st057054

N-(3-fluorophenyl)-3-(2-oxochromen-3-yl)benzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1856
Screen concentration 55.7 μM
Source TimTec (Natural product derivative library)
PubChem CID 1266018
SMILES C1=CC=C2C(=C1)C=C(C(=O)O2)C3=CC(=CC=C3)C(=O)NC4=CC(=CC=C4)F
Standardized SMILES Fc1cccc(NC(=O)c2cccc(c2)C3=Cc4ccccc4OC3=O)c1
Molecular weight 359.3499
ALogP 4.37
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.18
% growth inhibition (Hom. pool) 1.24


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1266018
Download HIP data (tab-delimited text)  (excel)
Gene:ARP2(YDL029W)|FD-Score:3.66|P-value:1.28E-4|Clearance:0.5||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CWC2(YDL209C)|FD-Score:-3.96|P-value:3.77E-5|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:ERG8(YMR220W)|FD-Score:-3.11|P-value:9.39E-4|Clearance:0||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:MCD4(YKL165C)|FD-Score:3.8|P-value:7.32E-5|Clearance:0.14||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:RPL33A(YPL143W)|FD-Score:-3.37|P-value:3.78E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPO26(YPR187W)|FD-Score:5.08|P-value:1.92E-7|Clearance:1.12||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPS31(YLR167W)|FD-Score:3.16|P-value:7.91E-4|Clearance:0.02||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RPS5(YJR123W)|FD-Score:3.96|P-value:3.83E-5|Clearance:0.16||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SEN54(YPL083C)|FD-Score:-4.84|P-value:6.36E-7|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SUB2(YDL084W)|FD-Score:3.13|P-value:8.61E-4|Clearance:0.07||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UTP15(YMR093W)|FD-Score:-3.19|P-value:7.00E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YPL142C(YPL142C_d)|FD-Score:-3.25|P-value:5.77E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:ARP2(YDL029W)|FD-Score:3.66|P-value:1.28E-4|Clearance:0.5||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CWC2(YDL209C)|FD-Score:-3.96|P-value:3.77E-5|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:ERG8(YMR220W)|FD-Score:-3.11|P-value:9.39E-4|Clearance:0||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:MCD4(YKL165C)|FD-Score:3.8|P-value:7.32E-5|Clearance:0.14||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:RPL33A(YPL143W)|FD-Score:-3.37|P-value:3.78E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPO26(YPR187W)|FD-Score:5.08|P-value:1.92E-7|Clearance:1.12||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPS31(YLR167W)|FD-Score:3.16|P-value:7.91E-4|Clearance:0.02||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RPS5(YJR123W)|FD-Score:3.96|P-value:3.83E-5|Clearance:0.16||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SEN54(YPL083C)|FD-Score:-4.84|P-value:6.36E-7|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SUB2(YDL084W)|FD-Score:3.13|P-value:8.61E-4|Clearance:0.07||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UTP15(YMR093W)|FD-Score:-3.19|P-value:7.00E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YPL142C(YPL142C_d)|FD-Score:-3.25|P-value:5.77E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1266018
Download HOP data (tab-delimited text)  (excel)
Gene:AEP2(YMR282C)|FD-Score:6.13|P-value:4.52E-10||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM14(YGL160W)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:AIM45(YPR004C)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:AKR1(YDR264C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALG6(YOR002W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ATG16(YMR159C)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BFR1(YOR198C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BSC4(YNL269W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:CHS7(YHR142W)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CKA1(YIL035C)|FD-Score:-3.34|P-value:4.12E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CNA1(YLR433C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:CTF18(YMR078C)|FD-Score:-3.77|P-value:8.27E-5||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:DCC1(YCL016C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DSS1(YMR287C)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:EAF1(YDR359C)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:EGD2(YHR193C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:ERG4(YGL012W)|FD-Score:6.7|P-value:1.05E-11||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FYV12(YOR183W_p)|FD-Score:4.58|P-value:2.31E-6||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GLO2(YDR272W)|FD-Score:-3.94|P-value:4.14E-5||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GTR1(YML121W)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:IBA57(YJR122W)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:INP1(YMR204C)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IRC21(YMR073C)|FD-Score:5.06|P-value:2.13E-7||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IZH4(YOL101C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress