st056228

6-hydroxy-2-(4-methoxyphenyl)chromen-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1860
Screen concentration 74.6 μM
Source TimTec (Natural product derivative library)
PubChem CID 688679
SMILES COC1=CC=C(C=C1)C2=CC(=O)C3=C(O2)C=CC(=C3)O
Standardized SMILES COc1ccc(cc1)C2=CC(=O)c3cc(O)ccc3O2
Molecular weight 268.2641
ALogP 2.88
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.99
% growth inhibition (Hom. pool) -1.03


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 688679
Download HIP data (tab-delimited text)  (excel)
Gene:ARC15(YIL062C)|FD-Score:3.85|P-value:5.82E-5|Clearance:0.11||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:BET3(YKR068C)|FD-Score:4.29|P-value:9.05E-6|Clearance:0.02||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:CCT8(YJL008C)|FD-Score:4.86|P-value:5.81E-7|Clearance:0.57||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC6(YJL194W)|FD-Score:-3.46|P-value:2.71E-4|Clearance:0||SGD DESC:Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:COG2(YGR120C)|FD-Score:-7.48|P-value:3.64E-14|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DNA2(YHR164C)|FD-Score:-3.87|P-value:5.40E-5|Clearance:0||SGD DESC:Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress Gene:HRP1(YOL123W)|FD-Score:3.61|P-value:1.56E-4|Clearance:0.17||SGD DESC:Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences Gene:MEC1(YBR136W)|FD-Score:3.41|P-value:3.30E-4|Clearance:0.06||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:MPS3(YJL019W)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.08||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:NOP4(YPL043W)|FD-Score:-3.7|P-value:1.08E-4|Clearance:0||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:NOP58(YOR310C)|FD-Score:-3.65|P-value:1.32E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NSE5(YML023C)|FD-Score:-3.18|P-value:7.43E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:NSP1(YJL041W)|FD-Score:3.74|P-value:9.12E-5|Clearance:0.14||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:PRP38(YGR075C)|FD-Score:4.27|P-value:9.94E-6|Clearance:0.06||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:PWP2(YCR057C)|FD-Score:4.21|P-value:1.30E-5|Clearance:0.35||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPL5(YPL131W)|FD-Score:3.44|P-value:2.96E-4|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPP1(YHR062C)|FD-Score:-7.09|P-value:6.47E-13|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS5(YJR123W)|FD-Score:7.67|P-value:8.44E-15|Clearance:2.81||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SPN1(YPR133C)|FD-Score:3.26|P-value:5.64E-4|Clearance:0.16||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TAF12(YDR145W)|FD-Score:-3.61|P-value:1.50E-4|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:YJR012C(YJR012C_p)|FD-Score:3.09|P-value:9.93E-4|Clearance:0.01||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W Gene:ARC15(YIL062C)|FD-Score:3.85|P-value:5.82E-5|Clearance:0.11||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:BET3(YKR068C)|FD-Score:4.29|P-value:9.05E-6|Clearance:0.02||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:CCT8(YJL008C)|FD-Score:4.86|P-value:5.81E-7|Clearance:0.57||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC6(YJL194W)|FD-Score:-3.46|P-value:2.71E-4|Clearance:0||SGD DESC:Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:COG2(YGR120C)|FD-Score:-7.48|P-value:3.64E-14|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DNA2(YHR164C)|FD-Score:-3.87|P-value:5.40E-5|Clearance:0||SGD DESC:Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress Gene:HRP1(YOL123W)|FD-Score:3.61|P-value:1.56E-4|Clearance:0.17||SGD DESC:Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences Gene:MEC1(YBR136W)|FD-Score:3.41|P-value:3.30E-4|Clearance:0.06||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:MPS3(YJL019W)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.08||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:NOP4(YPL043W)|FD-Score:-3.7|P-value:1.08E-4|Clearance:0||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:NOP58(YOR310C)|FD-Score:-3.65|P-value:1.32E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NSE5(YML023C)|FD-Score:-3.18|P-value:7.43E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:NSP1(YJL041W)|FD-Score:3.74|P-value:9.12E-5|Clearance:0.14||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:PRP38(YGR075C)|FD-Score:4.27|P-value:9.94E-6|Clearance:0.06||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:PWP2(YCR057C)|FD-Score:4.21|P-value:1.30E-5|Clearance:0.35||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPL5(YPL131W)|FD-Score:3.44|P-value:2.96E-4|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPP1(YHR062C)|FD-Score:-7.09|P-value:6.47E-13|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS5(YJR123W)|FD-Score:7.67|P-value:8.44E-15|Clearance:2.81||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SPN1(YPR133C)|FD-Score:3.26|P-value:5.64E-4|Clearance:0.16||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TAF12(YDR145W)|FD-Score:-3.61|P-value:1.50E-4|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:YJR012C(YJR012C_p)|FD-Score:3.09|P-value:9.93E-4|Clearance:0.01||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 688679
Download HOP data (tab-delimited text)  (excel)
