st057638

2-(2,4-dimethoxyphenyl)-6-methoxychromen-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1866
Screen concentration 64.0 μM
Source TimTec (Natural product derivative library)
PubChem CID 688802
SMILES COC1=CC2=C(C=C1)OC(=CC2=O)C3=C(C=C(C=C3)OC)OC
Standardized SMILES COc1ccc(C2=CC(=O)c3cc(OC)ccc3O2)c(OC)c1
Molecular weight 312.3166
ALogP 3.09
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.3
% growth inhibition (Hom. pool) 1.3


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 688802
Download HIP data (tab-delimited text)  (excel)
Gene:ALG7(YBR243C)|FD-Score:-4.57|P-value:2.42E-6|Clearance:0||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:CAB2(YIL083C)|FD-Score:-4.57|P-value:2.44E-6|Clearance:0||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:DED81(YHR019C)|FD-Score:-4.45|P-value:4.32E-6|Clearance:0||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:MCM7(YBR202W)|FD-Score:4.65|P-value:1.67E-6|Clearance:0.94||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:PFY1(YOR122C)|FD-Score:-3.65|P-value:1.31E-4|Clearance:0||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:SED5(YLR026C)|FD-Score:-3.25|P-value:5.72E-4|Clearance:0||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins Gene:YGR265W(YGR265W_d)|FD-Score:3.71|P-value:1.05E-4|Clearance:0.81||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YJR023C(YJR023C_d)|FD-Score:-4.35|P-value:6.70E-6|Clearance:0||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing Gene:YOR218C(YOR218C_d)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W Gene:ALG7(YBR243C)|FD-Score:-4.57|P-value:2.42E-6|Clearance:0||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:CAB2(YIL083C)|FD-Score:-4.57|P-value:2.44E-6|Clearance:0||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:DED81(YHR019C)|FD-Score:-4.45|P-value:4.32E-6|Clearance:0||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:MCM7(YBR202W)|FD-Score:4.65|P-value:1.67E-6|Clearance:0.94||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:PFY1(YOR122C)|FD-Score:-3.65|P-value:1.31E-4|Clearance:0||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:SED5(YLR026C)|FD-Score:-3.25|P-value:5.72E-4|Clearance:0||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins Gene:YGR265W(YGR265W_d)|FD-Score:3.71|P-value:1.05E-4|Clearance:0.81||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YJR023C(YJR023C_d)|FD-Score:-4.35|P-value:6.70E-6|Clearance:0||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing Gene:YOR218C(YOR218C_d)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 688802
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIM41(YOR215C)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ASK10(YGR097W)|FD-Score:-3.78|P-value:7.98E-5||SGD DESC:Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication Gene:AUA1(YFL010W-A)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:COQ10(YOL008W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:DOG1(YHR044C)|FD-Score:4.66|P-value:1.60E-6||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified Gene:EAR1(YMR171C)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:ECM33(YBR078W)|FD-Score:5.28|P-value:6.51E-8||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:FMP48(YGR052W_p)|FD-Score:4.36|P-value:6.46E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FRD1(YEL047C)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:FUS1(YCL027W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate Gene:GAL83(YER027C)|FD-Score:5.83|P-value:2.84E-9||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GSY2(YLR258W)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:HHF1(YBR009C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HUA1(YGR268C)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IAH1(YOR126C)|FD-Score:-3.72|P-value:9.98E-5||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:IRC21(YMR073C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:KSS1(YGR040W)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:LPD1(YFL018C)|FD-Score:5.4|P-value:3.41E-8||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:MDH2(YOL126C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MRN1(YPL184C)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MTC2(YKL098W)|FD-Score:5.24|P-value:8.12E-8||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:PAN2(YGL094C)|FD-Score:-3.89|P-value:5.07E-5||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PAN5(YHR063C)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE Gene:PMS1(YNL082W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PPH3(YDR075W)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:PTC7(YHR076W)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:RAD14(YMR201C)|FD-Score:-3.57|P-value:1.75E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAS1(YOR101W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RNH201(YNL072W)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RNH70(YGR276C)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RPS22A(YJL190C)|FD-Score:5.36|P-value:4.28E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:SPI1(YER150W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:TEL1(YBL088C)|FD-Score:-4.06|P-value:2.49E-5||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:TPK1(YJL164C)|FD-Score:-3.6|P-value:1.56E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TRS33(YOR115C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UBC8(YEL012W)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:YDL026W(YDL026W_d)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER039C-A(YER039C-A_p)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YHC3(YJL059W)|FD-Score:-3.1|P-value:9.54E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YIL055C(YIL055C_p)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Putative