wortmannin

A covalent inhibitor of PI 3-kinase.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1877
Screen concentration 25.0 μM
Source ICCB bioactive library
PubChem CID 312145
SMILES CC(=O)OC1CC2(C(CCC2=O)C3=C1C4(C(OC(=O)C5=COC(=C54)C3=O)COC)C)C
Standardized SMILES COCC1OC(=O)c2coc3C(=O)C4=C(C(CC5(C)C4CCC5=O)OC(=O)C)C1(C)c23
Molecular weight 428.4319
ALogP 1.71
H-bond donor count 0
H-bond acceptor count 8
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.54
% growth inhibition (Hom. pool) 6.26


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 312145
Download HIP data (tab-delimited text)  (excel)
Gene:ARC19(YKL013C)|FD-Score:4.48|P-value:3.72E-6|Clearance:0.17||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARC35(YNR035C)|FD-Score:3.25|P-value:5.83E-4|Clearance:0.03||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:ARP3(YJR065C)|FD-Score:3.54|P-value:1.99E-4|Clearance:0.11||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CDC20(YGL116W)|FD-Score:7.5|P-value:3.07E-14|Clearance:1.79||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:DRS1(YLL008W)|FD-Score:-4.64|P-value:1.75E-6|Clearance:0||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:ERG11(YHR007C)|FD-Score:4.29|P-value:9.08E-6|Clearance:0.24||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GLC7(YER133W)|FD-Score:-3.33|P-value:4.40E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI14(YJR013W)|FD-Score:4.96|P-value:3.51E-7|Clearance:0.12||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:HRR25(YPL204W)|FD-Score:-3.5|P-value:2.29E-4|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:KOG1(YHR186C)|FD-Score:11.9|P-value:7.61E-33|Clearance:4.37||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:PKC1(YBL105C)|FD-Score:4.31|P-value:8.07E-6|Clearance:0.03||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RPA43(YOR340C)|FD-Score:4.05|P-value:2.56E-5|Clearance:0.16||SGD DESC:RNA polymerase I subunit A43 Gene:RPB8(YOR224C)|FD-Score:3.21|P-value:6.56E-4|Clearance:0.05||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC82(YPR190C)|FD-Score:3.43|P-value:3.05E-4|Clearance:0.16||SGD DESC:RNA polymerase III subunit C82 Gene:RPL25(YOL127W)|FD-Score:4.84|P-value:6.51E-7|Clearance:0.36||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RSM10(YDR041W)|FD-Score:5.72|P-value:5.35E-9|Clearance:0.39||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins Gene:SPC97(YHR172W)|FD-Score:3.26|P-value:5.51E-4|Clearance:0.02||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SUI2(YJR007W)|FD-Score:5.33|P-value:4.90E-8|Clearance:0.29||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TAF5(YBR198C)|FD-Score:3.13|P-value:8.77E-4|Clearance:0.08||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TSC11(YER093C)|FD-Score:3.89|P-value:4.99E-5|Clearance:0.09||SGD DESC:Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain Gene:TUB1(YML085C)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.17||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UGP1(YKL035W)|FD-Score:-4.14|P-value:1.77E-5|Clearance:0||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:YJL086C(YJL086C_d)|FD-Score:5.04|P-value:2.28E-7|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR230W(YLR230W_d)|FD-Score:-4.62|P-value:1.91E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:YOR146W(YOR146W_d)|FD-Score:3.8|P-value:7.16E-5|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPL238C(YPL238C_d)|FD-Score:3.16|P-value:7.90E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:ARC19(YKL013C)|FD-Score:4.48|P-value:3.72E-6|Clearance:0.17||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARC35(YNR035C)|FD-Score:3.25|P-value:5.83E-4|Clearance:0.03||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:ARP3(YJR065C)|FD-Score:3.54|P-value:1.99E-4|Clearance:0.11||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CDC20(YGL116W)|FD-Score:7.5|P-value:3.07E-14|Clearance:1.79||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:DRS1(YLL008W)|FD-Score:-4.64|P-value:1.75E-6|Clearance:0||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:ERG11(YHR007C)|FD-Score:4.29|P-value:9.08E-6|Clearance:0.24||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GLC7(YER133W)|FD-Score:-3.33|P-value:4.40E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI14(YJR013W)|FD-Score:4.96|P-value:3.51E-7|Clearance:0.12||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:HRR25(YPL204W)|FD-Score:-3.5|P-value:2.29E-4|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:KOG1(YHR186C)|FD-Score:11.9|P-value:7.61E-33|Clearance:4.37||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:PKC1(YBL105C)|FD-Score:4.31|P-value:8.07E-6|Clearance:0.03||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RPA43(YOR340C)|FD-Score:4.05|P-value:2.56E-5|Clearance:0.16||SGD DESC:RNA polymerase I subunit A43 Gene:RPB8(YOR224C)|FD-Score:3.21|P-value:6.56E-4|Clearance:0.05||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC82(YPR190C)|FD-Score:3.43|P-value:3.05E-4|Clearance:0.16||SGD DESC:RNA polymerase III subunit C82 Gene:RPL25(YOL127W)|FD-Score:4.84|P-value:6.51E-7|Clearance:0.36||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RSM10(YDR041W)|FD-Score:5.72|P-value:5.35E-9|Clearance:0.39||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins