gemcitabine FDA approved compound

4-amino-1-[(2R,4R,5R)-3,3-difluoro-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidin-2-one

A nucleoside analog which inhibits DNA replication.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Antimetabolites, Antineoplastic;Antiviral Agents;Enzyme Inhibitors;Immunosuppressive Agents;Radiation-Sensitizing Agents



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1879
Screen concentration 100.0 μM
Source Miscellaneous
PubChem CID 60750
SMILES C1=CN(C(=O)N=C1N)C2C(C(C(O2)CO)O)(F)F
Standardized SMILES NC1=NC(=O)N(C=C1)C2OC(CO)C(O)C2(F)F
Molecular weight 263.1981
ALogP -1.39
H-bond donor count 3
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.74
% growth inhibition (Hom. pool) -1.27


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 60750
Download HIP data (tab-delimited text)  (excel)
Gene:BET4(YJL031C)|FD-Score:-3.35|P-value:3.98E-4|Clearance:0||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:FRQ1(YDR373W)|FD-Score:-3.72|P-value:9.84E-5|Clearance:0||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:PFY1(YOR122C)|FD-Score:3.56|P-value:1.83E-4|Clearance:0.12||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:RPL10(YLR075W)|FD-Score:4.4|P-value:5.48E-6|Clearance:0.83||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPT1(YKL145W)|FD-Score:3.13|P-value:8.85E-4|Clearance:0.05||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:SUI2(YJR007W)|FD-Score:3.44|P-value:2.88E-4|Clearance:0.32||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:YPR136C(YPR136C_d)|FD-Score:7.03|P-value:9.97E-13|Clearance:2.64||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:BET4(YJL031C)|FD-Score:-3.35|P-value:3.98E-4|Clearance:0||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:FRQ1(YDR373W)|FD-Score:-3.72|P-value:9.84E-5|Clearance:0||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:PFY1(YOR122C)|FD-Score:3.56|P-value:1.83E-4|Clearance:0.12||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:RPL10(YLR075W)|FD-Score:4.4|P-value:5.48E-6|Clearance:0.83||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPT1(YKL145W)|FD-Score:3.13|P-value:8.85E-4|Clearance:0.05||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:SUI2(YJR007W)|FD-Score:3.44|P-value:2.88E-4|Clearance:0.32||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:YPR136C(YPR136C_d)|FD-Score:7.03|P-value:9.97E-13|Clearance:2.64||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 60750
Download HOP data (tab-delimited text)  (excel)
Gene:APP1(YNL094W)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ATG5(YPL149W)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:ATS1(YAL020C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BNI4(YNL233W)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BUD4(YJR092W)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Anillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p Gene:CAF120(YNL278W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:CAF20(YOR276W)|FD-Score:-3.09|P-value:9.99E-4||SGD DESC:Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E Gene:CDC55(YGL190C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CIN1(YOR349W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:DCV1(YFR012W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication Gene:DOM34(YNL001W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication Gene:DPB3(YBR278W)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:DUN1(YDL101C)|FD-Score:4.47|P-value:4.00E-6||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:ECM10(YEL030W)|FD-Score:-3.72|P-value:9.97E-5||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:ECM3(YOR092W)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication Gene:EKI1(YDR147W)|FD-Score:4.51|P-value:3.21E-6||SGD DESC:Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication Gene:ERG3(YLR056W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:GCN4(YEL009C)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GRX6(YDL010W)|FD-Score:-6.11|P-value:4.87E-10||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:GYP6(YJL044C)|FD-Score:5.84|P-value:2.55E-9||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HIS1(YER055C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HMO1(YDR174W)|FD-Score:3.76|P-value:8.47E-5||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HRQ1(YDR291W_p)|FD-Score:-4.93|P-value:4.08E-7||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:HSL7(YBR133C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HUA1(YGR268C)|FD-Score:4.38|P-value:5.94E-6||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IRC14(YOR135C_d)|FD-Score:-3.86|P-value:5.