3229-0966

3-[(Z)-(1,3-benzothiazol-2-ylhydrazinylidene)methyl]phenol

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_188
Screen concentration 278.5 μM
Source ChemDiv (Drug-like library)
PubChem CID 5722370
SMILES C1=CC=C2C(=C1)N=C(S2)NN=CC3=CC(=CC=C3)O
Standardized SMILES Oc1cccc(C=NNc2nc3ccccc3s2)c1
Molecular weight 269.3216
ALogP 3.83
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 55.07
% growth inhibition (Hom. pool) 42.32


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5722370
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:5.15|P-value:1.27E-7|Clearance:0.11||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP3(YJR065C)|FD-Score:3.27|P-value:5.31E-4|Clearance:0.03||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:BMS1(YPL217C)|FD-Score:3.61|P-value:1.56E-4|Clearance:0.04||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CBK1(YNL161W)|FD-Score:-3.17|P-value:7.54E-4|Clearance:0||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CDC14(YFR028C)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.01||SGD DESC:Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis Gene:CDC45(YLR103C)|FD-Score:-3.14|P-value:8.44E-4|Clearance:0||SGD DESC:DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes Gene:DUT1(YBR252W)|FD-Score:-3.39|P-value:3.52E-4|Clearance:0||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:GLC7(YER133W)|FD-Score:5.04|P-value:2.27E-7|Clearance:0.33||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:KAP95(YLR347C)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.06||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:MIA40(YKL195W)|FD-Score:5.21|P-value:9.65E-8|Clearance:0.05||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NIP1(YMR309C)|FD-Score:3.61|P-value:1.53E-4|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NUP159(YIL115C)|FD-Score:-3.97|P-value:3.57E-5|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:NUP85(YJR042W)|FD-Score:8.3|P-value:5.02E-17|Clearance:1.56||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:PAM16(YJL104W)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.08||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain Gene:PKC1(YBL105C)|FD-Score:6.74|P-value:7.95E-12|Clearance:0.07||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRP24(YMR268C)|FD-Score:5.49|P-value:2.01E-8|Clearance:0.28||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PTR3(YFR029W)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.03||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:RDS3(YPR094W)|FD-Score:3.5|P-value:2.31E-4|Clearance:0.02||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:RLP24(YLR009W)|FD-Score:3.1|P-value:9.63E-4|Clearance:0.01||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPC53(YDL150W)|FD-Score:3.24|P-value:5.98E-4|Clearance:0.05||SGD DESC:RNA polymerase III subunit C53 Gene:RPL18A(YOL120C)|FD-Score:4.3|P-value:8.37E-6|Clearance:0.1||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPO31(YOR116C)|FD-Score:5.16|P-value:1.24E-7|Clearance:0||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPS15(YOL040C)|FD-Score:3.85|P-value:5.97E-5|Clearance:0.1||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS20(YHL015W)|FD-Score:3.18|P-value:7.31E-4|Clearance:0.07||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS5(YJR123W)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.13||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RRP15(YPR143W)|FD-Score:5.79|P-value:3.46E-9|Clearance:0.02||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RSC58(YLR033W)|FD-Score:3.11|P-value:9.21E-4|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEC24(YIL109C)|FD-Score:3.88|P-value:5.18E-5|Clearance:0.03||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SEN54(YPL083C)|FD-Score:-3.15|P-value:8.07E-4|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SPP41(YDR464W)|FD-Score:4.52|P-value:3.09E-6|Clearance:0.09||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:TFC3(YAL001C)|FD-Score:4.2|P-value:1.32E-5|Clearance:0.3||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:THG1(YGR024C)|FD-Score:3.25|P-value:5.85E-4|Clearance:0.01||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:TIF35(YDR429C)|FD-Score:4.43|P-value:4.61E-6|Clearance:0.13||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM17(YJL143W)|FD-Score:4.72|P-value:1.20E-6|Clearance:0.2||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TOM40(YMR203W)|FD-Score:5.85|P-value:2.47E-9|Clearance:0.06||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TSC10(YBR265W)|FD-Score:3.75|P-value:8.89E-5|Clearance:0.11||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:YBR190W(YBR190W_d)|FD-Score:5.78|P-value:3.84E-9|Clearance:0.29||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YGR265W(YGR265W_d)|FD-Score:6.67|P-value:1.26E-11|Clearance:0.82||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YMR290W-A(YMR290W-A_d)|FD-Score:8.65|P-value:2.69E-18|Clearance:1.56||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YTM1(YOR272W)|FD-Score:3.9|P-value:4.75E-5|Clearance:0.02||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats Gene:ACC1(YNR016C)|FD-Score:5.15|P-value:1.27E-7|Clearance:0.11||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP3(YJR065C)|FD-Score:3.27|P-value:5.31E-4|Clearance:0.03||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:BMS1(YPL217C)|FD-Score:3.61|P-value:1.56E-4|Clearance:0.04||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CBK1(YNL161W)|FD-Score:-3.17|P-value:7.54E-4|Clearance:0||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CDC14(YFR028C)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.01||SGD DESC:Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis Gene:CDC45(YLR103C)|FD-Score:-3.14|P-value:8.44E-4|Clearance:0||SGD DESC:DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes Gene:DUT1(YBR252W)|FD-Score:-3.39|P-value:3.52E-4|Clearance:0||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:GLC7(YER133W)|FD-Score:5.04|P-value:2.27E-7|Clearance:0.33||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:KAP95(YLR347C)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.06||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:MIA40(YKL195W)|FD-Score:5.21|P-value:9.65E-8|Clearance:0.05||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NIP1(YMR309C)|FD-Score:3.61|P-value:1.53E-4|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NUP159(YIL115C)|FD-Score:-3.97|P-value:3.57E-5|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:NUP85(YJR042W)|FD-Score:8.3|P-value:5.02E-17|Clearance:1.56||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:PAM16(YJL104W)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.08||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain Gene:PKC1(YBL105C)|FD-Score:6.74|P-value:7.95E-12|Clearance:0.07||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRP24(YMR268C)|FD-Score:5.49|P-value:2.01E-8|Clearance:0.28||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PTR3(YFR029W)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.03||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:RDS3(YPR094W)|FD-Score:3.5|P-value:2.31E-4|Clearance:0.02||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:RLP24(YLR009W)|FD-Score:3.1|P-value:9.63E-4|Clearance:0.01||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPC53(YDL150W)|FD-Score:3.24|P-value:5.98E-4|Clearance:0.05||SGD DESC:RNA polymerase III subunit C53 Gene:RPL18A(YOL120C)|FD-Score:4.3|P-value:8.37E-6|Clearance:0.1||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPO31(YOR116C)|FD-Score:5.16|P-value:1.24E-7|Clearance:0||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPS15(YOL040C)|FD-Score:3.85|P-value:5.97E-5|Clearance:0.1||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS20(YHL015W)|FD-Score:3.18|P-value:7.31E-4|Clearance:0.07||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS5(YJR123W)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.13||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RRP15(YPR143W)|FD-Score:5.79|P-value:3.46E-9|Clearance:0.02||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RSC58(YLR033W)|FD-Score:3.11|P-value:9.21E-4|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEC24(YIL109C)|FD-Score:3.88|P-value:5.18E-5|Clearance:0.03||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SEN54(YPL083C)|FD-Score:-3.15|P-value:8.07E-4|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SPP41(YDR464W)|FD-Score:4.52|P-value:3.09E-6|Clearance:0.09||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:TFC3(YAL001C)|FD-Score:4.2|P-value:1.32E-5|Clearance:0.3||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:THG1(YGR024C)|FD-Score:3.25|P-value:5.85E-4|Clearance:0.01||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:TIF35(YDR429C)|FD-Score:4.43|P-value:4.61E-6|Clearance:0.13||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM17(YJL143W)|FD-Score:4.72|P-value:1.20E-6|Clearance:0.2||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TOM40(YMR203W)|FD-Score:5.85|P-value:2.47E-9|Clearance:0.06||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TSC10(YBR265W)|FD-Score:3.75|P-value:8.89E-5|Clearance:0.11||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:YBR190W(YBR190W_d)|FD-Score:5.78|P-value:3.84E-9|Clearance:0.29||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YGR265W(YGR265W_d)|FD-Score:6.67|P-value:1.26E-11|Clearance:0.82||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YMR290W-A(YMR290W-A_d)|FD-Score:8.65|P-value:2.69E-18|Clearance:1.56||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YTM1(YOR272W)|FD-Score:3.9|P-value:4.75E-5|Clearance:0.02||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5722370
Download HOP data (tab-delimited text)  (excel)
Gene:AAH1(YNL141W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome Gene:APS1(YLR170C)|FD-Score:-4.2|P-value:1.34E-5||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ARG82(YDR173C)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARK1(YNL020C)|FD-Score:3.78|P-value:7.99E-5||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ARO80(YDR421W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids Gene:ATG3(YNR007C)|FD-Score:5.64|P-value:8.61E-9||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:ATP10(YLR393W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BCK1(YJL095W)|FD-Score:-6.35|P-value:1.07E-10||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BNI1(YNL271C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BST1(YFL025C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CDC73(YLR418C)|FD-Score:4.4|P-value:5.41E-6||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLB3(YDL155W)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:CLC1(YGR167W)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CNB1(YKL190W)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CPR8(YNR028W)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:CRT10(YOL063C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat Gene:CSF1(YLR087C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DAL81(YIR023W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DMA2(YNL116W)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains Gene:EMC1(YCL045C)|FD-Score:4.79|P-value:8.45E-7||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:EMC2(YJR088C)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:ERG4(YGL012W)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:GCV1(YDR019C)|FD-Score:4.5|P-value:3.37E-6||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GET2(YER083C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GET3(YDL100C)|FD-Score:7.57|P-value:1.88E-14||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GLO4(YOR040W)|FD-Score:3.29|P-value:5.10E-4||SGD