st052110

5-bromo-1-[2-(4-chlorophenoxy)ethyl]pyrimidine-2,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1814
Screen concentration 31.5 μM
Source TimTec (Natural product derivative library)
PubChem CID 674163
SMILES C1=CC(=CC=C1OCCN2C=C(C(=O)NC2=O)Br)Cl
Standardized SMILES Clc1ccc(OCCN2C=C(Br)C(=O)NC2=O)cc1
Molecular weight 345.5764
ALogP 1.94
H-bond donor count 1
H-bond acceptor count 3
Response signature RNA processing & uracil transport

Pool Growth Kinetics
% growth inhibition (Het. pool) 20.91
% growth inhibition (Hom. pool) 6.36


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 674163
Download HIP data (tab-delimited text)  (excel)
Gene:AFG2(YLR397C)|FD-Score:3.4|P-value:3.41E-4|Clearance:0.01||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:CDC24(YAL041W)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.02||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CDC4(YFL009W)|FD-Score:3.21|P-value:6.59E-4|Clearance:0.06||SGD DESC:F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p Gene:ECM9(YKR004C)|FD-Score:-3.43|P-value:3.05E-4|Clearance:0||SGD DESC:Non-essential protein of unknown function Gene:IRA1(YBR140C)|FD-Score:8.28|P-value:5.91E-17|Clearance:2.02||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MES1(YGR264C)|FD-Score:3.85|P-value:5.96E-5|Clearance:0.1||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:NOC2(YOR206W)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.15||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOP8(YOL144W)|FD-Score:-3.78|P-value:7.84E-5|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PCF11(YDR228C)|FD-Score:3.24|P-value:5.89E-4|Clearance:0.01||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:POP6(YGR030C)|FD-Score:3.38|P-value:3.67E-4|Clearance:0.04||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE10(YOR362C)|FD-Score:3.49|P-value:2.37E-4|Clearance:0.03||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE5(YMR314W)|FD-Score:4.07|P-value:2.33E-5|Clearance:0.22||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE6(YOL038W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.05||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRE8(YML092C)|FD-Score:3.47|P-value:2.64E-4|Clearance:0.07||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PUP3(YER094C)|FD-Score:4.49|P-value:3.56E-6|Clearance:0.16||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RPC53(YDL150W)|FD-Score:6.27|P-value:1.85E-10|Clearance:0.92||SGD DESC:RNA polymerase III subunit C53 Gene:RPL15A(YLR029C)|FD-Score:11.1|P-value:7.95E-29|Clearance:2.79||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RRP45(YDR280W)|FD-Score:4.33|P-value:7.39E-6|Clearance:0.26||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RSM10(YDR041W)|FD-Score:3.39|P-value:3.52E-4|Clearance:0.01||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins Gene:SEC65(YML105C)|FD-Score:3.75|P-value:8.92E-5|Clearance:0.05||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:TRM5(YHR070W)|FD-Score:3.15|P-value:8.11E-4|Clearance:0.23||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:TRS120(YDR407C)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.1||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:YDL152W(YDL152W_d)|FD-Score:5.35|P-value:4.46E-8|Clearance:0.8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YDR327W(YDR327W_d)|FD-Score:4.55|P-value:2.74E-6|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1 Gene:YGR114C(YGR114C_d)|FD-Score:-4.16|P-value:1.57E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YJL086C(YJL086C_d)|FD-Score:-3.38|P-value:3.66E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:AFG2(YLR397C)|FD-Score:3.4|P-value:3.41E-4|Clearance:0.01||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:CDC24(YAL041W)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.02||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CDC4(YFL009W)|FD-Score:3.21|P-value:6.59E-4|Clearance:0.06||SGD DESC:F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p Gene:ECM9(YKR004C)|FD-Score:-3.43|P-value:3.05E-4|Clearance:0||SGD DESC:Non-essential protein of unknown function Gene:IRA1(YBR140C)|FD-Score:8.28|P-value:5.91E-17|Clearance:2.02||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MES1(YGR264C)|FD-Score:3.85|P-value:5.96E-5|Clearance:0.1||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:NOC2(YOR206W)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.15||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOP8(YOL144W)|FD-Score:-3.78|P-value:7.84E-5|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PCF11(YDR228C)|FD-Score:3.24|P-value:5.89E-4|Clearance:0.01||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:POP6(YGR030C)|FD-Score:3.38|P-value:3.67E-4|Clearance:0.04||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE10(YOR362C)|FD-Score:3.49|P-value:2.37E-4|Clearance:0.03||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE5(YMR314W)|FD-Score:4.07|P-value:2.33E-5|Clearance:0.22||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE6(YOL038W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.05||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRE8(YML092C)|FD-Score:3.47|P-value:2.64E-4|Clearance:0.07||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PUP3(YER094C)|FD-Score:4.49|P-value:3.56E-6|Clearance:0.16||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RPC53(YDL150W)|FD-Score:6.27|P-value:1.85E-10|Clearance:0.92||SGD DESC:RNA polymerase III subunit C53 