Gene:KDX1(YKL161C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:LAA1(YJL207C)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LIP2(YLR239C)|FD-Score:5.5|P-value:1.90E-8||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:LRS4(YDR439W)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MRPL10(YNL284C)|FD-Score:4.84|P-value:6.63E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:MRPL35(YDR322W)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:OCA4(YCR095C_p)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OST3(YOR085W)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins Gene:OSW1(YOR255W)|FD-Score:4.4|P-value:5.35E-6||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:OTU2(YHL013C)|FD-Score:6.59|P-value:2.22E-11||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:OXA1(YER154W)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PEP8(YJL053W)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PHR1(YOR386W)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PIM1(YBL022C)|FD-Score:5.32|P-value:5.11E-8||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PMD1(YER132C)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:PTH1(YHR189W)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:PUG1(YER185W)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:PUS5(YLR165C)|FD-Score:3.84|P-value:6.17E-5||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:RCE1(YMR274C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RHO5(YNL180C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RPL13A(YDL082W)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:4.98|P-value:3.13E-7||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RTT107(YHR154W)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SDH1(YKL148C)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SEM1(YDR363W-A)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SET5(YHR207C)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SEY1(YOR165W)|FD-Score:6.98|P-value:1.46E-12||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SLI15(YBR156C)|FD-Score:6.48|P-value:4.50E-11||SGD DESC:Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly Gene:SNA2(YDR525W-A)|FD-Score:5.61|P-value:1.02E-8||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SNF7(YLR025W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPG5(YMR191W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SUR4(YLR372W)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWR1(YDR334W)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:THI12(YNL332W)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 Gene:TPN1(YGL186C)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TYW3(YGL050W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:YBR013C(YBR013C_p)|FD-Score:6.7|P-value:1.02E-11||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR072C-A(YBR072C-A_p)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR144C(YBR144C_d)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene Gene:YBR284W(YBR284W_p)|FD-Score:4.78|P-value:8.66E-7||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCP4(YCR004C)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YDR415C(YDR415C_p)|FD-Score:-3.26|P-value:5.60E-4||SGD DESC:Putative aminopeptidase Gene:YGL177W(YGL177W_d)|FD-Score:4.94|P-value:3.95E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR086W-A(YHR086W-A_p)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YKR070W(YKR070W_p)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YML122C(YML122C_d)|FD-Score:-4.39|P-value:5.60E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR178W(YMR178W_p)|FD-Score:4.29|P-value:8.81E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; YMR178W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL296W(YNL296W_d)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:YOL150C(YOL150C_d)|FD-Score:7.39|P-value:7.17E-14||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR053C(YPR053C_d)|FD-Score:-4.12|P-value:1.91E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C Gene:YPR123C(YPR123C_d)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPT31(YER031C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:ZTA1(YBR046C)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin Gene:AEP2(YMR282C)|FD-Score:6.13|P-value:4.52E-10||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM14(YGL160W)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:AIM45(YPR004C)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:AKR1(YDR264C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALG6(YOR002W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ATG16(YMR159C)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BFR1(YOR198C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BSC4(YNL269W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:CHS7(YHR142W)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CKA1(YIL035C)|FD-Score:-3.34|P-value:4.12E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CNA1(YLR433C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:CTF18(YMR078C)|FD-Score:-3.77|P-value:8.27E-5||