DESC:Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:IMP2'(YIL154C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INP54(YOL065C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins Gene:JID1(YPR061C)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:KCS1(YDR017C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:LHP1(YDL051W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:RNA binding protein required for maturation of tRNA and U6 snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen Gene:LRO1(YNR008W)|FD-Score:8.3|P-value:5.18E-17||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:LSM1(YJL124C)|FD-Score:-3.89|P-value:4.95E-5||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LST4(YKL176C)|FD-Score:-4|P-value:3.19E-5||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAK10(YEL053C)|FD-Score:5.56|P-value:1.37E-8||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MIT1(YEL007W)|FD-Score:4.84|P-value:6.61E-7||SGD DESC:Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1 Gene:NHP10(YDL002C)|FD-Score:6.02|P-value:8.56E-10||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NUP42(YDR192C)|FD-Score:5.61|P-value:1.01E-8||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p Gene:NUR1(YDL089W)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OPI3(YJR073C)|FD-Score:4.92|P-value:4.40E-7||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OST3(YOR085W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins Gene:PCL6(YER059W)|FD-Score:4.29|P-value:8.99E-6||SGD DESC:Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding Gene:PDR1(YGL013C)|FD-Score:8.11|P-value:2.54E-16||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PFS1(YHR185C)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PHO88(YBR106W)|FD-Score:5.39|P-value:3.55E-8||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:REI1(YBR267W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RIM101(YHL027W)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RMD5(YDR255C)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain Gene:RPL27A(YHR010W)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPL34A(YER056C-A)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPS23A(YGR118W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RTT103(YDR289C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SBH2(YER019C-A)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p Gene:SIP1(YDR422C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions Gene:SLT2(YHR030C)|FD-Score:-4.71|P-value:1.26E-6||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF4(YGL115W)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SNF8(YPL002C)|FD-Score:-4.85|P-value:6.12E-7||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SRC1(YML034W)|FD-Score:9.06|P-value:6.49E-20||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:STP22(YCL008C)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SXM1(YDR395W)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:TAT1(YBR069C)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TOF1(YNL273W)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase Gene:TOM7(YNL070W)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:UBC8(YEL012W)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:UBP1(YDL122W)|FD-Score:7.95|P-value:9.27E-16||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UBP13(YBL067C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:UBP15(YMR304W)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p Gene:UFD2(YDL190C)|FD-Score:3.72|P-value:1.02E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:UPF3(YGR072W)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:VPS20(YMR077C)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS28(YPL065W)|FD-Score:-3.8|P-value:7.11E-5||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:-3.9|P-value:4.80E-5||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS61(YDR136C_d)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBR062C(YBR062C_p)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YBR197C(YBR197C_p)|FD-Score:7.97|P-value:7.78E-16||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YCH1(YGR203W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR056C(YDR056C_p)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YDR290W(YDR290W_d)|FD-Score:6.14|P-value:4.06E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YDR476C(YDR476C_p)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YER010C(YER010C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction Gene:YER084W(YER084W_p)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YET3(YDL072C)|FD-Score:5.66|P-value:7.36E-9||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YGR111W(YGR111W_p)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YHR180W(YHR180W_d)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML034C-A(YML034C-A_d)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR021W(YNR021W_p)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene Gene:YNR025C(YNR025C_d)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YNR040W(YNR040W_p)|FD-Score:8.69|P-value:1.88E-18||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNR048W(YNR048W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOL075C(YOL075C_p)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Putative ABC transporter Gene:YOR008C-A(YOR008C-A_p)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres Gene:YOR072W(YOR072W_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:AAH1(YNL141W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome Gene:APS1(YLR170C)|FD-Score:-4.2|P-value:1.34E-5||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ARG82(YDR173C)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARK1(YNL020C)|FD-Score:3.78|P-value:7.99E-5||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ARO80(YDR421W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids Gene:ATG3(YNR007C)|FD-Score:5.64|P-value:8.61E-9||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:ATP10(YLR393W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BCK1(YJL095W)|FD-Score:-6.35|P-value:1.07E-10||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BNI1(YNL271C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BST1(YFL025C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CDC73(YLR418C)|FD-Score:4.4|P-value:5.41E-6||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLB3(YDL155W)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:CLC1(YGR167W)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CNB1(YKL190W)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CPR8(YNR028W)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:CRT10(YOL063C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat Gene:CSF1(YLR087C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DAL81(YIR023W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DMA2(YNL116W)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains Gene:EMC1(YCL045C)|FD-Score:4.79|P-value:8.45E-7||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:EMC2(YJR088C)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:ERG4(YGL012W)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:GCV1(YDR019C)|FD-Score:4.5|P-value:3.37E-6||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GET2(YER083C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GET3(YDL100C)|FD-Score:7.57|P-value:1.88E-14||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GLO4(YOR040W)|FD-Score:3.29|P-value:5.10E-4||SGD DESC:Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:IMP2'(YIL154C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INP54(YOL065C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins Gene:JID1(YPR061C)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:KCS1(YDR017C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:LHP1(YDL051W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:RNA binding protein required for maturation of tRNA and U6 snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen Gene:LRO1(YNR008W)|FD-Score:8.3|P-value:5.18E-17||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:LSM1(YJL124C)|FD-Score:-3.89|P-value:4.95E-5||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LST4(YKL176C)|FD-Score:-4|P-value:3.19E-5||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAK10(YEL053C)|FD-Score:5.56|P-value:1.37E-8||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MIT1(YEL007W)|FD-Score:4.84|P-value:6.61E-7||SGD DESC:Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1 Gene:NHP10(YDL002C)|FD-Score:6.02|P-value:8.56E-10||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NUP42(YDR192C)|FD-Score:5.61|P-value:1.01E-8||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p Gene:NUR1(YDL089W)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OPI3(YJR073C)|FD-Score:4.92|P-value:4.40E-7||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OST3(YOR085W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins Gene:PCL6(YER059W)|FD-Score:4.29|P-value:8.99E-6||SGD DESC:Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding Gene:PDR1(YGL013C)|FD-Score:8.11|P-value:2.54E-16||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PFS1(YHR185C)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PHO88(YBR106W)|FD-Score:5.39|P-value:3.55E-8||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:REI1(YBR267W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RIM101(YHL027W)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RMD5(YDR255C)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain Gene:RPL27A(YHR010W)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPL34A(YER056C-A)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPS23A(YGR118W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RTT103(YDR289C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SBH2(YER019C-A)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p Gene:SIP1(YDR422C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions Gene:SLT2(YHR030C)|FD-Score:-4.71|P-value:1.26E-6||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF4(YGL115W)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SNF8(YPL002C)|FD-Score:-4.85|P-value:6.12E-7||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SRC1(YML034W)|FD-Score:9.06|P-value:6.49E-20||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:STP22(YCL008C)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SXM1(YDR395W)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:TAT1(YBR069C)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TOF1(YNL273W)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase Gene:TOM7(YNL070W)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:UBC8(YEL012W)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:UBP1(YDL122W)|FD-Score:7.95|P-value:9.27E-16||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UBP13(YBL067C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:UBP15(YMR304W)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p Gene:UFD2(YDL190C)|FD-Score:3.72|P-value:1.02E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:UPF3(YGR072W)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:VPS20(YMR077C)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS28(YPL065W)|FD-Score:-3.8|P-value:7.11E-5||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:-3.9|P-value:4.80E-5||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS61(YDR136C_d)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBR062C(YBR062C_p)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YBR197C(YBR197C_p)|FD-Score:7.97|P-value:7.78E-16||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YCH1(YGR203W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR056C(YDR056C_p)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YDR290W(YDR290W_d)|FD-Score:6.14|P-value:4.06E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YDR476C(YDR476C_p)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YER010C(YER010C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction Gene:YER084W(YER084W_p)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YET3(YDL072C)|FD-Score:5.66|P-value:7.36E-9||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YGR111W(YGR111W_p)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YHR180W(YHR180W_d)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML034C-A(YML034C-A_d)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR021W(YNR021W_p)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene Gene:YNR025C(YNR025C_d)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YNR040W(YNR040W_p)|FD-Score:8.69|P-value:1.88E-18||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNR048W(YNR048W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOL075C(YOL075C_p)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Putative ABC transporter Gene:YOR008C-A(YOR008C-A_p)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres Gene:YOR072W(YOR072W_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YMR290W-A_d8.652.69E-181.56YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YJR042W8.305.02E-171.56NUP85Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75
YBL105C6.747.95E-120.07PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YGR265W_d6.671.26E-110.82YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase
YMR203W5.852.47E-90.06TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YPR143W5.793.46E-90.02RRP15Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
YBR190W_d5.783.84E-90.28YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YMR268C5.492.01E-80.28PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YKL195W5.219.65E-80.05MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YOR116C5.161.24E-70.00RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YNR016C5.151.27E-70.11ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YER133W5.042.27E-70.33GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YJL143W4.721.20E-60.20TIM17Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
YDR464W4.523.09E-60.09SPP41Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3
YDR429C4.434.61E-60.13TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML034W9.066.49E-20SRC1Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance
YNR040W_p8.691.88E-18YNR040W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YNR008W8.305.18E-17LRO1Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase
YGL013C8.112.54E-16PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YBR197C_p7.977.78E-16YBR197C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication
YDL122W7.959.27E-16UBP1Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains
YDL100C7.571.88E-14GET3Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
YDR290W_d6.144.06E-10YDR290W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103
YDL002C6.028.56E-10NHP10Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair
YDL072C5.667.36E-9YET3Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
YNR007C5.648.61E-9ATG3E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p
YDR192C5.611.01E-8NUP42FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p
YEL053C5.561.37E-8MAK10Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible
YBR106W5.393.55E-8PHO88Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
YJR073C4.924.40E-7OPI3Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis

GO enrichment analysis for SGTC_188
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1482.20E-30SGTC_9221866-0035 480.0 μMChemDiv (Drug-like library)31561690.0526316excess fatty acid
0.1323.06E-24SGTC_7431275-0778 158.0 μMChemDiv (Drug-like library)28492570.0923077excess fatty acid
0.1323.32E-24SGTC_2508celastrol 40.5 μMMicrosource (Natural product library)1227240.0449438PDR1
0.1285.82E-23SGTC_15110906-3109 28.5 μMChemDiv (Drug-like library)28821800.0833333excess fatty acid
0.1173.39E-19SGTC_450kpi-0036 14.9 μMChemDiv (Drug-like library)27950210.0983607excess fatty acid
0.1125.01E-18SGTC_1150527-0192 112.0 μMChemDiv (Drug-like library)42339310.136364
0.1063.10E-16SGTC_352k048-0007 5.1 μMChemDiv (Drug-like library)68119110.241935ERAD & cell cycle
0.1056.62E-16SGTC_1953st076800 12.1 μMTimTec (Natural product derivative library)2975910.0806452
0.1057.17E-16SGTC_430570-0105 72.5 μMChemDiv (Drug-like library)11140460.0933333ERG2
0.1041.55E-15SGTC_1140364-0001 490.3 μMChemDiv (Drug-like library)40036550.0533333
0.1015.94E-15SGTC_1434032-1194 15.7 μMChemDiv (Drug-like library)18069630.114286
0.1001.17E-14SGTC_2659sanguinarine 24.2 μMMicrosource (Natural product library)133941490.0555556
0.0984.79E-14SGTC_1030844-0104 51.8 μMChemDiv (Drug-like library)53758040.129032ERAD & cell cycle
0.0977.01E-14SGTC_410α-linolenic acid 100.0 μMMiscellaneous8600.03125excess fatty acid
0.0961.43E-13SGTC_9841492-0255 2.9 μMChemDiv (Drug-like library)15851980.15873SWF1 & branched chain AA biosynthesis

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_243954920307.2 μM15722370Miscellaneous269.321643.83225
SGTC_3823229-096623.2 μM15722370ChemDiv (Drug-like library)269.321643.83225
SGTC_24435493264126.67 μM0.7441865340055Miscellaneous269.321642.39725
SGTC_2435570647610.6 μM0.6808515725375Miscellaneous297.37483.2925
SGTC_286k060-006722.9 μM0.6470595985051ChemDiv (Drug-like library)299.347623.81526tubulin folding & SWR complex
SGTC_623k072-010816.9 μM0.6444445382803ChemDiv (Drug-like library)254.31032.92315heme biosynthesis & mitochondrial translocase
SGTC_2424553792859.2 μM0.6382985382802Miscellaneous254.31032.92315
SGTC_2444548548436.41 μM0.6304356790631Miscellaneous269.321642.39725
SGTC_242556286817.85 μM0.56255714958Miscellaneous259.349964.02715
SGTC_24315490079200 μM0.56255722345Miscellaneous267.348824.5614