st057356

1,2,12,13-tetramethoxy-7-methyl-3-nitro-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinoline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1886
Screen concentration 49.9 μM
Source TimTec (Natural product derivative library)
PubChem CID 24207747
SMILES CN1CCC2=C(C(=C(C3=C2C1CC4=CC(=C(C=C43)OC)OC)OC)OC)[N+](=O)[O-]
Standardized SMILES COc1cc2CC3N(C)CCc4c3c(c(OC)c(OC)c4[N+](=O)[O-])c2cc1OC
Molecular weight 400.4251
ALogP 3.43
H-bond donor count 0
H-bond acceptor count 7
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 19.88
% growth inhibition (Hom. pool) 4.65


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 24207747
Download HIP data (tab-delimited text)  (excel)
Gene:EXO84(YBR102C)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.03||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:GCD11(YER025W)|FD-Score:-3.9|P-value:4.72E-5|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GPI13(YLL031C)|FD-Score:3.52|P-value:2.14E-4|Clearance:0.02||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:HEM12(YDR047W)|FD-Score:3.84|P-value:6.14E-5|Clearance:0.01||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HEM3(YDL205C)|FD-Score:3.58|P-value:1.72E-4|Clearance:0.06||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:KOG1(YHR186C)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:LSG1(YGL099W)|FD-Score:6.15|P-value:3.77E-10|Clearance:2.31||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:9.03|P-value:8.51E-20|Clearance:2.88||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NSA2(YER126C)|FD-Score:3.63|P-value:1.40E-4|Clearance:0.03||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:POL1(YNL102W)|FD-Score:3.23|P-value:6.09E-4|Clearance:0.11||SGD DESC:Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis Gene:PRI2(YKL045W)|FD-Score:3.31|P-value:4.59E-4|Clearance:0.06||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PRP16(YKR086W)|FD-Score:3.83|P-value:6.53E-5|Clearance:0.16||SGD DESC:DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step Gene:RPL25(YOL127W)|FD-Score:-3.99|P-value:3.37E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:SRP1(YNL189W)|FD-Score:3.12|P-value:8.99E-4|Clearance:0.03||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TID3(YIL144W)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.06||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:TOM40(YMR203W)|FD-Score:3.26|P-value:5.66E-4|Clearance:0.02||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRS31(YDR472W)|FD-Score:3.44|P-value:2.96E-4|Clearance:0.12||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UBC9(YDL064W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.03||SGD DESC:SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) Gene:YGR190C(YGR190C_d)|FD-Score:3.5|P-value:2.29E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Gene:EXO84(YBR102C)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.03||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:GCD11(YER025W)|FD-Score:-3.9|P-value:4.72E-5|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GPI13(YLL031C)|FD-Score:3.52|P-value:2.14E-4|Clearance:0.02||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:HEM12(YDR047W)|FD-Score:3.84|P-value:6.14E-5|Clearance:0.01||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HEM3(YDL205C)|FD-Score:3.58|P-value:1.72E-4|Clearance:0.06||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:KOG1(YHR186C)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:LSG1(YGL099W)|FD-Score:6.15|P-value:3.77E-10|Clearance:2.31||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:9.03|P-value:8.51E-20|Clearance:2.88||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NSA2(YER126C)|FD-Score:3.63|P-value:1.40E-4|Clearance:0.03||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:POL1(YNL102W)|FD-Score:3.23|P-value:6.09E-4|Clearance:0.11||SGD DESC:Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis Gene:PRI2(YKL045W)|FD-Score:3.31|P-value:4.59E-4|Clearance:0.06||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PRP16(YKR086W)|FD-Score:3.83|P-value:6.53E-5|Clearance:0.16||SGD DESC:DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step Gene:RPL25(YOL127W)|FD-Score:-3.99|P-value:3.37E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:SRP1(YNL189W)|FD-Score:3.12|P-value:8.99E-4|Clearance:0.03||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TID3(YIL144W)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.06||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:TOM40(YMR203W)|FD-Score:3.26|P-value:5.66E-4|Clearance:0.02||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRS31(YDR472W)|FD-Score:3.44|P-value:2.96E-4|Clearance:0.12||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UBC9(YDL064W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.03||SGD DESC:SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) Gene:YGR190C(YGR190C_d)|FD-Score:3.5|P-value:2.29E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 24207747
Download HOP data (tab-delimited text)  (excel)
Gene:ACN9(YDR511W)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes Gene:AFT1(YGL071W)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ART5(YGR068C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane Gene:AVT1(YJR001W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BAG7(YOR134W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:CCC2(YDR270W)|FD-Score:3.9|P-value:4.87E-5||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CLN2(YPL256C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:COX10(YPL172C)|FD-Score:4.49|P-value:3.57E-6||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CSI1(YMR025W)|FD-Score:3.76|P-value:8.36E-5||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome Gene:CTF3(YLR381W)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6 Gene:DGK1(YOR311C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Diacylglycerol kinase, localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain Gene:DLD2(YDL178W)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:EDS1(YBR033W_p)|FD-Score:-3.89|P-value:5.05E-5||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:FLC2(YAL053W)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:GEA2(YEL022W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GIS4(YML006C)|FD-Score:6.35|P-value:1.09E-10||SGD DESC:CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway Gene:HCM1(YCR065W)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role