st050081

6,7-diethoxy-1-naphthalen-2-yl-1,2,3,4-tetrahydroisoquinoline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1809
Screen concentration 57.6 μM
Source TimTec (Natural product derivative library)
PubChem CID 5036894
SMILES CCOC1=C(C=C2C(NCCC2=C1)C3=CC4=CC=CC=C4C=C3)OCC
Standardized SMILES CCOc1cc2CCNC(c3ccc4ccccc4c3)c2cc1OCC
Molecular weight 347.4501
ALogP 4.63
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.48
% growth inhibition (Hom. pool) 7.13


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5036894
Download HIP data (tab-delimited text)  (excel)
Gene:BIG1(YHR101C)|FD-Score:4.49|P-value:3.60E-6|Clearance:0.08||SGD DESC:Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan Gene:ECM9(YKR004C)|FD-Score:-3.49|P-value:2.43E-4|Clearance:0||SGD DESC:Non-essential protein of unknown function Gene:ERG10(YPL028W)|FD-Score:3.21|P-value:6.69E-4|Clearance:0.16||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:ESP1(YGR098C)|FD-Score:-3.36|P-value:3.95E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:KAR1(YNL188W)|FD-Score:-3.88|P-value:5.13E-5|Clearance:0||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:NEO1(YIL048W)|FD-Score:8.89|P-value:3.12E-19|Clearance:3.92||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NUP49(YGL172W)|FD-Score:3.86|P-value:5.62E-5|Clearance:0.63||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PIK1(YNL267W)|FD-Score:4.4|P-value:5.33E-6|Clearance:0.09||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:POP6(YGR030C)|FD-Score:4.96|P-value:3.46E-7|Clearance:0.48||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RLP7(YNL002C)|FD-Score:3.24|P-value:6.05E-4|Clearance:0.03||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPN11(YFR004W)|FD-Score:4.31|P-value:7.99E-6|Clearance:0.45||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:SEC10(YLR166C)|FD-Score:-5.44|P-value:2.60E-8|Clearance:0||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:BIG1(YHR101C)|FD-Score:4.49|P-value:3.60E-6|Clearance:0.08||SGD DESC:Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan Gene:ECM9(YKR004C)|FD-Score:-3.49|P-value:2.43E-4|Clearance:0||SGD DESC:Non-essential protein of unknown function Gene:ERG10(YPL028W)|FD-Score:3.21|P-value:6.69E-4|Clearance:0.16||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:ESP1(YGR098C)|FD-Score:-3.36|P-value:3.95E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:KAR1(YNL188W)|FD-Score:-3.88|P-value:5.13E-5|Clearance:0||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:NEO1(YIL048W)|FD-Score:8.89|P-value:3.12E-19|Clearance:3.92||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NUP49(YGL172W)|FD-Score:3.86|P-value:5.62E-5|Clearance:0.63||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PIK1(YNL267W)|FD-Score:4.4|P-value:5.33E-6|Clearance:0.09||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:POP6(YGR030C)|FD-Score:4.96|P-value:3.46E-7|Clearance:0.48||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RLP7(YNL002C)|FD-Score:3.24|P-value:6.05E-4|Clearance:0.03||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPN11(YFR004W)|FD-Score:4.31|P-value:7.99E-6|Clearance:0.45||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:SEC10(YLR166C)|FD-Score:-5.44|P-value:2.60E-8|Clearance:0||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5036894
Download HOP data (tab-delimited text)  (excel)
Gene:ADE6(YGR061C)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ARF2(YDL137W)|FD-Score:-3.33|P-value:4.31E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:ARG82(YDR173C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARO9(YHR137W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:ATG7(YHR171W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:BER1(YLR412W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BNS1(YGR230W)|FD-Score:4.17|P-value:1.56E-5||SGD DESC:Protein with some similarity to Spo12p; overexpression bypasses need for Spo12p, but not required for meiosis Gene:BUD2(YKL092C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint Gene:CKB1(YGL019W)|FD-Score:8.87|P-value:3.64E-19||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CKB2(YOR039W)|FD-Score:4.38|P-value:5.91E-6||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CRS5(YOR031W)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:CUP9(YPL177C)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:ERV14(YGL054C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAU1(YER183C)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FCJ1(YKR016W)|FD-Score:5.39|P-value:3.48E-8||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:HST2(YPL015C)|FD-Score:6.35|P-value:1.09E-10||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HST3(YOR025W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:LIA1(YJR070C)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MNT3(YIL014W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MOT3(YMR070W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRPL10(YNL284C)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:MSS11(YMR164C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:NOT3(YIL038C)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NRM1(YNR009W)|FD-Score:5.13|P-value:1.45E-7||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:PDR1(YGL013C)|FD-Score:7.41|P-value:6.27E-14||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PET18(YCR020C)|FD-Score:5.92|P-value:1.61E-9||SGD DESC:Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin Gene:PHO88(YBR106W)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PPH21(YDL134C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication Gene:PRM2(YIL037C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:RAM1(YDL090C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:REC107(YJR021C)|FD-Score:-4.3|P-value:8.42E-6||SGD DESC:Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation