4.983.26E-7VMA13Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase
YDL088C4.817.52E-7ASM4FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication
YNL100W4.779.13E-7AIM37Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
YOL043C4.681.46E-6NTG2DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication
YGR037C4.651.67E-6ACB1Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
YKL121W_p4.444.59E-6DGR2_pProtein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds
YDL075W4.279.77E-6RPL31ARibosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication
YOR124C4.231.17E-5UBP2Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity
YBL042C4.151.65E-5FUI1High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
YBR134W_d4.072.39E-5YBR134W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YCR025C_d3.914.57E-5YCR025C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene
YDR036C3.875.54E-5EHD33-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis
YIL071C3.797.48E-5PCI8Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain
YIL046W-A_p3.787.91E-5YIL046W-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YLR421C3.691.13E-4RPN13Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_1808
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0831.92E-10SGTC_750159-0059 199.0 μMChemDiv (Drug-like library)53772700.117647fatty acid desaturase (OLE1)
0.0823.34E-10SGTC_1896st058441 89.0 μMTimTec (Natural product derivative library)6887590.177419
0.0764.73E-9SGTC_31209124077 49.5 μMChembridge (Drug-like library)187743270.101266
0.0741.44E-8SGTC_33049125678 46.1 μMChembridge (Drug-like library)413162460.0945946
0.0722.74E-8SGTC_2525harmine 47.9 μMMiscellaneous52809530.0909091ergosterol biosynthesis
0.0699.40E-8SGTC_1819st053448 58.6 μMTimTec (Natural product derivative library)28576160.108108amide catabolism
0.0672.20E-7SGTC_2534helenine 20.1 μMMicrosource (Natural product library)727240.0684932RSC complex & mRNA processing
0.0663.21E-7SGTC_2129girgensonine 121.1 μMChembridge (Fragment library)4426380.0634921heme biosynthesis & mitochondrial translocase
0.0631.31E-6SGTC_8710574-0004 149.0 μMChemDiv (Drug-like library)95511730.0675676
0.0622.14E-6SGTC_1804st050076 49.1 μMTimTec (Natural product derivative library)38959760.6
0.0622.32E-6SGTC_13681852-0141 284.0 μMChemDiv (Drug-like library)28389870.0833333ERG2
0.0612.45E-6SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.0972222RSC & ERG11
0.0604.56E-6SGTC_13291447-1697 50.6 μMChemDiv (Drug-like library)28405150.155844
0.0595.60E-6SGTC_1969st069479 40.1 μMTimTec (Natural product derivative library)38954850.08
0.0571.00E-5SGTC_22016605497 169.9 μMChembridge (Fragment library)6707410.078125ergosterol biosynthesis

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1806st05000560.3 μM0.7727275194765TimTec (Natural product derivative library)331.836484.38313
SGTC_1800st05007929.07 μM0.739133645560TimTec (Natural product derivative library)373.487385.23713NEO1-PIK1
SGTC_1803st05006852.4 μM0.6938783984059TimTec (Natural product derivative library)381.388795.83917NEO1-PIK1
SGTC_1809st05008157.6 μM0.68755036894TimTec (Natural product derivative library)347.45014.62713
SGTC_1802st05011257.9 μM0.664662563TimTec (Natural product derivative library)345.4078633.90815
SGTC_1805st05001360.3 μM0.6530614037144TimTec (Natural product derivative library)331.836484.38313
SGTC_1760st04545310.45 μM0.6346153323419TimTec (Natural product derivative library)385.498085.38213NEO1-PIK1
SGTC_1807st05009258.9 μM0.6170215008496TimTec (Natural product derivative library)339.471165.1781360S ribosome export
SGTC_1801st05008928.44 μM0.6153855008493TimTec (Natural product derivative library)389.486785.27914NEO1-PIK1
SGTC_1811st05006961.5 μM0.6122453877702TimTec (Natural product derivative library)325.444584.69113