Gene:SPC97(YHR172W)|FD-Score:3.26|P-value:5.51E-4|Clearance:0.02||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SUI2(YJR007W)|FD-Score:5.33|P-value:4.90E-8|Clearance:0.29||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TAF5(YBR198C)|FD-Score:3.13|P-value:8.77E-4|Clearance:0.08||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TSC11(YER093C)|FD-Score:3.89|P-value:4.99E-5|Clearance:0.09||SGD DESC:Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain Gene:TUB1(YML085C)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.17||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UGP1(YKL035W)|FD-Score:-4.14|P-value:1.77E-5|Clearance:0||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:YJL086C(YJL086C_d)|FD-Score:5.04|P-value:2.28E-7|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR230W(YLR230W_d)|FD-Score:-4.62|P-value:1.91E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:YOR146W(YOR146W_d)|FD-Score:3.8|P-value:7.16E-5|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPL238C(YPL238C_d)|FD-Score:3.16|P-value:7.90E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 312145
Download HOP data (tab-delimited text)  (excel)
Gene:ADE6(YGR061C)|FD-Score:5.74|P-value:4.84E-9||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ADH4(YGL256W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:APL5(YPL195W)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APL6(YGR261C)|FD-Score:5.55|P-value:1.44E-8||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:APM3(YBR288C)|FD-Score:4.48|P-value:3.76E-6||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:APS3(YJL024C)|FD-Score:4.87|P-value:5.65E-7||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:ARF1(YDL192W)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:BCK1(YJL095W)|FD-Score:10.4|P-value:2.00E-25||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CCZ1(YBR131W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway Gene:CDC50(YCR094W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CKA2(YOR061W)|FD-Score:6.73|P-value:8.22E-12||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CSF1(YLR087C)|FD-Score:12.5|P-value:6.28E-36||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CTP1(YBR291C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:CUR1(YPR158W)|FD-Score:3.28|P-value:5.10E-4||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:CYB5(YNL111C)|FD-Score:6.52|P-value:3.42E-11||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DST1(YGL043W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ERG2(YMR202W)|FD-Score:12.4|P-value:1.19E-35||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:4.63|P-value:1.83E-6||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERG5(YMR015C)|FD-Score:4.86|P-value:5.94E-7||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FYV10(YIL097W)|FD-Score:3.12|P-value:8.89E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GAL11(YOL051W)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCV2(YMR189W)|FD-Score:4.34|P-value:7.10E-6||SGD DESC:P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm Gene:GET3(YDL100C)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GLE2(YER107C)|FD-Score:6.22|P-value:2.41E-10||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GTR1(YML121W)|FD-Score:15.2|P-value:1.62E-52||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GTR2(YGR163W)|FD-Score:8.43|P-value:1.72E-17||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HFI1(YPL254W)|FD-Score:5.81|P-value:3.09E-9||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HTD2(YHR067W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:HXT12(YIL170W)|FD-Score:4.92|P-value:4.24E-7||SGD DESC:Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family Gene:IRC4(YDR540C)|FD-Score:4.3|P-value:8.61E-6||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRE1(YHR079C)|FD-Score:8.19|P-value:1.30E-16||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:JJJ2(YJL162C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein Gene:KIN82(YCR091W)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p Gene:LEM3(YNL323W)|FD-Score:5.28|P-value:6.52E-8||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LIP5(YOR196C)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LPD1(YFL018C)|FD-Score:6.06|P-value:6.76E-10||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:LRG1(YDL240W)|FD-Score:-5.36|P-value:4.20E-8||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:LSM1(YJL124C)|FD-Score:5.46|P-value:2.36E-8||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LSM6(YDR378C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:LSM7(YNL147W)|FD-Score:6.89|P-value:2.84E-12||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:LYS12(YIL094C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:MCT1(YOR221C)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDM20(YOL076W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MED1(YPR070W)|FD-Score:5.01|P-value:2.69E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MEH1(YKR007W)|FD-Score:7.68|P-value:7.72E-15||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MIG1(YGL035C)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion Gene:MIR1(YJR077C)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MOD5(YOR274W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MOG1(YJR074W)|FD-Score:3.84|P-value:6.24E-5||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MON1(YGL124C)|FD-Score:4.76|P-value:9.84E-7||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MTC6(YHR151C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:NAT1(YDL040C)|FD-Score:6.61|P-value:1.98E-11||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NMD4(YLR363C)|FD-Score:6.02|P-value:8.97E-10||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NPR3(YHL023C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:NUP170(YBL079W)|FD-Score:15.1|P-value:1.31E-51||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:NUP188(YML103C)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188 Gene:OCA2(YNL056W)|FD-Score:4.48|P-value:3.77E-6||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OCA4(YCR095C_p)|FD-Score:3.85|P-value:5.79E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OCA6(YDR067C_p)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PCA1(YBR295W)|FD-Score:4.55|P-value:2.66E-6||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PDR1(YGL013C)|FD-Score:14.8|P-value:7.26E-50||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEP12(YOR036W)|FD-Score:4.78|P-value:8.63E-7||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:6.54|P-value:2.99E-11||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:4.93|P-value:4.01E-7||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX3(YDR329C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PGD1(YGL025C)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO80(YOL001W)|FD-Score:6.42|P-value:6.86E-11||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PHO84(YML123C)|FD-Score:4.84|P-value:6.45E-7||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PIN4(YBL051C)|FD-Score:10.6|P-value:2.54E-26||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PRO2(YOR323C)|FD-Score:4.43|P-value:4.76E-6||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRS3(YHL011C)|FD-Score:9.47|P-value:1.42E-21||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PRS5(YOL061W)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress Gene:PTR2(YKR093W)|FD-Score:5.69|P-value:6.39E-9||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:QRI5(YLR204W)|FD-Score:7.55|P-value:2.23E-14||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:REF2(YDR195W)|FD-Score:7.21|P-value:2.70E-13||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RIM101(YHL027W)|FD-Score:3.89|P-value:5.02E-5||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RPE1(YJL121C)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL34B(YIL052C)|FD-Score:8.27|P-value:6.43E-17||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:4|P-value:3.17E-5||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RTG1(YOL067C)|FD-Score:6.89|P-value:2.86E-12||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTG2(YGL252C)|FD-Score:6.62|P-value:1.85E-11||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:RTG3(YBL103C)|FD-Score:3.95|P-value:3.88E-5||SGD DESC:Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways Gene:RTT10(YPL183C)|FD-Score:3.8|P-value:7.27E-5||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAC1(YKL212W)|FD-Score:6.38|P-value:8.64E-11||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SEC22(YLR268W)|FD-Score:7.41|P-value:6.15E-14||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SET4(YJL105W)|FD-Score:4.58|P-value:2.35E-6||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SIW14(YNL032W)|FD-Score:7.24|P-value:2.21E-13||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SLM1(YIL105C)|FD-Score:8.74|P-value:1.13E-18||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication Gene:SLM4(YBR077C)|FD-Score:10.6|P-value:1.80E-26||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SLT2(YHR030C)|FD-Score:6.93|P-value:2.11E-12||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SLZ1(YNL196C_p)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNF7(YLR025W)|FD-Score:5.51|P-value:1.77E-8||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:5.25|P-value:7.66E-8||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPT3(YDR392W)|FD-Score:9.86|P-value:3.02E-23||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT7(YBR081C)|FD-Score:5.06|P-value:2.10E-7||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SPT8(YLR055C)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SRB8(YCR081W)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SRN2(YLR119W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SSN2(YDR443C)|FD-Score:7.44|P-value:4.85E-14||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SSN3(YPL042C)|FD-Score:11|P-value:2.18E-28||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SSN8(YNL025C)|FD-Score:3.88|P-value:5.33E-5||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:SSZ1(YHR064C)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:STP22(YCL008C)|FD-Score:4.35|P-value:6.78E-6||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUR4(YLR372W)|FD-Score:5.92|P-value:1.58E-9||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TCO89(YPL180W)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:TOM70(YNL121C)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TOR1(YJR066W)|FD-Score:5.59|P-value:1.13E-8||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:TPK1(YJL164C)|FD-Score:5.27|P-value:6.80E-8||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TRM1(YDR120C)|FD-Score:6.78|P-value:6.13E-12||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TRM7(YBR061C)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