protein of unknown function Gene:YLR030W(YLR030W_p)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Putative protein of unknown function Gene:YLR419W(YLR419W_p)|FD-Score:3.09|P-value:0.00100||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YLR428C(YLR428C_d)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YNL226W(YNL226W_d)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOL150C(YOL150C_d)|FD-Score:-3.13|P-value:8.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR342C(YOR342C_p)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YPL108W(YPL108W_p)|FD-Score:4.49|P-value:3.50E-6||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPL229W(YPL229W_p)|FD-Score:5.74|P-value:4.73E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPL247C(YPL247C_p)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:ADO1(YJR105W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIM41(YOR215C)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ASK10(YGR097W)|FD-Score:-3.78|P-value:7.98E-5||SGD DESC:Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication Gene:AUA1(YFL010W-A)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:COQ10(YOL008W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:DOG1(YHR044C)|FD-Score:4.66|P-value:1.60E-6||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified Gene:EAR1(YMR171C)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:ECM33(YBR078W)|FD-Score:5.28|P-value:6.51E-8||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:FMP48(YGR052W_p)|FD-Score:4.36|P-value:6.46E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FRD1(YEL047C)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:FUS1(YCL027W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate Gene:GAL83(YER027C)|FD-Score:5.83|P-value:2.84E-9||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GSY2(YLR258W)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:HHF1(YBR009C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HUA1(YGR268C)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IAH1(YOR126C)|FD-Score:-3.72|P-value:9.98E-5||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:IRC21(YMR073C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:KSS1(YGR040W)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:LPD1(YFL018C)|FD-Score:5.4|P-value:3.41E-8||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:MDH2(YOL126C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MRN1(YPL184C)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MTC2(YKL098W)|FD-Score:5.24|P-value:8.12E-8||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:PAN2(YGL094C)|FD-Score:-3.89|P-value:5.07E-5||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PAN5(YHR063C)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE Gene:PMS1(YNL082W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PPH3(YDR075W)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:PTC7(YHR076W)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:RAD14(YMR201C)|FD-Score:-3.57|P-value:1.75E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAS1(YOR101W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RNH201(YNL072W)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RNH70(YGR276C)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RPS22A(YJL190C)|FD-Score:5.36|P-value:4.28E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:SPI1(YER150W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:TEL1(YBL088C)|FD-Score:-4.06|P-value:2.49E-5||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:TPK1(YJL164C)|FD-Score:-3.6|P-value:1.56E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TRS33(YOR115C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UBC8(YEL012W)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:YDL026W(YDL026W_d)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER039C-A(YER039C-A_p)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YHC3(YJL059W)|FD-Score:-3.1|P-value:9.54E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YIL055C(YIL055C_p)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Putative protein of unknown function Gene:YLR030W(YLR030W_p)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Putative protein of unknown function Gene:YLR419W(YLR419W_p)|FD-Score:3.09|P-value:0.00100||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YLR428C(YLR428C_d)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YNL226W(YNL226W_d)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOL150C(YOL150C_d)|FD-Score:-3.13|P-value:8.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR342C(YOR342C_p)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YPL108W(YPL108W_p)|FD-Score:4.49|P-value:3.50E-6||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPL229W(YPL229W_p)|FD-Score:5.74|P-value:4.73E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPL247C(YPL247C_p)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR202W4.651.67E-60.94MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YGR265W_d3.711.05E-40.81YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase
YNL110C2.900.001880.30NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YDL003W2.600.004690.03MCD1Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase
YNR043W2.560.005190.06MVD1Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
YPR137W2.500.006160.01RRP9Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
YLR166C2.500.006260.07SEC10Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion
YDR041W2.430.007510.11RSM10Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
YMR093W2.320.010100.01UTP15Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YDL221W_d2.320.010300.00YDL221W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13
YHR005C-A2.310.010300.02TIM10Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
YHR196W2.300.010800.06UTP9Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YLR075W2.240.012500.02RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YBR198C2.220.013200.05TAF5Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YMR298W2.170.014900.07LIP1Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER027C5.832.84E-9GAL83One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain
YPL229W_p5.744.73E-9YPL229W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication
YFL018C5.403.41E-8LPD1Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes
YJL190C5.364.28E-8RPS22AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication
YBR078W5.286.51E-8ECM33GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p
YKL098W5.248.12E-8MTC2Protein of unknown function; mtc2 is synthetically sick with cdc13-1
YHR044C4.661.60E-6DOG12-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified
YPL247C_p4.532.93E-6YPL247C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest
YPL108W_p4.493.50E-6YPL108W_pCytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
YGR052W_p4.366.46E-6FMP48_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation
YDR075W4.092.11E-5PPH3Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes
YPL184C4.022.96E-5MRN1RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation
YEL047C3.875.47E-5FRD1Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication
YNL082W3.591.67E-4PMS1ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL
YOR342C_p3.462.72E-4YOR342C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1866
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0855.16E-11SGTC_1862st057513 3.8 μMTimTec (Natural product derivative library)53931630.169492
0.0699.78E-8SGTC_29117942208 15.6 μMChembridge (Drug-like library)12433840.177419mitochondrial response to ROS
0.0663.19E-7SGTC_1123mangostin 811.3 nMTimTec (Natural product library)52816500.181818mitochondrial response to ROS
0.0622.00E-6SGTC_1094duloxetine 25.4 μMNIH Clinical Collection608340.0869565RPP1 & pyrimidine depletion
0.0603.38E-6SGTC_1270180-0333 59.1 μMChemDiv (Drug-like library)7716010.125mitochondrial response to ROS
0.0595.66E-6SGTC_18693',7'-dimethoxyflavone 40.3 μMTimTec (Natural product derivative library)6886640.365385
0.0588.31E-6SGTC_1863st057641 20.1 μMTimTec (Natural product derivative library)39216350.183333
0.0588.42E-6SGTC_31159123671 49.5 μMChembridge (Drug-like library)32869880.0952381
0.0571.02E-5SGTC_29659082772 38.6 μMChembridge (Drug-like library)164596340.142857mitochondrial response to ROS
0.0552.50E-5SGTC_687k029-0044 34.9 μMChemDiv (Drug-like library)58293380.0526316PDR1
0.0543.27E-5SGTC_1806st050005 60.3 μMTimTec (Natural product derivative library)51947650.104478
0.0534.37E-5SGTC_30909117696 49.5 μMChembridge (Drug-like library)79238210.0923077
0.0525.59E-5SGTC_28037707263 71.4 μMChembridge (Drug-like library)21992290.16129
0.0518.53E-5SGTC_12400356-0016 42.1 μMChemDiv (Drug-like library)43056130.206349mitochondrial response to ROS
0.0501.17E-4SGTC_14143917-0015 5.4 μMChemDiv (Drug-like library)66187020.0714286

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1867st05737264 μM0.815789688837TimTec (Natural product derivative library)312.316643.08705
SGTC_1833st05598471 μM0.8676288TimTec (Natural product derivative library)282.290663.10304
SGTC_15412',3',6-trimethoxyflavone64 μM0.704545688801TimTec (Pure natural product library)312.316643.08705
SGTC_1111tnp00062510.7 nM0.636364676306TimTec (Natural product library)268.264082.87814
SGTC_1848st05600316.4 μM0.571429146492TimTec (Natural product derivative library)252.264683.1203
SGTC_1727st0343099.9 μM0.568182870305TimTec (Natural product derivative library)293.530483.13403
SGTC_1860st05622874.6 μM0.565217688679TimTec (Natural product derivative library)268.264082.87814
SGTC_1887st05764870.8 μM0.543478688672TimTec (Natural product derivative library)282.290663.10304
SGTC_14204'-methoxyflavone42 μM0.51111177793ChemDiv (Drug-like library)252.264683.1203
SGTC_26344'-methoxyflavone100 μM0.51111177793Microsource (Natural product library)252.264683.1203
SGTC_1857st05600779.3 μM0.510638619834TimTec (Natural product derivative library)252.264683.1203