myriocin

2-amino-3,4-dihydroxy-2-(hydroxymethyl)-14-oxoicos-6-enoic acid


Established Yeast Drug Target : LCB1 LCB2

Myriocin is a very potent inhibitor of serine palmitoyltransferase, the first step in sphingosine biosynthesis.[2] Due to this property, it is used in biochemical research as a tool for depleting cells of sphingolipids.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1876
Screen concentration 605.0 nM
Source Miscellaneous
PubChem CID 301119
SMILES CCCCCCC(=O)CCCCCCC=CCC(C(C(CO)(C(=O)O)N)O)O
Standardized SMILES CCCCCCC(=O)CCCCCCC=CCC(O)C(O)C(N)(CO)C(=O)O
Molecular weight 401.5375
ALogP 0.28
H-bond donor count 5
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.41
% growth inhibition (Hom. pool) 1.02


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 301119
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:-5.53|P-value:1.63E-8|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:FAS1(YKL182W)|FD-Score:5.78|P-value:3.67E-9|Clearance:0.19||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:HRP1(YOL123W)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.01||SGD DESC:Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences Gene:JAC1(YGL018C)|FD-Score:3.42|P-value:3.16E-4|Clearance:0.01||SGD DESC:Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix Gene:LCB1(YMR296C)|FD-Score:18.6|P-value:1.54E-77|Clearance:10.68||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LCB2(YDR062W)|FD-Score:7.92|P-value:1.16E-15|Clearance:0.72||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:NOP15(YNL110C)|FD-Score:-4.08|P-value:2.23E-5|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NUP145(YGL092W)|FD-Score:5.59|P-value:1.15E-8|Clearance:1.63||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:PKC1(YBL105C)|FD-Score:-3.56|P-value:1.86E-4|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRP3(YDR473C)|FD-Score:-3.49|P-value:2.38E-4|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPB7(YDR404C)|FD-Score:-3.15|P-value:8.18E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPL25(YOL127W)|FD-Score:3.35|P-value:4.06E-4|Clearance:0.05||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPO26(YPR187W)|FD-Score:-4.83|P-value:6.75E-7|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPP1(YHR062C)|FD-Score:-3.18|P-value:7.41E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS13(YDR064W)|FD-Score:3.43|P-value:3.06E-4|Clearance:0.01||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:SEC24(YIL109C)|FD-Score:3.3|P-value:4.87E-4|Clearance:0.21||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SPB4(YFL002C)|FD-Score:-3.29|P-value:5.06E-4|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TCP1(YDR212W)|FD-Score:3.41|P-value:3.25E-4|Clearance:0.02||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TUB1(YML085C)|FD-Score:7.2|P-value:3.00E-13|Clearance:1.42||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YJL032W(YJL032W_d)|FD-Score:-3.62|P-value:1.45E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase Gene:YJL195C(YJL195C_d)|FD-Score:3.96|P-value:3.72E-5|Clearance:0.47||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YJU2(YKL095W)|FD-Score:3.39|P-value:3.52E-4|Clearance:0.04||SGD DESC:Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing Gene:YLR101C(YLR101C_d)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W Gene:ACT1(YFL039C)|FD-Score:-5.53|P-value:1.63E-8|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:FAS1(YKL182W)|FD-Score:5.78|P-value:3.67E-9|Clearance:0.19||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:HRP1(YOL123W)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.01||SGD DESC:Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences Gene:JAC1(YGL018C)|FD-Score:3.42|P-value:3.16E-4|Clearance:0.01||SGD DESC:Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix Gene:LCB1(YMR296C)|FD-Score:18.6|P-value:1.54E-77|Clearance:10.68||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LCB2(YDR062W)|FD-Score:7.92|P-value:1.16E-15|Clearance:0.72||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:NOP15(YNL110C)|FD-Score:-4.08|P-value:2.23E-5|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NUP145(YGL092W)|FD-Score:5.59|P-value:1.15E-8|Clearance:1.63||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:PKC1(YBL105C)|FD-Score:-3.56|P-value:1.86E-4|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRP3(YDR473C)|FD-Score:-3.49|P-value:2.38E-4|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPB7(YDR404C)|FD-Score:-3.15|P-value:8.18E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPL25(YOL127W)|FD-Score:3.35|P-value:4.06E-4|Clearance:0.05||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPO26(YPR187W)|FD-Score:-4.83|P-value:6.75E-7|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPP1(YHR062C)|FD-Score:-3.18|P-value:7.41E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS13(YDR064W)|FD-Score:3.43|P-value:3.06E-4|Clearance:0.01||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:SEC24(YIL109C)|FD-Score:3.3|P-value:4.87E-4|Clearance:0.21||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SPB4(YFL002C)|FD-Score:-3.29|P-value:5.06E-4|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TCP1(YDR212W)|FD-Score:3.41|P-value:3.25E-4|Clearance:0.02||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TUB1(YML085C)|FD-Score:7.2|P-value:3.00E-13|Clearance:1.42||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YJL032W(YJL032W_d)|FD-Score:-3.62|P-value:1.45E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase Gene:YJL195C(YJL195C_d)|FD-Score:3.96|P-value:3.72E-5|Clearance:0.47||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YJU2(YKL095W)|FD-Score:3.39|P-value:3.52E-4|Clearance:0.04||SGD DESC:Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing Gene:YLR101C(YLR101C_d)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 301119
