st051655

6-[(2Z)-2-(4-chlorophenyl)-2-hydroxyiminoethoxy]-4-methylchromen-2-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1815
Screen concentration 58.2 μM
Source TimTec (Natural product derivative library)
PubChem CID 5984156
SMILES CC1=CC(=O)OC2=C1C=C(C=C2)OCC(=NO)C3=CC=C(C=C3)Cl
Standardized SMILES CC1=CC(=O)Oc2ccc(OCC(=NO)c3ccc(Cl)cc3)cc12
Molecular weight 343.7611
ALogP 4.32
H-bond donor count 1
H-bond acceptor count 5
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 56.22
% growth inhibition (Hom. pool) 5.37


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5984156
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:5.28|P-value:6.32E-8|Clearance:0.6||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:DAD2(YKR083C)|FD-Score:-3.41|P-value:3.28E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ERG1(YGR175C)|FD-Score:4.38|P-value:5.92E-6|Clearance:0.08||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:ERG11(YHR007C)|FD-Score:4.01|P-value:3.10E-5|Clearance:0.01||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GCD11(YER025W)|FD-Score:3.42|P-value:3.16E-4|Clearance:0.02||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:HCA4(YJL033W)|FD-Score:7.97|P-value:8.02E-16|Clearance:2||SGD DESC:DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis Gene:MCM10(YIL150C)|FD-Score:-3.96|P-value:3.81E-5|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:NOP58(YOR310C)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.11||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PRP19(YLL036C)|FD-Score:3.17|P-value:7.64E-4|Clearance:0.02||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:PRP6(YBR055C)|FD-Score:3.14|P-value:8.55E-4|Clearance:0.11||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PUP1(YOR157C)|FD-Score:3.75|P-value:8.75E-5|Clearance:0.14||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:QNS1(YHR074W)|FD-Score:4.68|P-value:1.44E-6|Clearance:0.08||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RAM2(YKL019W)|FD-Score:3.4|P-value:3.36E-4|Clearance:0.08||SGD DESC:Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor Gene:RIM2(YBR192W)|FD-Score:10.3|P-value:2.26E-25|Clearance:2.37||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPB3(YIL021W)|FD-Score:4.52|P-value:3.04E-6|Clearance:0.02||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB7(YDR404C)|FD-Score:4.5|P-value:3.42E-6|Clearance:0.12||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPL5(YPL131W)|FD-Score:3.45|P-value:2.77E-4|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPP1(YHR062C)|FD-Score:4.3|P-value:8.40E-6|Clearance:0.19||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS15(YOL040C)|FD-Score:3.15|P-value:8.10E-4|Clearance:0.02||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS2(YGL123W)|FD-Score:3.92|P-value:4.52E-5|Clearance:0.05||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:SEN15(YMR059W)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.16||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SPC110(YDR356W)|FD-Score:-3.14|P-value:8.34E-4|Clearance:0||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:TFC1(YBR123C)|FD-Score:5.97|P-value:1.18E-9|Clearance:0.03||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TFC3(YAL001C)|FD-Score:5.94|P-value:1.41E-9|Clearance:0.63||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TOM22(YNL131W)|FD-Score:4.6|P-value:2.15E-6|Clearance:0.07||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TRR1(YDR353W)|FD-Score:3.32|P-value:4.55E-4|Clearance:0.04||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:UTR5(YEL035C_p)|FD-Score:3.28|P-value:5.25E-4|Clearance:0.11||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YDL016C(YDL016C_d)|FD-Score:3.86|P-value:5.61E-5|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YJL195C(YJL195C_d)|FD-Score:3.99|P-value:3.24E-5|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YLR458W(YLR458W_d)|FD-Score:5.31|P-value:5.51E-8|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis Gene:YTH1(YPR107C)|FD-Score:-3.11|P-value:9.31E-4|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ARP7(YPR034W)|FD-Score:5.28|P-value:6.32E-8|Clearance:0.6||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:DAD2(YKR083C)|FD-Score:-3.41|P-value:3.28E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ERG1(YGR175C)|FD-Score:4.38|P-value:5.92E-6|Clearance:0.08||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:ERG11(YHR007C)|FD-Score:4.01|P-value:3.10E-5|Clearance:0.01||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GCD11(YER025W)|FD-Score:3.42|P-value:3.16E-4|Clearance:0.02||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:HCA4(YJL033W)|FD-Score:7.97|P-value:8.02E-16|Clearance:2||SGD DESC:DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis Gene:MCM10(YIL150C)|FD-Score:-3.96|P-value:3.81E-5|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:NOP58(YOR310C)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.11||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PRP19(YLL036C)|FD-Score:3.17|P-value:7.64E-4|Clearance:0.02||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:PRP6(YBR055C)|FD-Score:3.14|P-value:8.55E-4|Clearance:0.11||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PUP1(YOR157C)|FD-Score:3.75|P-value:8.75E-5|Clearance:0.14||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:QNS1(YHR074W)|FD-Score:4.68|P-value:1.44E-6|Clearance:0.08||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RAM2(YKL019W)|FD-Score:3.4|P-value:3.36E-4|Clearance:0.08||SGD DESC:Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor Gene:RIM2(YBR192W)|FD-Score:10.3|P-value:2.26E-25|Clearance:2.37||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPB3(YIL021W)|FD-Score:4.52|P-value:3.04E-6|Clearance:0.02||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB7(YDR404C)|FD-Score:4.5|P-value:3.42E-6|Clearance:0.12||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPL5(YPL131W)|FD-Score:3.45|P-value:2.77E-4|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPP1(YHR062C)|FD-Score:4.3|P-value:8.40E-6|Clearance:0.19||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS15(YOL040C)|FD-Score:3.15|P-value:8.10E-4|Clearance:0.02||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS2(YGL123W)|FD-Score:3.92|P-value:4.52E-5|Clearance:0.05||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:SEN15(YMR059W)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.16||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SPC110(YDR356W)|FD-Score:-3.14|P-value:8.34E-4|Clearance:0||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:TFC1(YBR123C)|FD-Score:5.97|P-value:1.18E-9|Clearance:0.03||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TFC3(YAL001C)|FD-Score:5.94|P-value:1.41E-9|Clearance:0.63||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TOM22(YNL131W)|FD-Score:4.6|P-value:2.15E-6|Clearance:0.07||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TRR1(YDR353W)|FD-Score:3.32|P-value:4.55E-4|Clearance:0.04||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:UTR5(YEL035C_p)|FD-Score:3.28|P-value:5.25E-4|Clearance:0.11||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YDL016C(YDL016C_d)|FD-Score:3.86|P-value:5.61E-5|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YJL195C(YJL195C_d)|FD-Score:3.99|P-value:3.24E-5|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YLR458W(YLR458W_d)|FD-Score:5.31|P-value:5.51E-8|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis Gene:YTH1(YPR107C)|FD-Score:-3.11|P-value:9.31E-4|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5984156
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ARL1(YBR164C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ATG19(YOL082W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles Gene:BCH1(YMR237W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:CBS1(YDL069C)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CDC26(YFR036W)|FD-Score:3.82|P-value:6.67E-5||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CLB2(YPR119W)|FD-Score:4.39|P-value:5.79E-6||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CLB3(YDL155W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:COG8(YML071C)|FD-Score:7.65|P-value:1.04E-14||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUP9(YPL177C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:DCC1(YCL016C)|FD-Score:-4.57|P-value:2.44E-6||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:FAR11(YNL127W)|FD-Score:4.98|P-value:3.20E-7||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FEN1(YCR034W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:GCN3(YKR026C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:HAL1(YPR005C)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:HER2(YMR293C)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:IRC21(YMR073C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:JJJ1(YNL227C)|FD-Score:-3.1|P-value:9.73E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:KRE28(YDR532C)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:LRO1(YNR008W)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MCM16(YPR046W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MDG1(YNL173C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MHR1(YDR296W)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MLS1(YNL117W)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MSY1(YPL097W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:PES4(YFR023W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PEX29(YDR479C)|FD-Score:4.27|P-value:9.68E-6||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PEX30(YLR324W)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PMT3(YOR321W)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:REC104(YHR157W)|FD-Score:4.57|P-value:2.46E-6||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RMD8(YFR048W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RPS23B(YPR132W)|FD-Score:-3.79|P-value:7.67E-5||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RPS8A(YBL072C)|FD-Score:-3.13|P-value:8.73E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RSM23(YGL129C)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p Gene:RTC4(YNL254C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:SLM6(YBR266C_d)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SSN3(YPL042C)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SSQ1(YLR369W)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:SUV3(YPL029W)|FD-Score:-3.99|P-value:3.26E-5||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TSC3(YBR058C-A)|FD-Score:24.4|P-value:8.79E-132||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UTR2(YEL040W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck Gene:YAR1(YPL239W)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YHR210C(YHR210C_p)|FD-Score:-3.14|P-value:8.42E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YMR130W(YMR130W_p)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Putative protein of unknown function; YMR130W is not an essential gene Gene:YMR178W(YMR178W_p)|FD-Score:5.28|P-value:6.60E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; YMR178W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPL073C(YPL073C_d)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:ACB1(YGR037C)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ARL1(YBR164C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ATG19(YOL082W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles Gene:BCH1(YMR237W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:CBS1(YDL069C)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CDC26(YFR036W)|FD-Score:3.82|P-value:6.67E-5||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CLB2(YPR119W)|FD-Score:4.39|P-value:5.79E-6||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CLB3(YDL155W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:COG8(YML071C)|FD-Score:7.65|P-value:1.04E-14||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUP9(YPL177C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:DCC1(YCL016C)|FD-Score:-4.57|P-value:2.44E-6||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:FAR11(YNL127W)|FD-Score:4.98|P-value:3.20E-7||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FEN1(YCR034W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:GCN3(YKR026C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:HAL1(YPR005C)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:HER2(YMR293C)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:IRC21(YMR073C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:JJJ1(YNL227C)|FD-Score:-3.1|P-value:9.73E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:KRE28(YDR532C)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:LRO1(YNR008W)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MCM16(YPR046W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MDG1(YNL173C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MHR1(YDR296W)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MLS1(YNL117W)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MSY1(YPL097W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:PES4(YFR023W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PEX29(YDR479C)|FD-Score:4.27|P-value:9.68E-6||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PEX30(YLR324W)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PMT3(YOR321W)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:REC104(YHR157W)|FD-Score:4.57|P-value:2.46E-6||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RMD8(YFR048W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RPS23B(YPR132W)|FD-Score:-3.79|P-value:7.67E-5||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RPS8A(YBL072C)|FD-Score:-3.13|P-value:8.73E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RSM23(YGL129C)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p