5615643

(5E)-3-[(3-chloro-4-methylanilino)methyl]-5-[(4-fluorophenyl)methylidene]-2-sulfanylidene-1,3-thiazolidin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1882
Screen concentration 20.0 μM
Source Miscellaneous
PubChem CID 2253075
SMILES CC1=C(C=C(C=C1)NCN2C(=O)C(=CC3=CC=C(C=C3)F)SC2=S)Cl
Standardized SMILES Cc1ccc(NCN2C(=S)SC(=Cc3ccc(F)cc3)C2=O)cc1Cl
Molecular weight 392.898
ALogP 5.84
H-bond donor count 1
H-bond acceptor count 5
Response signature TRP & mitochondrial translation

Pool Growth Kinetics
% growth inhibition (Het. pool) 22.48
% growth inhibition (Hom. pool) 11.7


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2253075
Download HIP data (tab-delimited text)  (excel)
Gene:ARC19(YKL013C)|FD-Score:3.25|P-value:5.81E-4|Clearance:0.07||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:BDP1(YNL039W)|FD-Score:-3.89|P-value:4.95E-5|Clearance:0||SGD DESC:Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs Gene:DED1(YOR204W)|FD-Score:3.35|P-value:3.98E-4|Clearance:0.01||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:ERG11(YHR007C)|FD-Score:4.76|P-value:9.80E-7|Clearance:0.53||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GPI18(YBR004C)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.04||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:IRR1(YIL026C)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.33||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:LIP1(YMR298W)|FD-Score:6.39|P-value:8.52E-11|Clearance:1.63||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:MMF1(YIL051C)|FD-Score:3.25|P-value:5.78E-4|Clearance:0||SGD DESC:Mitochondrial protein required for transamination of isoleucine but not of valine or leucine; may regulate specificity of branched-chain transaminases Bat1p and Bat2p; interacts genetically with mitochondrial ribosomal protein genes Gene:NOP15(YNL110C)|FD-Score:-5.2|P-value:9.98E-8|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP56(YLR197W)|FD-Score:-3.35|P-value:4.04E-4|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:RER2(YBR002C)|FD-Score:9.45|P-value:1.64E-21|Clearance:3.07||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPB8(YOR224C)|FD-Score:-3.26|P-value:5.60E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPP1(YHR062C)|FD-Score:4.23|P-value:1.19E-5|Clearance:0.27||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP14(YKL082C)|FD-Score:3.11|P-value:9.31E-4|Clearance:0.06||SGD DESC:Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family Gene:RSC8(YFR037C)|FD-Score:-3.15|P-value:8.23E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:UGP1(YKL035W)|FD-Score:3.96|P-value:3.79E-5|Clearance:0.27||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:YNL181W(YNL181W_p)|FD-Score:3.31|P-value:4.74E-4|Clearance:0.06||SGD DESC:Putative oxidoreductase; required for cell viability Gene:YPL251W(YPL251W_d)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C Gene:ARC19(YKL013C)|FD-Score:3.25|P-value:5.81E-4|Clearance:0.07||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:BDP1(YNL039W)|FD-Score:-3.89|P-value:4.95E-5|Clearance:0||SGD DESC:Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs Gene:DED1(YOR204W)|FD-Score:3.35|P-value:3.98E-4|Clearance:0.01||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:ERG11(YHR007C)|FD-Score:4.76|P-value:9.80E-7|Clearance:0.53||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GPI18(YBR004C)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.04||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:IRR1(YIL026C)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.33||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:LIP1(YMR298W)|FD-Score:6.39|P-value:8.52E-11|Clearance:1.63||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:MMF1(YIL051C)|FD-Score:3.25|P-value:5.78E-4|Clearance:0||SGD DESC:Mitochondrial protein required for transamination of isoleucine but not of valine or leucine; may regulate specificity of branched-chain transaminases Bat1p and Bat2p; interacts genetically with mitochondrial ribosomal protein genes Gene:NOP15(YNL110C)|FD-Score:-5.2|P-value:9.98E-8|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP56(YLR197W)|FD-Score:-3.35|P-value:4.04E-4|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:RER2(YBR002C)|FD-Score:9.45|P-value:1.64E-21|Clearance:3.07||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPB8(YOR224C)|FD-Score:-3.26|P-value:5.60E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPP1(YHR062C)|FD-Score:4.23|P-value:1.19E-5|Clearance:0.27||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP14(YKL082C)|FD-Score:3.11|P-value:9.31E-4|Clearance:0.06||SGD DESC:Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family Gene:RSC8(YFR037C)|FD-Score:-3.15|P-value:8.23E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:UGP1(YKL035W)|FD-Score:3.96|P-value:3.79E-5|Clearance:0.27||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:YNL181W(YNL181W_p)|FD-Score:3.31|P-value:4.74E-4|Clearance:0.06||SGD DESC:Putative oxidoreductase; required for cell viability Gene:YPL251W(YPL251W_d)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2253075