Gene:RGL1(YPL066W)|FD-Score:5.05|P-value:2.22E-7||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RGP1(YDR137W)|FD-Score:-3.74|P-value:9.30E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RGS2(YOR107W)|FD-Score:3.77|P-value:8.21E-5||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RNH1(YMR234W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:RPL14A(YKL006W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SKI3(YPR189W)|FD-Score:3.86|P-value:5.71E-5||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SLS1(YLR139C)|FD-Score:-4.03|P-value:2.74E-5||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SLX8(YER116C)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SNF7(YLR025W)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPO7(YAL009W)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SYO1(YDL063C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TIR3(YIL011W)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TMA7(YLR262C-A)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TPM1(YNL079C)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TRM3(YDL112W)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TRS33(YOR115C)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UBP16(YPL072W)|FD-Score:-3.95|P-value:3.95E-5||SGD DESC:Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria Gene:VPS28(YPL065W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:5.36|P-value:4.05E-8||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:XDJ1(YLR090W)|FD-Score:4.29|P-value:9.05E-6||SGD DESC:Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YAP1801(YHR161C)|FD-Score:4.51|P-value:3.19E-6||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YBR196C-A(YBR196C-A_p)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR284W(YBR284W_p)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCR043C(YCR043C_p)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YDL119C(YDL119C_p)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR061W(YDR061W_p)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance Gene:YDR455C(YDR455C_d)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YER119C-A(YER119C-A_d)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGL007C-A(YGL007C-A_p)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGL118C(YGL118C_d)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL115C(YKL115C_d)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YLR257W(YLR257W_p)|FD-Score:5.67|P-value:7.11E-9||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YMR160W(YMR160W_p)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YOL164W-A(YOL164W-A_p)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YOR277C(YOR277C_d)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YPK1(YKL126W)|FD-Score:4.88|P-value:5.31E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ADE6(YGR061C)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ARF2(YDL137W)|FD-Score:-3.33|P-value:4.31E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:ARG82(YDR173C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARO9(YHR137W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:ATG7(YHR171W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:BER1(YLR412W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BNS1(YGR230W)|FD-Score:4.17|P-value:1.56E-5||SGD DESC:Protein with some similarity to Spo12p; overexpression bypasses need for Spo12p, but not required for meiosis Gene:BUD2(YKL092C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint Gene:CKB1(YGL019W)|FD-Score:8.87|P-value:3.64E-19||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CKB2(YOR039W)|FD-Score:4.38|P-value:5.91E-6||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CRS5(YOR031W)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:CUP9(YPL177C)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:ERV14(YGL054C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAU1(YER183C)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FCJ1(YKR016W)|FD-Score:5.39|P-value:3.48E-8||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:HST2(YPL015C)|FD-Score:6.35|P-value:1.09E-10||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HST3(YOR025W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:LIA1(YJR070C)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MNT3(YIL014W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MOT3(YMR070W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRPL10(YNL284C)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:MSS11(YMR164C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:NOT3(YIL038C)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NRM1(YNR009W)|FD-Score:5.13|P-value:1.45E-7||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:PDR1(YGL013C)|FD-Score:7.41|P-value:6.27E-14||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PET18(YCR020C)|FD-Score:5.92|P-value:1.61E-9||SGD DESC:Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin Gene:PHO88(YBR106W)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PPH21(YDL134C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication Gene:PRM2(YIL037C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:RAM1(YDL090C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:REC107(YJR021C)|FD-Score:-4.3|P-value:8.42E-6||SGD DESC:Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation Gene:RGL1(YPL066W)|FD-Score:5.05|P-value:2.22E-7||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RGP1(YDR137W)|FD-Score:-3.74|P-value:9.30E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RGS2(YOR107W)|FD-Score:3.77|P-value:8.21E-5||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RNH1(YMR234W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:RPL14A(YKL006W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SKI3(YPR189W)|FD-Score:3.86|P-value:5.71E-5||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SLS1(YLR139C)|FD-Score:-4.03|P-value:2.74E-5||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SLX8(YER116C)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SNF7(YLR025W)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPO7(YAL009W)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SYO1(YDL063C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TIR3(YIL011W)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TMA7(YLR262C-A)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TPM1(YNL079C)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TRM3(YDL112W)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TRS33(YOR115C)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UBP16(YPL072W)|FD-Score:-3.95|P-value:3.95E-5||SGD DESC:Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria Gene:VPS28(YPL065W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:5.36|P-value:4.05E-8||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:XDJ1(YLR090W)|FD-Score:4.29|P-value:9.05E-6||SGD