st055811

[4-[(E)-3-(3-chlorophenyl)prop-2-enoyl]phenyl] 3-nitrobenzoate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1828
Screen concentration 49.0 μM
Source TimTec (Natural product derivative library)
PubChem CID 5877589
SMILES C1=CC(=CC(=C1)Cl)C=CC(=O)C2=CC=C(C=C2)OC(=O)C3=CC(=CC=C3)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1cccc(c1)C(=O)Oc2ccc(cc2)C(=O)C=Cc3cccc(Cl)c3
Molecular weight 407.8033
ALogP 5.69
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.37
% growth inhibition (Hom. pool) 3.17


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5877589
Download HIP data (tab-delimited text)  (excel)
Gene:DIB1(YPR082C)|FD-Score:-3.4|P-value:3.37E-4|Clearance:0||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:FAD1(YDL045C)|FD-Score:-3.63|P-value:1.39E-4|Clearance:0||SGD DESC:Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin Gene:GPI18(YBR004C)|FD-Score:3.92|P-value:4.44E-5|Clearance:0.85||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:HAS1(YMR290C)|FD-Score:4.48|P-value:3.78E-6|Clearance:0.85||SGD DESC:ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles Gene:SEC5(YDR166C)|FD-Score:-3.11|P-value:9.44E-4|Clearance:0||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:DIB1(YPR082C)|FD-Score:-3.4|P-value:3.37E-4|Clearance:0||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:FAD1(YDL045C)|FD-Score:-3.63|P-value:1.39E-4|Clearance:0||SGD DESC:Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin Gene:GPI18(YBR004C)|FD-Score:3.92|P-value:4.44E-5|Clearance:0.85||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:HAS1(YMR290C)|FD-Score:4.48|P-value:3.78E-6|Clearance:0.85||SGD DESC:ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles Gene:SEC5(YDR166C)|FD-Score:-3.11|P-value:9.44E-4|Clearance:0||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5877589
Download HOP data (tab-delimited text)  (excel)
Gene:AAD3(YCR107W)|FD-Score:-3.72|P-value:9.94E-5||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:APA1(YCL050C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication Gene:APL5(YPL195W)|FD-Score:4.56|P-value:2.54E-6||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APM1(YPL259C)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ARR2(YPR200C)|FD-Score:-3.89|P-value:5.08E-5||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:COG7(YGL005C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ECM10(YEL030W)|FD-Score:5.21|P-value:9.46E-8||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:FAA4(YMR246W)|FD-Score:4.28|P-value:9.17E-6||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FRA1(YLL029W)|FD-Score:-3.11|P-value:9.32E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:HOM6(YJR139C)|FD-Score:3.3|P-value:4.92E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOR7(YMR251W-A)|FD-Score:5.32|P-value:5.33E-8||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HUT1(YPL244C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 Gene:ILV1(YER086W)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:KAP120(YPL125W)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:LAG2(YOL025W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:MRN1(YPL184C)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MSS2(YDL107W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p Gene:NOP6(YDL213C)|FD-Score:-4.4|P-value:5.37E-6||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:NPC2(YDL046W)|FD-Score:3.86|P-value:5.72E-5||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:PIR3(YKL163W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:RBS1(YDL189W)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:REH1(YLR387C)|FD-Score:-3.12|P-value:9.02E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:SPE4(YLR146C)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:UBP8(YMR223W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B Gene:UME6(YDR207C)|FD-Score:-4.56|P-value:2.60E-6||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:URC2(YDR520C_p)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:YBR197C(YBR197C_p)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YGL082W(YGL082W_p)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene Gene:YIL060W(YIL060W_p)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YKL133C(YKL133C_p)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YLR280C(YLR280C_d)|FD-Score:-3.95|P-value:3.88E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR158W-B(YMR158W-B_d)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C Gene:YNL200C(YNL200C)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR225W(YOR225W_d)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL277C(YPL277C_p)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Putative protein of unknown function; localized to the membranes; gene expression regulated by copper levels Gene:YSP3(YOR003W)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Putative precursor to the subtilisin-like protease III Gene:AAD3(YCR107W)|FD-Score:-3.72|P-value:9.94E-5||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:APA1(YCL050C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication Gene:APL5(YPL195W)|FD-Score:4.56|P-value:2.54E-6||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APM1(YPL259C)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ARR2(YPR200C)|FD-Score:-3.89|P-value:5.08E-5||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:COG7(YGL005C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ECM10(YEL030W)|FD-Score:5.21|P-value:9.46E-8||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:FAA4(YMR246W)|FD-Score:4.28|P-value:9.17E-6||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FRA1(YLL029W)|FD-Score:-3.11|P-value:9.32E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:HOM6(YJR139C)|FD-Score:3.3|P-value:4.92E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOR7(YMR251W-A)|FD-Score:5.32|P-value:5.33E-8||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HUT1(YPL244C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 Gene:ILV1(YER086W)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:KAP120(YPL125W)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:LAG2(YOL025W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:MRN1(YPL184C)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MSS2(YDL107W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p Gene:NOP6(YDL213C)|FD-Score:-4.4|P-value:5.37E-6||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:NPC2(YDL046W)|FD-Score:3.86|P-value:5.72E-5||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:PIR3(YKL163W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:RBS1(YDL189W)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:REH1(YLR387C)|FD-Score:-3.12|P-value:9.02E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:SPE4(YLR146C)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:UBP8(YMR223W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B Gene:UME6(YDR207C)|FD-Score:-4.56|P-value:2.60E-6||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:URC2(YDR520C_p)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:YBR197C(YBR197C_p)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YGL082W(YGL082W_p)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene Gene:YIL060W(YIL060W_p)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YKL133C(YKL133C_p)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YLR280C(YLR280C_d)|FD-Score:-3.95|P-value:3.88E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR158W-B(YMR158W-B_d)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C Gene:YNL200C(YNL200C)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR225W(YOR225W_d)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL277C(YPL277C_p)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Putative protein of unknown function; localized to the membranes; gene expression regulated by copper levels Gene:YSP3(YOR003W)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Putative precursor to the subtilisin-like protease III