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:DCC1(YCL016C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DSS1(YMR287C)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:EAF1(YDR359C)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:EGD2(YHR193C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:ERG4(YGL012W)|FD-Score:6.7|P-value:1.05E-11||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FYV12(YOR183W_p)|FD-Score:4.58|P-value:2.31E-6||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GLO2(YDR272W)|FD-Score:-3.94|P-value:4.14E-5||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GTR1(YML121W)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:IBA57(YJR122W)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:INP1(YMR204C)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IRC21(YMR073C)|FD-Score:5.06|P-value:2.13E-7||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IZH4(YOL101C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress Gene:KDX1(YKL161C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:LAA1(YJL207C)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LIP2(YLR239C)|FD-Score:5.5|P-value:1.90E-8||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:LRS4(YDR439W)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MRPL10(YNL284C)|FD-Score:4.84|P-value:6.63E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:MRPL35(YDR322W)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:OCA4(YCR095C_p)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OST3(YOR085W)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins Gene:OSW1(YOR255W)|FD-Score:4.4|P-value:5.35E-6||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:OTU2(YHL013C)|FD-Score:6.59|P-value:2.22E-11||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:OXA1(YER154W)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PEP8(YJL053W)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PHR1(YOR386W)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PIM1(YBL022C)|FD-Score:5.32|P-value:5.11E-8||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PMD1(YER132C)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:PTH1(YHR189W)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:PUG1(YER185W)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:PUS5(YLR165C)|FD-Score:3.84|P-value:6.17E-5||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:RCE1(YMR274C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RHO5(YNL180C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RPL13A(YDL082W)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:4.98|P-value:3.13E-7||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RTT107(YHR154W)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SDH1(YKL148C)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SEM1(YDR363W-A)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SET5(YHR207C)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SEY1(YOR165W)|FD-Score:6.98|P-value:1.46E-12||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SLI15(YBR156C)|FD-Score:6.48|P-value:4.50E-11||SGD DESC:Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly Gene:SNA2(YDR525W-A)|FD-Score:5.61|P-value:1.02E-8||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SNF7(YLR025W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPG5(YMR191W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SUR4(YLR372W)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWR1(YDR334W)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:THI12(YNL332W)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 Gene:TPN1(YGL186C)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TYW3(YGL050W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:YBR013C(YBR013C_p)|FD-Score:6.7|P-value:1.02E-11||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR072C-A(YBR072C-A_p)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR144C(YBR144C_d)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene Gene:YBR284W(YBR284W_p)|FD-Score:4.78|P-value:8.66E-7||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCP4(YCR004C)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YDR415C(YDR415C_p)|FD-Score:-3.26|P-value:5.60E-4||SGD DESC:Putative aminopeptidase Gene:YGL177W(YGL177W_d)|FD-Score:4.94|P-value:3.95E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR086W-A(YHR086W-A_p)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YKR070W(YKR070W_p)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YML122C(YML122C_d)|FD-Score:-4.39|P-value:5.60E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR178W(YMR178W_p)|FD-Score:4.29|P-value:8.81E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; YMR178W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL296W(YNL296W_d)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:YOL150C(YOL150C_d)|FD-Score:7.39|P-value:7.17E-14||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR053C(YPR053C_d)|FD-Score:-4.12|P-value:1.91E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C Gene:YPR123C(YPR123C_d)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPT31(YER031C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:ZTA1(YBR046C)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR187W5.081.92E-71.12RPO26RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
