1488-2104

2-(3-benzyl-4-methyl-1,3-thiazol-3-ium-5-yl)ethyl 2-(3-methyl-1-adamantyl)acetate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_187
Screen concentration 32.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 2852859
SMILES CC1=C(SC=[N+]1CC2=CC=CC=C2)CCOC(=O)CC34CC5CC(C3)CC(C5)(C4)C
Standardized SMILES Cc1c(CCOC(=O)CC23CC4CC(CC(C)(C4)C2)C3)sc[n+]1Cc5ccccc5
Molecular weight 424.6187
ALogP 6.25
H-bond donor count 0
H-bond acceptor count 3
Response signature calcium & mitochondrial duress

Pool Growth Kinetics
% growth inhibition (Het. pool) 23.98
% growth inhibition (Hom. pool) 17.28


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2852859
Download HIP data (tab-delimited text)  (excel)
Gene:CDC20(YGL116W)|FD-Score:3.3|P-value:4.92E-4|Clearance:0.15||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CMD1(YBR109C)|FD-Score:3.38|P-value:3.61E-4|Clearance:0.02||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:DBP2(YNL112W)|FD-Score:-4.06|P-value:2.48E-5|Clearance:0||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:GLC7(YER133W)|FD-Score:5.62|P-value:9.82E-9|Clearance:0.96||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI11(YDR302W)|FD-Score:3.4|P-value:3.41E-4|Clearance:0.02||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:PKC1(YBL105C)|FD-Score:-3.41|P-value:3.27E-4|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRE1(YER012W)|FD-Score:3.45|P-value:2.80E-4|Clearance:0.05||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRE10(YOR362C)|FD-Score:4.43|P-value:4.75E-6|Clearance:0.25||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE2(YPR103W)|FD-Score:4.01|P-value:3.01E-5|Clearance:0.44||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRE5(YMR314W)|FD-Score:8.03|P-value:4.74E-16|Clearance:1.51||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE8(YML092C)|FD-Score:8.46|P-value:1.29E-17|Clearance:1.51||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PUP2(YGR253C)|FD-Score:4.18|P-value:1.48E-5|Clearance:0.16||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:PUP3(YER094C)|FD-Score:6.52|P-value:3.44E-11|Clearance:0.91||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RRP45(YDR280W)|FD-Score:3.36|P-value:3.85E-4|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SCL1(YGL011C)|FD-Score:4.66|P-value:1.60E-6|Clearance:0.23||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC27(YGL137W)|FD-Score:3.15|P-value:8.30E-4|Clearance:0.26||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SEC5(YDR166C)|FD-Score:-3.25|P-value:5.76E-4|Clearance:0||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SPT15(YER148W)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.08||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:TOA2(YKL058W)|FD-Score:3.57|P-value:1.77E-4|Clearance:0.04||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:YNL114C(YNL114C_d)|FD-Score:-3.09|P-value:9.98E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:CDC20(YGL116W)|FD-Score:3.3|P-value:4.92E-4|Clearance:0.15||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CMD1(YBR109C)|FD-Score:3.38|P-value:3.61E-4|Clearance:0.02||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:DBP2(YNL112W)|FD-Score:-4.06|P-value:2.48E-5|Clearance:0||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:GLC7(YER133W)|FD-Score:5.62|P-value:9.82E-9|Clearance:0.96||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI11(YDR302W)|FD-Score:3.4|P-value:3.41E-4|Clearance:0.02||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:PKC1(YBL105C)|FD-Score:-3.41|P-value:3.27E-4|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRE1(YER012W)|FD-Score:3.45|P-value:2.80E-4|Clearance:0.05||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRE10(YOR362C)|FD-Score:4.43|P-value:4.75E-6|Clearance:0.25||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE2(YPR103W)|FD-Score:4.01|P-value:3.01E-5|Clearance:0.44||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRE5(YMR314W)|FD-Score:8.03|P-value:4.74E-16|Clearance:1.51||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE8(YML092C)|FD-Score:8.46|P-value:1.29E-17|Clearance:1.51||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PUP2(YGR253C)|FD-Score:4.18|P-value:1.48E-5|Clearance:0.16||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:PUP3(YER094C)|FD-Score:6.52|P-value:3.44E-11|Clearance:0.91||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RRP45(YDR280W)|FD-Score:3.36|P-value:3.85E-4|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SCL1(YGL011C)|FD-Score:4.66|P-value:1.60E-6|Clearance:0.23||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC27(YGL137W)|FD-Score:3.15|P-value:8.30E-4|Clearance:0.26||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SEC5(YDR166C)|FD-Score:-3.25|P-value:5.76E-4|Clearance:0||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SPT15(YER148W)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.08||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:TOA2(YKL058W)|FD-Score:3.57|P-value:1.77E-4|Clearance:0.04||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:YNL114C(YNL114C_d)|FD-Score:-3.09|P-value:9.98E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2852859
Download HOP data (tab-delimited text)  (excel)
