st057645

3-methoxy-2-(4-methoxyphenyl)chromen-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1864
Screen concentration 30.4 μM
Source TimTec (Natural product derivative library)
PubChem CID 688666
SMILES COC1=CC=C(C=C1)C2=C(C(=O)C3=CC=CC=C3O2)OC
Standardized SMILES COC1=C(Oc2ccccc2C1=O)c3ccc(OC)cc3
Molecular weight 282.2907
ALogP 2.81
H-bond donor count 0
H-bond acceptor count 4
Response signature amide catabolism

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.86
% growth inhibition (Hom. pool) 3.63


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 688666
Download HIP data (tab-delimited text)  (excel)
Gene:ARP2(YDL029W)|FD-Score:3.52|P-value:2.19E-4|Clearance:0.09||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:DAM1(YGR113W)|FD-Score:-3.09|P-value:9.99E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:GFA1(YKL104C)|FD-Score:3.1|P-value:9.73E-4|Clearance:0.01||SGD DESC:Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication Gene:KRE29(YER038C)|FD-Score:3.86|P-value:5.56E-5|Clearance:0.35||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:LCB1(YMR296C)|FD-Score:-3.79|P-value:7.52E-5|Clearance:0||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LUC7(YDL087C)|FD-Score:3.32|P-value:4.50E-4|Clearance:0.05||SGD DESC:Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA Gene:MMF1(YIL051C)|FD-Score:-3.28|P-value:5.23E-4|Clearance:0||SGD DESC:Mitochondrial protein required for transamination of isoleucine but not of valine or leucine; may regulate specificity of branched-chain transaminases Bat1p and Bat2p; interacts genetically with mitochondrial ribosomal protein genes Gene:NSE4(YDL105W)|FD-Score:3.09|P-value:9.92E-4|Clearance:0.02||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:RPC19(YNL113W)|FD-Score:4.1|P-value:2.07E-5|Clearance:0.23||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:SEC2(YNL272C)|FD-Score:3.27|P-value:5.47E-4|Clearance:0.17||SGD DESC:Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles Gene:TSC10(YBR265W)|FD-Score:3.42|P-value:3.12E-4|Clearance:0.1||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:YJL086C(YJL086C_d)|FD-Score:4.48|P-value:3.69E-6|Clearance:0.38||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:ARP2(YDL029W)|FD-Score:3.52|P-value:2.19E-4|Clearance:0.09||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:DAM1(YGR113W)|FD-Score:-3.09|P-value:9.99E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:GFA1(YKL104C)|FD-Score:3.1|P-value:9.73E-4|Clearance:0.01||SGD DESC:Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication Gene:KRE29(YER038C)|FD-Score:3.86|P-value:5.56E-5|Clearance:0.35||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:LCB1(YMR296C)|FD-Score:-3.79|P-value:7.52E-5|Clearance:0||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LUC7(YDL087C)|FD-Score:3.32|P-value:4.50E-4|Clearance:0.05||SGD DESC:Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA Gene:MMF1(YIL051C)|FD-Score:-3.28|P-value:5.23E-4|Clearance:0||SGD DESC:Mitochondrial protein required for transamination of isoleucine but not of valine or leucine; may regulate specificity of branched-chain transaminases Bat1p and Bat2p; interacts genetically with mitochondrial ribosomal protein genes Gene:NSE4(YDL105W)|FD-Score:3.09|P-value:9.92E-4|Clearance:0.02||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:RPC19(YNL113W)|FD-Score:4.1|P-value:2.07E-5|Clearance:0.23||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:SEC2(YNL272C)|FD-Score:3.27|P-value:5.47E-4|Clearance:0.17||SGD DESC:Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles Gene:TSC10(YBR265W)|FD-Score:3.42|P-value:3.12E-4|Clearance:0.1||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:YJL086C(YJL086C_d)|FD-Score:4.48|P-value:3.69E-6|Clearance:0.38||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 688666
Download HOP data (tab-delimited text)  (excel)
Gene:APL3(YBL037W)|FD-Score:-3.16|P-value:7.86E-4||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:BCH1(YMR237W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BCH2(YKR027W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BDF1(YLR399C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BEM3(YPL115C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:DAL81(YIR023W)|FD-Score:7.3|P-value:1.45E-13||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DPB4(YDR121W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization Gene:EAR1(YMR171C)|FD-Score:3.85|P-value:5.93E-5||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:EMC6(YLL014W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:HFI1(YPL254W)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HIS3(YOR202W)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p