st052116

1-[2-(4-chlorophenoxy)ethoxymethyl]-6-methylpyrimidine-2,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1817
Screen concentration 64.4 μM
Source TimTec (Natural product derivative library)
PubChem CID 4361398
SMILES CC1=CC(=O)NC(=O)N1COCCOC2=CC=C(C=C2)Cl
Standardized SMILES CC1=CC(=O)NC(=O)N1COCCOc2ccc(Cl)cc2
Molecular weight 310.7329
ALogP 1.86
H-bond donor count 1
H-bond acceptor count 4
Response signature RNA processing & uracil transport

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.4
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4361398
Download HIP data (tab-delimited text)  (excel)
Gene:BIG1(YHR101C)|FD-Score:3.11|P-value:9.26E-4|Clearance:0.05||SGD DESC:Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan Gene:KEI1(YDR367W)|FD-Score:3.17|P-value:7.67E-4|Clearance:0.05||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MVD1(YNR043W)|FD-Score:-3.14|P-value:8.58E-4|Clearance:0||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:PCF11(YDR228C)|FD-Score:3.44|P-value:2.92E-4|Clearance:0.02||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PGI1(YBR196C)|FD-Score:3.2|P-value:6.92E-4|Clearance:0.03||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:RHO1(YPR165W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.17||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RRP15(YPR143W)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.22||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RRP45(YDR280W)|FD-Score:4.43|P-value:4.80E-6|Clearance:0.82||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SUP45(YBR143C)|FD-Score:-4.11|P-value:2.02E-5|Clearance:0||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:YJL086C(YJL086C_d)|FD-Score:-3.86|P-value:5.67E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YNL114C(YNL114C_d)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:BIG1(YHR101C)|FD-Score:3.11|P-value:9.26E-4|Clearance:0.05||SGD DESC:Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan Gene:KEI1(YDR367W)|FD-Score:3.17|P-value:7.67E-4|Clearance:0.05||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MVD1(YNR043W)|FD-Score:-3.14|P-value:8.58E-4|Clearance:0||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:PCF11(YDR228C)|FD-Score:3.44|P-value:2.92E-4|Clearance:0.02||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PGI1(YBR196C)|FD-Score:3.2|P-value:6.92E-4|Clearance:0.03||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:RHO1(YPR165W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.17||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RRP15(YPR143W)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.22||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RRP45(YDR280W)|FD-Score:4.43|P-value:4.80E-6|Clearance:0.82||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SUP45(YBR143C)|FD-Score:-4.11|P-value:2.02E-5|Clearance:0||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:YJL086C(YJL086C_d)|FD-Score:-3.86|P-value:5.67E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YNL114C(YNL114C_d)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4361398
Download HOP data (tab-delimited text)  (excel)
Gene:ATG27(YJL178C)|FD-Score:4.81|P-value:7.43E-7||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:CDC26(YFR036W)|FD-Score:4.81|P-value:7.74E-7||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CUE5(YOR042W)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:CYT1(YOR065W)|FD-Score:5.21|P-value:9.35E-8||SGD DESC:Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex Gene:DSE2(YHR143W)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DUF1(YOL087C)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ECM38(YLR299W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation Gene:EDE1(YBL047C)|FD-Score:7.66|P-value:9.59E-15||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:EMC3(YKL207W)|FD-Score:-3.19|P-value:7.06E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 Gene:FUR4(YBR021W)|FD-Score:11.5|P-value:3.95E-31||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCN5(YGR252W)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:HFM1(YGL251C)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HTD2(YHR067W)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:HXT2(YMR011W)|FD-Score:4|P-value:3.22E-5||SGD DESC:High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:ICY2(YPL250C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:IME4(YGL192W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:ISC10(YER180C)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:MAC1(YMR021C)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDS3(YGL197W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MZM1(YDR493W)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NUM1(YDR150W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OMS1(YDR316W)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:POL32(YJR043C)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:RAS2(YNL098C)|FD-Score:3.97|P-value:3.63E-5||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RNH70(YGR276C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:ROM2(YLR371W)|FD-Score:4.48|P-value:3.73E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPA14(YDR156W)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:RNA polymerase I subunit A14 Gene:RTC2(YBR147W)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:RTG2(YGL252C)|FD-Score:-3.46|P-value:2.65E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:SHE1(YBL031W)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SIF2(YBR103W)|FD-Score:3.83|P-value:6.40E-5||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SNF2(YOR290C)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNX4(YJL036W)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPS2(YDR522C)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SPT21(YMR179W)|FD-Score:-3.75|P-value:8.75E-5||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:UBA4(YHR111W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UBP16(YPL072W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria Gene:UBP6(YFR010W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:YBL029W(YBL029W_p)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Non-essential protein of unknown function Gene:YBR027C(YBR027C_d)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR203W(YDR203W_d)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL032W(YFL032W_d)|FD-Score:5.06|P-value:2.05E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Putative protein of unknown function Gene:YMR221C(YMR221C_p)|FD-Score:3.78|P-value:7.75E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YND1(YER005W)|FD-Score:3.9|P-value:4.87E-5||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YPL068C(YPL068C_p)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:ATG27(YJL178C)|FD-Score:4.81|P-value:7.43E-7||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:CDC26(YFR036W)|FD-Score:4.81|P-value:7.74E-7||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CUE5(YOR042W)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:CYT1(YOR065W)|FD-Score:5.21|P-value:9.35E-8||SGD DESC:Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex Gene:DSE2(YHR143W)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DUF1(YOL087C)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ECM38(YLR299W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation Gene:EDE1(YBL047C)|FD-Score:7.66|P-value:9.59E-15||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:EMC3(YKL207W)|FD-Score:-3.19|P-value:7.06E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 Gene:FUR4(YBR021W)|FD-Score:11.5|P-value:3.95E-31||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCN5(YGR252W)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:HFM1(YGL251C)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HTD2(YHR067W)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:HXT2(YMR011W)|FD-Score:4|P-value:3.22E-5||SGD DESC:High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:ICY2(YPL250C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:IME4(YGL192W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:ISC10(YER180C)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:MAC1(YMR021C)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDS3(YGL197W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MZM1(YDR493W)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NUM1(YDR150W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OMS1(YDR316W)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:POL32(YJR043C)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:RAS2(YNL098C)|FD-Score:3.97|P-value:3.63E-5||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RNH70(YGR276C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:ROM2(YLR371W)|FD-Score:4.48|P-value:3.73E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPA14(YDR156W)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:RNA polymerase I subunit A14 Gene:RTC2(YBR147W)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:RTG2(YGL252C)|FD-Score:-3.46|P-value:2.65E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:SHE1(YBL031W)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SIF2(YBR103W)|FD-Score:3.83|P-value:6.40E-5||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SNF2(YOR290C)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNX4(YJL036W)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPS2(YDR522C)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SPT21(YMR179W)|FD-Score:-3.75|P-value:8.75E-5||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:UBA4(YHR111W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UBP16(YPL072W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria Gene:UBP6(YFR010W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:YBL029W(YBL029W_p)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Non-essential protein of unknown function Gene:YBR027C(YBR027C_d)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR203W(YDR203W_d)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL032W(YFL032W_d)|FD-Score:5.06|P-value:2.05E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Putative protein of unknown function Gene:YMR221C(YMR221C_p)|FD-Score:3.78|P-value:7.75E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YND1(YER005W)|FD-Score:3.9|P-value:4.87E-5||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YPL068C(YPL068C_p)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR280W4.434.80E-60.82RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YPR165W3.611.55E-40.17RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YDR228C3.442.92E-40.02PCF11mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