Gene:ACO1(YLR304C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ATG18(YFR021W)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP14(YLR295C)|FD-Score:3.88|P-value:5.12E-5||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BEM4(YPL161C)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:CAM1(YPL048W)|FD-Score:4.38|P-value:5.95E-6||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CIN8(YEL061C)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CSH1(YBR161W)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:CUE1(YMR264W)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DIP5(YPL265W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress Gene:DOT6(YER088C)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ECI1(YLR284C)|FD-Score:-4.43|P-value:4.71E-6||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:ECM32(YER176W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:FAR3(YMR052W)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:GPA2(YER020W)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GPX1(YKL026C)|FD-Score:4.28|P-value:9.21E-6||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress Gene:GTB1(YDR221W)|FD-Score:4.9|P-value:4.73E-7||SGD DESC:Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress Gene:HIS7(YBR248C)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor Gene:HXT8(YJL214W)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:HYR1(YIR037W)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:IDH2(YOR136W)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:LEM3(YNL323W)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MDM30(YLR368W)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains Gene:MED1(YPR070W)|FD-Score:3.76|P-value:8.47E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MET1(YKR069W)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MRM1(YOR201C)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:OMA1(YKR087C)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:OTU2(YHL013C)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PAC10(YGR078C)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PHR1(YOR386W)|FD-Score:4.29|P-value:9.02E-6||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PRS5(YOL061W)|FD-Score:-4.83|P-value:6.78E-7||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress Gene:RBL2(YOR265W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress Gene:REF2(YDR195W)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RNH1(YMR234W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:ROT2(YBR229C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPL8A(YHL033C)|FD-Score:5.26|P-value:7.03E-8||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:4.3|P-value:8.68E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RTN1(YDR233C)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication Gene:SAS3(YBL052C)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SBH1(YER087C-B)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SLM4(YBR077C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SNF6(YHL025W)|FD-Score:-3.12|P-value:8.89E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SRY1(YKL218C)|FD-Score:-5.32|P-value:5.33E-8||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:TDA11(YHR159W_p)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:THI21(YPL258C)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p Gene:TRP1(YDR007W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UME6(YDR207C)|FD-Score:3.78|P-value:8.00E-5||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:URA10(YMR271C)|FD-Score:5.27|P-value:6.79E-8||SGD DESC:Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication Gene:VBA2(YBR293W)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VNX1(YNL321W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:VPS69(YPR087W_d)|FD-Score:-3.37|P-value:3.83E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:WTM2(YOR229W)|FD-Score:3.84|P-value:6.14E-5||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YAP1801(YHR161C)|FD-Score:3.19|P-value:6.99E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YBR051W(YBR051W_d)|FD-Score:4.4|P-value:5.30E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YBR196C-B(YBR196C-B_p)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCR085W(YCR085W_d)|FD-Score:-3.76|P-value:8.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR100C(YCR100C_p)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Putative protein of unknown function Gene:YEL057C(YEL057C_p)|FD-Score:3.74|P-value:9.37E-5||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YEN1(YER041W)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YFR032C-B(YFR032C-B_p)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YJL132W(YJL132W_p)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene Gene:YLL059C(YLL059C_d)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML054C-A(YML054C-A_p)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Putative protein of unknown function Gene:YMR226C(YMR226C)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments Gene:YOL013W-A(YOL013W-A_p)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YOL164W-A(YOL164W-A_p)|FD-Score:-7.22|P-value:2.63E-13||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR014C(YPR014C_d)|FD-Score:8.48|P-value:1.10E-17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR117W(YPR117W_p)|FD-Score:3.87|P-value:5.39E-5||SGD