st050138

6,7-diethoxy-1-(4-methoxynaphthalen-1-yl)-1,2,3,4-tetrahydroisoquinoline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1810
Screen concentration 53.0 μM
Source TimTec (Natural product derivative library)
PubChem CID 3444541
SMILES CCOC1=C(C=C2C(NCCC2=C1)C3=CC=C(C4=CC=CC=C34)OC)OCC
Standardized SMILES CCOc1cc2CCNC(c2cc1OCC)c3ccc(OC)c4ccccc34
Molecular weight 377.4761
ALogP 4.61
H-bond donor count 1
H-bond acceptor count 4
Response signature PDR1

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.16
% growth inhibition (Hom. pool) 8.04


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3444541
Download HIP data (tab-delimited text)  (excel)
Gene:ATM1(YMR301C)|FD-Score:3.41|P-value:3.25E-4|Clearance:0.04||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:ERG8(YMR220W)|FD-Score:3.44|P-value:2.90E-4|Clearance:0.03||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:HRT1(YOL133W)|FD-Score:-3.12|P-value:8.91E-4|Clearance:0||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:MED7(YOL135C)|FD-Score:3.33|P-value:4.39E-4|Clearance:0.38||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MIA40(YKL195W)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.04||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NUG1(YER006W)|FD-Score:4.85|P-value:6.25E-7|Clearance:0.86||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:PRE8(YML092C)|FD-Score:-3.49|P-value:2.44E-4|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RAM2(YKL019W)|FD-Score:3.49|P-value:2.44E-4|Clearance:0.04||SGD DESC:Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor Gene:RPS3(YNL178W)|FD-Score:-3.26|P-value:5.58E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:SPC97(YHR172W)|FD-Score:-3.55|P-value:1.90E-4|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TAF7(YMR227C)|FD-Score:-3.41|P-value:3.23E-4|Clearance:0||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:TID3(YIL144W)|FD-Score:-3.77|P-value:8.01E-5|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:TRR1(YDR353W)|FD-Score:5|P-value:2.94E-7|Clearance:0.86||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:UTP6(YDR449C)|FD-Score:-3.11|P-value:9.42E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YBL077W(YBL077W_d)|FD-Score:3.87|P-value:5.34E-5|Clearance:0.39||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:YDR396W(YDR396W_d)|FD-Score:3.99|P-value:3.31E-5|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex Gene:YNL247W(YNL247W)|FD-Score:3.45|P-value:2.83E-4|Clearance:0.01||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments Gene:ATM1(YMR301C)|FD-Score:3.41|P-value:3.25E-4|Clearance:0.04||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:ERG8(YMR220W)|FD-Score:3.44|P-value:2.90E-4|Clearance:0.03||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:HRT1(YOL133W)|FD-Score:-3.12|P-value:8.91E-4|Clearance:0||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:MED7(YOL135C)|FD-Score:3.33|P-value:4.39E-4|Clearance:0.38||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MIA40(YKL195W)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.04||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NUG1(YER006W)|FD-Score:4.85|P-value:6.25E-7|Clearance:0.86||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:PRE8(YML092C)|FD-Score:-3.49|P-value:2.44E-4|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RAM2(YKL019W)|FD-Score:3.49|P-value:2.44E-4|Clearance:0.04||SGD DESC:Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor Gene:RPS3(YNL178W)|FD-Score:-3.26|P-value:5.58E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:SPC97(YHR172W)|FD-Score:-3.55|P-value:1.90E-4|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TAF7(YMR227C)|FD-Score:-3.41|P-value:3.23E-4|Clearance:0||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:TID3(YIL144W)|FD-Score:-3.77|P-value:8.01E-5|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:TRR1(YDR353W)|FD-Score:5|P-value:2.94E-7|Clearance:0.86||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:UTP6(YDR449C)|FD-Score:-3.11|P-value:9.42E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YBL077W(YBL077W_d)|FD-Score:3.87|P-value:5.34E-5|Clearance:0.39||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:YDR396W(YDR396W_d)|FD-Score:3.99|P-value:3.31E-5|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex Gene:YNL247W(YNL247W)|FD-Score:3.45|P-value:2.83E-4|Clearance:0.01||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3444541
Download HOP data (tab-delimited text)  (excel)
