st053465

6-bromo-N-methyl-2-oxochromene-3-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1821
Screen concentration 65.6 μM
Source TimTec (Natural product derivative library)
PubChem CID 675086
SMILES CNC(=O)C1=CC2=C(C=CC(=C2)Br)OC1=O
Standardized SMILES CNC(=O)C1=Cc2cc(Br)ccc2OC1=O
Molecular weight 282.0901
ALogP 1.82
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.55
% growth inhibition (Hom. pool) 2.21


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 675086
Download HIP data (tab-delimited text)  (excel)
Gene:ENP1(YBR247C)|FD-Score:3.29|P-value:4.94E-4|Clearance:0.03||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:FAS1(YKL182W)|FD-Score:3.71|P-value:1.05E-4|Clearance:0.15||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:MIM1(YOL026C)|FD-Score:4.41|P-value:5.10E-6|Clearance:0.39||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:PRP19(YLL036C)|FD-Score:-3.11|P-value:9.30E-4|Clearance:0||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RAD53(YPL153C)|FD-Score:4.02|P-value:2.89E-5|Clearance:0.31||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:SDO1(YLR022C)|FD-Score:3.3|P-value:4.77E-4|Clearance:0.01||SGD DESC:Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes Gene:SLD5(YDR489W)|FD-Score:3.26|P-value:5.49E-4|Clearance:0.34||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SMX3(YPR182W)|FD-Score:3.56|P-value:1.87E-4|Clearance:0.1||SGD DESC:Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F Gene:YBL077W(YBL077W_d)|FD-Score:3.46|P-value:2.74E-4|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:ENP1(YBR247C)|FD-Score:3.29|P-value:4.94E-4|Clearance:0.03||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:FAS1(YKL182W)|FD-Score:3.71|P-value:1.05E-4|Clearance:0.15||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:MIM1(YOL026C)|FD-Score:4.41|P-value:5.10E-6|Clearance:0.39||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:PRP19(YLL036C)|FD-Score:-3.11|P-value:9.30E-4|Clearance:0||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RAD53(YPL153C)|FD-Score:4.02|P-value:2.89E-5|Clearance:0.31||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:SDO1(YLR022C)|FD-Score:3.3|P-value:4.77E-4|Clearance:0.01||SGD DESC:Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes Gene:SLD5(YDR489W)|FD-Score:3.26|P-value:5.49E-4|Clearance:0.34||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SMX3(YPR182W)|FD-Score:3.56|P-value:1.87E-4|Clearance:0.1||SGD DESC:Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F Gene:YBL077W(YBL077W_d)|FD-Score:3.46|P-value:2.74E-4|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 675086
Download HOP data (tab-delimited text)  (excel)
Gene:AIR2(YDL175C)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:ALT1(YLR089C)|FD-Score:3.81|P-value:6.94E-5||SGD DESC:Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive Gene:ASE1(YOR058C)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:ATG23(YLR431C)|FD-Score:3.84|P-value:6.05E-5||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:BNA7(YDR428C)|FD-Score:-3.84|P-value:6.13E-5||SGD DESC:Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine Gene:CUE5(YOR042W)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:CUS2(YNL286W)|FD-Score:-3.94|P-value:4.10E-5||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:DEP1(YAL013W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DOT6(YER088C)|FD-Score:3.74|P-value:9.09E-5||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ENT2(YLR206W)|FD-Score:-4.49|P-value:3.51E-6||SGD DESC:Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus Gene:HSL1(YKL101W)|FD-Score:4.8|P-value:7.78E-7||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:HXK1(YFR053C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:ICL1(YER065C)|FD-Score:-3.91|P-value:4.65E-5||SGD DESC:Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose Gene:ICY2(YPL250C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:MSH3(YCR092C)|FD-Score:3.83|P-value:6.33E-5||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:NMA1(YLR328W)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:OAF3(YKR064W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:PAP2(YOL115W)|FD-Score:4.9|P-value:4.81E-7||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PCS60(YBR222C)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase Gene:PHM6(YDR281C)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PLB2(YMR006C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:RAD57(YDR004W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RAS2(YNL098C)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RNP1(YLL046C)|FD-Score:-3.8|P-value:7.31E-5||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPS4A(YJR145C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RTG3(YBL103C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways Gene:SKI3(YPR189W)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SOH1(YGL127C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SPT3(YDR392W)|FD-Score:-3.81|P-value:6.84E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:TGS1(YPL157W)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TRE1(YPL176C)|FD-Score:-4.49|P-value:3.57E-6||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRM82(YDR165W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:UBP6(YFR010W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:UTH1(YKR042W)|FD-Score:5.77|P-value:3.93E-9||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:YDR535C(YDR535C_d)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YER135C(YER135C_d)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YJL218W(YJL218W_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YML034C-A(YML034C-A_d)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL247C(YPL247C_p)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:AIR2(YDL175C)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:ALT1(YLR089C)|FD-Score:3.81|P-value:6.94E-5||SGD DESC:Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive Gene:ASE1(YOR058C)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:ATG23(YLR431C)|FD-Score:3.84|P-value:6.05E-5||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:BNA7(YDR428C)|FD-Score:-3.84|P-value:6.13E-5||SGD DESC:Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine Gene:CUE5(YOR042W)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:CUS2(YNL286W)|FD-Score:-3.94|P-value:4.10E-5||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:DEP1(YAL013W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DOT6(YER088C)|FD-Score:3.74|P-value:9.09E-5||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ENT2(YLR206W)|FD-Score:-4.49|P-value:3.51E-6||SGD DESC:Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus Gene:HSL1(YKL101W)|FD-Score:4.8|P-value:7.78E-7||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:HXK1(YFR053C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:ICL1(YER065C)|FD-Score:-3.91|P-value:4.65E-5||SGD