Gene:HOS1(YPR068C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:HST1(YOL068C)|FD-Score:7.96|P-value:8.72E-16||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HTB2(YBL002W)|FD-Score:-3.8|P-value:7.22E-5||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:LCL2(YLR104W_p)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:MAM1(YER106W)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MGA2(YIR033W)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MRP2(YPR166C)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL40(YPL173W)|FD-Score:-3.13|P-value:8.60E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NIP100(YPL174C)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:OMA1(YKR087C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:PCL8(YPL219W)|FD-Score:4.63|P-value:1.80E-6||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PDR1(YGL013C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEP8(YJL053W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PMT3(YOR321W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:PTR2(YKR093W)|FD-Score:4.3|P-value:8.66E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:REV7(YIL139C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RIM101(YHL027W)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:ROY1(YMR258C)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPA49(YNL248C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:RNA polymerase I subunit A49 Gene:RPS10B(YMR230W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:SAK1(YER129W)|FD-Score:6.42|P-value:6.87E-11||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SAL1(YNL083W)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:ADP/ATP transporter; member of the Ca2+-binding subfamily of mitochondrial carriers, with two EF-hand motifs; transport activity of either Sal1p or Pet9p is critical for viability; polymorphic in different S. cerevisiae strains Gene:SNU66(YOR308C)|FD-Score:-3.84|P-value:6.27E-5||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:SRS2(YJL092W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SYN8(YAL014C)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:TAD1(YGL243W)|FD-Score:4.42|P-value:4.86E-6||SGD DESC:tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala Gene:THI2(YBR240C)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:TRK1(YJL129C)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:UPS2(YLR168C)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:VPS5(YOR069W)|FD-Score:4.87|P-value:5.45E-7||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:YBR206W(YBR206W_d)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YCR023C(YCR023C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YFL052W(YFL052W_p)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity Gene:YHR139C-A(YHR139C-A_d)|FD-Score:8.76|P-value:1.01E-18||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL091C(YKL091C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YKL107W(YKL107W_p)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YML122C(YML122C_d)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR230W-A(YMR230W-A_p)|FD-Score:-3.75|P-value:9.02E-5||SGD DESC:Putative protein of unknown function Gene:YOR022C(YOR022C_p)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR318C(YOR318C_d)|FD-Score:4.39|P-value:5.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YOR343C(YOR343C_d)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ACN9(YDR511W)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes Gene:AFT1(YGL071W)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ART5(YGR068C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane Gene:AVT1(YJR001W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BAG7(YOR134W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:CCC2(YDR270W)|FD-Score:3.9|P-value:4.87E-5||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CLN2(YPL256C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:COX10(YPL172C)|FD-Score:4.49|P-value:3.57E-6||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CSI1(YMR025W)|FD-Score:3.76|P-value:8.36E-5||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome Gene:CTF3(YLR381W)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6 Gene:DGK1(YOR311C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Diacylglycerol kinase, localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain Gene:DLD2(YDL178W)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:EDS1(YBR033W_p)|FD-Score:-3.89|P-value:5.05E-5||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:FLC2(YAL053W)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:GEA2(YEL022W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GIS4(YML006C)|FD-Score:6.35|P-value:1.09E-10||SGD DESC:CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway Gene:HCM1(YCR065W)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role Gene:HOS1(YPR068C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:HST1(YOL068C)|FD-Score:7.96|P-value:8.72E-16||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HTB2(YBL002W)|FD-Score:-3.8|P-value:7.22E-5||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:LCL2(YLR104W_p)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:MAM1(YER106W)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MGA2(YIR033W)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MRP2(YPR166C)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL40(YPL173W)|FD-Score:-3.13|P-value:8.60E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NIP100(YPL174C)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:OMA1(YKR087C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:PCL8(YPL219W)|FD-Score:4.63|P-value:1.80E-6||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PDR1(YGL013C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEP8(YJL053W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PMT3(YOR321W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:PTR2(YKR093W)|FD-Score:4.3|P-value:8.66E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:REV7(YIL139C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RIM101(YHL027W)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:ROY1(YMR258C)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPA49(YNL248C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:RNA polymerase I subunit A49 Gene:RPS10B(YMR230W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:SAK1(YER129W)|FD-Score:6.42|P-value:6.87E-11||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SAL1(YNL083W)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:ADP/ATP transporter; member of the Ca2+-binding subfamily of mitochondrial carriers, with two EF-hand motifs; transport activity of either Sal1p or Pet9p is critical for viability; polymorphic in different S. cerevisiae strains