DESC:Putative protein of unknown function Gene:ACO1(YLR304C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ATG18(YFR021W)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP14(YLR295C)|FD-Score:3.88|P-value:5.12E-5||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BEM4(YPL161C)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:CAM1(YPL048W)|FD-Score:4.38|P-value:5.95E-6||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CIN8(YEL061C)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CSH1(YBR161W)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:CUE1(YMR264W)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DIP5(YPL265W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress Gene:DOT6(YER088C)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ECI1(YLR284C)|FD-Score:-4.43|P-value:4.71E-6||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:ECM32(YER176W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:FAR3(YMR052W)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:GPA2(YER020W)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GPX1(YKL026C)|FD-Score:4.28|P-value:9.21E-6||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress Gene:GTB1(YDR221W)|FD-Score:4.9|P-value:4.73E-7||SGD DESC:Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress Gene:HIS7(YBR248C)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor Gene:HXT8(YJL214W)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:HYR1(YIR037W)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:IDH2(YOR136W)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:LEM3(YNL323W)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MDM30(YLR368W)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains Gene:MED1(YPR070W)|FD-Score:3.76|P-value:8.47E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MET1(YKR069W)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MRM1(YOR201C)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:OMA1(YKR087C)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:OTU2(YHL013C)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PAC10(YGR078C)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PHR1(YOR386W)|FD-Score:4.29|P-value:9.02E-6||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PRS5(YOL061W)|FD-Score:-4.83|P-value:6.78E-7||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress Gene:RBL2(YOR265W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress Gene:REF2(YDR195W)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RNH1(YMR234W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:ROT2(YBR229C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPL8A(YHL033C)|FD-Score:5.26|P-value:7.03E-8||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:4.3|P-value:8.68E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RTN1(YDR233C)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication Gene:SAS3(YBL052C)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SBH1(YER087C-B)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SLM4(YBR077C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SNF6(YHL025W)|FD-Score:-3.12|P-value:8.89E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SRY1(YKL218C)|FD-Score:-5.32|P-value:5.33E-8||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:TDA11(YHR159W_p)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:THI21(YPL258C)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p Gene:TRP1(YDR007W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UME6(YDR207C)|FD-Score:3.78|P-value:8.00E-5||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:URA10(YMR271C)|FD-Score:5.27|P-value:6.79E-8||SGD DESC:Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication Gene:VBA2(YBR293W)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VNX1(YNL321W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:VPS69(YPR087W_d)|FD-Score:-3.37|P-value:3.83E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:WTM2(YOR229W)|FD-Score:3.84|P-value:6.14E-5||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YAP1801(YHR161C)|FD-Score:3.19|P-value:6.99E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YBR051W(YBR051W_d)|FD-Score:4.4|P-value:5.30E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YBR196C-B(YBR196C-B_p)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCR085W(YCR085W_d)|FD-Score:-3.76|P-value:8.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR100C(YCR100C_p)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Putative protein of unknown function Gene:YEL057C(YEL057C_p)|FD-Score:3.74|P-value:9.37E-5||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YEN1(YER041W)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YFR032C-B(YFR032C-B_p)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YJL132W(YJL132W_p)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene Gene:YLL059C(YLL059C_d)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML054C-A(YML054C-A_p)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Putative protein of unknown function Gene:YMR226C(YMR226C)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments Gene:YOL013W-A(YOL013W-A_p)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YOL164W-A(YOL164W-A_p)|FD-Score:-7.22|P-value:2.63E-13||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR014C(YPR014C_d)|FD-Score:8.48|P-value:1.10E-17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR117W(YPR117W_p)|FD-Score:3.87|P-value:5.39E-5||SGD DESC:Putative protein of unknown function