DESC:Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose Gene:ICY2(YPL250C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:MSH3(YCR092C)|FD-Score:3.83|P-value:6.33E-5||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:NMA1(YLR328W)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:OAF3(YKR064W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:PAP2(YOL115W)|FD-Score:4.9|P-value:4.81E-7||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PCS60(YBR222C)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase Gene:PHM6(YDR281C)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PLB2(YMR006C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:RAD57(YDR004W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RAS2(YNL098C)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RNP1(YLL046C)|FD-Score:-3.8|P-value:7.31E-5||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPS4A(YJR145C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RTG3(YBL103C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways Gene:SKI3(YPR189W)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SOH1(YGL127C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SPT3(YDR392W)|FD-Score:-3.81|P-value:6.84E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:TGS1(YPL157W)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TRE1(YPL176C)|FD-Score:-4.49|P-value:3.57E-6||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRM82(YDR165W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:UBP6(YFR010W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:UTH1(YKR042W)|FD-Score:5.77|P-value:3.93E-9||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:YDR535C(YDR535C_d)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YER135C(YER135C_d)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YJL218W(YJL218W_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YML034C-A(YML034C-A_d)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL247C(YPL247C_p)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOL026C4.415.10E-60.39MIM1Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex
YPL153C4.022.89E-50.32RAD53Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication
YKL182W3.711.05E-40.15FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YPR182W3.561.87E-40.10SMX3Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F
YBL077W_d3.462.74E-40.15YBL077W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C
YLR022C3.304.77E-40.01SDO1Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes
YBR247C3.294.94E-40.03ENP1Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus
YDR489W3.265.49E-40.34SLD5Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YOR176W2.930.001700.09HEM15Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway
YDR396W_d2.840.002280.15YDR396W_dDubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex
YIR008C2.690.003590.03PRI1Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YDR478W2.660.003900.02SNM1Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
YKL028W2.640.004150.08TFA1TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
YGR074W2.560.005308.88E-4SMD1Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress
YKL042W2.560.005310.05SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKR042W5.773.93E-9UTH1Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
YOL115W4.904.81E-7PAP2Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p
YKL101W4.807.78E-7HSL1Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p
YLR328W3.993.27E-5NMA1Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication
YPR189W3.993.31E-5SKI3Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay
YLR431C3.846.05E-5ATG23Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p
YCR092C3.836.33E-5MSH3Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability
YLR089C3.816.94E-5ALT1Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive
YER088C3.749.09E-5DOT6Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YFR053C3.621.49E-4HXK1Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication
YDR535C_d3.601.61E-4YDR535C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene.
YFR010W3.581.74E-4UBP6Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance
YJL218W_p3.571.78E-4YJL218W_pPutative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene
YKR064W3.542.02E-4OAF3Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress
YDL175C3.532.04E-4AIR2RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1821
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1157.52E-19SGTC_2261,3-diallylurea 8.3 mMMiscellaneous745370.0851064
0.0681.62E-7SGTC_22216807166 181.1 μMChembridge (Fragment library)6749250.109375
0.0656.73E-7SGTC_1663st014140 55.4 μMTimTec (Natural product derivative library)42071200.13513560S ribosome export
0.0631.39E-6SGTC_30359091303 49.5 μMChembridge (Drug-like library)252370020.126984
0.0612.45E-6SGTC_2417cerulenin 830.0 nMICCB bioactive library52820540.0806452
0.0613.15E-6SGTC_1798st045719 61.0 μMTimTec (Natural product derivative library)39801860.0886076
0.0588.31E-6SGTC_29439068529 45.3 μMYeast Active Library32706340.196721
0.0526.55E-5SGTC_32339132340 49.5 μMChembridge (Drug-like library)176829500.126761
0.0491.79E-4SGTC_28307992007 71.4 μMChembridge (Drug-like library)19520750.0666667
0.0473.11E-4SGTC_2643anethole 100.0 μMMicrosource (Natural product library)6375630.0784314
0.0447.38E-4SGTC_7891309-1135 70.6 μMChemDiv (Drug-like library)30924240.119403ERG2
0.0448.08E-4SGTC_32479135149 49.5 μMChembridge (Drug-like library)89344360.0714286DNA damage response
0.0439.25E-4SGTC_31049120930 49.5 μMChembridge (Drug-like library)170148360.0704225
0.0439.74E-4SGTC_15924',5'-dihydroxyflavone 8.8 μMTimTec (Pure natural product library)6886690.16393460S ribosome export
0.0420.00131SGTC_3531988-1175 58.3 μMChemDiv (Drug-like library)27883570.147541unfolded protein response

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1871st05744867.3 μM0.622222691747TimTec (Natural product derivative library)297.101462.81204
SGTC_1914st06236772.6 μM0.5978205TimTec (Natural product derivative library)275.299781.98214
SGTC_1628st00425277.7 μM0.411765692968TimTec (Natural product derivative library)257.28452.4713
SGTC_22047233102200 μM0.4117652172480Chembridge (Fragment library)229.231341.6841360S ribosome export
SGTC_1920st07572845.7 μM0.4098363400306TimTec (Natural product derivative library)314.292763.2615
SGTC_1971st06942963.5 μM0.41246522TimTec (Natural product derivative library)280.278082.5191460S ribosome export
SGTC_8810833-0569170 μM0.3888895418700ChemDiv (Drug-like library)306.315362.68414
SGTC_1964st07691461 μM0.33333324208661TimTec (Natural product derivative library)327.761663.82714RPP1 & pyrimidine depletion
SGTC_1862st0575133.81 μM0.3269235393163TimTec (Natural product derivative library)317.134163.88413
SGTC_1911st06020874.3 μM0.326923688783TimTec (Natural product derivative library)269.091362.68403