DESC:Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YAP1801(YHR161C)|FD-Score:4.51|P-value:3.19E-6||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YBR196C-A(YBR196C-A_p)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR284W(YBR284W_p)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCR043C(YCR043C_p)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YDL119C(YDL119C_p)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR061W(YDR061W_p)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance Gene:YDR455C(YDR455C_d)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YER119C-A(YER119C-A_d)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGL007C-A(YGL007C-A_p)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGL118C(YGL118C_d)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL115C(YKL115C_d)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YLR257W(YLR257W_p)|FD-Score:5.67|P-value:7.11E-9||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YMR160W(YMR160W_p)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YOL164W-A(YOL164W-A_p)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YOR277C(YOR277C_d)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YPK1(YKL126W)|FD-Score:4.88|P-value:5.31E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL048W8.893.12E-193.92NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YGR030C4.963.46E-70.48POP6Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YHR101C4.493.60E-60.08BIG1Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan
YNL267W4.405.33E-60.09PIK1Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
YFR004W4.317.99E-60.45RPN11Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
YGL172W3.865.62E-50.63NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YNL002C3.246.05E-40.03RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
YPL028W3.216.69E-40.17ERG10Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis
YML126C3.040.001170.12ERG133-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
YPL082C2.930.001700.02MOT1Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA
YGR264C2.900.001840.06MES1Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs
YKL059C2.850.002220.01MPE1Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif
YBL004W2.830.002320.03UTP20Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
YOR282W_d2.800.002570.05YOR282W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
YLR163C2.750.002950.01MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL019W8.873.64E-19CKB1Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
YGL013C7.416.27E-14PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YPL015C6.351.09E-10HST2Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export
YCR020C5.921.61E-9PET18Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin
YLR257W_p5.677.11E-9YLR257W_pProtein of unknown function; protein abundance increases in response to DNA replication stress
YKR016W5.393.48E-8FCJ1Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane
YLR417W5.364.05E-8VPS36Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
YNR009W5.131.45E-7NRM1Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase
YPL066W5.052.22E-7RGL1Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
YKL126W4.885.31E-7YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YHR161C4.513.19E-6YAP1801Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication
YIL011W4.473.89E-6TIR3Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth
YOR039W4.385.91E-6CKB2Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
YLR090W4.299.05E-6XDJ1Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YGR230W4.171.56E-5BNS1Protein with some similarity to Spo12p; overexpression bypasses need for Spo12p, but not required for meiosis

GO enrichment analysis for SGTC_1809
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3023.91E-125SGTC_3133474-0040 5.0 μMChemDiv (Drug-like library)10433750.0724638NEO1-PIK1
0.2943.23E-118SGTC_11303454-2858 15.9 μMChemDiv (Drug-like library)7156280.0958904NEO1
0.2903.21E-115SGTC_3014119-0100 24.5 μMChemDiv (Drug-like library)28010.15942NEO1
0.2844.87E-110SGTC_1781st038725 66.3 μMTimTec (Natural product derivative library)42702750.368421NEO1
0.2832.60E-109SGTC_1721112-0046 2.6 μMChemDiv (Drug-like library)28021350.0821918NEO1
0.2837.15E-109SGTC_1760st045453 10.4 μMTimTec (Natural product derivative library)33234190.692308NEO1-PIK1
0.2815.58E-108SGTC_8880916-0002 7.0 μMChemDiv (Drug-like library)28021380.0821918NEO1
0.2793.46E-106SGTC_1991st073000 8.9 μMTimTec (Natural product derivative library)43019690.111111NEO1
0.2754.20E-103SGTC_270tamoxifen 5.5 μMMiscellaneous53760.149254NEO1
0.2721.65E-100SGTC_5352293-4129 48.2 μMChemDiv (Drug-like library)3961180.1NEO1
0.2693.12E-98SGTC_232clozapine 119.0 μMMiscellaneous28180.121622NEO1
0.2613.50E-92SGTC_22577960413 200.0 μMChembridge (Fragment library)29746890.15NEO1-PIK1
0.2606.21E-92SGTC_1797st048695 56.8 μMTimTec (Natural product derivative library)5922620.116883NEO1
0.2552.76E-88SGTC_1665st014639 11.4 μMTimTec (Natural product derivative library)9811330.0978261NEO1
0.2479.97E-83SGTC_9001112-0045 141.0 μMChemDiv (Drug-like library)562110.102941NEO1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1800st05007929.07 μM0.7291673645560TimTec (Natural product derivative library)373.487385.23713NEO1-PIK1
SGTC_1760st04545310.45 μM0.6923083323419TimTec (Natural product derivative library)385.498085.38213NEO1-PIK1
SGTC_1806st05000560.3 μM0.68755194765TimTec (Natural product derivative library)331.836484.38313
SGTC_1808st05006361.5 μM0.68754011993TimTec (Natural product derivative library)325.444584.66113
SGTC_1805st05001360.3 μM0.684037144TimTec (Natural product derivative library)331.836484.38313
SGTC_1801st05008928.44 μM0.6730775008493TimTec (Natural product derivative library)389.486785.27914NEO1-PIK1
SGTC_1804st05007649.1 μM0.6545453895976TimTec (Natural product derivative library)407.4772435.48515
SGTC_1802st05011257.9 μM0.6538464662563TimTec (Natural product derivative library)345.4078633.90815
SGTC_1803st05006852.4 μM0.6226423984059TimTec (Natural product derivative library)381.388795.83917NEO1-PIK1
SGTC_1810st05013853 μM0.5925933444541TimTec (Natural product derivative library)377.476084.61114PDR1