Gene:AFG3(YER017C)|FD-Score:4.95|P-value:3.71E-7||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AIM26(YKL037W)|FD-Score:5.6|P-value:1.07E-8||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:ALG6(YOR002W)|FD-Score:4.5|P-value:3.37E-6||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ALG8(YOR067C)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:BCK1(YJL095W)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BGL2(YGR282C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:CAF40(YNL288W)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CHS6(YJL099W)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CKA1(YIL035C)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CTK3(YML112W)|FD-Score:5.57|P-value:1.29E-8||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CYB5(YNL111C)|FD-Score:8.18|P-value:1.38E-16||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DBP7(YKR024C)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DHH1(YDL160C)|FD-Score:6.16|P-value:3.67E-10||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:EMI2(YDR516C)|FD-Score:-3.25|P-value:5.69E-4||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:ERG2(YMR202W)|FD-Score:6.31|P-value:1.40E-10||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:4.52|P-value:3.12E-6||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERG5(YMR015C)|FD-Score:8.47|P-value:1.25E-17||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FPR1(YNL135C)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:FUR4(YBR021W)|FD-Score:4.81|P-value:7.61E-7||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:HAL5(YJL165C)|FD-Score:23.6|P-value:3.61E-123||SGD DESC:Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication Gene:HMO1(YDR174W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:IMP2'(YIL154C)|FD-Score:15|P-value:3.81E-51||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC15(YPL017C)|FD-Score:3.86|P-value:5.67E-5||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:JIP4(YDR475C)|FD-Score:5.98|P-value:1.14E-9||SGD DESC:Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence Gene:KES1(YPL145C)|FD-Score:7.72|P-value:5.85E-15||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LCB5(YLR260W)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LDB7(YBL006C)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LEM3(YNL323W)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LSM7(YNL147W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:LST4(YKL176C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LST7(YGR057C)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MRPL13(YKR006C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL16(YBL038W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:3.87|P-value:5.39E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:NOT3(YIL038C)|FD-Score:-3.94|P-value:4.00E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NST1(YNL091W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:PET54(YGR222W)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PEX18(YHR160C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PIM1(YBL022C)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PKH2(YOL100W)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PRE9(YGR135W)|FD-Score:5.1|P-value:1.71E-7||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:PSY4(YBL046W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 Gene:RIM1(YCR028C-A)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPA12(YJR063W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL20B(YOR312C)|FD-Score:4.36|P-value:6.64E-6||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPS27B(YHR021C)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:6.12|P-value:4.74E-10||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:-4.15|P-value:1.63E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAT4(YCR008W)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SCH9(YHR205W)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan Gene:SCS2(YER120W)|FD-Score:4|P-value:3.21E-5||SGD DESC:Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication Gene:SLM1(YIL105C)|FD-Score:5.83|P-value:2.72E-9||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication Gene:SLS1(YLR139C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SNF8(YPL002C)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SSD1(YDR293C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSN3(YPL042C)|FD-Score:5.84|P-value:2.66E-9||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SSN8(YNL025C)|FD-Score:8.62|P-value:3.42E-18||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:SSZ1(YHR064C)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:SYT1(YPR095C)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TED1(YIL039W)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TOS1(YBR162C)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TRK1(YJL129C)|FD-Score:11.6|P-value:2.60E-31||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:UBP13(YBL067C)|FD-Score:4.35|P-value:6.90E-6||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:VAM3(YOR106W)|FD-Score:7.25|P-value:2.05E-13||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VOA1(YGR106C)|FD-Score:5.53|P-value:1.62E-8||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:YBP2(YGL060W)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR238C(YBR238C)|FD-Score:-3.49|P-value:2.38E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YDR029W(YDR029W_d)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR442W(YDR442W_d)|FD-Score:4.61|P-value:2.03E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER119C-A(YER119C-A_d)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGL041C(YGL041C_d)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL188C-A(YGL188C-A_p)|FD-Score:6.79|P-value:5.50E-12||SGD DESC:Putative protein of unknown function Gene:YHR080C(YHR080C)|FD-Score:3.86|P-value:5.67E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YLR374C(YLR374C_d)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YML007C-A(YML007C-A_p)|FD-Score:15.3|P-value:3.07E-53||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR031W-A(YMR031W-A_d)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR099C(YMR099C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YMR102C(YMR102C_p)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YNR061C(YNR061C_p)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YPL261C(YPL261C_d)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Gene:ZUO1(YGR285C)|FD-Score:8.96|P-value:1.69E-19||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p