Gene:AIM18(YHR198C)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:BST1(YFL025C)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CAN1(YEL063C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CCE1(YKL011C)|FD-Score:3.77|P-value:8.14E-5||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:ERV14(YGL054C)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FMP27(YLR454W_p)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRA1(YLL029W)|FD-Score:-3.12|P-value:9.08E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:FYV12(YOR183W_p)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GYP1(YOR070C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:MGR1(YCL044C)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:NIT2(YJL126W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:OSH2(YDL019C)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PDR1(YGL013C)|FD-Score:8.76|P-value:9.93E-19||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEP7(YDR323C)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEP8(YJL053W)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:QDR1(YIL120W)|FD-Score:-5|P-value:2.87E-7||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SNF7(YLR025W)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPH1(YLR313C)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SPT21(YMR179W)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPT3(YDR392W)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:STF2(YGR008C)|FD-Score:4.27|P-value:9.85E-6||SGD DESC:Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress Gene:STP4(YDL048C)|FD-Score:4.42|P-value:4.88E-6||SGD DESC:Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor; relative distribution to the nucleus increases upon DNA replication stress Gene:VAM6(YDL077C)|FD-Score:3.72|P-value:9.82E-5||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VMA1(YDL185W)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS69(YPR087W_d)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:VPS70(YJR126C)|FD-Score:4.41|P-value:5.15E-6||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:VTC2(YFL004W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:YCL046W(YCL046W_d)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YDR455C(YDR455C_d)|FD-Score:3.72|P-value:9.97E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YGL176C(YGL176C_p)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YKR015C(YKR015C_p)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Putative protein of unknown function Gene:YLR307C-A(YLR307C-A_p)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Putative protein of unknown function Gene:YOL047C(YOL047C_p)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YOR114W(YOR114W_p)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YOR345C(YOR345C_d)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YOR376W-A(YOR376W-A_p)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:AIM18(YHR198C)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:BST1(YFL025C)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CAN1(YEL063C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CCE1(YKL011C)|FD-Score:3.77|P-value:8.14E-5||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:ERV14(YGL054C)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FMP27(YLR454W_p)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRA1(YLL029W)|FD-Score:-3.12|P-value:9.08E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:FYV12(YOR183W_p)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GYP1(YOR070C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:MGR1(YCL044C)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:NIT2(YJL126W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:OSH2(YDL019C)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PDR1(YGL013C)|FD-Score:8.76|P-value:9.93E-19||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEP7(YDR323C)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEP8(YJL053W)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:QDR1(YIL120W)|FD-Score:-5|P-value:2.87E-7||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SNF7(YLR025W)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPH1(YLR313C)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SPT21(YMR179W)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPT3(YDR392W)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:STF2(YGR008C)|FD-Score:4.27|P-value:9.85E-6||SGD DESC:Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress Gene:STP4(YDL048C)|FD-Score:4.42|P-value:4.88E-6||SGD DESC:Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor; relative distribution to the nucleus increases upon DNA replication stress Gene:VAM6(YDL077C)|FD-Score:3.72|P-value:9.82E-5||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VMA1(YDL185W)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS69(YPR087W_d)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:VPS70(YJR126C)|FD-Score:4.41|P-value:5.15E-6||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:VTC2(YFL004W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:YCL046W(YCL046W_d)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YDR455C(YDR455C_d)|FD-Score:3.72|P-value:9.97E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YGL176C(YGL176C_p)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YKR015C(YKR015C_p)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Putative protein of unknown function Gene:YLR307C-A(YLR307C-A_p)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Putative protein of unknown function Gene:YOL047C(YOL047C_p)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YOR114W(YOR114W_p)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YOR345C(YOR345C_d)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YOR376W-A(YOR376W-A_p)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR353W5.002.94E-70.86TRR1Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication
YER006W4.856.25E-70.86NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus
YDR396W_d3.993.31E-50.12YDR396W_dDubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex
YBL077W_d3.875.34E-50.39YBL077W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C
YKL019W3.492.44E-40.04RAM2Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor
YNL247W3.452.83E-40.01YNL247WCysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments
YMR220W3.442.90E-40.03ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YMR301C3.413.25E-40.04ATM1Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol
YKL195W3.373.80E-40.04MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YOL135C3.334.39E-40.38MED7Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YNL137C2.950.001610.10NAM9Mitochondrial ribosomal component of the small subunit
YGL091C2.850.002220.01NBP35Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases
YER043C2.840.002290.03SAH1S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor
YDR394W2.800.002530.05RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YNL287W2.750.002990.01SEC21Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL013C8.769.93E-19PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YDR392W4.671.52E-6SPT3Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
YFL025C4.542.85E-6BST1GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules
YDR323C4.523.11E-6PEP7Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
YDL048C4.424.88E-6STP4Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor; relative distribution to the nucleus increases upon DNA replication stress
YJR126C4.415.15E-6VPS70Protein of unknown function involved in vacuolar protein sorting
YGR008C4.279.85E-6STF2Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress
YOR114W_p4.131.81E-5YOR114W_pPutative protein of unknown function; null mutant is viable
YDR129C4.102.03E-5SAC6Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YJL053W4.082.24E-5PEP8Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
YDL019C3.924.42E-5OSH2Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
YHR198C3.914.68E-5AIM18Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
YOR376W-A_p3.895.00E-5YOR376W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YKL011C3.778.14E-5CCE1Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA
YDL077C3.729.82E-5VAM6Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p

GO enrichment analysis for SGTC_1810
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1996.56E-54SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.0779221PDR1
0.1804.33E-44SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.0615385PDR1
0.1778.93E-43SGTC_32419133421 49.5 μMChembridge (Drug-like library)170182210.076087PDR1
0.1751.01E-41SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.147727PDR1
0.1715.68E-40SGTC_8410251-0215 91.2 μMChemDiv (Drug-like library)38886140.05
0.1695.68E-39SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.0721649PDR1
0.1681.08E-38SGTC_6641222-0054 14.9 μMChemDiv (Drug-like library)68139910.0921053sphingolipid biosynthesis & PDR1
0.1602.57E-35SGTC_1687st020778 55.4 μMTimTec (Natural product derivative library)42592990.13253
0.1552.71E-33SGTC_12770873-0036 22.0 μMChemDiv (Drug-like library)59657350.0641026PDR1
0.1476.17E-30SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.0625PDR1
0.1427.30E-28SGTC_1694st024011 44.9 μMTimTec (Natural product derivative library)27875330.154762ERG2
0.1404.51E-27SGTC_5850kpi-0023 191.0 μMChemDiv (Drug-like library)646010.0857143
0.1381.54E-26SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.113924ERG2
0.1352.20E-25SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.0945946PDR1
0.1322.85E-24SGTC_3014119-0100 24.5 μMChemDiv (Drug-like library)28010.15493NEO1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1811st05006961.5 μM0.6153853877702TimTec (Natural product derivative library)325.444584.69113
SGTC_1800st05007929.07 μM0.6037743645560TimTec (Natural product derivative library)373.487385.23713NEO1-PIK1
SGTC_1802st05011257.9 μM0.64662563TimTec (Natural product derivative library)345.4078633.90815
SGTC_1809st05008157.6 μM0.5925935036894TimTec (Natural product derivative library)347.45014.62713
SGTC_1807st05009258.9 μM0.5882355008496TimTec (Natural product derivative library)339.471165.1781360S ribosome export
SGTC_1806st05000560.3 μM0.5660385194765TimTec (Natural product derivative library)331.836484.38313
SGTC_1808st05006361.5 μM0.5660384011993TimTec (Natural product derivative library)325.444584.66113
SGTC_1805st05001360.3 μM0.5636364037144TimTec (Natural product derivative library)331.836484.38313
SGTC_1801st05008928.44 μM0.5614045008493TimTec (Natural product derivative library)389.486785.27914NEO1-PIK1
SGTC_1760st04545310.45 μM0.5517243323419TimTec (Natural product derivative library)385.498085.38213NEO1-PIK1