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR290C4.483.78E-60.85HAS1ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles
YBR004C3.924.44E-50.85GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YER172C3.070.001080.01BRR2RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
YLR101C_d3.060.001120.29YLR101C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W
YMR240C2.770.002830.22CUS1Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p
YNL232W2.550.005420.05CSL4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
YDL103C2.500.006170.01QRI1UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
YJL035C2.490.006400.17TAD2Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs
YKL082C2.320.010300.01RRP14Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family
YLR198C_d2.300.010600.10YLR198C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W
YLR272C2.210.013700.02YCS4Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YGR251W2.180.014400.02NOP19Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes
YNL103W2.170.015200.04MET4Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p
YDL108W2.130.016700.05KIN28Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters
YDR498C2.070.019000.00SEC20Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR251W-A5.325.33E-8HOR7Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication
YEL030W5.219.46E-8ECM10Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication
YPL195W4.562.54E-6APL5Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function
YMR246W4.289.17E-6FAA4Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication
YMR158W-B_d4.261.04E-5YMR158W-B_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C
YIL060W_p4.231.15E-5YIL060W_pPutative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene
YPL259C3.983.49E-5APM1Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting
YNL200C3.934.29E-5YNL200CNADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YGL082W_p3.904.89E-5YGL082W_pPutative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene
YDL046W3.865.72E-5NPC2Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes
YGL005C3.581.71E-4COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YOR003W3.571.82E-4YSP3Putative precursor to the subtilisin-like protease III
YBR197C_p3.522.16E-4YBR197C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication
YKL163W3.502.31E-4PIR3O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication
YPL184C3.472.65E-4MRN1RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation

GO enrichment analysis for SGTC_1828
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0945.45E-13SGTC_1832st054113 55.0 μMTimTec (Natural product derivative library)12512670.127907
0.0831.77E-10SGTC_492tmb-8 126.0 μMICCB bioactive library54940.116883
0.0731.83E-8SGTC_1954234-0266 16.7 μMChemDiv (Drug-like library)23033540.109589
0.0672.12E-7SGTC_1871st057448 67.3 μMTimTec (Natural product derivative library)6917470.116883
0.0656.74E-7SGTC_33429147791 14.5 μMChembridge (Drug-like library)18381680.0898876
0.0621.75E-6SGTC_21876125751 200.0 μMChembridge (Fragment library)8744980.0933333RSC complex & mRNA processing
0.0612.95E-6SGTC_1913st060213 96.1 μMTimTec (Natural product derivative library)6889590.0789474
0.0594.83E-6SGTC_2970986-0032 24.0 μMChemDiv (Drug-like library)28474250.12162260S ribosome export
0.0595.24E-6SGTC_22687933382 153.8 μMChembridge (Fragment library)9350250.0493827
0.0587.16E-6SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.103448Golgi
0.0589.68E-6SGTC_13863228-0095 33.8 μMChemDiv (Drug-like library)8701810.0804598
0.0561.52E-5SGTC_900870-0863 4.6 μMChemDiv (Drug-like library)17381640.173333
0.0552.22E-5SGTC_20305175110 200.0 μMChembridge (Fragment library)28319860.115942Golgi
0.0552.41E-5SGTC_23909097792 176.6 μMChembridge (Fragment library)44971490.102564
0.0543.00E-5SGTC_21525568786 200.0 μMChembridge (Fragment library)53998830.0533333

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1813st0512468.6 μM0.4905665291959TimTec (Natural product derivative library)253.252743.59603TSC3-RPN4
SGTC_1967st07017751.4 μM0.4347835729214TimTec (Natural product derivative library)389.400665.14705
SGTC_19284'-methoxychalcone40.3 μM0.392857641818TimTec (Natural product derivative library)238.281163.68502
SGTC_24984'-Methoxychalcone54.63 μM0.392857641818Microsource (Natural product library)238.281163.68502RPP1 & pyrimidine depletion
SGTC_1608st00147340.5 μM0.3888897292267TimTec (Natural product derivative library)242.700244.36601
SGTC_1737st03555769.7 μM0.3833335716916TimTec (Natural product derivative library)286.75284.69802
SGTC_8360330-01041.35 μM0.36842146494820ChemDiv (Drug-like library)294.518543.6103
SGTC_1706st0313219.97 μM0.3606566103185TimTec (Natural product derivative library)292.159944.28412TSC3-RPN4
SGTC_1240136-02177.12 μM0.3571433976905ChemDiv (Drug-like library)476.223245.16417mitochondrial stress
SGTC_1976st07060963.5 μM0.352941682556TimTec (Natural product derivative library)314.719844.44204