Gene:AFG3(YER017C)|FD-Score:4.95|P-value:3.71E-7||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AIM26(YKL037W)|FD-Score:5.6|P-value:1.07E-8||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:ALG6(YOR002W)|FD-Score:4.5|P-value:3.37E-6||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ALG8(YOR067C)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:BCK1(YJL095W)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BGL2(YGR282C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:CAF40(YNL288W)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CHS6(YJL099W)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CKA1(YIL035C)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CTK3(YML112W)|FD-Score:5.57|P-value:1.29E-8||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CYB5(YNL111C)|FD-Score:8.18|P-value:1.38E-16||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DBP7(YKR024C)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DHH1(YDL160C)|FD-Score:6.16|P-value:3.67E-10||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:EMI2(YDR516C)|FD-Score:-3.25|P-value:5.69E-4||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:ERG2(YMR202W)|FD-Score:6.31|P-value:1.40E-10||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:4.52|P-value:3.12E-6||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERG5(YMR015C)|FD-Score:8.47|P-value:1.25E-17||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FPR1(YNL135C)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:FUR4(YBR021W)|FD-Score:4.81|P-value:7.61E-7||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:HAL5(YJL165C)|FD-Score:23.6|P-value:3.61E-123||SGD DESC:Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication Gene:HMO1(YDR174W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:IMP2'(YIL154C)|FD-Score:15|P-value:3.81E-51||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC15(YPL017C)|FD-Score:3.86|P-value:5.67E-5||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:JIP4(YDR475C)|FD-Score:5.98|P-value:1.14E-9||SGD DESC:Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence Gene:KES1(YPL145C)|FD-Score:7.72|P-value:5.85E-15||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LCB5(YLR260W)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LDB7(YBL006C)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LEM3(YNL323W)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LSM7(YNL147W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:LST4(YKL176C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LST7(YGR057C)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MRPL13(YKR006C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL16(YBL038W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:3.87|P-value:5.39E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:NOT3(YIL038C)|FD-Score:-3.94|P-value:4.00E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NST1(YNL091W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:PET54(YGR222W)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PEX18(YHR160C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PIM1(YBL022C)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PKH2(YOL100W)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PRE9(YGR135W)|FD-Score:5.1|P-value:1.71E-7||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:PSY4(YBL046W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 Gene:RIM1(YCR028C-A)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPA12(YJR063W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL20B(YOR312C)|FD-Score:4.36|P-value:6.64E-6||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPS27B(YHR021C)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:6.12|P-value:4.74E-10||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:-4.15|P-value:1.63E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAT4(YCR008W)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SCH9(YHR205W)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan Gene:SCS2(YER120W)|FD-Score:4|P-value:3.21E-5||SGD DESC:Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication Gene:SLM1(YIL105C)|FD-Score:5.83|P-value:2.72E-9||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication Gene:SLS1(YLR139C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SNF8(YPL002C)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SSD1(YDR293C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSN3(YPL042C)|FD-Score:5.84|P-value:2.66E-9||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SSN8(YNL025C)|FD-Score:8.62|P-value:3.42E-18||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:SSZ1(YHR064C)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:SYT1(YPR095C)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TED1(YIL039W)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TOS1(YBR162C)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TRK1(YJL129C)|FD-Score:11.6|P-value:2.60E-31||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:UBP13(YBL067C)|FD-Score:4.35|P-value:6.90E-6||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:VAM3(YOR106W)|FD-Score:7.25|P-value:2.05E-13||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VOA1(YGR106C)|FD-Score:5.53|P-value:1.62E-8||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:YBP2(YGL060W)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR238C(YBR238C)|FD-Score:-3.49|P-value:2.38E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YDR029W(YDR029W_d)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR442W(YDR442W_d)|FD-Score:4.61|P-value:2.03E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER119C-A(YER119C-A_d)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGL041C(YGL041C_d)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL188C-A(YGL188C-A_p)|FD-Score:6.79|P-value:5.50E-12||SGD DESC:Putative protein of unknown function Gene:YHR080C(YHR080C)|FD-Score:3.86|P-value:5.67E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YLR374C(YLR374C_d)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YML007C-A(YML007C-A_p)|FD-Score:15.3|P-value:3.07E-53||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR031W-A(YMR031W-A_d)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR099C(YMR099C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YMR102C(YMR102C_p)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YNR061C(YNR061C_p)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YPL261C(YPL261C_d)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Gene:ZUO1(YGR285C)|FD-Score:8.96|P-value:1.69E-19||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YML092C8.461.29E-171.51PRE8Alpha 2 subunit of the 20S proteasome
