st051246

(E)-1-(4-nitrophenyl)-3-phenylprop-2-en-1-one

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1813
Screen concentration 8.6 μM
Source TimTec (Natural product derivative library)
PubChem CID 5291959
SMILES C1=CC=C(C=C1)C=CC(=O)C2=CC=C(C=C2)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1ccc(cc1)C(=O)C=Cc2ccccc2
Molecular weight 253.2527
ALogP 3.6
H-bond donor count 0
H-bond acceptor count 3
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.87
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5291959
Download HIP data (tab-delimited text)  (excel)
Gene:ALG1(YBR110W)|FD-Score:3.18|P-value:7.48E-4|Clearance:0.28||SGD DESC:Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog Gene:CCT2(YIL142W)|FD-Score:-3.13|P-value:8.63E-4|Clearance:0||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT6(YDR188W)|FD-Score:-3.46|P-value:2.71E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:GPI10(YGL142C)|FD-Score:3.78|P-value:7.99E-5|Clearance:0.6||SGD DESC:Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog Gene:MIM1(YOL026C)|FD-Score:4.34|P-value:7.02E-6|Clearance:0.01||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:PAN1(YIR006C)|FD-Score:4.33|P-value:7.32E-6|Clearance:0.56||SGD DESC:Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease Gene:RPC53(YDL150W)|FD-Score:-3.44|P-value:2.88E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RPL17A(YKL180W)|FD-Score:-3.99|P-value:3.33E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SMD2(YLR275W)|FD-Score:-3.1|P-value:9.61E-4|Clearance:0||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:TRR1(YDR353W)|FD-Score:5.35|P-value:4.50E-8|Clearance:1||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:ALG1(YBR110W)|FD-Score:3.18|P-value:7.48E-4|Clearance:0.28||SGD DESC:Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog Gene:CCT2(YIL142W)|FD-Score:-3.13|P-value:8.63E-4|Clearance:0||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT6(YDR188W)|FD-Score:-3.46|P-value:2.71E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:GPI10(YGL142C)|FD-Score:3.78|P-value:7.99E-5|Clearance:0.6||SGD DESC:Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog Gene:MIM1(YOL026C)|FD-Score:4.34|P-value:7.02E-6|Clearance:0.01||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:PAN1(YIR006C)|FD-Score:4.33|P-value:7.32E-6|Clearance:0.56||SGD DESC:Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease Gene:RPC53(YDL150W)|FD-Score:-3.44|P-value:2.88E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RPL17A(YKL180W)|FD-Score:-3.99|P-value:3.33E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SMD2(YLR275W)|FD-Score:-3.1|P-value:9.61E-4|Clearance:0||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:TRR1(YDR353W)|FD-Score:5.35|P-value:4.50E-8|Clearance:1||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5291959
Download HOP data (tab-delimited text)  (excel)
Gene:ACO2(YJL200C)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol Gene:ALF1(YNL148C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ASE1(YOR058C)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:BEM4(YPL161C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:CAD1(YDR423C)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication Gene:CCS1(YMR038C)|FD-Score:4.42|P-value:4.95E-6||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:COG8(YML071C)|FD-Score:5.47|P-value:2.24E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:GAS1(YMR307W)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GUT1(YHL032C)|FD-Score:4.44|P-value:4.59E-6||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HAL1(YPR005C)|FD-Score:4.6|P-value:2.12E-6||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:ISR1(YPR106W)|FD-Score:-3.8|P-value:7.13E-5||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:KAP123(YER110C)|FD-Score:-3.35|P-value:4.03E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:MPM1(YJL066C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:NTG2(YOL043C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication Gene:PLB2(YMR006C)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:RAD51(YER095W)|FD-Score:5|P-value:2.87E-7||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RPL20B(YOR312C)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPS23B(YPR132W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RPS29A(YLR388W)|FD-Score:-5.5|P-value:1.92E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:-4.03|P-value:2.85E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:SDH1(YKL148C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SIP4(YJL089W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SLX9(YGR081C)|FD-Score:-4.07|P-value:2.34E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SOD1(YJR104C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPO1(YNL012W)|FD-Score:5.91|P-value:1.67E-9||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:SPT4(YGR063C)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:TIF4631(YGR162W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:5.59|P-value:1.11E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSC3(YBR058C-A)|FD-Score:21.4|P-value:4.28E-102||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMA5(YKL080W)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YAL016C-B(YAL016C-B_d)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR182W-A(YDR182W-A_p)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YEL028W(YEL028W_d)|FD-Score:-3.98|P-value:3.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL041C-B(YGL041C-B_p)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YKR015C(YKR015C_p)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Putative protein of unknown function Gene:YMR194C-A(YMR194C-A_d)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL155W(YNL155W_p)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YPL073C(YPL073C_d)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPL114W(YPL114W_d)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPR170C(YPR170C_d)|FD-Score:3.9|P-value:4.74E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:ACO2(YJL200C)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol Gene:ALF1(YNL148C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ASE1(YOR058C)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:BEM4(YPL161C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:CAD1(YDR423C)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication Gene:CCS1(YMR038C)|FD-Score:4.42|P-value:4.95E-6||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:COG8(YML071C)|FD-Score:5.47|P-value:2.24E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:GAS1(YMR307W)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GUT1(YHL032C)|FD-Score:4.44|P-value:4.59E-6||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HAL1(YPR005C)|FD-Score:4.6|P-value:2.12E-6||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:ISR1(YPR106W)|FD-Score:-3.8|P-value:7.13E-5||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:KAP123(YER110C)|FD-Score:-3.35|P-value:4.03E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:MPM1(YJL066C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:NTG2(YOL043C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication Gene:PLB2(YMR006C)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:RAD51(YER095W)|FD-Score:5|P-value:2.87E-7||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RPL20B(YOR312C)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPS23B(YPR132W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RPS29A(YLR388W)|FD-Score:-5.5|P-value:1.92E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:-4.03|P-value:2.85E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:SDH1(YKL148C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SIP4(YJL089W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SLX9(YGR081C)|FD-Score:-4.07|P-value:2.34E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SOD1(YJR104C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPO1(YNL012W)|FD-Score:5.91|P-value:1.67E-9||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:SPT4(YGR063C)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:TIF4631(YGR162W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:5.59|P-value:1.11E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSC3(YBR058C-A)|FD-Score:21.4|P-value:4.28E-102||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMA5(YKL080W)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YAL016C-B(YAL016C-B_d)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR182W-A(YDR182W-A_p)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YEL028W(YEL028W_d)|FD-Score:-3.98|P-value:3.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL041C-B(YGL041C-B_p)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YKR015C(YKR015C_p)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Putative protein of unknown function Gene:YMR194C-A(YMR194C-A_d)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL155W(YNL155W_p)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YPL073C(YPL073C_d)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPL114W(YPL114W_d)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPR170C(YPR170C_d)|FD-Score:3.9|P-value:4.74E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR353W5.354.50E-81.00TRR1Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication
YOL026C4.347.02E-60.01MIM1Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex
YIR006C4.337.32E-60.56PAN1Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease
YGL142C3.787.99E-50.60GPI10Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog
YBR110W3.187.48E-40.28ALG1Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog
YPR175W2.900.001870.01DPB2Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate
YHR101C2.890.001930.04BIG1Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan
YLL036C2.850.002170.06PRP19Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
YER043C2.800.002580.05SAH1S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor
YOL034W2.750.002970.05SMC5Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair
YLR196W2.700.003440.15PWP1Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily
YJL109C2.550.005380.02UTP10Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance
YBR089W_d2.530.005630.01YBR089W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30
YBR243C2.530.005770.04ALG7UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
YMR277W2.490.006410.03FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A21.404.28E-102TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YNL012W5.911.67E-9SPO1Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B
YER090W5.591.11E-8TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YML071C5.472.24E-8COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YER095W5.002.87E-7RAD51Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
YPR005C4.602.12E-6HAL1Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p
YHL032C4.444.59E-6GUT1Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p
YMR038C4.424.95E-6CCS1Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress
YMR194C-A_d4.221.20E-5YMR194C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YJL089W4.171.54E-5SIP4C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus
YKR015C_p4.161.62E-5YKR015C_pPutative protein of unknown function
YPR170C_d3.904.74E-5YPR170C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B
YMR006C3.895.00E-5PLB2Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine
YPL161C3.711.05E-4BEM4Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length
YJR104C3.621.45E-4SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid

GO enrichment analysis for SGTC_1813
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.5220SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.128571TSC3-RPN4
0.5030SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0588235TSC3-RPN4
0.5000SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0333333TSC3-RPN4
0.4810SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.333333TSC3-RPN4
0.4609.61E-308SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.306122TSC3-RPN4
0.4591.20E-305SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.206897TSC3-RPN4
0.4572.81E-302SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.34TSC3-RPN4
0.4276.56E-260SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.153846TSC3-RPN4
0.4219.58E-253SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.0789474TSC3-RPN4
0.4166.80E-246SGTC_1815st051655 58.2 μMTimTec (Natural product derivative library)59841560.0972222TSC3-RPN4
0.4034.74E-229SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.326531TSC3-RPN4
0.3996.97E-225SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.05TSC3-RPN4
0.3989.34E-224SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.147541TSC3-RPN4
0.3961.98E-220SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0508475TSC3-RPN4
0.3894.29E-212SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.188679

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_26044'-hydroxychalcone11.32 μM0.6111115282362Microsource (Natural product library)224.254583.4612
SGTC_8360330-01041.35 μM0.56410346494820ChemDiv (Drug-like library)294.518543.6103
SGTC_19284'-methoxychalcone40.3 μM0.564103641818TimTec (Natural product derivative library)238.281163.68502
SGTC_24984'-Methoxychalcone54.63 μM0.564103641818Microsource (Natural product library)238.281163.68502RPP1 & pyrimidine depletion
SGTC_1608st00147340.5 μM0.5263167292267TimTec (Natural product derivative library)242.700244.36601
SGTC_1967st07017751.4 μM0.5185195729214TimTec (Natural product derivative library)389.400665.14705
SGTC_1828st05581149 μM0.4905665877589TimTec (Natural product derivative library)407.803265.69205
SGTC_9491165-0505146 μM0.4772736244267ChemDiv (Drug-like library)304.321082.15415
SGTC_5671080-054268.4 μM0.43755950415ChemDiv (Drug-like library)296.277483.3115
SGTC_7991310-0557151 μM0.4130435354492ChemDiv (Drug-like library)252.264683.4703superoxide