Gene:RTC4(YNL254C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:SLM6(YBR266C_d)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SSN3(YPL042C)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SSQ1(YLR369W)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:SUV3(YPL029W)|FD-Score:-3.99|P-value:3.26E-5||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TSC3(YBR058C-A)|FD-Score:24.4|P-value:8.79E-132||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UTR2(YEL040W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck Gene:YAR1(YPL239W)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YHR210C(YHR210C_p)|FD-Score:-3.14|P-value:8.42E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YMR130W(YMR130W_p)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Putative protein of unknown function; YMR130W is not an essential gene Gene:YMR178W(YMR178W_p)|FD-Score:5.28|P-value:6.60E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; YMR178W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPL073C(YPL073C_d)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR192W10.302.26E-252.37RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YJL033W7.978.02E-162.00HCA4DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis
YBR123C5.971.18E-90.03TFC1One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
YAL001C5.941.41E-90.63TFC3Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
YLR458W_d5.315.51E-80.03YLR458W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis
YPR034W5.286.32E-80.60ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YHR074W4.681.44E-60.08QNS1Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide
YNL131W4.602.15E-60.07TOM22Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes
YIL021W4.523.04E-60.02RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
YDR404C4.503.42E-60.12RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YGR175C4.385.92E-60.08ERG1Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
YHR062C4.308.40E-60.19RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YOR310C4.111.94E-50.11NOP58Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
YHR007C4.013.10E-50.01ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YJL195C_d3.993.24E-50.08YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A24.408.79E-132TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YML071C7.651.04E-14COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YMR178W_p5.286.60E-8YMR178W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; YMR178W is not an essential gene; protein abundance increases in response to DNA replication stress
YGR037C5.151.31E-7ACB1Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
YNL127W4.983.20E-7FAR11Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2
YHR157W4.572.46E-6REC104Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination
YPR119W4.395.79E-6CLB2B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome
YDR479C4.279.68E-6PEX29Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress
YFR023W4.161.56E-5PES4Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication
YNR008W4.052.55E-5LRO1Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase
YFR036W3.826.67E-5CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YOL082W3.591.68E-4ATG19Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles
YBR164C3.512.21E-4ARL1Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor
YPR046W3.442.87E-4MCM16Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3
YPL177C3.383.62E-4CUP9Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_1815
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4870SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.025974TSC3-RPN4
0.4621.80E-309SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0657895TSC3-RPN4
0.4611.27E-308SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.129412TSC3-RPN4
0.4489.42E-290SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.236111TSC3-RPN4
0.4166.55E-246SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.135135TSC3-RPN4
0.4166.80E-246SGTC_1813st051246 8.6 μMTimTec (Natural product derivative library)52919590.0972222TSC3-RPN4
0.4101.06E-237SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.16TSC3-RPN4
0.4084.45E-236SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.1TSC3-RPN4
0.4011.99E-226SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.173913TSC3-RPN4
0.3885.16E-211SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.151163TSC3-RPN4
0.3765.05E-198SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.0875TSC3-RPN4
0.3733.62E-194SGTC_21125488546 200.0 μMChembridge (Fragment library)6007570.0136986TSC3-RPN4
0.3611.51E-181SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.192771TSC3-RPN4
0.3601.62E-180SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.112676TSC3-RPN4
0.3544.45E-173SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.0958904

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1900st06020072.8 μM0.557692688945TimTec (Natural product derivative library)204.221882.67803
SGTC_1905st06020784.5 μM0.5178571948547TimTec (Natural product derivative library)216.232582.94603
SGTC_7844579-0127173 μM0.5178571948547ChemDiv (Drug-like library)216.232582.94603
SGTC_1904st06022578 μM0.44642966595TimTec (Natural product derivative library)204.221882.67803
SGTC_1976st07060963.5 μM0.415385682556TimTec (Natural product derivative library)314.719844.44204
SGTC_1896st05844189 μM0.413793688759TimTec (Natural product derivative library)224.640362.603
SGTC_15447-methoxy-4-methylcoumarin105 μM0.392857390807TimTec (Pure natural product library)190.19532.32903
SGTC_1903st06021178.3 μM0.383333688960TimTec (Natural product derivative library)220.221281.9204
SGTC_1526st05151875 μM0.380952673993TimTec (Pure natural product library)266.677043.44504
SGTC_1934st07472032.6 μM0.37974717250992TimTec (Natural product derivative library)493.981924.65407