st051957

7-methoxy-3,4,8-trimethylchromen-2-one

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1816
Screen concentration 91.6 μM
Source TimTec (Natural product derivative library)
PubChem CID 674121
SMILES CC1=C(C(=O)OC2=C1C=CC(=C2C)OC)C
Standardized SMILES COc1ccc2C(=C(C)C(=O)Oc2c1C)C
Molecular weight 218.2485
ALogP 3.26
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.4
% growth inhibition (Hom. pool) 1.12


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 674121
Download HIP data (tab-delimited text)  (excel)
Gene:DIB1(YPR082C)|FD-Score:3.99|P-value:3.31E-5|Clearance:0.37||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:GPI10(YGL142C)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.02||SGD DESC:Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog Gene:JIP5(YPR169W)|FD-Score:3.37|P-value:3.82E-4|Clearance:0.06||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:MAS2(YHR024C)|FD-Score:3.31|P-value:4.70E-4|Clearance:0.18||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:PHS1(YJL097W)|FD-Score:-3.37|P-value:3.79E-4|Clearance:0||SGD DESC:Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking Gene:RPB3(YIL021W)|FD-Score:3.76|P-value:8.57E-5|Clearance:0.37||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:SEC10(YLR166C)|FD-Score:4.03|P-value:2.76E-5|Clearance:0.37||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SEC63(YOR254C)|FD-Score:3.13|P-value:8.71E-4|Clearance:0.04||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:TFC4(YGR047C)|FD-Score:-3.13|P-value:8.63E-4|Clearance:0||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 Gene:DIB1(YPR082C)|FD-Score:3.99|P-value:3.31E-5|Clearance:0.37||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:GPI10(YGL142C)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.02||SGD DESC:Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog Gene:JIP5(YPR169W)|FD-Score:3.37|P-value:3.82E-4|Clearance:0.06||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:MAS2(YHR024C)|FD-Score:3.31|P-value:4.70E-4|Clearance:0.18||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:PHS1(YJL097W)|FD-Score:-3.37|P-value:3.79E-4|Clearance:0||SGD DESC:Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking Gene:RPB3(YIL021W)|FD-Score:3.76|P-value:8.57E-5|Clearance:0.37||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:SEC10(YLR166C)|FD-Score:4.03|P-value:2.76E-5|Clearance:0.37||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SEC63(YOR254C)|FD-Score:3.13|P-value:8.71E-4|Clearance:0.04||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:TFC4(YGR047C)|FD-Score:-3.13|P-value:8.63E-4|Clearance:0||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 674121
Download HOP data (tab-delimited text)  (excel)
Gene:AFT2(YPL202C)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:ATG12(YBR217W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:BFA1(YJR053W)|FD-Score:-3.72|P-value:9.78E-5||SGD DESC:Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis Gene:CCS1(YMR038C)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CLD1(YGR110W)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:COA4(YLR218C)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:COG8(YML071C)|FD-Score:5.31|P-value:5.58E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:EAF6(YJR082C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ECM22(YLR228C)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:ECM33(YBR078W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ENT2(YLR206W)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus Gene:GCR2(YNL199C)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GDT1(YBR187W_p)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GIS3(YLR094C)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Protein of unknown function Gene:GMC2(YLR445W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:IRS4(YKR019C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KAR9(YPL269W)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:NPR2(YEL062W)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:OXP1(YKL215C)|FD-Score:3.77|P-value:8.04E-5||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PER33(YLR064W)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PES4(YFR023W)|FD-Score:4|P-value:3.11E-5||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:RBS1(YDL189W)|FD-Score:-4.16|P-value:1.57E-5||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:RMD9(YGL107C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RSM24(YDR175C)|FD-Score:3.82|P-value:6.67E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SEF1(YBL066C)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Putative transcription factor, has homolog in Kluyveromyces lactis Gene:SHO1(YER118C)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:THI4(YGR144W)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:TOM70(YNL121C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TRM82(YDR165W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TRP2(YER090W)|FD-Score:5.06|P-value:2.08E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VMA13(YPR036W)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:YAL016C-B(YAL016C-B_d)|FD-Score:4.38|P-value:6.05E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL083C(YBL083C_d)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBT1(YLL048C)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YCK3(YER123W)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDR010C(YDR010C_d)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFR057W(YFR057W_p)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Putative protein of unknown function Gene:YIL067C(YIL067C_p)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YMR074C(YMR074C)|FD-Score:-3.12|P-value:8.90E-4||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR193C-A(YMR193C-A_d)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR186W(YOR186W_p)|FD-Score:8.19|P-value:1.33E-16||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:AFT2(YPL202C)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:ATG12(YBR217W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:BFA1(YJR053W)|FD-Score:-3.72|P-value:9.78E-5||SGD DESC:Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis Gene:CCS1(YMR038C)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CLD1(YGR110W)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:COA4(YLR218C)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:COG8(YML071C)|FD-Score:5.31|P-value:5.58E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:EAF6(YJR082C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ECM22(YLR228C)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:ECM33(YBR078W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ENT2(YLR206W)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus Gene:GCR2(YNL199C)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GDT1(YBR187W_p)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GIS3(YLR094C)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Protein