Download HOP data (tab-delimited text)  (excel)
Gene:ADH7(YCR105W)|FD-Score:-3.1|P-value:9.72E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance Gene:AEP2(YMR282C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM10(YER087W)|FD-Score:3.81|P-value:7.08E-5||SGD DESC:Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ARO1(YDR127W)|FD-Score:17.7|P-value:1.46E-70||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:4.43|P-value:4.74E-6||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARX1(YDR101C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex Gene:ATP3(YBR039W)|FD-Score:5.11|P-value:1.58E-7||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BLM10(YFL007W)|FD-Score:6.74|P-value:7.87E-12||SGD DESC:Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 Gene:BRP1(YGL007W_d)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BSC1(YDL037C)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:DPB4(YDR121W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization Gene:GCN2(YDR283C)|FD-Score:7.8|P-value:3.14E-15||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:4.92|P-value:4.29E-7||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:8.09|P-value:2.90E-16||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:18.4|P-value:5.00E-76||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GTF1(YGR102C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:GTR2(YGR163W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HMI1(YOL095C)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:IRA2(YOL081W)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:IRC19(YLL033W)|FD-Score:4.35|P-value:6.86E-6||SGD DESC:Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:5.75|P-value:4.41E-9||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LEM3(YNL323W)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LIP5(YOR196C)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LSB5(YCL034W)|FD-Score:-3.31|P-value:4.59E-4||SGD DESC:Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat Gene:MEF1(YLR069C)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MIP1(YOR330C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MRH4(YGL064C)|FD-Score:4.53|P-value:2.95E-6||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRP2(YPR166C)|FD-Score:7.44|P-value:5.13E-14||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL17(YNL252C)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MST1(YKL194C)|FD-Score:3.73|P-value:9.76E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:PAR32(YDL173W)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PET123(YOR158W)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PHO3(YBR092C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin Gene:PIF1(YML061C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PIM1(YBL022C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PPA2(YMR267W)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:RMD1(YDL001W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Cytoplasmic protein required for sporulation Gene:RPS29A(YLR388W)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RSM27(YGR215W)|FD-Score:6.88|P-value:3.08E-12||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SLM5(YCR024C)|FD-Score:7.09|P-value:6.77E-13||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SLX9(YGR081C)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SSD1(YDR293C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWT21(YNL187W)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:TRP1(YDR007W)|FD-Score:22.4|P-value:3.65E-111||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:10.2|P-value:1.51E-24||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:14.1|P-value:1.72E-45||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:10.7|P-value:4.06E-27||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:16.6|P-value:3.65E-62||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TRS85(YDR108W)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:TSC3(YBR058C-A)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:YDR008C(YDR008C_d)|FD-Score:12.8|P-value:8.40E-38||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Putative protein of unknown function Gene:YGL140C(YGL140C_p)|FD-Score:3.09|P-value:0.00100||SGD DESC:Putative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains Gene:YIL060W(YIL060W_p)|FD-Score:4|P-value:3.12E-5||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YJL043W(YJL043W_p)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Putative protein of unknown function; YJL043W is a non-essential gene Gene:YJL206C(YJL206C_p)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment Gene:YLR030W(YLR030W_p)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Putative protein of unknown function Gene:YNL184C(YNL184C_p)|FD-Score:4.3|P-value:8.60E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOL019W(YOL019W_p)|FD-Score:-3.12|P-value:9.15E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YOR072W(YOR072W_d)|FD-Score:-3.26|P-value:5.