st055369

8-methyl-2-phenylchromen-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1838
Screen concentration 84.5 μM
Source TimTec (Natural product derivative library)
PubChem CID 676036
SMILES CC1=CC=CC2=C1OC(=CC2=O)C3=CC=CC=C3
Standardized SMILES Cc1cccc2C(=O)C=C(Oc12)c3ccccc3
Molecular weight 236.2653
ALogP 3.62
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.94
% growth inhibition (Hom. pool) 10.47


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 676036
Download HIP data (tab-delimited text)  (excel)
Gene:CMD1(YBR109C)|FD-Score:-3.27|P-value:5.46E-4|Clearance:0||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:ENP2(YGR145W)|FD-Score:4.15|P-value:1.67E-5|Clearance:0.13||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:ERO1(YML130C)|FD-Score:-3.24|P-value:5.90E-4|Clearance:0||SGD DESC:Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds Gene:GPI13(YLL031C)|FD-Score:3.8|P-value:7.14E-5|Clearance:0.11||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:LUC7(YDL087C)|FD-Score:4|P-value:3.13E-5|Clearance:0.2||SGD DESC:Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA Gene:MCM6(YGL201C)|FD-Score:3.28|P-value:5.27E-4|Clearance:0.21||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:MCM7(YBR202W)|FD-Score:5.35|P-value:4.45E-8|Clearance:0.98||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NUP57(YGR119C)|FD-Score:5.26|P-value:7.15E-8|Clearance:0.98||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PGI1(YBR196C)|FD-Score:4.02|P-value:2.90E-5|Clearance:0.02||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:ROT1(YMR200W)|FD-Score:-3.25|P-value:5.85E-4|Clearance:0||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPC53(YDL150W)|FD-Score:6.03|P-value:8.26E-10|Clearance:0.98||SGD DESC:RNA polymerase III subunit C53 Gene:RSP5(YER125W)|FD-Score:-3.1|P-value:9.66E-4|Clearance:0||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:SEC1(YDR164C)|FD-Score:-3.23|P-value:6.20E-4|Clearance:0||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SEC7(YDR170C)|FD-Score:4.28|P-value:9.14E-6|Clearance:0.14||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SPT6(YGR116W)|FD-Score:-3.13|P-value:8.82E-4|Clearance:0||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SSY5(YJL156C)|FD-Score:-3.1|P-value:9.56E-4|Clearance:0||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:YDL163W(YDL163W_d)|FD-Score:-3.17|P-value:7.51E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YGR114C(YGR114C_d)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YKL036C(YKL036C_d)|FD-Score:3.44|P-value:2.90E-4|Clearance:0.17||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W Gene:CMD1(YBR109C)|FD-Score:-3.27|P-value:5.46E-4|Clearance:0||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:ENP2(YGR145W)|FD-Score:4.15|P-value:1.67E-5|Clearance:0.13||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:ERO1(YML130C)|FD-Score:-3.24|P-value:5.90E-4|Clearance:0||SGD DESC:Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds Gene:GPI13(YLL031C)|FD-Score:3.8|P-value:7.14E-5|Clearance:0.11||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:LUC7(YDL087C)|FD-Score:4|P-value:3.13E-5|Clearance:0.2||SGD DESC:Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA Gene:MCM6(YGL201C)|FD-Score:3.28|P-value:5.27E-4|Clearance:0.21||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:MCM7(YBR202W)|FD-Score:5.35|P-value:4.45E-8|Clearance:0.98||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NUP57(YGR119C)|FD-Score:5.26|P-value:7.15E-8|Clearance:0.98||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PGI1(YBR196C)|FD-Score:4.02|P-value:2.90E-5|Clearance:0.02||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:ROT1(YMR200W)|FD-Score:-3.25|P-value:5.85E-4|Clearance:0||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPC53(YDL150W)|FD-Score:6.03|P-value:8.26E-10|Clearance:0.98||SGD DESC:RNA polymerase III subunit C53 Gene:RSP5(YER125W)|FD-Score:-3.1|P-value:9.66E-4|Clearance:0||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:SEC1(YDR164C)|FD-Score:-3.23|P-value:6.20E-4|Clearance:0||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SEC7(YDR170C)|FD-Score:4.28|P-value:9.14E-6|Clearance:0.14||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SPT6(YGR116W)|FD-Score:-3.13|P-value:8.82E-4|Clearance:0||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SSY5(YJL156C)|FD-Score:-3.1|P-value:9.56E-4|Clearance:0||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:YDL163W(YDL163W_d)|FD-Score:-3.17|P-value:7.51E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YGR114C(YGR114C_d)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YKL036C(YKL036C_d)|FD-Score:3.44|P-value:2.90E-4|Clearance:0.17||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 676036
Download HOP data (tab-delimited text)  (excel)
Gene:ADK1(YDR226W)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:APN1(YKL114C)|FD-Score:3.8|P-value:7.32E-5||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ART5(YGR068C)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane Gene:BFA1(YJR053W)|FD-Score:-5.1|P-value:1.71E-7||SGD DESC:Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis Gene:CLC1(YGR167W)|FD-Score:4.31|P-value:8.10E-6||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG6(YNL041C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:5.18|P-value:1.08E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.95|P-value:3.87E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUE1(YMR264W)|FD-Score:3.81|P-value:7.07E-5||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DAL81(YIR023W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBF20(YPR111W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs Gene:DPB3(YBR278W)|FD-Score:5.19|P-value:1.07E-7||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:ESC8(YOL017W)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Protein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication Gene:FES1(YBR101C)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FPR4(YLR449W)|FD-Score:4.49|P-value:3.62E-6||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:GAL11(YOL051W)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GRX6(YDL010W)|FD-Score:-6.85|P-value:3.82E-12||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:HMG1(YML075C)|FD-Score:-4.64|P-value:1.77E-6||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HNT2(YDR305C)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:HSP31(YDR533C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:MRPL4(YLR439W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:NAB6(YML117W)|FD-Score:-4.05|P-value:2.55E-5||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NFI1(YOR156C)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:PRO2(YOR323C)|FD-Score:-3.75|P-value:8.74E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:QRI7(YDL104C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification Gene:RAD54(YGL163C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:ROM2(YLR371W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPS24A(YER074W)|FD-Score:-6.67|P-value:1.24E-11||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RSC1(YGR056W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SBE22(YHR103W)|FD-Score:-6.07|P-value:6.43E-10||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SET4(YJL105W)|FD-Score:6.39|P-value:8.55E-11||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SHC1(YER096W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SNF2(YOR290C)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SOH1(YGL127C)|FD-Score:4.35|P-value:6.81E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SRB2(YHR041C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:TOP1(YOL006C)|FD-Score:5.99|P-value:1.07E-9||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TRP2(YER090W)|FD-Score:5.48|P-value:2.13E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:9.56|P-value:5.81E-22||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.86|P-value:3.35E-12||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS85(YDR108W)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBC7(YMR022W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:VEL1(YGL258W)|FD-Score:4.44|P-value:4.45E-6||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS1(YKR001C)|FD-Score:3.8|P-value:7.30E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS29(YHR012W)|FD-Score:-4.02|P-value:2.89E-5||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:YAP1802(YGR241C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YAR029W(YAR029W_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBL094C(YBL094C_d)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Gene:YCK3(YER123W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR087C-A(YCR087C-A_p)|FD-Score:4.39|P-value:5.78E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:4.38|P-value:5.99E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR169C-A(YDR169C-A_p)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YER085C(YER085C_p)|FD-Score:-3.16|P-value:7.87E-4||SGD DESC:Putative protein of unknown function Gene:YIL077C(YIL077C_p)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YLR297W(YLR297W_p)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication Gene:YMR172C-A(YMR172C-A_d)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR325W(YOR325W_d)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:YPT6(YLR262C)|FD-Score:5.96|P-value:1.27E-9||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ADK1(YDR226W)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:APN1(YKL114C)|FD-Score:3.8|P-value:7.32E-5||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ART5(YGR068C)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane Gene:BFA1(YJR053W)|FD-Score:-5.1|P-value:1.71E-7||SGD DESC:Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis Gene:CLC1(YGR167W)|FD-Score:4.31|P-value:8.10E-6||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG6(YNL041C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:5.18|P-value:1.08E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.95|P-value:3.87E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUE1(YMR264W)|FD-Score:3.81|P-value:7.07E-5||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DAL81(YIR023W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBF20(YPR111W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs Gene:DPB3(YBR278W)|FD-Score:5.19|P-value:1.07E-7||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:ESC8(YOL017W)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Protein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication Gene:FES1(YBR101C)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FPR4(YLR449W)|FD-Score:4.49|P-value:3.62E-6||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:GAL11(YOL051W)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GRX6(YDL010W)|FD-Score:-6.85|P-value:3.82E-12||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:HMG1(YML075C)|FD-Score:-4.64|P-value:1.77E-6||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HNT2(YDR305C)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:HSP31(YDR533C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:MRPL4(YLR439W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:NAB6(YML117W)|FD-Score:-4.05|P-value:2.55E-5||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NFI1(YOR156C)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:PRO2(YOR323C)|FD-Score:-3.75|P-value:8.74E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:QRI7(YDL104C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification Gene:RAD54(YGL163C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:ROM2(YLR371W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPS24A(YER074W)|FD-Score:-6.67|P-value:1.24E-11||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RSC1(YGR056W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SBE22(YHR103W)|FD-Score:-6.07|P-value:6.43E-10||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SET4(YJL105W)|FD-Score:6.39|P-value:8.55E-11||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SHC1(YER096W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SNF2(YOR290C)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SOH1(YGL127C)|FD-Score:4.35|P-value:6.81E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SRB2(YHR041C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:TOP1(YOL006C)|FD-Score:5.99|P-value:1.07E-9||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TRP2(YER090W)|FD-Score:5.48|P-value:2.13E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:9.56|P-value:5.81E-22