3970-0742

1-(4-bromo-2-nitrophenyl)pyrrole-2,5-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_189
Screen concentration 2.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 2891807
SMILES C1=CC(=C(C=C1Br)[N+](=O)[O-])N2C(=O)C=CC2=O
Standardized SMILES [O-][N+](=O)c1cc(Br)ccc1N2C(=O)C=CC2=O
Molecular weight 297.0617
ALogP 2.03
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 27.95
% growth inhibition (Hom. pool) 9.77


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2891807
Download HIP data (tab-delimited text)  (excel)
Gene:AVO1(YOL078W)|FD-Score:3.19|P-value:7.12E-4|Clearance:0.04||SGD DESC:Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:CDC23(YHR166C)|FD-Score:8.37|P-value:2.87E-17|Clearance:4.5||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:FRS1(YLR060W)|FD-Score:-3.38|P-value:3.65E-4|Clearance:0||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:HSP10(YOR020C)|FD-Score:3.24|P-value:5.93E-4|Clearance:0.05||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:KRI1(YNL308C)|FD-Score:-3.26|P-value:5.51E-4|Clearance:0||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:PRE2(YPR103W)|FD-Score:3.87|P-value:5.54E-5|Clearance:0.05||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RPR2(YIR015W)|FD-Score:-4.08|P-value:2.28E-5|Clearance:0||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:SLU7(YDR088C)|FD-Score:3.15|P-value:8.17E-4|Clearance:0.12||SGD DESC:RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain Gene:UTP13(YLR222C)|FD-Score:3.81|P-value:6.83E-5|Clearance:0.57||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YJL086C(YJL086C_d)|FD-Score:-3.59|P-value:1.68E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:AVO1(YOL078W)|FD-Score:3.19|P-value:7.12E-4|Clearance:0.04||SGD DESC:Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:CDC23(YHR166C)|FD-Score:8.37|P-value:2.87E-17|Clearance:4.5||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:FRS1(YLR060W)|FD-Score:-3.38|P-value:3.65E-4|Clearance:0||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:HSP10(YOR020C)|FD-Score:3.24|P-value:5.93E-4|Clearance:0.05||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:KRI1(YNL308C)|FD-Score:-3.26|P-value:5.51E-4|Clearance:0||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:PRE2(YPR103W)|FD-Score:3.87|P-value:5.54E-5|Clearance:0.05||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RPR2(YIR015W)|FD-Score:-4.08|P-value:2.28E-5|Clearance:0||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:SLU7(YDR088C)|FD-Score:3.15|P-value:8.17E-4|Clearance:0.12||SGD DESC:RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain Gene:UTP13(YLR222C)|FD-Score:3.81|P-value:6.83E-5|Clearance:0.57||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YJL086C(YJL086C_d)|FD-Score:-3.59|P-value:1.68E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2891807
Download HOP data (tab-delimited text)  (excel)
Gene:AAC3(YBR085W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:AIM24(YJR080C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss Gene:AMN1(YBR158W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:APE4(YHR113W)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family Gene:CYS3(YAL012W)|FD-Score:4.83|P-value:6.76E-7||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DGR2(YKL121W_p)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DMC1(YER179W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:FMP10(YER182W_p)|FD-Score:7.01|P-value:1.15E-12||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HEF3(YNL014W)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HUL4(YJR036C)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:ILV6(YCL009C)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:KIP1(YBL063W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions Gene:MLS1(YNL117W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:PET122(YER153C)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PGC1(YPL206C)|FD-Score:-3.78|P-value:7.95E-5||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PLB3(YOL011W)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:POA1(YBR022W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:PTK1(YKL198C)|FD-Score:4.56|P-value:2.62E-6||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:RAS2(YNL098C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RPL26A(YLR344W)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:SIS2(YKR072C)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SWF1(YDR126W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TRP2(YER090W)|FD-Score:-4.15|P-value:1.69E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP4(YDR354W)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP13(YBL067C)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:URA1(YKL216W)|FD-Score:4.51|P-value:3.19E-6||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VHR2(YER064C_p)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBL059W(YBL059W_p)|FD-Score:4.49|P-value:3.50E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YDR008C(YDR008C_d)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL077C(YIL077C_p)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YIR042C(YIR042C_p)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YML079W(YML079W_p)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm Gene:YNL211C(YNL211C_p)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YOL131W(YOL131W_p)|FD-Score:4.82|P-value:7.05E-7||SGD DESC:Putative protein of unknown function Gene:YOR186W(YOR186W_p)|FD-Score:-4.33|P-value:7.43E-6||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YPL102C(YPL102C_d)|FD-Score:-4|P-value:3.11E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:AAC3(YBR085W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:AIM24(YJR080C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss Gene:AMN1(YBR158W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:APE4(YHR113W)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family Gene:CYS3(YAL012W)|FD-Score:4.83|P-value:6.76E-7||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DGR2(YKL121W_p)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DMC1(YER179W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:FMP10(YER182W_p)|FD-Score:7.01|P-value:1.15E-12||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HEF3(YNL014W)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HUL4(YJR036C)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:ILV6(YCL009C)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:KIP1(YBL063W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions Gene:MLS1(YNL117W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:PET122(YER153C)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PGC1(YPL206C)|FD-Score:-3.78|P-value:7.95E-5||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PLB3(YOL011W)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:POA1(YBR022W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:PTK1(YKL198C)|FD-Score:4.56|P-value:2.62E-6||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:RAS2(YNL098C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RPL26A(YLR344W)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:SIS2(YKR072C)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SWF1(YDR126W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TRP2(YER090W)|FD-Score:-4.15|P-value:1.69E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP4(YDR354W)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP13(YBL067C)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:URA1(YKL216W)|FD-Score:4.51|P-value:3.19E-6||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VHR2(YER064C_p)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBL059W(YBL059W_p)|FD-Score:4.49|P-value:3.50E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YDR008C(YDR008C_d)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL077C(YIL077C_p)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YIR042C(YIR042C_p)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YML079W(YML079W_p)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm Gene:YNL211C(YNL211C_p)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YOL131W(YOL131W_p)|FD-Score:4.82|P-value:7.05E-7||SGD DESC:Putative protein of unknown function Gene:YOR186W(YOR186W_p)|FD-Score:-4.33|P-value:7.43E-6||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YPL102C(YPL102C_d)|FD-Score:-4|P-value:3.11E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR166C8.372.87E-174.50CDC23Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YPR103W3.875.54E-50.05PRE2Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
YLR222C3.816.83E-50.57UTP13Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YOR020C3.245.93E-40.05HSP10Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES
YOL078W3.197.12E-40.04AVO1Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth
YDR088C3.158.17E-40.12SLU7RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
YLR197W3.030.001220.09NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YDR413C_d2.940.001640.07YDR413C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing
YGL008C2.870.002080.10PMA1Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]
YNL062C2.760.002880.13GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YGR280C2.630.004290.01PXR1Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain
YOL146W2.610.004460.10PSF3Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YJL111W2.510.005970.01CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YPR137W2.500.006150.05RRP9Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
YDR427W2.450.007130.01RPN9Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER182W_p7.011.15E-12FMP10_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YAL012W4.836.76E-7CYS3Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress
YOL131W_p4.827.05E-7YOL131W_pPutative protein of unknown function
YKL198C4.562.62E-6PTK1Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein
YKL216W4.513.19E-6URA1Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid
YBL059W_p4.493.50E-6YBL059W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication
YDR126W4.141.77E-5SWF1Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
YLR344W3.944.04E-5RPL26ARibosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication
YBR085W3.711.04E-4AAC3Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication
YIL077C_p3.591.67E-4YIL077C_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
YNL098C3.512.28E-4RAS2GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes
YBR022W3.403.32E-4POA1Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing
YBL063W3.403.38E-4KIP1Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions
YER179W3.373.79E-4DMC1Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p
YKL121W_p3.344.12E-4DGR2_pProtein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds

GO enrichment analysis for SGTC_189
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1411.47E-27SGTC_1903970-0790 3.7 μMChemDiv (Drug-like library)28323520.24
0.0872.35E-11SGTC_9841492-0255 2.9 μMChemDiv (Drug-like library)15851980.0491803SWF1 & branched chain AA biosynthesis
0.0808.55E-10SGTC_1863455-3465 67.3 μMChemDiv (Drug-like library)7552780.0714286
0.0791.09E-9SGTC_1503970-0969 77.2 μMChemDiv (Drug-like library)66156400.189189
0.0791.44E-9SGTC_2144373-3496 70.0 μMChemDiv (Drug-like library)37791660.1
0.0757.60E-9SGTC_1070358-0022 2.7 μMChemDiv (Drug-like library)5211060.0612245SWF1 & branched chain AA biosynthesis
0.0757.88E-9SGTC_14244048-1941 275.0 μMChemDiv (Drug-like library)X14240.103448
0.0741.16E-8SGTC_340469-0683 81.8 μMChemDiv (Drug-like library)36909230.222222SWF1 & branched chain AA biosynthesis
0.0714.52E-8SGTC_13481493-0373 93.8 μMChemDiv (Drug-like library)30101290.0555556
0.0672.46E-7SGTC_1696st025591 34.8 μMTimTec (Natural product derivative library)27871470.186441SWF1 & branched chain AA biosynthesis
0.0664.12E-7SGTC_1659st012954 42.1 μMTimTec (Natural product derivative library)242072620.0512821
0.0655.61E-7SGTC_1859st055998 20.0 μMTimTec (Natural product derivative library)6763010.0819672
0.0656.42E-7SGTC_31609100216 49.5 μMChembridge (Drug-like library)171910390.0821918
0.0649.66E-7SGTC_8420271-0008 11.1 μMChemDiv (Drug-like library)12217090.0961538heme biosynthesis & mitochondrial translocase
0.0631.33E-6SGTC_28839059145 45.5 μMChembridge (Drug-like library)164765100.0769231

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8470342-02745.54 μM0.618097ChemDiv (Drug-like library)218.165641.28204
SGTC_196k015-002318.11 μM0.385211998ChemDiv (Drug-like library)361.146963.10204PDR1
SGTC_1340269-00186.92 μM0.3754378915ChemDiv (Drug-like library)411.121283.04617
SGTC_7930488-0545106 μM0.372093292548ChemDiv (Drug-like library)237.212042.24105
SGTC_1027k015-00258.67 μM0.3584913598955ChemDiv (Drug-like library)361.146963.10204
SGTC_1683970-0862530.54 μM0.3064523135186ChemDiv (Drug-like library)330.292160.54116
SGTC_3763970-086259.1 μM0.3064523135186ChemDiv (Drug-like library)330.292160.54116
SGTC_16erodoxin22.81 μM0.3023263967339ChemDiv (Drug-like library)277.0292.3505unfolded protein response
SGTC_1471k673-031237 μM0.29824624099884ChemDiv (Drug-like library)439.28043.4505RNA processing & uracil transport
SGTC_10483161-030024.5 μM0.2916672165101ChemDiv (Drug-like library)266.090722.24102
SGTC_600986-0278202 μM0.2777783110598ChemDiv (Drug-like library)415.056744.56703