:UBP3(YER151C)|FD-Score:5.38|P-value:3.73E-8||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:URC2(YDR520C_p)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:URE2(YNL229C)|FD-Score:9.95|P-value:1.23E-23||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VAM3(YOR106W)|FD-Score:4.61|P-value:2.03E-6||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VAM6(YDL077C)|FD-Score:9.15|P-value:2.84E-20||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VAM7(YGL212W)|FD-Score:11.4|P-value:1.28E-30||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VIK1(YPL253C)|FD-Score:5.24|P-value:7.93E-8||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:VPS3(YDR495C)|FD-Score:7.35|P-value:9.92E-14||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS4(YPR173C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS41(YDR080W)|FD-Score:10.2|P-value:1.35E-24||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:VPS45(YGL095C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS61(YDR136C_d)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS8(YAL002W)|FD-Score:4.49|P-value:3.58E-6||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:VPS9(YML097C)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YAL042C-A(YAL042C-A_d)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YAP6(YDR259C)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication Gene:YBR064W(YBR064W_d)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YBR206W(YBR206W_d)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YCR087C-A(YCR087C-A_p)|FD-Score:10.4|P-value:9.13E-26||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDL119C(YDL119C_p)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YER119C-A(YER119C-A_d)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGR164W(YGR164W_d)|FD-Score:7.01|P-value:1.17E-12||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL055C(YIL055C_p)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Putative protein of unknown function Gene:YJR018W(YJR018W_d)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLL007C(YLL007C_p)|FD-Score:-3.13|P-value:8.77E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YML007C-A(YML007C-A_p)|FD-Score:4.53|P-value:2.91E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML094C-A(YML094C-A_d)|FD-Score:5.35|P-value:4.51E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YML122C(YML122C_d)|FD-Score:7.48|P-value:3.72E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR031W-A(YMR031W-A_d)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YNL190W(YNL190W_p)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YNL235C(YNL235C_d)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YNL276C(YNL276C_d)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YOL050C(YOL050C_d)|FD-Score:9.83|P-value:4.23E-23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YPK1(YKL126W)|FD-Score:10.2|P-value:8.38E-25||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL068C(YPL068C_p)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR064W(YPR064W_d)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR114W(YPR114W_p)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Putative protein of unknown function Gene:YPT7(YML001W)|FD-Score:12.3|P-value:2.73E-35||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 Gene:YTP1(YNL237W)|FD-Score:6.03|P-value:8.12E-10||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ZUO1(YGR285C)|FD-Score:3.81|P-value:6.87E-5||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p Gene:ADE6(YGR061C)|FD-Score:5.74|P-value:4.84E-9||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ADH4(YGL256W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:APL5(YPL195W)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APL6(YGR261C)|FD-Score:5.55|P-value:1.44E-8||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:APM3(YBR288C)|FD-Score:4.48|P-value:3.76E-6||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:APS3(YJL024C)|FD-Score:4.87|P-value:5.65E-7||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:ARF1(YDL192W)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:BCK1(YJL095W)|FD-Score:10.4|P-value:2.00E-25||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CCZ1(YBR131W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway Gene:CDC50(YCR094W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CKA2(YOR061W)|FD-Score:6.73|P-value:8.22E-12||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CSF1(YLR087C)|FD-Score:12.5|P-value:6.28E-36||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CTP1(YBR291C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:CUR1(YPR158W)|FD-Score:3.28|P-value:5.10E-4||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:CYB5(YNL111C)|FD-Score:6.52|P-value:3.42E-11||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DST1(YGL043W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ERG2(YMR202W)|FD-Score:12.4|P-value:1.19E-35||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:4.63|P-value:1.83E-6||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERG5(YMR015C)|FD-Score:4.86|P-value:5.94E-7||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FYV10(YIL097W)|FD-Score:3.12|P-value:8.89E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GAL11(YOL051W)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCV2(YMR189W)|FD-Score:4.34|P-value:7.10E-6||SGD