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:JJJ2(YJL162C)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein Gene:KES1(YPL145C)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:MAE1(YKL029C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MAP1(YLR244C)|FD-Score:-4.12|P-value:1.92E-5||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MIC14(YDR031W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MNL2(YLR057W)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MRK1(YDL079C)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MSC3(YLR219W)|FD-Score:-3.73|P-value:9.43E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:NCE102(YPR149W)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) Gene:PAU11(YGL261C_p)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PCD1(YLR151C)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PDR16(YNL231C)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PEX22(YAL055W)|FD-Score:4.87|P-value:5.60E-7||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PIH1(YHR034C)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PNG1(YPL096W)|FD-Score:3.82|P-value:6.80E-5||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PPR1(YLR014C)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p Gene:RPL19A(YBR084C-A)|FD-Score:-5.68|P-value:6.86E-9||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPS14A(YCR031C)|FD-Score:3.19|P-value:6.99E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RRI1(YDL216C)|FD-Score:4.02|P-value:2.90E-5||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:RRT12(YCR045C)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RTT103(YDR289C)|FD-Score:-3.83|P-value:6.50E-5||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SDH4(YDR178W)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SET4(YJL105W)|FD-Score:4.97|P-value:3.30E-7||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SHE1(YBL031W)|FD-Score:4.45|P-value:4.23E-6||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SNO1(YMR095C)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase Gene:SPT21(YMR179W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SUT2(YPR009W)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p Gene:TEL1(YBL088C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:UTH1(YKR042W)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:VPS73(YGL104C)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YBL053W(YBL053W_d)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR064W(YBR064W_d)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YCR007C(YCR007C_p)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YCR050C(YCR050C_p)|FD-Score:4.32|P-value:7.74E-6||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YFL015C(YFL015C_d)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YGR066C(YGR066C_p)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Putative protein of unknown function Gene:YGR125W(YGR125W_p)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHR140W(YHR140W_p)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Putative integral membrane protein of unknown function Gene:YJL169W(YJL169W_d)|FD-Score:-3.15|P-value:8.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YJR039W(YJR039W_p)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKR078W(YKR078W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YLR290C(YLR290C_p)|FD-Score:-3.98|P-value:3.49E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene Gene:YMR279C(YMR279C)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YMR316C-A(YMR316C-A_p)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YNL024C(YNL024C_p)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Putative methyltransferase; has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; despite similarity to methyltransferases, null mutant does not display alterations in lysine methylation pattern Gene:YNL276C(YNL276C_d)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YNL285W(YNL285W_d)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL097W-A(YOL097W-A_p)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOL118C(YOL118C_d)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL131W(YOL131W_p)|FD-Score:3.73|P-value:9.73E-5||SGD DESC:Putative protein of unknown function Gene:YOL153C(YOL153C)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Hypothetical protein Gene:YOR268C(YOR268C_p)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Putative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene Gene:YPR109W(YPR109W_p)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:YPR130C(YPR130C_d)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:APP1(YNL094W)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ATG5(YPL149W)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:ATS1(YAL020C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BNI4(YNL233W)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BUD4(YJR092W)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Anillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p Gene:CAF120(YNL278W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:CAF20(YOR276W)|FD-Score:-3.09|P-value:9.99E-4||SGD DESC:Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E Gene:CDC55(YGL190C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CIN1(YOR349W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:DCV1(YFR012W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication Gene:DOM34(YNL001W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication Gene:DPB3(YBR278W)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:DUN1(YDL101C)|FD-Score:4.47|P-value:4.00E-6||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:ECM10(YEL030W)|FD-Score:-3.72|P-value:9.97E-5||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:ECM3(YOR092W)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication Gene:EKI1(YDR147W)|FD-Score:4.51|P-value:3.21E-6||SGD