Gene:JNM1(YMR294W)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LIP5(YOR196C)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:MET12(YPL023C)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:PTC4(YBR125C)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:RPS18B(YML026C)|FD-Score:5.17|P-value:1.14E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS22A(YJL190C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:SNF7(YLR025W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:TRP2(YER090W)|FD-Score:4.49|P-value:3.60E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UBX4(YMR067C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VOA1(YGR106C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS21(YOR089C)|FD-Score:3.74|P-value:9.31E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YCK3(YER123W)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR022C(YCR022C_d)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YCR087C-A(YCR087C-A_p)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YKR051W(YKR051W_p)|FD-Score:3.76|P-value:8.36E-5||SGD DESC:Putative protein of unknown function Gene:YLR402W(YLR402W_d)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL019W(YOL019W_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YOR072W(YOR072W_d)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPR014C(YPR014C_d)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR097W(YPR097W)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR197C(YPR197C_d)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YSR3(YKR053C)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication Gene:YUR1(YJL139C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication Gene:ZTA1(YBR046C)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin Gene:APL3(YBL037W)|FD-Score:-3.16|P-value:7.86E-4||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:BCH1(YMR237W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BCH2(YKR027W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BDF1(YLR399C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BEM3(YPL115C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:DAL81(YIR023W)|FD-Score:7.3|P-value:1.45E-13||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DPB4(YDR121W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization Gene:EAR1(YMR171C)|FD-Score:3.85|P-value:5.93E-5||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:EMC6(YLL014W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:HFI1(YPL254W)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HIS3(YOR202W)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p Gene:JNM1(YMR294W)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LIP5(YOR196C)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:MET12(YPL023C)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:PTC4(YBR125C)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:RPS18B(YML026C)|FD-Score:5.17|P-value:1.14E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS22A(YJL190C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:SNF7(YLR025W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:TRP2(YER090W)|FD-Score:4.49|P-value:3.60E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UBX4(YMR067C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VOA1(YGR106C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS21(YOR089C)|FD-Score:3.74|P-value:9.31E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YCK3(YER123W)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR022C(YCR022C_d)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YCR087C-A(YCR087C-A_p)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YKR051W(YKR051W_p)|FD-Score:3.76|P-value:8.36E-5||SGD DESC:Putative protein of unknown function Gene:YLR402W(YLR402W_d)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL019W(YOL019W_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YOR072W(YOR072W_d)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPR014C(YPR014C_d)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR097W(YPR097W)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR197C(YPR197C_d)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YSR3(YKR053C)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication Gene:YUR1(YJL139C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication Gene:ZTA1(YBR046C)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL086C_d4.483.69E-60.38YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YNL113W4.102.07E-50.23RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YER038C3.865.56E-50.35KRE29Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
YDL029W3.522.19E-40.09ARP2Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YBR265W3.423.12E-40.10TSC103-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family
YDL087C3.324.50E-40.05LUC7Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA
YNL272C3.275.47E-40.17SEC2Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles
YKL104C3.109.73E-40.01GFA1Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication
YDL105W3.099.92E-40.02NSE4Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YLR355C3.070.001070.11ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YDL028C2.960.001530.07MPS1Dual-specificity kinase; required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p
YNL150W_d2.890.001930.05YNL150W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking
YFL039C2.840.002280.02ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YJL009W_d2.820.002430.03YJL009W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex
YOR340C2.790.002650.08RPA43RNA polymerase I subunit A43