YJR123W3.963.83E-50.16RPS5Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7
YKL165C3.807.32E-50.14MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YDL029W3.661.28E-40.50ARP2Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YLR167W3.167.91E-40.02RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YDL084W3.138.61E-40.07SUB2Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress
YDR449C3.060.001100.12UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YLR378C2.950.001600.14SEC61Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER
YGL171W2.800.002530.04ROK1RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation
YBL004W2.770.002820.03UTP20Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
YGR147C2.740.003060.02NAT2Protein with an apparent role in acetylation of N-terminal methionine residues
YMR268C2.720.003240.00PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YOL144W2.720.003280.06NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YHR188C2.660.003910.03GPI16Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog
YAL041W2.630.004310.02CDC24Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOL150C_d7.397.17E-14YOL150C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR165W6.981.46E-12SEY1Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3
YBR013C_p6.701.02E-11YBR013C_pPutative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein
YGL012W6.701.05E-11ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YHL013C6.592.22E-11OTU2Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress
YBR156C6.484.50E-11SLI15Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly
YMR282C6.134.52E-10AEP2Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader
YDR525W-A5.611.02E-8SNA2Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YLR239C5.501.90E-8LIP2Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
YBL022C5.325.11E-8PIM1ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
YMR073C5.062.13E-7IRC21Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study
YMR194W4.983.13E-7RPL36ARibosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication
YGL177W_d4.943.95E-7YGL177W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YNL284C4.846.63E-7MRPL10Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels
YBR284W_p4.788.66E-7YBR284W_pPutative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1856
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0881.46E-11SGTC_1852st056254 31.4 μMTimTec (Natural product derivative library)4425830.2
0.0731.71E-8SGTC_2555cholic acid 100.0 μMMicrosource (Natural product library)67086570.0306122
0.0722.77E-8SGTC_7083448-5400 146.0 μMChemDiv (Drug-like library)34621950.11
0.0722.84E-8SGTC_8470342-0274 5.5 μMChemDiv (Drug-like library)180970.106061
0.0715.73E-8SGTC_13772944-0135 198.0 μMChemDiv (Drug-like library)18941370.091954calcium & mitochondrial duress
0.0681.83E-7SGTC_29779011197 71.4 μMChembridge (Drug-like library)27047220.327869
0.0672.15E-7SGTC_797k781-0471 270.0 μMChemDiv (Drug-like library)237381290.115385RPP1 & pyrimidine depletion
0.0631.19E-6SGTC_6260214-0009 33.0 μMChemDiv (Drug-like library)34378740.242424copper-dependent oxidative stress
0.0631.36E-6SGTC_1812st052057 4.3 μMTimTec (Natural product derivative library)36454180.0306122calcium & mitochondrial duress
0.0612.88E-6SGTC_29459067681 61.8 μMChembridge (Drug-like library)164569300.120482
0.0612.88E-6SGTC_2526patulin 37.6 μMMicrosource (Natural product library)46960.104478
0.0595.61E-6SGTC_1130099-0221 2.5 μMChemDiv (Drug-like library)892190.0595238
0.0587.12E-6SGTC_117lucigenin 22.1 μMChemDiv (Drug-like library)651000.0833333redox potentiating
0.0571.14E-5SGTC_1758st042942 75.2 μMTimTec (Natural product derivative library)6693170.161765
0.0552.21E-5SGTC_1624st003710 6.2 μMTimTec (Natural product derivative library)242070330.0792079calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_13671812-051076.9 μM0.6101691142582ChemDiv (Drug-like library)410.217584.12614
SGTC_1655st01254655.1 μM0.509434720985TimTec (Natural product derivative library)237.253342.63113
SGTC_1703st02698348.3 μM0.4782611104023TimTec (Natural product derivative library)414.410125.02915
SGTC_9041141-0088396 μM0.476191378119ChemDiv (Drug-like library)371.385384.14614
SGTC_2889905965651.95 μM0.3859653949838Chembridge (Drug-like library)287.1848563.64216
SGTC_8810833-0569170 μM0.3809525418700ChemDiv (Drug-like library)306.315362.68414
SGTC_2883905914545.46 μM0.38095216476510Chembridge (Drug-like library)269.2703833.99613
SGTC_1870st05751426.5 μM0.3770495393161TimTec (Natural product derivative library)317.134163.88413RSC & ERG11
SGTC_1964st07691461 μM0.37313424208661TimTec (Natural product derivative library)327.761663.82714RPP1 & pyrimidine depletion
SGTC_1628st00425277.7 μM0.354839692968TimTec (Natural product derivative library)257.28452.4713