DESC:Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication Gene:ERG3(YLR056W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:GCN4(YEL009C)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GRX6(YDL010W)|FD-Score:-6.11|P-value:4.87E-10||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:GYP6(YJL044C)|FD-Score:5.84|P-value:2.55E-9||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HIS1(YER055C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HMO1(YDR174W)|FD-Score:3.76|P-value:8.47E-5||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HRQ1(YDR291W_p)|FD-Score:-4.93|P-value:4.08E-7||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:HSL7(YBR133C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HUA1(YGR268C)|FD-Score:4.38|P-value:5.94E-6||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IRC14(YOR135C_d)|FD-Score:-3.86|P-value:5.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:JJJ2(YJL162C)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein Gene:KES1(YPL145C)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:MAE1(YKL029C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MAP1(YLR244C)|FD-Score:-4.12|P-value:1.92E-5||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MIC14(YDR031W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MNL2(YLR057W)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MRK1(YDL079C)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MSC3(YLR219W)|FD-Score:-3.73|P-value:9.43E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:NCE102(YPR149W)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) Gene:PAU11(YGL261C_p)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PCD1(YLR151C)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PDR16(YNL231C)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PEX22(YAL055W)|FD-Score:4.87|P-value:5.60E-7||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PIH1(YHR034C)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PNG1(YPL096W)|FD-Score:3.82|P-value:6.80E-5||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PPR1(YLR014C)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p Gene:RPL19A(YBR084C-A)|FD-Score:-5.68|P-value:6.86E-9||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPS14A(YCR031C)|FD-Score:3.19|P-value:6.99E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RRI1(YDL216C)|FD-Score:4.02|P-value:2.90E-5||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:RRT12(YCR045C)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RTT103(YDR289C)|FD-Score:-3.83|P-value:6.50E-5||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SDH4(YDR178W)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SET4(YJL105W)|FD-Score:4.97|P-value:3.30E-7||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SHE1(YBL031W)|FD-Score:4.45|P-value:4.23E-6||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SNO1(YMR095C)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase Gene:SPT21(YMR179W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SUT2(YPR009W)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p Gene:TEL1(YBL088C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:UTH1(YKR042W)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:VPS73(YGL104C)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YBL053W(YBL053W_d)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR064W(YBR064W_d)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YCR007C(YCR007C_p)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YCR050C(YCR050C_p)|FD-Score:4.32|P-value:7.74E-6||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YFL015C(YFL015C_d)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YGR066C(YGR066C_p)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Putative protein of unknown function Gene:YGR125W(YGR125W_p)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHR140W(YHR140W_p)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Putative integral membrane protein of unknown function Gene:YJL169W(YJL169W_d)|FD-Score:-3.15|P-value:8.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YJR039W(YJR039W_p)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKR078W(YKR078W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YLR290C(YLR290C_p)|FD-Score:-3.98|P-value:3.49E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene Gene:YMR279C(YMR279C)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YMR316C-A(YMR316C-A_p)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YNL024C(YNL024C_p)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Putative methyltransferase; has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; despite similarity to methyltransferases, null mutant does not display alterations in lysine methylation pattern Gene:YNL276C(YNL276C_d)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YNL285W(YNL285W_d)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL097W-A(YOL097W-A_p)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOL118C(YOL118C_d)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL131W(YOL131W_p)|FD-Score:3.73|P-value:9.73E-5||SGD DESC:Putative protein of unknown function Gene:YOL153C(YOL153C)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Hypothetical protein Gene:YOR268C(YOR268C_p)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Putative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene Gene:YPR109W(YPR109W_p)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:YPR130C(YPR130C_d)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR136C_d7.039.97E-132.64YPR136C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