Gene:RPL15A(YLR029C)|FD-Score:11.1|P-value:7.95E-29|Clearance:2.79||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RRP45(YDR280W)|FD-Score:4.33|P-value:7.39E-6|Clearance:0.26||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RSM10(YDR041W)|FD-Score:3.39|P-value:3.52E-4|Clearance:0.01||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins Gene:SEC65(YML105C)|FD-Score:3.75|P-value:8.92E-5|Clearance:0.05||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:TRM5(YHR070W)|FD-Score:3.15|P-value:8.11E-4|Clearance:0.23||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:TRS120(YDR407C)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.1||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:YDL152W(YDL152W_d)|FD-Score:5.35|P-value:4.46E-8|Clearance:0.8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YDR327W(YDR327W_d)|FD-Score:4.55|P-value:2.74E-6|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1 Gene:YGR114C(YGR114C_d)|FD-Score:-4.16|P-value:1.57E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YJL086C(YJL086C_d)|FD-Score:-3.38|P-value:3.66E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 674163
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:6.21|P-value:2.68E-10||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AEP3(YPL005W)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AIM18(YHR198C)|FD-Score:4.54|P-value:2.75E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ANP1(YEL036C)|FD-Score:-3.79|P-value:7.60E-5||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:ATG27(YJL178C)|FD-Score:5.68|P-value:6.88E-9||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BIO3(YNR058W)|FD-Score:4.99|P-value:3.03E-7||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BMH1(YER177W)|FD-Score:-4.14|P-value:1.74E-5||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BPH1(YCR032W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BUD16(YEL029C)|FD-Score:-4.82|P-value:7.34E-7||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CDC55(YGL190C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CDC73(YLR418C)|FD-Score:5.08|P-value:1.84E-7||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLC1(YGR167W)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COX6(YHR051W)|FD-Score:-3.13|P-value:8.67E-4||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CTR9(YOL145C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DUF1(YOL087C)|FD-Score:3.74|P-value:9.20E-5||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ECM34(YHL043W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:ELA1(YNL230C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:FUR4(YBR021W)|FD-Score:18|P-value:1.18E-72||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GDT1(YBR187W_p)|FD-Score:4.9|P-value:4.89E-7||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GLO1(YML004C)|FD-Score:6.58|P-value:2.40E-11||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GLY1(YEL046C)|FD-Score:-5.48|P-value:2.16E-8||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:ITC1(YGL133W)|FD-Score:-5.43|P-value:2.86E-8||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:MBB1(YJL199C_d)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Gene:MGR2(YPL098C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MHR1(YDR296W)|FD-Score:-3.09|P-value:9.96E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MRP20(YDR405W)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSD1(YPL104W)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MSS116(YDR194C)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:MTC4(YBR255W)|FD-Score:-4.07|P-value:2.32E-5||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:MTQ2(YDR140W)|FD-Score:-4.35|P-value:6.95E-6||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:MVB12(YGR206W)|FD-Score:-3.72|P-value:9.83E-5||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NVJ1(YHR195W)|FD-Score:3.81|P-value:6.83E-5||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:ORM1(YGR038W)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PDR16(YNL231C)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PIF1(YML061C)|FD-Score:-4.1|P-value:2.06E-5||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PRM1(YNL279W)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PUS1(YPL212C)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:RFM1(YOR279C)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPN4(YDL020C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SDC1(YDR469W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SIP5(YMR140W)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SLX9(YGR081C)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SSN3(YPL042C)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SSQ1(YLR369W)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:TAN1(YGL232W)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TDA8(YAL064C-A_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:VPS28(YPL065W)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:-3.1|P-value:9.77E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS69(YPR087W_d)|FD-Score:-3.13|P-value:8.78E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YBR196C-A(YBR196C-A_p)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR161W(YDR161W_p)|FD-Score:-3.2|P-value:6.77E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YGL177W(YGL177W_d)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL068W-A(YKL068W-A_p)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR264C-A(YLR264C-A_p)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Putative protein of unknown function