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIR023W7.301.45E-13DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YML026C5.171.14E-7RPS18BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YER090W4.493.60E-6TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YPR097W3.944.05E-5YPR097WProtein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YPL023C3.865.59E-5MET12Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR
YMR171C3.855.93E-5EAR1Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p
YKR051W_p3.768.36E-5YKR051W_pPutative protein of unknown function
YOR089C3.749.31E-5VPS21Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
YLR402W_d3.691.13E-4YLR402W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YOL019W_p3.581.70E-4YOL019W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication
YLR025W3.561.83E-4SNF7One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
YGR106C3.551.96E-4VOA1Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval
YLR399C3.532.08E-4BDF1Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p
YPR014C_d3.502.37E-4YPR014C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene
YKR027W3.482.50E-4BCH2Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1864
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2001.97E-54SGTC_486niguldipine 82.0 μMMiscellaneous12360.0989011amide catabolism
0.1959.19E-52SGTC_24605552655 174.2 μMMiscellaneous54019890.09375amide catabolism
0.1756.27E-42SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0983607
0.1583.44E-34SGTC_28459003024 43.0 μMChembridge (Drug-like library)29901730.106061amide catabolism
0.1561.17E-33SGTC_5694130-2812 160.0 μMChemDiv (Drug-like library)7435520.0847458amide catabolism
0.1531.92E-32SGTC_10563448-1962 115.0 μMChemDiv (Drug-like library)7155070.133333amide catabolism
0.1533.89E-32SGTC_3371315-0038 354.0 μMChemDiv (Drug-like library)16264370.0769231amide catabolism
0.1505.05E-31SGTC_3252810-4341 181.0 μMChemDiv (Drug-like library)7749700.107692amide catabolism
0.1441.36E-28SGTC_9903985-0011 41.3 μMChemDiv (Drug-like library)53995650.220339amide catabolism
0.1352.73E-25SGTC_24615755302 26.1 μMMiscellaneous27889040.1
0.1331.08E-24SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.111111
0.1314.67E-24SGTC_2683piperine 63.0 μMTimTec (Pure natural product library)6380240.0895522
0.1309.38E-24SGTC_32289131799 49.5 μMChembridge (Drug-like library)170158180.0985916amide catabolism
0.1257.45E-22SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.178571
0.1231.94E-21SGTC_11811222-0449 220.0 μMChemDiv (Drug-like library)6583180.133333

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1868st05764442.7 μM0.731707688665TimTec (Natural product derivative library)282.290662.80804
SGTC_15494'-methoxyflavonol74.6 μM0.72597141TimTec (Pure natural product library)268.264082.58214
SGTC_18693',7'-dimethoxyflavone40.3 μM0.707317688664TimTec (Natural product derivative library)282.290662.80804
SGTC_25623',7'-dimethoxyflavone64.92 μM0.707317688664Microsource (Natural product library)282.290662.80804
SGTC_1865st05763952.2 μM0.613636688818TimTec (Natural product derivative library)312.316642.79105
SGTC_26222-methoxyxanthone59.94 μM0.56097671034Microsource (Natural product library)226.22742.94803
SGTC_14204'-methoxyflavone42 μM0.5581477793ChemDiv (Drug-like library)252.264683.1203
SGTC_26344'-methoxyflavone100 μM0.5581477793Microsource (Natural product library)252.264683.1203
SGTC_25903,4',5',6',7'-pentamethoxyflavone100 μM0.553191521171Microsource (Natural product library)372.36862.7580760S ribosome export
SGTC_11103-hydroxy-3'-methoxyflavone1.24 μM0.531915676296TimTec (Natural product library)268.264082.58214iron homeostasis
SGTC_1961st07655068.7 μM0.5306121579337TimTec (Natural product derivative library)291.300723.04104
SGTC_1693xanthone101.9 μM0.4444447020TimTec (Natural product derivative library)196.201422.96402DNA damage response