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ANB1(YJR047C)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication Gene:ATP11(YNL315C)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:BLM10(YFL007W)|FD-Score:3.73|P-value:9.55E-5||SGD DESC:Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 Gene:BMH1(YER177W)|FD-Score:6.71|P-value:9.97E-12||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CAP2(YIL034C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CHS3(YBR023C)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:COG5(YNL051W)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-4.9|P-value:4.88E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTR9(YOL145C)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:CUS2(YNL286W)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:DBP3(YGL078C)|FD-Score:-3.84|P-value:6.05E-5||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DEG1(YFL001W)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DGA1(YOR245C)|FD-Score:4.28|P-value:9.34E-6||SGD DESC:Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles Gene:DPH5(YLR172C)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:DSE4(YNR067C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:END3(YNL084C)|FD-Score:4.71|P-value:1.22E-6||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:FET4(YMR319C)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FLR1(YBR008C)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:FYV10(YIL097W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:FYV4(YHR059W)|FD-Score:6.28|P-value:1.74E-10||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAL11(YOL051W)|FD-Score:4.38|P-value:5.98E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GAL83(YER027C)|FD-Score:3.86|P-value:5.63E-5||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GIS2(YNL255C)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:HHF1(YBR009C)|FD-Score:-3.72|P-value:9.89E-5||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HNT3(YOR258W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:INM1(YHR046C)|FD-Score:5.87|P-value:2.18E-9||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:IRC21(YMR073C)|FD-Score:-3.33|P-value:4.31E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LAC1(YKL008C)|FD-Score:4.68|P-value:1.42E-6||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:LCB3(YJL134W)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Long-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication Gene:LCL2(YLR104W_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:LEM3(YNL323W)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MCK1(YNL307C)|FD-Score:5.47|P-value:2.24E-8||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MDM20(YOL076W)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MDM34(YGL219C)|FD-Score:-4.17|P-value:1.54E-5||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRPL27(YBR282W)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MSC2(YDR205W)|FD-Score:3.85|P-value:5.91E-5||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MSN1(YOL116W)|FD-Score:3.88|P-value:5.33E-5||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MTC2(YKL098W)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:PAA1(YDR071C)|FD-Score:20.8|P-value:4.43E-96||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PGD1(YGL025C)|FD-Score:4.63|P-value:1.83E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO80(YOL001W)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PHO84(YML123C)|FD-Score:-3.83|P-value:6.48E-5||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PHO86(YJL117W)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PKH2(YOL100W)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PRM8(YGL053W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family Gene:PRO2(YOR323C)|FD-Score:5.74|P-value:4.79E-9||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTC4(YBR125C)|FD-Score:11.4|P-value:1.38E-30||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:RCK2(YLR248W)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RGP1(YDR137W)|FD-Score:-3.88|P-value:5.32E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:ROG3(YFR022W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication Gene:ROM2(YLR371W)|FD-Score:-4.46|P-value:4.09E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL19A(YBR084C-A)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL31A(YDL075W)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPL34A(YER056C-A)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:-3.28|P-value:5.15E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RTA1(YGR213C)|FD-Score:10.9|P-value:6.88E-28||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:RVS161(YCR009C)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAC1(YKL212W)|FD-Score:-5.71|P-value:5.74E-9||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SEA4(YBL104C)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SKI8(YGL213C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SLI1(YGR212W)|FD-Score:40.4|P-value:0||SGD DESC:N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin Gene:SLM1(YIL105C)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication Gene:SRB2(YHR041C)|FD-Score:12.4|P-value:8.52E-36||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SRB5(YGR104C)|FD-Score:7.21|P-value:2.73E-13||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SSK2(YNR031C)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:SST2(YLR452C)|FD-Score:-3.14|P-value:8.33E-4||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:STL1(YDR536W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock Gene:TAT1(YBR069C)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TIF1(YKR059W)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication Gene:TPM1(YNL079C)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TRE1(YPL176C)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:11.4|P-value:1.19E-30||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP3(YER151C)|FD-Score:6.23|P-value:2.28E-10||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:UPF3(YGR072W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:VPS61(YDR136C_d)|FD-Score:-4.64|P-value:1.71E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS64(YDR200C)|FD-Score:-3.79|P-value:7.55E-5||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:VPS69(YPR087W_d)|FD-Score:4.44|P-value:4.48E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YDL027C(YDL027C_p)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene Gene:YDL118W(YDL118W_p)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR094W(YDR094W_d)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YER130C(YER130C_p)|FD-Score:9.95|P-value:1.26E-23||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YGL007C-A(YGL007C-A_p)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGR139W(YGR139W_d)|FD-Score:3.99|P-value:3.26E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL213W(YJL213W)|FD-Score:6.42|P-value:6.70E-11||SGD DESC:Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p Gene:YLR111W(YLR111W_d)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR279W(YLR279W_d)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR052C-A(YMR052C-A_d)|FD-Score:3.75|P-value:8.74E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR084W(YMR084W_p)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR153C-A(YMR153C-A_d)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YOL050C(YOL050C_d)|FD-Score:7.12|P-value:5.21E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR225W(YOR225W_d)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:10.6|P-value:1.42E-26||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:-4.39|P-value:5.64E-6||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ADO1(YJR105W)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ANB1(YJR047C)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication Gene:ATP11(YNL315C)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:BLM10(YFL007W)|FD-Score:3.73|P-value:9.55E-5||SGD DESC:Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 Gene:BMH1(YER177W)|FD-Score:6.71|P-value:9.97E-12||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CAP2(YIL034C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CHS3(YBR023C)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:COG5(YNL051W)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-4.9|P-value:4.88E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTR9(YOL145C)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:CUS2(YNL286W)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:DBP3(YGL078C)|FD-Score:-3.84|P-value:6.05E-5||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DEG1(YFL001W)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DGA1(YOR245C)|FD-Score:4.28|P-value:9.34E-6||SGD DESC:Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles Gene:DPH5(YLR172C)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:DSE4(YNR067C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:END3(YNL084C)|FD-Score:4.71|P-value:1.22E-6||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:FET4(YMR319C)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FLR1(YBR008C)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:FYV10(YIL097W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:FYV4(YHR059W)|FD-Score:6.28|P-value:1.74E-10||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAL11(YOL051W)|FD-Score:4.38|P-value:5.98E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GAL83(YER027C)|FD-Score:3.86|P-value:5.63E-5||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GIS2(YNL255C)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:HHF1(YBR009C)|FD-Score:-3.72|P-value:9.89E-5||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HNT3(YOR258W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:INM1(YHR046C)|FD-Score:5.87|P-value:2.18E-9||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:IRC21(YMR073C)|FD-Score:-3.33|P-value:4.31E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LAC1(YKL008C)|FD-Score:4.68|P-value:1.42E-6||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:LCB3(YJL134W)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Long-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication Gene:LCL2(YLR104W_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:LEM3(YNL323W)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MCK1(YNL307C)|FD-Score:5.47|P-value:2.24E-8||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MDM20(YOL076W)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MDM34(YGL219C)|FD-Score:-4.17|P-value:1.54E-5||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRPL27(YBR282W)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MSC2(YDR205W)|FD-Score:3.85|P-value:5.91E-5||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MSN1(YOL116W)|FD-Score:3.88|P-value:5.33E-5||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MTC2(YKL098W)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:PAA1(YDR071C)|FD-Score:20.8|P-value:4.43E-96||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PGD1(YGL025C)|FD-Score:4.63|P-value:1.83E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO80(YOL001W)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PHO84(YML123C)|FD-Score:-3.83|P-value:6.48E-5||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PHO86(YJL117W)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PKH2(YOL100W)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PRM8(YGL053W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family Gene:PRO2(YOR323C)|FD-Score:5.74|P-value:4.79E-9||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTC4(YBR125C)|FD-Score:11.4|P-value:1.38E-30||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:RCK2(YLR248W)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RGP1(YDR137W)|FD-Score:-3.88|P-value:5.32E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:ROG3(YFR022W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication Gene:ROM2(YLR371W)|FD-Score:-4.46|P-value:4.09E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL19A(YBR084C-A)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL31A(YDL075W)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPL34A(YER056C-A)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:-3.28|P-value:5.15E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RTA1(YGR213C)|FD-Score:10.9|P-value:6.88E-28||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:RVS161(YCR009C)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAC1(YKL212W)|FD-Score:-5.71|P-value:5.74E-9||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SEA4(YBL104C)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SKI8(YGL213C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SLI1(YGR212W)|FD-Score:40.4|P-value:0||SGD DESC:N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin Gene:SLM1(YIL105C)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication Gene:SRB2(YHR041C)|FD-Score:12.4|P-value:8.52E-36||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SRB5(YGR104C)|FD-Score:7.21|P-value:2.73E-13||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SSK2(YNR031C)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:SST2(YLR452C)|FD-Score:-3.14|P-value:8.33E-4||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:STL1(YDR536W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock Gene:TAT1(YBR069C)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TIF1(YKR059W)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication Gene:TPM1(YNL079C)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TRE1(YPL176C)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:11.4|P-value:1.19E-30||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP3(YER151C)|FD-Score:6.23|P-value:2.28E-10||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:UPF3(YGR072W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:VPS61(YDR136C_d)|FD-Score:-4.64|P-value:1.71E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS64(YDR200C)|FD-Score:-3.79|P-value:7.55E-5||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:VPS69(YPR087W_d)|FD-Score:4.44|P-value:4.48E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YDL027C(YDL027C_p)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene Gene:YDL118W(YDL118W_p)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR094W(YDR094W_d)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YER130C(YER130C_p)|FD-Score:9.95|P-value:1.26E-23||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YGL007C-A(YGL007C-A_p)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGR139W(YGR139W_d)|FD-Score:3.99|P-value:3.26E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL213W(YJL213W)|FD-Score:6.42|P-value:6.70E-11||SGD DESC:Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p