Gene:YMR084W(YMR084W_p)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YOR097C(YOR097C_p)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:ACB1(YGR037C)|FD-Score:6.21|P-value:2.68E-10||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AEP3(YPL005W)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AIM18(YHR198C)|FD-Score:4.54|P-value:2.75E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ANP1(YEL036C)|FD-Score:-3.79|P-value:7.60E-5||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:ATG27(YJL178C)|FD-Score:5.68|P-value:6.88E-9||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BIO3(YNR058W)|FD-Score:4.99|P-value:3.03E-7||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BMH1(YER177W)|FD-Score:-4.14|P-value:1.74E-5||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BPH1(YCR032W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BUD16(YEL029C)|FD-Score:-4.82|P-value:7.34E-7||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CDC55(YGL190C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CDC73(YLR418C)|FD-Score:5.08|P-value:1.84E-7||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLC1(YGR167W)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COX6(YHR051W)|FD-Score:-3.13|P-value:8.67E-4||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CTR9(YOL145C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DUF1(YOL087C)|FD-Score:3.74|P-value:9.20E-5||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ECM34(YHL043W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:ELA1(YNL230C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:FUR4(YBR021W)|FD-Score:18|P-value:1.18E-72||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GDT1(YBR187W_p)|FD-Score:4.9|P-value:4.89E-7||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GLO1(YML004C)|FD-Score:6.58|P-value:2.40E-11||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GLY1(YEL046C)|FD-Score:-5.48|P-value:2.16E-8||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:ITC1(YGL133W)|FD-Score:-5.43|P-value:2.86E-8||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:MBB1(YJL199C_d)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Gene:MGR2(YPL098C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MHR1(YDR296W)|FD-Score:-3.09|P-value:9.96E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MRP20(YDR405W)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSD1(YPL104W)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MSS116(YDR194C)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:MTC4(YBR255W)|FD-Score:-4.07|P-value:2.32E-5||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:MTQ2(YDR140W)|FD-Score:-4.35|P-value:6.95E-6||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:MVB12(YGR206W)|FD-Score:-3.72|P-value:9.83E-5||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NVJ1(YHR195W)|FD-Score:3.81|P-value:6.83E-5||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:ORM1(YGR038W)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PDR16(YNL231C)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PIF1(YML061C)|FD-Score:-4.1|P-value:2.06E-5||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PRM1(YNL279W)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PUS1(YPL212C)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:RFM1(YOR279C)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPN4(YDL020C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SDC1(YDR469W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SIP5(YMR140W)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SLX9(YGR081C)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SSN3(YPL042C)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SSQ1(YLR369W)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:TAN1(YGL232W)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TDA8(YAL064C-A_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:VPS28(YPL065W)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:-3.1|P-value:9.77E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS69(YPR087W_d)|FD-Score:-3.13|P-value:8.78E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YBR196C-A(YBR196C-A_p)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR161W(YDR161W_p)|FD-Score:-3.2|P-value:6.77E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YGL177W(YGL177W_d)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL068W-A(YKL068W-A_p)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR264C-A(YLR264C-A_p)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Putative protein of unknown function Gene:YMR084W(YMR084W_p)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YOR097C(YOR097C_p)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YLR029C11.107.95E-292.79RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YBR140C8.285.91E-172.02IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YDL150W6.271.85E-100.92RPC53RNA polymerase III subunit C53
YDL152W_d5.354.46E-80.80YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YDR327W_d4.552.74E-60.05YDR327W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1
YER094C4.493.56E-60.16PUP3Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
YDR280W4.337.39E-60.26RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YMR314W4.072.33E-50.22PRE5Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YGR264C3.855.96E-50.10MES1Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs
YML105C3.758.92E-50.05SEC65Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
YOL038W3.701.09E-40.05PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
YOR206W3.651.32E-40.15NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YOR362C3.492.37E-40.03PRE10Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YML092C3.472.64E-40.07PRE8Alpha 2 subunit of the 20S proteasome
YLR397C3.403.41E-40.01AFG2ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs

Top 15 HOM fitness defect scores (HOP)

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Deletion Strain FD score P-value Gene Gene Description
YBR021W18.001.18E-72FUR4Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
YML004C6.582.40E-11GLO1Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress
YGR037C6.212.68E-10ACB1Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
YJL178C5.686.88E-9ATG27Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site
YLR418C5.081.84E-7CDC73Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress
YNR058W4.993.03E-7BIO37,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis
YBR187W_p4.904.89E-7GDT1_pPutative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis
YHR198C4.542.75E-6AIM18Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
YOR279C4.161.56E-5RFM1DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance
YBR196C-A_p4.072.38E-5YBR196C-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YGL232W3.846.09E-5TAN1Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress
YHR195W3.816.83E-5NVJ1Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN)
YOL087C3.749.20E-5DUF1Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid
YGL177W_d3.701.07E-4YGL177W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOL145C3.671.22E-4CTR9Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats

GO enrichment analysis for SGTC_1814
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3132.25E-134SGTC_23939031399 185.9 μMChembridge (Fragment library)15266230.169492RNA processing & uracil transport
0.3095.64E-131SGTC_23519026858 72.3 μMChembridge (Fragment library)14093000.189655RNA processing & uracil transport
0.2857.71E-111SGTC_1471k673-0312 37.0 μMChemDiv (Drug-like library)240998840.0945946RNA processing & uracil transport
0.2554.04E-88SGTC_20825219693 150.0 μMChembridge (Fragment library)28346490.111111RNA processing & uracil transport
0.2415.15E-79SGTC_22977971671 152.3 μMChembridge (Fragment library)9090160.209677RNA processing & uracil transport
0.2264.08E-69SGTC_1817st052116 64.4 μMTimTec (Natural product derivative library)43613980.4RNA processing & uracil transport
0.1951.74E-51SGTC_8093998-0021 90.0 μMChemDiv (Drug-like library)7154850.0857143RNA processing & uracil transport
0.1898.53E-49SGTC_9231900-2434 73.3 μMChemDiv (Drug-like library)59105700.0506329RNA processing & uracil transport
0.1802.00E-44SGTC_33559151058 44.0 μMChembridge (Drug-like library)170157280.0526316RNA processing & uracil transport
0.1672.99E-38SGTC_1220236-0016 402.4 μMChemDiv (Drug-like library)68088380.0588235RNA processing & uracil transport
0.1623.54E-36SGTC_530973-0023 40.0 μMChemDiv (Drug-like library)6747680.0895522RNA processing & uracil transport
0.1555.31E-33SGTC_1950st077106 7.2 μMTimTec (Natural product derivative library)138318640.0681818RNA processing & uracil transport
0.1555.64E-33SGTC_1167k781-0610 148.0 μMChemDiv (Drug-like library)45251690.0704225RNA processing & uracil transport
0.1482.52E-30SGTC_452diindolylmethane 8.0 μMICCB bioactive library30710.0740741RNA processing & uracil transport
0.1403.30E-27SGTC_22927671323 168.1 μMChembridge (Fragment library)9375040.121212RNA processing & uracil transport

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1817st05211664.4 μM0.44361398TimTec (Natural product derivative library)310.73291.85514RNA processing & uracil transport
SGTC_2232chlorophetanol41.54 μM0.38095215907Miscellaneous172.608861.93812TRP & mitochondrial translation
SGTC_6033794-002345.9 μM0.3454553802260ChemDiv (Drug-like library)356.759823.09624
SGTC_7950685-026578 μM0.320755302754ChemDiv (Drug-like library)340.599643.86212excess fatty acid
SGTC_7864544-0069423 μM0.277778748821ChemDiv (Drug-like library)268.310442.73602
SGTC_2422chlorphenesin carbamate384.24 μM0.2777782724Miscellaneous245.659581.59724
SGTC_1300136-0219337.66 μM0.272727234244ChemDiv (Drug-like library)296.148643.77812
SGTC_1994063-002441.6 μM0.272727306215ChemDiv (Drug-like library)311.783863.31414
SGTC_2826799254119.48 μM0.2678572985066Chembridge (Drug-like library)297.6845063.52514
SGTC_3127912694649.47 μM0.26415127243249Chembridge (Drug-like library)223.698663.0212ERG2