YMR314W8.034.74E-161.51PRE5Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YER094C6.523.44E-110.91PUP3Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
YER133W5.629.82E-90.96GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YGL011C4.661.60E-60.23SCL1Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
YOR362C4.434.75E-60.25PRE10Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YGR253C4.181.48E-50.17PUP2Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
YPR103W4.013.01E-50.44PRE2Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
YKL058W3.571.77E-40.04TOA2TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
YER148W3.532.05E-40.08SPT15TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
YER012W3.452.80E-40.05PRE1Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
YDR302W3.403.41E-40.02GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
YBR109C3.383.61E-40.02CMD1Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
YDR280W3.363.85E-40.07RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YGL116W3.304.92E-40.15CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL165C23.603.61E-123HAL5Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
YML007C-A_p15.303.07E-53YML007C-A_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria
YIL154C15.003.81E-51IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YJL129C11.602.60E-31TRK1Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
YGR285C8.961.69E-19ZUO1Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
YNL025C8.623.42E-18SSN8Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance
YMR015C8.471.25E-17ERG5C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
YNL111C8.181.38E-16CYB5Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
YPL145C7.725.85E-15KES1One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
YOR106W7.252.05E-13VAM3Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region
YGL188C-A_p6.795.50E-12YGL188C-A_pPutative protein of unknown function
YMR202W6.311.40E-10ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YDL160C6.163.67E-10DHH1Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress
YKL212W6.124.74E-10SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YDR475C5.981.14E-9JIP4Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence

GO enrichment analysis for SGTC_187
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4515.78E-294SGTC_5820987-0078 156.0 μMChemDiv (Drug-like library)16257650.123596calcium & mitochondrial duress
0.4422.04E-281SGTC_15977-benzyloxygramine 47.5 μMTimTec (Pure natural product library)2608070.130952calcium & mitochondrial duress
0.4414.56E-279SGTC_467ro 31-8220 72.8 μMICCB bioactive library50830.0918367calcium & mitochondrial duress
0.3554.44E-175SGTC_330986-0033 35.8 μMChemDiv (Drug-like library)28480820.126437calcium & mitochondrial duress
0.3515.30E-171SGTC_1712st032288 53.4 μMTimTec (Natural product derivative library)28830110.0705882calcium & mitochondrial duress
0.2964.11E-120SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.101124ERG2
0.2931.26E-117SGTC_502l-744,832 89.3 μMICCB bioactive library66103540.12844calcium & mitochondrial duress
0.2842.28E-110SGTC_5283492-0019 172.0 μMChemDiv (Drug-like library)28692620.126437calcium & mitochondrial duress
0.2811.89E-107SGTC_400oxethazaine 37.5 μMMiscellaneous46210.1625calcium & mitochondrial duress
0.2652.04E-95SGTC_6090986-0026 86.3 μMChemDiv (Drug-like library)28486100.15calcium & mitochondrial duress
0.2571.10E-89SGTC_6583253-0951 102.0 μMChemDiv (Drug-like library)11848780.117647calcium & mitochondrial duress
0.2543.48E-87SGTC_1622st003707 19.4 μMTimTec (Natural product derivative library)101408990.168317calcium & mitochondrial duress
0.2531.27E-86SGTC_1627st003713 25.6 μMTimTec (Natural product derivative library)161950700.156863calcium & mitochondrial duress
0.2493.04E-84SGTC_1812st052057 4.3 μMTimTec (Natural product derivative library)36454180.132653calcium & mitochondrial duress
0.2447.34E-81SGTC_1666st014829 29.0 μMTimTec (Natural product derivative library)38582300.0582524calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_7420kpi-0133140 μM0.2739732728852ChemDiv (Drug-like library)297.434483.98912ERG2
SGTC_12920kpi-0043232 μM0.256413333905ChemDiv (Drug-like library)315.363643.51814
SGTC_9541189-08532.85 μM0.2361112828194ChemDiv (Drug-like library)255.39783.55111endomembrane recycling
SGTC_1260417-1624672.48 μM0.2297341912ChemDiv (Drug-like library)270.366084.07512DNA intercalators
SGTC_360335-088114.2 μM0.22666723828853ChemDiv (Drug-like library)329.477886.29201ubiquinone biosynthesis & proteasome
SGTC_3670335-08817.1 μM0.22666723828853ChemDiv (Drug-like library)329.477886.29201ubiquinone biosynthesis & proteasome
SGTC_3138909721649.47 μM0.22525237486Chembridge (Drug-like library)328.471663.39903
SGTC_442cape100 μM0.2179495281787ICCB bioactive library284.306543.57324
SGTC_13481493-037393.8 μM0.2173913010129ChemDiv (Drug-like library)227.344642.73311
SGTC_6800417-1641114 μM0.2073172835990ChemDiv (Drug-like library)339.471163.54702RPP1 & pyrimidine depletion
SGTC_2649anisomycin9.56 μM0.2051286610309ICCB bioactive library235.278980.89324