YPR143W3.423.11E-40.22RRP15Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
YBR196C3.206.92E-40.03PGI1Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
YDR367W3.177.67E-40.06KEI1Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
YHR101C3.119.26E-40.05BIG1Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan
YOR260W3.070.001080.00GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YGR005C3.060.001100.10TFG2TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30
YJL174W2.960.001520.01KRE9Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects
YOR250C2.960.001569.14E-4CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YDR396W_d2.950.001560.09YDR396W_dDubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex
YCL043C2.870.002060.01PDI1Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
YLR029C2.860.002120.01RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YNL282W2.850.002190.10POP3Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR021W11.503.95E-31FUR4Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
YBL047C7.669.59E-15EDE1Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death
YOR065W5.219.35E-8CYT1Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
YFL032W_d5.062.05E-7YFL032W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
YJL178C4.817.43E-7ATG27Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site
YFR036W4.817.74E-7CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YLR371W4.483.73E-6ROM2GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p
YDR522C4.191.41E-5SPS2Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component
YOR290C4.161.62E-5SNF2Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p
YJR043C4.042.63E-5POL32Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p
YMR011W4.003.22E-5HXT2High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose
YNL098C3.973.63E-5RAS2GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes
YHR143W3.944.02E-5DSE2Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP
YER005W3.904.87E-5YND1Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity
YDR316W3.904.89E-5OMS1Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations

GO enrichment analysis for SGTC_1817
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2264.08E-69SGTC_1814st052110 31.5 μMTimTec (Natural product derivative library)6741630.4RNA processing & uracil transport
0.2162.30E-63SGTC_23519026858 72.3 μMChembridge (Fragment library)14093000.285714RNA processing & uracil transport
0.1991.65E-53SGTC_23939031399 185.9 μMChembridge (Fragment library)15266230.285714RNA processing & uracil transport
0.1951.64E-51SGTC_1471k673-0312 37.0 μMChemDiv (Drug-like library)240998840.0632911RNA processing & uracil transport
0.1738.41E-41SGTC_20825219693 150.0 μMChembridge (Fragment library)28346490.0895522RNA processing & uracil transport
0.1682.05E-38SGTC_22977971671 152.3 μMChembridge (Fragment library)9090160.181818RNA processing & uracil transport
0.1583.47E-34SGTC_8093998-0021 90.0 μMChemDiv (Drug-like library)7154850.128571RNA processing & uracil transport
0.1477.60E-30SGTC_1167k781-0610 148.0 μMChemDiv (Drug-like library)45251690.0821918RNA processing & uracil transport
0.1461.24E-29SGTC_3051682-2595 20.8 μMChemDiv (Drug-like library)2260330.0909091RNA processing & uracil transport
0.1331.03E-24SGTC_9231900-2434 73.3 μMChemDiv (Drug-like library)59105700.0617284RNA processing & uracil transport
0.1316.98E-24SGTC_33559151058 44.0 μMChembridge (Drug-like library)170157280.0779221RNA processing & uracil transport
0.1262.01E-22SGTC_486niguldipine 82.0 μMMiscellaneous12360.12766amide catabolism
0.1231.90E-21SGTC_22927671323 168.1 μMChembridge (Fragment library)9375040.166667RNA processing & uracil transport
0.1233.11E-21SGTC_530973-0023 40.0 μMChemDiv (Drug-like library)6747680.117647RNA processing & uracil transport
0.1132.30E-18SGTC_452diindolylmethane 8.0 μMICCB bioactive library30710.0701754RNA processing & uracil transport

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1814st05211031.54 μM0.4674163TimTec (Natural product derivative library)345.57641.94313RNA processing & uracil transport
SGTC_2232chlorophetanol41.54 μM0.35555615907Miscellaneous172.608861.93812TRP & mitochondrial translation
SGTC_6033794-002345.9 μM0.3050853802260ChemDiv (Drug-like library)356.759823.09624
SGTC_2351902685872.31 μM0.2857141409300Chembridge (Fragment library)231.250520.87923RNA processing & uracil transport
SGTC_23939031399185.94 μM0.2857141526623Chembridge (Fragment library)218.212-0.59324RNA processing & uracil transport
SGTC_1994063-002441.6 μM0.280702306215ChemDiv (Drug-like library)311.783863.31414
SGTC_22206782180200 μM0.275862833803Chembridge (Fragment library)207.18606-0.26124RNA processing & uracil transport
SGTC_3310913022944.14 μM0.2741947761258Chembridge (Drug-like library)289.756743.8212Golgi
SGTC_3127912694649.47 μM0.27272727243249Chembridge (Drug-like library)223.698663.0212ERG2
SGTC_1737st03555769.7 μM0.2711865716916TimTec (Natural product derivative library)286.75284.69802