of unknown function Gene:GMC2(YLR445W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:IRS4(YKR019C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KAR9(YPL269W)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:NPR2(YEL062W)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:OXP1(YKL215C)|FD-Score:3.77|P-value:8.04E-5||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PER33(YLR064W)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PES4(YFR023W)|FD-Score:4|P-value:3.11E-5||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:RBS1(YDL189W)|FD-Score:-4.16|P-value:1.57E-5||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:RMD9(YGL107C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RSM24(YDR175C)|FD-Score:3.82|P-value:6.67E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SEF1(YBL066C)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Putative transcription factor, has homolog in Kluyveromyces lactis Gene:SHO1(YER118C)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:THI4(YGR144W)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:TOM70(YNL121C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TRM82(YDR165W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TRP2(YER090W)|FD-Score:5.06|P-value:2.08E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VMA13(YPR036W)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:YAL016C-B(YAL016C-B_d)|FD-Score:4.38|P-value:6.05E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL083C(YBL083C_d)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBT1(YLL048C)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YCK3(YER123W)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDR010C(YDR010C_d)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFR057W(YFR057W_p)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Putative protein of unknown function Gene:YIL067C(YIL067C_p)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YMR074C(YMR074C)|FD-Score:-3.12|P-value:8.90E-4||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR193C-A(YMR193C-A_d)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR186W(YOR186W_p)|FD-Score:8.19|P-value:1.33E-16||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR166C4.032.76E-50.37SEC10Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion
YPR082C3.993.31E-50.37DIB117-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein
YIL021W3.768.57E-50.37RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
YGL142C3.383.58E-40.02GPI10Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog
YPR169W3.373.82E-40.06JIP5Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein
YHR024C3.314.70E-40.18MAS2Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YOR254C3.138.71E-40.04SEC63Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YPR085C3.090.001010.02ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
YKL172W3.060.001090.17EBP2Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
YDR238C2.890.001910.00SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YDL196W_d2.890.001910.06YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YPL020C2.830.002330.10ULP1Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions
YGR073C_d2.730.003180.10YGR073C_dDubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein
YPL211W2.630.004280.01NIP7Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p
YKR071C2.620.004430.17DRE2Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR186W_p8.191.33E-16YOR186W_pPutative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication
YML071C5.315.58E-8COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YER090W5.062.08E-7TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YMR038C4.741.06E-6CCS1Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress
YER123W4.552.74E-6YCK3Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway
YAL016C-B_d4.386.05E-6YAL016C-B_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPR036W4.211.29E-5VMA13Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase
YLR094C4.111.96E-5GIS3Protein of unknown function
YEL062W4.013.07E-5NPR2Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline
YFR023W4.003.11E-5PES4Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication
YDR175C3.826.67E-5RSM24Mitochondrial ribosomal protein of the small subunit
YKL215C3.778.04E-5OXP15-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
YDR165W3.711.03E-4TRM82Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
YKR019C3.711.03E-4IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YNL121C3.671.20E-4TOM70Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1816
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1604.27E-35SGTC_22557941087 197.4 μMChembridge (Fragment library)9355610.0806452
0.1431.55E-28SGTC_507nimesulide 162.0 μMMiscellaneous44950.116667
0.1361.60E-25SGTC_2231micatex 6.9 μMMiscellaneous207370.116279
0.1313.95E-24SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.254545
0.1286.03E-23SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.156863
0.1271.13E-22SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.103448
0.1241.30E-21SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0408163
0.1194.02E-20SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.12963
0.1194.90E-20SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.0769231
0.1196.04E-20SGTC_1671st016596 74.5 μMTimTec (Natural product derivative library)28291670.0882353
0.1188.60E-20SGTC_1669st015249 58.4 μMTimTec (Natural product derivative library)7227930.131148Golgi
0.1133.18E-18SGTC_18233-hydroxy-2-naphthoic acid 106.0 μMTimTec (Natural product derivative library)71040.0833333
0.1119.07E-18SGTC_1838st055369 84.5 μMTimTec (Natural product derivative library)6760360.22449
0.1102.14E-17SGTC_1813st051246 8.6 μMTimTec (Natural product derivative library)52919590.0714286TSC3-RPN4
0.1103.02E-17SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.111111

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_7234182-0001115 μM0.536585744821ChemDiv (Drug-like library)218.248463.27203
SGTC_1901st06021658.6 μM0.372093688792TimTec (Natural product derivative library)204.221882.5203
SGTC_13973532-019020.1 μM0.3555565322280ChemDiv (Drug-like library)230.259163.57713
SGTC_1782st03942380.64 μM0.3541672068169TimTec (Natural product derivative library)246.301624.10403
SGTC_1518st03856790.8 μM0.32558177966TimTec (Pure natural product library)220.221282.31304
SGTC_1867st05737264 μM0.3125688837TimTec (Natural product derivative library)312.316643.08705
SGTC_15447-methoxy-4-methylcoumarin105 μM0.311111390807TimTec (Pure natural product library)190.19532.32903
SGTC_1866st05763864 μM0.306122688802TimTec (Natural product derivative library)312.316643.08705
SGTC_1848st05600316.4 μM0.304348146492TimTec (Natural product derivative library)252.264683.1203
SGTC_1853st05671091.2 μM0.295455269167TimTec (Natural product derivative library)219.236521.66613amide catabolism