Gene:YLR111W(YLR111W_d)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR279W(YLR279W_d)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR052C-A(YMR052C-A_d)|FD-Score:3.75|P-value:8.74E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR084W(YMR084W_p)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR153C-A(YMR153C-A_d)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YOL050C(YOL050C_d)|FD-Score:7.12|P-value:5.21E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR225W(YOR225W_d)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:10.6|P-value:1.42E-26||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:-4.39|P-value:5.64E-6||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR296C18.601.54E-7710.70LCB1Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YDR062W7.921.16E-150.72LCB2Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YML085C7.203.00E-131.42TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YKL182W5.783.67E-90.20FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YGL092W5.591.15E-81.63NUP145Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98
YJL195C_d3.963.72E-50.47YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YOL123W3.492.43E-40.01HRP1Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences
YLR101C_d3.482.48E-40.06YLR101C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W
YDR064W3.433.06E-40.01RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
YGL018C3.423.16E-40.01JAC1Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix
YDR212W3.413.25E-40.02TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YKL095W3.393.52E-40.04YJU2Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing
YOL127W3.354.06E-40.05RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YIL109C3.304.87E-40.21SEC24Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p
YPL251W_d3.090.001010.02YPL251W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR212W40.400SLI1N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin
YDR071C20.804.43E-96PAA1Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication
YHR041C12.408.52E-36SRB2Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance
YBR058C-A11.401.19E-30TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YBR125C11.401.38E-30PTC4Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity
YGR213C10.906.88E-28RTA1Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein
YKL126W10.601.42E-26YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YER130C_p9.951.26E-23YER130C_pProtein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress
YGR104C7.212.73E-13SRB5Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance
YOL050C_d7.125.21E-13YOL050C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
YER177W6.719.97E-12BMH114-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
YJL213W6.426.70E-11YJL213WProtein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p
YHR059W6.281.74E-10FYV4Protein of unknown function, required for survival upon exposure to K1 killer toxin
YER151C6.232.28E-10UBP3Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress
YHR046C5.872.18E-9INM1Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate

GO enrichment analysis for SGTC_1876
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1411.54E-27SGTC_6710560-0003 407.0 μMChemDiv (Drug-like library)32386780.0821918
0.1352.24E-25SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.05TSC3-RPN4
0.1345.38E-25SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0136986TSC3-RPN4
0.1314.62E-24SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.045977TSC3-RPN4
0.1301.35E-23SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0123457TSC3-RPN4
0.1255.70E-22SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.0681818TSC3-RPN4
0.1248.70E-22SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0277778TSC3-RPN4
0.1217.31E-21SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.0714286TSC3-RPN4
0.1171.66E-19SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0972222TSC3-RPN4
0.1172.10E-19SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.0561798TSC3-RPN4
0.1159.21E-19SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.0379747TSC3-RPN4
0.1134.39E-18SGTC_22657929201 200.0 μMChembridge (Fragment library)12651080.0540541
0.1102.06E-17SGTC_22176934799 96.1 μMChembridge (Fragment library)6756060.136364TSC3-RPN4
0.1087.93E-17SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0555556TSC3-RPN4
0.1062.92E-16SGTC_6641222-0054 14.9 μMChemDiv (Drug-like library)68139910.123288sphingolipid biosynthesis & PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_476mead acid10 μM0.45312531ICCB bioactive library306.482766.88312
SGTC_1617st00290678 μM0.3829795150859TimTec (Natural product derivative library)256.381024.51413
SGTC_409arachidonic acid100 μM0.38231Miscellaneous304.466886.43912
SGTC_479n-linoleoylglycine10 μM0.3756433346ICCB bioactive library337.49685.47623
SGTC_4732-arachidonoylglycerol10 μM0.3508775282280ICCB bioactive library378.545425.61424
SGTC_477mead ethanolamide10 μM0.35087716061185ICCB bioactive library349.550565.92222
SGTC_410α-linolenic acid100 μM0.346154860Miscellaneous278.42965.97112excess fatty acid
SGTC_475α-linolenic acid10 μM0.3461545280934ICCB bioactive library278.42965.97112excess fatty acid
SGTC_438sphingosine6.67 μM0.3396235280335ICCB bioactive library299.491884.82433
SGTC_478N-arachidonylglycine10 μM0.3333335283389ICCB bioactive library361.51825.49923
SGTC_1573oleylamine1.06 μM0.3191495356789TimTec (Pure natural product library)267.493086.62311