Gene:SNU66(YOR308C)|FD-Score:-3.84|P-value:6.27E-5||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:SRS2(YJL092W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SYN8(YAL014C)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:TAD1(YGL243W)|FD-Score:4.42|P-value:4.86E-6||SGD DESC:tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala Gene:THI2(YBR240C)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:TRK1(YJL129C)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:UPS2(YLR168C)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:VPS5(YOR069W)|FD-Score:4.87|P-value:5.45E-7||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:YBR206W(YBR206W_d)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YCR023C(YCR023C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YFL052W(YFL052W_p)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity Gene:YHR139C-A(YHR139C-A_d)|FD-Score:8.76|P-value:1.01E-18||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL091C(YKL091C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YKL107W(YKL107W_p)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YML122C(YML122C_d)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR230W-A(YMR230W-A_p)|FD-Score:-3.75|P-value:9.02E-5||SGD DESC:Putative protein of unknown function Gene:YOR022C(YOR022C_p)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR318C(YOR318C_d)|FD-Score:4.39|P-value:5.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YOR343C(YOR343C_d)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR170W9.038.51E-202.88NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YGL099W6.153.77E-102.31LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YDR047W3.846.14E-50.01HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YKR086W3.836.53E-50.16PRP16DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step
YBR102C3.671.23E-40.03EXO84Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
YER126C3.631.40E-40.03NSA2Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA
YDL064W3.611.55E-40.03UBC9SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC)
YDL205C3.581.72E-40.06HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YLL031C3.522.14E-40.02GPI13ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein
YGR190C_d3.502.29E-40.00YGR190C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
YIL144W3.502.33E-40.06TID3Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
YDR472W3.442.96E-40.12TRS31One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YKL045W3.314.59E-40.06PRI2Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YMR203W3.265.66E-40.02TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YNL102W3.236.09E-40.11POL1Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR139C-A_d8.761.01E-18YHR139C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOL068C7.968.72E-16HST1NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance
YER129W6.426.87E-11SAK1Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome
YML006C6.351.09E-10GIS4CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway
YOR069W4.875.45E-7VPS5Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes
YPL219W4.631.80E-6PCL8Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation
YPL172C4.493.57E-6COX10Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders
YGL243W4.424.86E-6TAD1tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala
YOR318C_d4.395.55E-6YOR318C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo
YKR093W4.308.66E-6PTR2Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p
YIR033W4.211.25E-5MGA2ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting
YKL091C4.211.26E-5YKL091CPutative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study
YOR343C_d4.211.29E-5YOR343C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YJL053W4.201.32E-5PEP8Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
YKL107W_p4.181.43E-5YKL107W_pPutative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein

GO enrichment analysis for SGTC_1886
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1731.03E-40SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.034482860S ribosome export
0.1646.49E-37SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.13095260S ribosome export
0.1632.35E-36SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.091836760S ribosome export
0.1533.85E-32SGTC_32769139060 49.5 μMChembridge (Drug-like library)48706190.041666760S ribosome export
0.1461.06E-29SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.096385560S ribosome export
0.1461.82E-29SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.035714360S ribosome export
0.1447.32E-29SGTC_22047233102 200.0 μMChembridge (Fragment library)21724800.036144660S ribosome export
0.1412.08E-27SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.036585460S ribosome export
0.1402.69E-27SGTC_623k072-0108 16.9 μMChemDiv (Drug-like library)53828030.0243902heme biosynthesis & mitochondrial translocase
0.1397.01E-27SGTC_31709103054 49.5 μMChembridge (Drug-like library)164329700.045454560S ribosome export
0.1373.44E-26SGTC_1350933-0004 26.3 μMChemDiv (Drug-like library)212306170.023255860S ribosome export
0.1352.00E-25SGTC_490717-0965 32.1 μMChemDiv (Drug-like library)58893490.12345760S ribosome export
0.1338.20E-25SGTC_850868-0259 160.1 μMChemDiv (Drug-like library)831730.10958960S ribosome export
0.1283.82E-23SGTC_7064262-0057 46.9 μMChemDiv (Drug-like library)8832980.1fatty acid desaturase (OLE1)
0.1285.27E-23SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.078947460S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1855st05619539.6 μM0.6909094028379TimTec (Natural product derivative library)434.323564.2820560S ribosome export
SGTC_1126isoteolin1.02 μM0.483871133323TimTec (Natural product library)327.374343.08325
SGTC_445e6 berbamine66 μM0.3440866610268ICCB bioactive library757.826928.56011NEO1-PIK1
SGTC_2569tetrandrine100 μM0.32573078ICCB bioactive library622.749887.21808
SGTC_491tetrandrine80.3 μM0.32573078ICCB bioactive library622.749887.21808
SGTC_1718roemerine11.93 μM0.295775235224TimTec (Natural product derivative library)279.333083.36803redox potentiating
SGTC_1083didemethylchlorpromazine sulfoxide707.39 nM0.279412398788TimTec (Natural product library)353.411623.81805redox potentiating
SGTC_1763st04448294.5 μM0.26666788505TimTec (Natural product derivative library)211.171461.45105copper-dependent oxidative stress
SGTC_460trequinsin123 μM0.259745537Miscellaneous405.489484.48604
SGTC_1531st05929752.4 μM0.256417566702TimTec (Pure natural product library)457.517685.22206
SGTC_16erodoxin22.81 μM0.253967339ChemDiv (Drug-like library)277.0292.3505unfolded protein response