amphotericin b FDA approved compound

(3R,4E,6E,8E,10E,12E,14E,16E,18S,19R,20R,21S,37S)-3-[(4R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl]oxy-19,25,27,30,31,33,35,37-octahydroxy-18,20,21-trimethyl-23-oxo-22,39-dioxabicyclo[33.3.1]nonatriaconta-4,6,8,10,12,14,16-heptaene-38-carboxylic acid

Amphotericin b is a polyene antifungal that binds irreversibly to ergosterol, resulting in disruption of membrane integrity and ultimately cell death.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1872
Screen concentration 148.0 nM
Source Microsource (Natural product library)
PubChem CID 16667679
SMILES CC1C=CC=CC=CC=CC=CC=CC=CC(CC2C(C(CC(O2)(CC(CC(C(CCC(CC(CC(=O)OC(C(C1O)C)C)O)O)O)O)O)O)O)C(=O)O)OC3C(C(C(C(O3)C)O)N)O
Standardized SMILES CC1OC(=O)CC(O)CC(O)CCC(O)C(O)CC(O)CC2(O)CC(O)C(C(CC(OC3OC(C)C(O)C(N)C3O)C=CC=CC=CC=CC=CC=CC=CC(C)C(O)C1C)O2)C(=O)O
Molecular weight 924.079
ALogP -2.62
H-bond donor count 12
H-bond acceptor count 18
Response signature RNA pol III & RNase P/MRP

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.49
% growth inhibition (Hom. pool) 5.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 16667679
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:3.8|P-value:7.25E-5|Clearance:0||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARC19(YKL013C)|FD-Score:8.7|P-value:1.65E-18|Clearance:0.43||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARC35(YNR035C)|FD-Score:5.2|P-value:1.00E-7|Clearance:0.22||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:ARP4(YJL081C)|FD-Score:3.41|P-value:3.25E-4|Clearance:0.08||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BET4(YJL031C)|FD-Score:4.12|P-value:1.86E-5|Clearance:0.15||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CAK1(YFL029C)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.06||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC31(YOR257W)|FD-Score:4.83|P-value:6.91E-7|Clearance:0.14||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CDC48(YDL126C)|FD-Score:3.26|P-value:5.53E-4|Clearance:0.12||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:DML1(YMR211W)|FD-Score:11.1|P-value:3.86E-29|Clearance:2.44||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:GCD1(YOR260W)|FD-Score:7.39|P-value:7.38E-14|Clearance:0.41||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD2(YGR083C)|FD-Score:14.1|P-value:2.75E-45|Clearance:2.19||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD6(YDR211W)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.03||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:GCD7(YLR291C)|FD-Score:8.27|P-value:6.60E-17|Clearance:0.76||SGD DESC:Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI14(YJR013W)|FD-Score:5.2|P-value:1.00E-7|Clearance:0||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:INO80(YGL150C)|FD-Score:-3.26|P-value:5.54E-4|Clearance:0||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:POP1(YNL221C)|FD-Score:31.7|P-value:7.27E-221|Clearance:14.11||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:POP3(YNL282W)|FD-Score:7.51|P-value:2.88E-14|Clearance:0.12||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:POP4(YBR257W)|FD-Score:17.6|P-value:1.41E-69|Clearance:0.33||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:POP7(YBR167C)|FD-Score:17.3|P-value:4.17E-67|Clearance:3.19||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RET3(YPL010W)|FD-Score:4.26|P-value:1.00E-5|Clearance:0.14||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPB7(YDR404C)|FD-Score:4.69|P-value:1.35E-6|Clearance:0.3||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC53(YDL150W)|FD-Score:5.54|P-value:1.55E-8|Clearance:0.34||SGD DESC:RNA polymerase III subunit C53 Gene:RPO31(YOR116C)|FD-Score:6.98|P-value:1.53E-12|Clearance:1.44||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:11.9|P-value:6.95E-33|Clearance:0.74||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPR2(YIR015W)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.19||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:SUI2(YJR007W)|FD-Score:4.98|P-value:3.26E-7|Clearance:0.15||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TFC1(YBR123C)|FD-Score:3.79|P-value:7.39E-5|Clearance:0.26||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TOM40(YMR203W)|FD-Score:4.4|P-value:5.52E-6|Clearance:0.13||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YDL152W(YDL152W_d)|FD-Score:3.98|P-value:3.48E-5|Clearance:0.18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YLR339C(YLR339C_d)|FD-Score:-3.53|P-value:2.08E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Gene:YTH1(YPR107C)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.09||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACC1(YNR016C)|FD-Score:3.8|P-value:7.25E-5|Clearance:0||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARC19(YKL013C)|FD-Score:8.7|P-value:1.65E-18|Clearance:0.43||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARC35(YNR035C)|FD-Score:5.2|P-value:1.00E-7|Clearance:0.22||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:ARP4(YJL081C)|FD-Score:3.41|P-value:3.25E-4|Clearance:0.08||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BET4(YJL031C)|FD-Score:4.12|P-value:1.86E-5|Clearance:0.15||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CAK1(YFL029C)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.06||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC31(YOR257W)|FD-Score:4.83|P-value:6.91E-7|Clearance:0.14||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CDC48(YDL126C)|FD-Score:3.26|P-value:5.53E-4|Clearance:0.12||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:DML1(YMR211W)|FD-Score:11.1|P-value:3.86E-29|Clearance:2.44||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:GCD1(YOR260W)|FD-Score:7.39|P-value:7.38E-14|Clearance:0.41||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD2(YGR083C)|FD-Score:14.1|P-value:2.75E-45|Clearance:2.19||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD6(YDR211W)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.03||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:GCD7(YLR291C)|FD-Score:8.27|P-value:6.60E-17|Clearance:0.76||SGD DESC:Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI14(YJR013W)|FD-Score:5.2|P-value:1.00E-7|Clearance:0||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:INO80(YGL150C)|FD-Score:-3.26|P-value:5.54E-4|Clearance:0||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:POP1(YNL221C)|FD-Score:31.7|P-value:7.27E-221|Clearance:14.11||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:POP3(YNL282W)|FD-Score:7.51|P-value:2.88E-14|Clearance:0.12||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:POP4(YBR257W)|FD-Score:17.6|P-value:1.41E-69|Clearance:0.33||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:POP7(YBR167C)|FD-Score:17.3|P-value:4.17E-67|Clearance:3.19||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RET3(YPL010W)|FD-Score:4.26|P-value:1.00E-5|Clearance:0.14||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPB7(YDR404C)|FD-Score:4.69|P-value:1.35E-6|Clearance:0.3||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC53(YDL150W)|FD-Score:5.54|P-value:1.55E-8|Clearance:0.34||SGD DESC:RNA polymerase III subunit C53 Gene:RPO31(YOR116C)|FD-Score:6.98|P-value:1.53E-12|Clearance:1.44||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:11.9|P-value:6.95E-33|Clearance:0.74||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPR2(YIR015W)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.19||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:SUI2(YJR007W)|FD-Score:4.98|P-value:3.26E-7|Clearance:0.15||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TFC1(YBR123C)|FD-Score:3.79|P-value:7.39E-5|Clearance:0.26||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TOM40(YMR203W)|FD-Score:4.4|P-value:5.52E-6|Clearance:0.13||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YDL152W(YDL152W_d)|FD-Score:3.98|P-value:3.48E-5|Clearance:0.18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YLR339C(YLR339C_d)|FD-Score:-3.53|P-value:2.08E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Gene:YTH1(YPR107C)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.09||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 16667679
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ARC18(YLR370C)|FD-Score:3.9|P-value:4.73E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:BRP1(YGL007W_d)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BSD2(YBR290W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification Gene:CAJ1(YER048C)|FD-Score:13.1|P-value:1.11E-39||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CDC50(YCR094W)|FD-Score:7.82|P-value:2.69E-15||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CIN8(YEL061C)|FD-Score:5.32|P-value:5.24E-8||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:COG7(YGL005C)|FD-Score:4.33|P-value:7.58E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DBP3(YGL078C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DEG1(YFL001W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:EMC5(YIL027C)|FD-Score:4.95|P-value:3.62E-7||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:FET4(YMR319C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GET3(YDL100C)|FD-Score:8.39|P-value:2.46E-17||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GRR1(YJR090C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GRX6(YDL010W)|FD-Score:-6.45|P-value:5.67E-11||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:HOP2(YGL033W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HRQ1(YDR291W_p)|FD-Score:-3.93|P-value:4.23E-5||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:HUL4(YJR036C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:IPT1(YDR072C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:KCH1(YJR054W_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate Gene:LDB16(YCL005W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LEM3(YNL323W)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LNP1(YHR192W_p)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MAP1(YLR244C)|FD-Score:-5.14|P-value:1.36E-7||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MCT1(YOR221C)|FD-Score:3.89|P-value:4.99E-5||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MET32(YDR253C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MRN1(YPL184C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MRP2(YPR166C)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NIT1(YIL164C)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:OSH3(YHR073W)|FD-Score:5.42|P-value:2.94E-8||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAI3(YMR174C)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact Gene:PEX3(YDR329C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PHO5(YBR093C)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 Gene:PHO88(YBR106W)|FD-Score:5.01|P-value:2.79E-7||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PMP3(YDR276C)|FD-Score:11.8|P-value:1.11E-32||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PRM8(YGL053W)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family Gene:PSH1(YOL054W)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p Gene:PTK2(YJR059W)|FD-Score:8.81|P-value:6.09E-19||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RCY1(YJL204C)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:SAC6(YDR129C)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDT1(YGL224C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SHE4(YOR035C)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIF2(YBR103W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SRO77(YBL106C)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SWA2(YDR320C)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TRK1(YJL129C)|FD-Score:-4.31|P-value:8.31E-6||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRM11(YOL124C)|FD-Score:7.69|P-value:7.60E-15||SGD DESC:Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Gene:TSC3(YBR058C-A)|FD-Score:6.05|P-value:7.20E-10||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:ULA1(YPL003W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR016W(YCR016W_p)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YDR491C(YDR491C_d)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL013W-A(YFL013W-A_d)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YHR175W-A(YHR175W-A_p)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJR079W(YJR079W_p)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YML122C(YML122C_d)|FD-Score:7.51|P-value:2.92E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR052C-A(YMR052C-A_d)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR102C(YMR102C_p)|FD-Score:7.22|P-value:2.59E-13||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YNR029C(YNR029C_p)|FD-Score:5.88|P-value:1.99E-9||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOR052C(YOR052C)|FD-Score:5.73|P-value:4.88E-9||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YPK1(YKL126W)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YSP2(YDR326C)|FD-Score:9.4|P-value:2.69E-21||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:ZAP1(YJL056C)|FD-Score:5.56|P-value:1.38E-8||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:AAT2(YLR027C)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ARC18(YLR370C)|FD-Score:3.9|P-value:4.73E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:BRP1(YGL007W_d)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BSD2(YBR290W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification Gene:CAJ1(YER048C)|FD-Score:13.1|P-value:1.11E-39||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CDC50(YCR094W)|FD-Score:7.82|P-value:2.69E-15||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CIN8(YEL061C)|FD-Score:5.32|P-value:5.24E-8||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:COG7(YGL005C)|FD-Score:4.33|P-value:7.58E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DBP3(YGL078C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DEG1(YFL001W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:EMC5(YIL027C)|FD-Score:4.95|P-value:3.62E-7||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:FET4(YMR319C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GET3(YDL100C)|FD-Score:8.39|P-value:2.46E-17||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GRR1(YJR090C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GRX6(YDL010W)|FD-Score:-6.45|P-value:5.67E-11||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:HOP2(YGL033W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HRQ1(YDR291W_p)|FD-Score:-3.93|P-value:4.23E-5||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:HUL4(YJR036C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:IPT1(YDR072C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:KCH1(YJR054W_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate Gene:LDB16(YCL005W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LEM3(YNL323W)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LNP1(YHR192W_p)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MAP1(YLR244C)|FD-Score:-5.14|P-value:1.36E-7||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MCT1(YOR221C)|FD-Score:3.89|P-value:4.99E-5||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MET32(YDR253C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MRN1(YPL184C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MRP2(YPR166C)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NIT1(YIL164C)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:OSH3(YHR073W)|FD-Score:5.42|P-value:2.94E-8||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAI3(YMR174C)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact Gene:PEX3(YDR329C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PHO5(YBR093C)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 Gene:PHO88(YBR106W)|FD-Score:5.01|P-value:2.79E-7||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PMP3(YDR276C)|FD-Score:11.8|P-value:1.11E-32||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PRM8(YGL053W)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family Gene:PSH1(YOL054W)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p Gene:PTK2(YJR059W)|FD-Score:8.81|P-value:6.09E-19||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RCY1(YJL204C)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:SAC6(YDR129C)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDT1(YGL224C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SHE4(YOR035C)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIF2(YBR103W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SRO77(YBL106C)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SWA2(YDR320C)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TRK1(YJL129C)|FD-Score:-4.31|P-value:8.31E-6||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRM11(YOL124C)|FD-Score:7.69|P-value:7.60E-15||SGD DESC:Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Gene:TSC3(YBR058C-A)|FD-Score:6.05|P-value:7.20E-10||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:ULA1(YPL003W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR016W(YCR016W_p)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YDR491C(YDR491C_d)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL013W-A(YFL013W-A_d)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YHR175W-A(YHR175W-A_p)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJR079W(YJR079W_p)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YML122C(YML122C_d)|FD-Score:7.51|P-value:2.92E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR052C-A(YMR052C-A_d)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR102C(YMR102C_p)|FD-Score:7.22|P-value:2.59E-13||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YNR029C(YNR029C_p)|FD-Score:5.88|P-value:1.99E-9||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOR052C(YOR052C)|FD-Score:5.73|P-value:4.88E-9||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YPK1(YKL126W)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YSP2(YDR326C)|FD-Score:9.4|P-value:2.69E-21||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:ZAP1(YJL056C)|FD-Score:5.56|P-value:1.38E-8||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL221C31.707.27E-22114.10POP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YBR257W17.601.41E-690.33POP4Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YBR167C17.304.17E-673.19POP7Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YGR083C14.102.75E-452.19GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YHR062C11.906.95E-330.74RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YMR211W11.103.86E-292.44DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YKL013C8.701.65E-180.43ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YLR291C8.276.60E-170.76GCD7Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YNL282W7.512.88E-140.12POP3Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YOR260W7.397.38E-140.41GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YOR116C6.981.53E-121.44RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YDL150W5.541.55E-80.34RPC53RNA polymerase III subunit C53