||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.86|P-value:3.35E-12||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS85(YDR108W)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBC7(YMR022W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:VEL1(YGL258W)|FD-Score:4.44|P-value:4.45E-6||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS1(YKR001C)|FD-Score:3.8|P-value:7.30E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS29(YHR012W)|FD-Score:-4.02|P-value:2.89E-5||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:YAP1802(YGR241C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YAR029W(YAR029W_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBL094C(YBL094C_d)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Gene:YCK3(YER123W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR087C-A(YCR087C-A_p)|FD-Score:4.39|P-value:5.78E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:4.38|P-value:5.99E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR169C-A(YDR169C-A_p)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YER085C(YER085C_p)|FD-Score:-3.16|P-value:7.87E-4||SGD DESC:Putative protein of unknown function Gene:YIL077C(YIL077C_p)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YLR297W(YLR297W_p)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication Gene:YMR172C-A(YMR172C-A_d)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR325W(YOR325W_d)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:YPT6(YLR262C)|FD-Score:5.96|P-value:1.27E-9||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL150W6.038.26E-100.98RPC53RNA polymerase III subunit C53
YBR202W5.354.45E-80.98MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YGR119C5.267.15E-80.98NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YDR170C4.289.14E-60.14SEC7Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles
YGR145W4.151.67E-50.13ENP2Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p
YBR196C4.022.90E-50.02PGI1Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
YDL087C4.003.13E-50.20LUC7Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA
YLL031C3.807.14E-50.11GPI13ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein
YGR114C_d3.691.11E-40.25YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YKL036C_d3.442.90E-40.17YKL036C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W
YGL201C3.285.27E-40.21MCM6Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p
YLR208W3.060.001100.08SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YDL195W2.980.001430.00SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YDR013W2.980.001450.07PSF1Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YOR224C2.910.001790.04RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL211C9.565.81E-22TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR354W6.863.35E-12TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YJL105W6.398.55E-11SET4Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication
YOL006C5.991.07E-9TOP1Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination
YLR262C5.961.27E-9YPT6Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6
YER090W5.482.13E-8TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YBR278W5.191.07E-7DPB3Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication
YGL005C5.181.08E-7COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YLR449W4.493.62E-6FPR4Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones
YGL258W4.444.45E-6VEL1Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C
YCR087C-A_p4.395.78E-6YCR087C-A_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene
YDR008C_d4.385.99E-6YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YGL127C4.356.81E-6SOH1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits
YGR167W4.318.10E-6CLC1Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W
YDR226W4.102.07E-5ADK1Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_1838
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2636.63E-94SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.314815
0.2441.71E-80SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0392157
0.2368.82E-76SGTC_1671st016596 74.5 μMTimTec (Natural product derivative library)28291670.0857143
0.2331.62E-73SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.0909091
0.2283.18E-70SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)3015189NA
0.2257.29E-69SGTC_2741miltefosine 3.0 μMMiscellaneous35990.0350877
0.2249.06E-68SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.452381
0.2224.26E-67SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.125
0.2221.97E-66SGTC_2231micatex 6.9 μMMiscellaneous207370.0869565
0.2213.89E-66SGTC_15465-methoxyflavanone 78.7 μMTimTec (Pure natural product library)1477950.196429
0.2193.54E-65SGTC_11931488-0126 41.5 μMChemDiv (Drug-like library)13847100.0606061
0.2151.64E-62SGTC_9332914-0560 114.0 μMChemDiv (Drug-like library)4638870.0701754
0.2131.10E-61SGTC_2683piperine 63.0 μMTimTec (Pure natural product library)6380240.0769231
0.2111.46E-60SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.25
0.2112.48E-60SGTC_22037227154 166.4 μMChembridge (Fragment library)8963040.111111

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1924st05845975.1 μM0.717949688683TimTec (Natural product derivative library)266.291263.60603
SGTC_25787,8-dihydroxyflavone64.44 μM0.5751880Microsource (Natural product library)254.23752.65224
SGTC_15526-methylflavone10.6 μM0.536585689013TimTec (Pure natural product library)236.265283.62202superoxide
SGTC_24977-hydroxyflavone100 μM0.4651165281894Microsource (Natural product library)238.23812.89413
SGTC_1857st05600779.3 μM0.456522619834TimTec (Natural product derivative library)252.264683.1203
SGTC_14204'-methoxyflavone42 μM0.45454577793ChemDiv (Drug-like library)252.264683.1203
SGTC_26344'-methoxyflavone100 μM0.45454577793Microsource (Natural product library)252.264683.1203
SGTC_1975st07096730.8 μM0.452381261391TimTec (Natural product derivative library)240.2291633.34103
SGTC_1959st07709155.8 μM0.446809821356TimTec (Natural product derivative library)314.289462.61926RPP1 & pyrimidine depletion
SGTC_15615-methoxyflavone52.9 μM0.44444494525TimTec (Pure natural product library)252.264683.1203
SGTC_1955wogonin32.4 μM0.43755281703TimTec (Natural product derivative library)284.263482.63625