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJR123W7.678.44E-152.81RPS5Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7
YJL008C4.865.81E-70.57CCT8Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YKR068C4.299.05E-60.02BET3Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex
YGR075C4.279.94E-60.06PRP38Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly
YCR057C4.211.30E-50.35PWP2Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis
YIL062C3.855.82E-50.11ARC15Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity
YJL041W3.749.12E-50.14NSP1FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress
YOL123W3.611.56E-40.17HRP1Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences
YPL131W3.442.96E-40.03RPL5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
YBR136W3.413.30E-40.06MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YJL019W3.344.17E-40.08MPS3Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
YPR133C3.265.64E-40.16SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YJR012C_p3.099.93E-40.01YJR012C_pEssential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W
YPL122C3.080.001040.02TFB2Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH
YLR076C_d3.060.001100.00YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPR014C_d8.481.10E-17YPR014C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene
YMR271C5.276.79E-8URA10Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication
YHL033C5.267.03E-8RPL8ARibosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication
YDR221W4.904.73E-7GTB1Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress
YNL323W4.651.66E-6LEM3Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane
YHR159W_p4.641.72E-6TDA11_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele
YBR051W_d4.405.30E-6YBR051W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase
YPL048W4.385.95E-6CAM1Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma
YER074W4.308.68E-6RPS24AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication
YOR386W4.299.02E-6PHR1DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p
YKL026C4.289.21E-6GPX1Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress
YLR304C4.211.27E-5ACO1Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy
YMR052W4.201.34E-5FAR3Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
YFR021W4.201.34E-5ATG18Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood
YNL321W4.161.61E-5VNX1Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate

GO enrichment analysis for SGTC_1860
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0655.92E-7SGTC_3252810-4341 181.0 μMChemDiv (Drug-like library)7749700.119403amide catabolism
0.0589.14E-6SGTC_5961155-0152 116.0 μMChemDiv (Drug-like library)2803130.101695TRP & mitochondrial translation
0.0589.40E-6SGTC_22907904846 200.0 μMChembridge (Fragment library)29621540.0909091
0.0589.70E-6SGTC_74000s-0487 160.3 μMChemDiv (Drug-like library)6775480.107692
0.0571.36E-5SGTC_7371950-0117 57.9 μMChemDiv (Drug-like library)53935130.0519481
0.0561.52E-5SGTC_1559coumarin 343 70.1 μMTimTec (Pure natural product library)1087700.128571
0.0561.83E-5SGTC_1901st060216 58.6 μMTimTec (Natural product derivative library)6887920.313726
0.0534.08E-5SGTC_32019113999 49.5 μMChembridge (Drug-like library)166492240.111111
0.0534.24E-5SGTC_287k060-0039 29.6 μMChemDiv (Drug-like library)58619740.0547945
0.0534.44E-5SGTC_31589100007 49.5 μMChembridge (Drug-like library)192972330.084507
0.0517.79E-5SGTC_305-(2-thienylidene)-Rhodanine 28.7 μMChemDiv (Drug-like library)12411320.078125
0.0518.08E-5SGTC_11604092-0570 10.5 μMChemDiv (Drug-like library)49949750.109589plasma membrane duress
0.0519.36E-5SGTC_2747doxorubicin 7.6 μMMiscellaneous317030.135417anthracycline transcription coupled DNA repair
0.0501.35E-4SGTC_22386671620 73.2 μMChembridge (Fragment library)7299410.09375
0.0491.61E-4SGTC_5794491-1009 110.0 μMChemDiv (Drug-like library)12710410.075757660S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1111tnp00062510.7 nM0.85676306TimTec (Natural product library)268.264082.87814
SGTC_14204'-methoxyflavone42 μM0.66666777793ChemDiv (Drug-like library)252.264683.1203
SGTC_26344'-methoxyflavone100 μM0.66666777793Microsource (Natural product library)252.264683.1203
SGTC_18943',6'-dihydroxyflavone78.7 μM0.627907688662TimTec (Natural product derivative library)254.23752.65224
SGTC_2636apigenin dimethyl ether100 μM0.6086965281601Microsource (Natural product library)298.290062.86115
SGTC_15427-hydroxy-3'-methoxyflavone49.7 μM0.6041675393153TimTec (Pure natural product library)268.264082.87814
SGTC_1965Pectolinarigenin58.4 μM0.5714295320438TimTec (Natural product derivative library)314.289462.61926RPP1 & pyrimidine depletion
SGTC_1866st05763864 μM0.565217688802TimTec (Natural product derivative library)312.316643.08705
SGTC_1833st05598471 μM0.541667676288TimTec (Natural product derivative library)282.290663.10304
SGTC_24977-hydroxyflavone100 μM0.5333335281894Microsource (Natural product library)238.23812.89413
SGTC_15924',5'-dihydroxyflavone8.85 μM0.520833688669TimTec (Pure natural product library)282.290663.1030460S ribosome export