DESC:P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm Gene:GET3(YDL100C)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GLE2(YER107C)|FD-Score:6.22|P-value:2.41E-10||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GTR1(YML121W)|FD-Score:15.2|P-value:1.62E-52||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GTR2(YGR163W)|FD-Score:8.43|P-value:1.72E-17||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HFI1(YPL254W)|FD-Score:5.81|P-value:3.09E-9||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HTD2(YHR067W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:HXT12(YIL170W)|FD-Score:4.92|P-value:4.24E-7||SGD DESC:Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family Gene:IRC4(YDR540C)|FD-Score:4.3|P-value:8.61E-6||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRE1(YHR079C)|FD-Score:8.19|P-value:1.30E-16||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:JJJ2(YJL162C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein Gene:KIN82(YCR091W)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p Gene:LEM3(YNL323W)|FD-Score:5.28|P-value:6.52E-8||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LIP5(YOR196C)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LPD1(YFL018C)|FD-Score:6.06|P-value:6.76E-10||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:LRG1(YDL240W)|FD-Score:-5.36|P-value:4.20E-8||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:LSM1(YJL124C)|FD-Score:5.46|P-value:2.36E-8||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LSM6(YDR378C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:LSM7(YNL147W)|FD-Score:6.89|P-value:2.84E-12||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:LYS12(YIL094C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:MCT1(YOR221C)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDM20(YOL076W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MED1(YPR070W)|FD-Score:5.01|P-value:2.69E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MEH1(YKR007W)|FD-Score:7.68|P-value:7.72E-15||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MIG1(YGL035C)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion Gene:MIR1(YJR077C)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MOD5(YOR274W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MOG1(YJR074W)|FD-Score:3.84|P-value:6.24E-5||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MON1(YGL124C)|FD-Score:4.76|P-value:9.84E-7||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MTC6(YHR151C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:NAT1(YDL040C)|FD-Score:6.61|P-value:1.98E-11||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NMD4(YLR363C)|FD-Score:6.02|P-value:8.97E-10||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NPR3(YHL023C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:NUP170(YBL079W)|FD-Score:15.1|P-value:1.31E-51||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:NUP188(YML103C)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188 Gene:OCA2(YNL056W)|FD-Score:4.48|P-value:3.77E-6||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OCA4(YCR095C_p)|FD-Score:3.85|P-value:5.79E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OCA6(YDR067C_p)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PCA1(YBR295W)|FD-Score:4.55|P-value:2.66E-6||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PDR1(YGL013C)|FD-Score:14.8|P-value:7.26E-50||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEP12(YOR036W)|FD-Score:4.78|P-value:8.63E-7||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:6.54|P-value:2.99E-11||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:4.93|P-value:4.01E-7||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX3(YDR329C)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PGD1(YGL025C)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO80(YOL001W)|FD-Score:6.42|P-value:6.86E-11||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PHO84(YML123C)|FD-Score:4.84|P-value:6.45E-7||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PIN4(YBL051C)|FD-Score:10.6|P-value:2.54E-26||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PRO2(YOR323C)|FD-Score:4.43|P-value:4.76E-6||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRS3(YHL011C)|FD-Score:9.47|P-value:1.42E-21||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PRS5(YOL061W)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress Gene:PTR2(YKR093W)|FD-Score:5.69|P-value:6.39E-9||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:QRI5(YLR204W)|FD-Score:7.55|P-value:2.23E-14||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:REF2(YDR195W)|FD-Score:7.21|P-value:2.70E-13||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RIM101(YHL027W)|FD-Score:3.89|P-value:5.02E-5||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RPE1(YJL121C)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL34B(YIL052C)|FD-Score:8.27|P-value:6.43E-17||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:4|P-value:3.17E-5||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RTG1(YOL067C)|FD-Score:6.89|P-value:2.86E-12||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTG2(YGL252C)|FD-Score:6.62|P-value:1.85E-11||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:RTG3(YBL103C)|FD-Score:3.95|P-value:3.88E-5||SGD DESC:Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways Gene:RTT10(YPL183C)|FD-Score:3.8|P-value:7.27E-5||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAC1(YKL212W)|FD-Score:6.38|P-value:8.64E-11||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SEC22(YLR268W)|FD-Score:7.41|P-value:6.15E-14||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SET4(YJL105W)|FD-Score:4.58|P-value:2.35E-6||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SIW14(YNL032W)|FD-Score:7.24|P-value:2.21E-13||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SLM1(YIL105C)|FD-Score:8.74|P-value:1.13E-18||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication Gene:SLM4(YBR077C)|FD-Score:10.6|P-value:1.80E-26||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SLT2(YHR030C)|FD-Score:6.93|P-value:2.11E-12||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SLZ1(YNL196C_p)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNF7(YLR025W)|FD-Score:5.51|P-value:1.77E-8||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:5.25|P-value:7.66E-8||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPT3(YDR392W)|FD-Score:9.86|P-value:3.02E-23||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT7(YBR081C)|FD-Score:5.06|P-value:2.10E-7||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SPT8(YLR055C)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SRB8(YCR081W)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SRN2(YLR119W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SSN2(YDR443C)|FD-Score:7.44|P-value:4.85E-14||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SSN3(YPL042C)|FD-Score:11|P-value:2.18E-28||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SSN8(YNL025C)|FD-Score:3.88|P-value:5.33E-5||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:SSZ1(YHR064C)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:STP22(YCL008C)|FD-Score:4.35|P-value:6.78E-6||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUR4(YLR372W)|FD-Score:5.92|P-value:1.58E-9||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TCO89(YPL180W)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:TOM70(YNL121C)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TOR1(YJR066W)|FD-Score:5.59|P-value:1.13E-8||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:TPK1(YJL164C)|FD-Score:5.27|P-value:6.80E-8||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TRM1(YDR120C)|FD-Score:6.78|P-value:6.13E-12||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TRM7(YBR061C)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:UBP3(YER151C)|FD-Score:5.38|P-value:3.73E-8||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:URC2(YDR520C_p)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:URE2(YNL229C)|FD-Score:9.95|P-value:1.23E-23||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VAM3(YOR106W)|FD-Score:4.61|P-value:2.03E-6||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VAM6(YDL077C)|FD-Score:9.15|P-value:2.84E-20||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VAM7(YGL212W)|FD-Score:11.4|P-value:1.28E-30||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VIK1(YPL253C)|FD-Score:5.24|P-value:7.93E-8||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:VPS3(YDR495C)|FD-Score:7.35|P-value:9.92E-14||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS4(YPR173C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS41(YDR080W)|FD-Score:10.2|P-value:1.35E-24||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:VPS45(YGL095C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS61(YDR136C_d)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS8(YAL002W)|FD-Score:4.49|P-value:3.58E-6||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:VPS9(YML097C)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YAL042C-A(YAL042C-A_d)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YAP6(YDR259C)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication Gene:YBR064W(YBR064W_d)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YBR206W(YBR206W_d)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YCR087C-A(YCR087C-A_p)|FD-Score:10.4|P-value:9.13E-26||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDL119C(YDL119C_p)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YER119C-A(YER119C-A_d)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGR164W(YGR164W_d)|FD-Score:7.01|P-value:1.17E-12||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL055C(YIL055C_p)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Putative protein of unknown function Gene:YJR018W(YJR018W_d)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLL007C(YLL007C_p)|FD-Score:-3.13|P-value:8.77E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YML007C-A(YML007C-A_p)|FD-Score:4.53|P-value:2.91E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML094C-A(YML094C-A_d)|FD-Score:5.35|P-value:4.51E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YML122C(YML122C_d)|FD-Score:7.48|P-value:3.72E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR031W-A(YMR031W-A_d)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YNL190W(YNL190W_p)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YNL235C(YNL235C_d)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YNL276C(YNL276C_d)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YOL050C(YOL050C_d)|FD-Score:9.83|P-value:4.23E-23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YPK1(YKL126W)|FD-Score:10.2|P-value:8.38E-25||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL068C(YPL068C_p)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR064W(YPR064W_d)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR114W(YPR114W_p)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Putative protein of unknown function Gene:YPT7(YML001W)|FD-Score:12.3|P-value:2.73E-35||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 Gene:YTP1(YNL237W)|FD-Score:6.03|P-value:8.12E-10||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ZUO1(YGR285C)|FD-Score:3.81|P-value:6.87E-5||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YHR186C11.907.61E-334.37KOG1Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