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL225W(YPL225W)|FD-Score:-5.09|P-value:1.80E-7||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:ADH7(YCR105W)|FD-Score:-3.1|P-value:9.72E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance Gene:AEP2(YMR282C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM10(YER087W)|FD-Score:3.81|P-value:7.08E-5||SGD DESC:Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ARO1(YDR127W)|FD-Score:17.7|P-value:1.46E-70||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:4.43|P-value:4.74E-6||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARX1(YDR101C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex Gene:ATP3(YBR039W)|FD-Score:5.11|P-value:1.58E-7||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BLM10(YFL007W)|FD-Score:6.74|P-value:7.87E-12||SGD DESC:Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 Gene:BRP1(YGL007W_d)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BSC1(YDL037C)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:DPB4(YDR121W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization Gene:GCN2(YDR283C)|FD-Score:7.8|P-value:3.14E-15||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:4.92|P-value:4.29E-7||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:8.09|P-value:2.90E-16||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:18.4|P-value:5.00E-76||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GTF1(YGR102C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:GTR2(YGR163W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HMI1(YOL095C)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:IRA2(YOL081W)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:IRC19(YLL033W)|FD-Score:4.35|P-value:6.86E-6||SGD DESC:Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:5.75|P-value:4.41E-9||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LEM3(YNL323W)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LIP5(YOR196C)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LSB5(YCL034W)|FD-Score:-3.31|P-value:4.59E-4||SGD DESC:Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat Gene:MEF1(YLR069C)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MIP1(YOR330C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MRH4(YGL064C)|FD-Score:4.53|P-value:2.95E-6||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRP2(YPR166C)|FD-Score:7.44|P-value:5.13E-14||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL17(YNL252C)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MST1(YKL194C)|FD-Score:3.73|P-value:9.76E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:PAR32(YDL173W)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PET123(YOR158W)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PHO3(YBR092C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin Gene:PIF1(YML061C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PIM1(YBL022C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PPA2(YMR267W)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:RMD1(YDL001W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Cytoplasmic protein required for sporulation Gene:RPS29A(YLR388W)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RSM27(YGR215W)|FD-Score:6.88|P-value:3.08E-12||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SLM5(YCR024C)|FD-Score:7.09|P-value:6.77E-13||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SLX9(YGR081C)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SSD1(YDR293C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWT21(YNL187W)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:TRP1(YDR007W)|FD-Score:22.4|P-value:3.65E-111||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:10.2|P-value:1.51E-24||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:14.1|P-value:1.72E-45||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:10.7|P-value:4.06E-27||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:16.6|P-value:3.65E-62||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TRS85(YDR108W)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:TSC3(YBR058C-A)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:YDR008C(YDR008C_d)|FD-Score:12.8|P-value:8.40E-38||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Putative protein of unknown function Gene:YGL140C(YGL140C_p)|FD-Score:3.09|P-value:0.00100||SGD DESC:Putative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains Gene:YIL060W(YIL060W_p)|FD-Score:4|P-value:3.12E-5||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YJL043W(YJL043W_p)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Putative protein of unknown function; YJL043W is a non-essential gene Gene:YJL206C(YJL206C_p)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment Gene:YLR030W(YLR030W_p)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Putative protein of unknown function Gene:YNL184C(YNL184C_p)|FD-Score:4.3|P-value:8.60E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOL019W(YOL019W_p)|FD-Score:-3.12|P-value:9.15E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YOR072W(YOR072W_d)|FD-Score:-3.26|P-value:5.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL225W(YPL225W)|FD-Score:-5.09|P-value:1.80E-7||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR002C9.451.64E-213.07RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YMR298W6.398.52E-111.63LIP1Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis
YHR007C4.769.80E-70.53ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YHR062C4.231.19E-50.27RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YKL035W3.963.79E-50.27UGP1UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
YIL026C3.691.13E-40.33IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YOR204W3.353.98E-40.01DED1ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility
YBR004C3.354.09E-40.04GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YNL181W_p3.314.74E-40.06YNL181W_pPutative oxidoreductase; required for cell viability
YIL051C3.255.78E-40.00MMF1Mitochondrial protein required for transamination of isoleucine but not of valine or leucine; may regulate specificity of branched-chain transaminases Bat1p and Bat2p; interacts genetically with mitochondrial ribosomal protein genes
YKL013C3.255.81E-40.07ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YPL251W_d3.187.42E-40.07YPL251W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
YKL082C3.119.31E-40.06RRP14Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family
YNL161W3.050.001150.11CBK1Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
YGR116W2.940.001650.03SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR007W22.403.65E-111TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YEL009C18.405.00E-76GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YDR127W17.701.46E-70ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YGL026C16.603.65E-62TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YKL211C14.101.72E-45TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR008C_d12.808.40E-38YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR354W10.704.06E-27TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YER090W10.201.51E-24TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YKR026C8.092.90E-16GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YDR283C7.803.14E-15GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YPR166C7.445.13E-14MRP2Mitochondrial ribosomal protein of the small subunit
YCR024C7.096.77E-13SLM5Mitochondrial asparaginyl-tRNA synthetase
YGR215W6.883.08E-12RSM27Mitochondrial ribosomal protein of the small subunit
YFL007W6.747.87E-12BLM10Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200
YKR019C5.754.41E-9IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1882
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.7520SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.238095TRP & mitochondrial translation
0.6410SGTC_18815652484 16.0 μMMiscellaneous22551290.375TRP & mitochondrial translation
0.6300SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.060241
0.5920SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.060241
0.5870SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.197183
0.5860SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.0921053
0.5720SGTC_24885268135 45.7 μMMiscellaneous12703560.171429
0.5660SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0288462
0.5600SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0752688
0.5500SGTC_1636st007707 68.9 μMTimTec (Natural product derivative library)4030510.0963855
0.5490SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.040404
0.5490SGTC_24545-benzylidenerhodanine 10.4 μMMiscellaneous12732100.238095TRP & mitochondrial translation
0.5420SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.047619
0.5400SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.113636
0.5360SGTC_12191469-0211 33.1 μMChemDiv (Drug-like library)68222820.116279

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_242856156437.78 μM12253075Miscellaneous392.8979635.84215
SGTC_8191013-025827.7 μM0.5901645753198ChemDiv (Drug-like library)390.90695.13415
SGTC_24375741531144.16 μM0.4571432262990Miscellaneous402.4392634.24617
SGTC_1921574013650 μM0.4117651774243Miscellaneous417.319485.30814TRP & mitochondrial translation
SGTC_2453573113222.18 μM0.406251362040Miscellaneous436.266794.16614
SGTC_1434032-119415.74 μM0.3906251806963ChemDiv (Drug-like library)329.3454833.69515
SGTC_2447574174233.78 μM0.3888891776504Miscellaneous433.318884.80515
SGTC_2450573973712.93 μM0.3846152262825Miscellaneous328.3607233.79315
SGTC_2041543-018462.41 μM0.3802822252213ChemDiv (Drug-like library)356.4138834.76515TRP & mitochondrial translation
SGTC_1881565248416 μM0.3752255129Miscellaneous417.319485.30814TRP & mitochondrial translation