YLR075W4.405.48E-60.83RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YOR122C3.561.83E-40.12PFY1Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress
YJR007W3.442.88E-40.32SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YKL145W3.138.85E-40.05RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YDL209C3.080.001050.02CWC2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
YPR175W3.050.001130.10DPB2Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate
YPR186C2.950.001580.04PZF1Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
YFL039C2.920.001770.17ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YML093W2.750.003010.02UTP14Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YDL105W2.730.003180.02NSE4Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YGL097W2.700.003420.05SRM1Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p
YKL195W2.660.003940.04MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YLR186W2.620.004380.00EMG1Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity
YOR257W2.620.004410.07CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL044C5.842.55E-9GYP6GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport
YJL105W4.973.30E-7SET4Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication
YAL055W4.875.60E-7PEX22Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation
YGR066C_p4.651.69E-6YGR066C_pPutative protein of unknown function
YDR147W4.513.21E-6EKI1Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication
YDL101C4.474.00E-6DUN1Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair
YBL031W4.454.23E-6SHE1Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function
YGR268C4.385.94E-6HUA1Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
YCR050C_p4.327.74E-6YCR050C_pNon-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein
YBR278W4.022.86E-5DPB3Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication
YDL216C4.022.90E-5RRI1Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling
YDR178W4.013.02E-5SDH4Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain
YOL153C3.993.34E-5YOL153CHypothetical protein
YPR109W_p3.963.73E-5YPR109W_pPredicted membrane protein; dipoid deletion strain has high budding index
YPR149W3.963.76E-5NCE102Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs)

GO enrichment analysis for SGTC_1879
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1271.39E-22SGTC_28749043904 71.4 μMChembridge (Drug-like library)44634280.0684932
0.0832.06E-10SGTC_1867st057372 64.0 μMTimTec (Natural product derivative library)6888370.0714286
0.0772.53E-9SGTC_7131488-1073 324.0 μMChemDiv (Drug-like library)33125420.0506329ERG2
0.0691.21E-7SGTC_1838st055369 84.5 μMTimTec (Natural product derivative library)6760360.0746269
0.0691.31E-7SGTC_1852st056254 31.4 μMTimTec (Natural product derivative library)4425830.0684932
0.0663.65E-7SGTC_28789043973 26.0 μMChembridge (Drug-like library)64651670.0512821
0.0621.54E-6SGTC_2652actinonin 100.0 μMMicrosource (Natural product library)4436000.0813954
0.0622.19E-6SGTC_1957st077035 62.4 μMTimTec (Natural product derivative library)7055930.0617284
0.0612.79E-6SGTC_7083448-5400 146.0 μMChemDiv (Drug-like library)34621950.0714286
0.0587.78E-6SGTC_22907904846 200.0 μMChembridge (Fragment library)29621540.101449
0.0571.06E-5SGTC_1984st072354 53.1 μMTimTec (Natural product derivative library)57629380.0888889
0.0571.39E-5SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.0729167cell wall signaling
0.0543.00E-5SGTC_2654protoveratrine a 100.0 μMMicrosource (Natural product library)57022780.0666667
0.0543.58E-5SGTC_1916st067606 49.2 μMTimTec (Natural product derivative library)195902790.0612245
0.0534.25E-5SGTC_1873rapamycin 2.0 nMMiscellaneous52846160.0833333

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1536cytidine-2',3'-monophosphoric acid; 3'-cytidylic acid61.9 μM0.566535TimTec (Pure natural product library)323.196522-2.46858
SGTC_1690st02350241.2 μM0.34782624207482TimTec (Natural product derivative library)485.721866.56126calcium & mitochondrial duress
SGTC_2615-fluorouridine15.38 μM0.2786891821Miscellaneous262.191843-2.54147exosome
SGTC_1540guanosine70.6 μM0.2686576802TimTec (Pure natural product library)283.24072-2.3856
SGTC_15346-azauridine81.6 μM0.262295233502TimTec (Pure natural product library)245.18944-2.43247RNA pol III & RNase P/MRP
SGTC_1528arabinosylhypoxanthine74.6 μM0.220588804TimTec (Pure natural product library)268.22608-2.24946
SGTC_1956st07708554 μM0.224208685TimTec (Natural product derivative library)370.30812-0.531510
SGTC_1789tunicamycin25 nM0.19387823847177ICCB bioactive library718.70344-4.5341116unfolded protein response
SGTC_255tunicamycin200.47 nM0.19387823847177ICCB bioactive library718.70344-4.5341116unfolded protein response
SGTC_2410aloe-emodin-8-o-glycoside46.3 μM0.17948714077415TimTec (Pure natural product library)432.3775-0.21610mitochondrial processes
SGTC_1569carminic acid40.6 μM0.17721514749TimTec (Pure natural product library)492.3864-0.436913