YGL116W7.503.07E-141.79CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YDR041W5.725.35E-90.39RSM10Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
YJR007W5.334.90E-80.29SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YJL086C_d5.042.28E-70.08YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YJR013W4.963.51E-70.12GPI14Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
YOL127W4.846.51E-70.36RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YKL013C4.483.72E-60.17ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YBL105C4.318.07E-60.03PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YHR007C4.299.08E-60.24ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YOR340C4.052.56E-50.16RPA43RNA polymerase I subunit A43
YER093C3.894.99E-50.09TSC11Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain
YOR146W_d3.807.16E-50.09YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YML085C3.721.01E-40.17TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YJR065C3.541.99E-40.12ARP3Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML121W15.201.62E-52GTR1Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
YBL079W15.101.31E-51NUP170Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
YGL013C14.807.26E-50PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YLR087C12.506.28E-36CSF1Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YMR202W12.401.19E-35ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YML001W12.302.73E-35YPT7Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
YGL212W11.401.28E-30VAM7Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
YPL042C11.002.18E-28SSN3Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression
YBR077C10.601.80E-26SLM4Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4
YBL051C10.602.54E-26PIN4Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
YCR087C-A_p10.409.13E-26YCR087C-A_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene
YJL095W10.402.00E-25BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YKL126W10.208.38E-25YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YDR080W10.201.35E-24VPS41Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
YNL229C9.951.23E-23URE2Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion

GO enrichment analysis for SGTC_1877
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4880SGTC_1687st020778 55.4 μMTimTec (Natural product derivative library)42592990.0666667
0.3853.65E-208SGTC_8410251-0215 91.2 μMChemDiv (Drug-like library)38886140.0253165
0.3351.18E-154SGTC_1694st024011 44.9 μMTimTec (Natural product derivative library)27875330.0747664ERG2
0.3131.38E-134SGTC_1873rapamycin 2.0 nMMiscellaneous52846160.100671
0.3048.20E-127SGTC_12870971-0001 34.6 μMChemDiv (Drug-like library)46658690.0322581sphingolipid biosynthesis & PDR1
0.3043.26E-126SGTC_14954469-0029 237.0 μMChemDiv (Drug-like library)7936800.0306122
0.2834.49E-109SGTC_20775212015 200.0 μMChembridge (Fragment library)7809000.0337079
0.2771.99E-104SGTC_23227413319 38.2 μMChembridge (Fragment library)18404000.043956ERAD & cell cycle
0.2716.28E-100SGTC_505gw-5074 63.9 μMICCB bioactive library59242080.0526316
0.2681.61E-97SGTC_2752diclazuril 33.1 μMMiscellaneous4563890.0392157
0.2624.61E-93SGTC_5283492-0019 172.0 μMChemDiv (Drug-like library)28692620.0729167calcium & mitochondrial duress
0.2555.85E-88SGTC_6641222-0054 14.9 μMChemDiv (Drug-like library)68139910.0412371sphingolipid biosynthesis & PDR1
0.2524.53E-86SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.06ERG2
0.2451.10E-81SGTC_8041595-0054 20.9 μMChemDiv (Drug-like library)7615230.0430108sphingolipid biosynthesis & PDR1
0.2433.30E-80SGTC_391gliotoxin 8.3 μMMiscellaneous62230.0891089

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_26373-alpha-acetoxydihydrodeoxygedunin100 μM0.2079216708514Microsource (Natural product library)512.63444.51907
SGTC_2653dihydrofissinolide100 μM0.2018356857746Microsource (Natural product library)514.607223.40808
SGTC_2678equilin100 μM0.1931823247Microsource (Natural product library)268.35023.68812
SGTC_1586deoxycorticosterone acetate53.7 μM0.1914895952TimTec (Pure natural product library)372.497783.56704
SGTC_2412st07713533.4 μM0.18627513258915TimTec (Natural product derivative library)598.55142.194013
SGTC_1630st00520931.3 μM0.1810343367314TimTec (Natural product derivative library)639.904746.46507
SGTC_1609st00157446.4 μM0.183960012TimTec (Natural product derivative library)430.576923.5615
SGTC_1579chlormadinone acetate49.4 μM0.17894745114258Miscellaneous448.979523.70605
SGTC_2650prasterone100 μM0.17647116759247Microsource (Natural product library)288.424423.33812
SGTC_2758dehydroepiandrosterone69.35 μM0.1764715881Miscellaneous288.424423.33812