YJR013W5.201.00E-72.24E-4GPI14Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
YNR035C5.201.00E-70.22ARC35Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
YJR007W4.983.26E-70.15SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER048C13.101.11E-39CAJ1Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly
YDR276C11.801.11E-32PMP3Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential
YDR326C9.402.69E-21YSP2Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication
YJR059W8.816.09E-19PTK2Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake
YDL100C8.392.46E-17GET3Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
YCR094W7.822.69E-15CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YOL124C7.697.60E-15TRM11Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain
YML122C_d7.512.92E-14YML122C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR102C_p7.222.59E-13YMR102C_pProtein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene
YBR058C-A6.057.20E-10TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YNR029C_p5.881.99E-9YNR029C_pPutative protein of unknown function, deletion confers reduced fitness in saline
YOR052C5.734.88E-9YOR052CNuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress
YJL056C5.561.38E-8ZAP1Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains
YHR073W5.422.94E-8OSH3Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
YEL061C5.325.24E-8CIN8Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation

GO enrichment analysis for SGTC_1872
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4432.75E-282SGTC_2411st077133 39.9 μMTimTec (Natural product derivative library)14031420.0789474RNA pol III & RNase P/MRP
0.3988.93E-224SGTC_5394859-0462 2.5 μMChemDiv (Drug-like library)464994740.0247934RNA pol III & RNase P/MRP
0.3145.35E-135SGTC_2612dictamnine 15.5 μMMicrosource (Natural product library)680850.047619RNA pol III & RNase P/MRP
0.2783.25E-105SGTC_10164226-1401 38.0 μMChemDiv (Drug-like library)29016130.10084RNA pol III & RNase P/MRP
0.2604.32E-92SGTC_2685mycophenolic acid 18.9 μMMiscellaneous4465410.0940171RNA pol III & RNase P/MRP
0.2513.22E-85SGTC_919tnp00227 403.6 nMTimTec (Natural product library)12692460.0762712RNA pol III & RNase P/MRP
0.2181.99E-64SGTC_15346-azauridine 81.6 μMTimTec (Pure natural product library)2335020.0943396RNA pol III & RNase P/MRP
0.2121.04E-60SGTC_21565649887 115.0 μMChembridge (Fragment library)54680440NARPP1 & pyrimidine depletion
0.2094.02E-59SGTC_5481436-0017 110.0 μMChemDiv (Drug-like library)96771370.0666667TSC3-RPN4
0.2042.94E-56SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.0672269
0.1911.05E-49SGTC_20575236571 38.3 μMChembridge (Fragment library)54684039NARPP1 & pyrimidine depletion
0.1825.84E-45SGTC_1568digitonin 820.0 nMTimTec (Pure natural product library)27350100.129252sphingolipid biosynthesis & PDR1
0.1805.49E-44SGTC_2544sphondin 68.5 μMMicrosource (Natural product library)1081040.0648148RNA pol III & RNase P/MRP
0.1751.03E-41SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0571429TSC3-RPN4
0.1693.48E-39SGTC_266formosanin C 406.0 nMMiscellaneous735970.170213sphingolipid biosynthesis & PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2663amphotericin b100 μM116667679Microsource (Natural product library)924.07902-2.6171218
SGTC_2657nystatin74.44 μM0.67021311468707Miscellaneous926.0949-2.6041218
SGTC_277nystatin15.5 μM0.67021311468707Miscellaneous926.0949-2.6041218
SGTC_1574spiramycin23.7 μM0.2285716419898TimTec (Pure natural product library)843.052662.049416
SGTC_1578josamycin24.2 μM0.2176875282165TimTec (Pure natural product library)827.994962.957316
SGTC_1079idarubicin2.36 μM0.215517636362NIH Clinical Collection533.954821.026610DNA damage response
SGTC_2665lovastatin100 μM0.20689753232Miscellaneous404.539644.21815
SGTC_504lovastatin124 μM0.20689753232ICCB bioactive library404.539644.21815
SGTC_1545lovastatin49.4 μM0.20689712923612TimTec (Pure natural product library)404.539644.21815
SGTC_257daunorubicin18.5 μM0.20161330323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_2662daunorubicin22.37 μM0.20161330323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_2746daunorubicin9.88 μM0.20161330323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_272doxorubicin8.13 μM0.1968531703Miscellaneous543.51926-0.044612anthracycline transcription coupled DNA repair
SGTC_2747doxorubicin7.63 μM0.1968531703Miscellaneous543.51926-0.044612anthracycline transcription coupled DNA repair
SGTC_1085doxorubicin7.67 μM0.19685443939NIH Clinical Collection579.98020.338712anthracycline transcription coupled DNA repair
SGTC_1105epirubicin18.1 μM0.1968565348NIH Clinical Collection579.98020.338712anthracycline transcription coupled DNA repair
SGTC_2611pyromycin23.93 μM0.178295196990Microsource (Natural product library)585.5991.992512