st059073

3-bromo-6-fluorochromen-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1891
Screen concentration 26.2 μM
Source TimTec (Natural product derivative library)
PubChem CID 688754
SMILES C1=CC2=C(C=C1F)C(=O)C(=CO2)Br
Standardized SMILES Fc1ccc2OC=C(Br)C(=O)c2c1
Molecular weight 243.0293
ALogP 2.05
H-bond donor count 0
H-bond acceptor count 3
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.6
% growth inhibition (Hom. pool) 4.65


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 688754
Download HIP data (tab-delimited text)  (excel)
Gene:CDC8(YJR057W)|FD-Score:3.54|P-value:2.03E-4|Clearance:0.02||SGD DESC:Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p Gene:CDC9(YDL164C)|FD-Score:3.3|P-value:4.85E-4|Clearance:0.27||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:ERB1(YMR049C)|FD-Score:3.31|P-value:4.63E-4|Clearance:0.01||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:GCD1(YOR260W)|FD-Score:4.04|P-value:2.65E-5|Clearance:0.21||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD2(YGR083C)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.09||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI13(YLL031C)|FD-Score:4.3|P-value:8.46E-6|Clearance:0.17||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:MIA40(YKL195W)|FD-Score:3.43|P-value:2.96E-4|Clearance:0.12||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NAR1(YNL240C)|FD-Score:4.94|P-value:3.97E-7|Clearance:0.63||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:NIC96(YFR002W)|FD-Score:-4.23|P-value:1.16E-5|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NOP56(YLR197W)|FD-Score:-4.78|P-value:8.65E-7|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:STS1(YIR011C)|FD-Score:3.81|P-value:7.06E-5|Clearance:0.27||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:YDR413C(YDR413C_d)|FD-Score:3.52|P-value:2.16E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YOR146W(YOR146W_d)|FD-Score:3.83|P-value:6.32E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YOR282W(YOR282W_d)|FD-Score:5.33|P-value:4.89E-8|Clearance:0.63||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C Gene:CDC8(YJR057W)|FD-Score:3.54|P-value:2.03E-4|Clearance:0.02||SGD DESC:Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p Gene:CDC9(YDL164C)|FD-Score:3.3|P-value:4.85E-4|Clearance:0.27||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:ERB1(YMR049C)|FD-Score:3.31|P-value:4.63E-4|Clearance:0.01||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:GCD1(YOR260W)|FD-Score:4.04|P-value:2.65E-5|Clearance:0.21||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD2(YGR083C)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.09||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPI13(YLL031C)|FD-Score:4.3|P-value:8.46E-6|Clearance:0.17||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:MIA40(YKL195W)|FD-Score:3.43|P-value:2.96E-4|Clearance:0.12||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NAR1(YNL240C)|FD-Score:4.94|P-value:3.97E-7|Clearance:0.63||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:NIC96(YFR002W)|FD-Score:-4.23|P-value:1.16E-5|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NOP56(YLR197W)|FD-Score:-4.78|P-value:8.65E-7|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:STS1(YIR011C)|FD-Score:3.81|P-value:7.06E-5|Clearance:0.27||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:YDR413C(YDR413C_d)|FD-Score:3.52|P-value:2.16E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YOR146W(YOR146W_d)|FD-Score:3.83|P-value:6.32E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YOR282W(YOR282W_d)|FD-Score:5.33|P-value:4.89E-8|Clearance:0.63||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 688754
Download HOP data (tab-delimited text)  (excel)
Gene:ARK1(YNL020C)|FD-Score:-3.19|P-value:7.01E-4||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ARR3(YPR201W)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:ATP4(YPL078C)|FD-Score:6.42|P-value:6.95E-11||SGD DESC:Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated Gene:BCH2(YKR027W)|FD-Score:4.52|P-value:3.07E-6||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BUD16(YEL029C)|FD-Score:7.24|P-value:2.24E-13||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:BUD25(YER014C-A)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:CCP1(YKR066C)|FD-Score:4.5|P-value:3.32E-6||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CCS1(YMR038C)|FD-Score:4.5|P-value:3.42E-6||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CPR8(YNR028W)|FD-Score:3.75|P-value:8.93E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:CTI6(YPL181W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:DAL7(YIR031C)|FD-Score:4.01|P-value:3.00E-5||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:ECM4(YKR076W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ELP6(YMR312W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERG3(YLR056W)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERI1(YPL096C-A)|FD-Score:5.62|P-value:9.48E-9||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:EXG1(YLR300W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:FAP1(YNL023C)|FD-Score:4.49|P-value:3.56E-6||SGD DESC:Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 Gene:HEM14(YER014W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HFI1(YPL254W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HXT17(YNR072W)|FD-Score:4.53|P-value:2.91E-6||SGD DESC:Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose Gene:ICP55(YER078C)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family Gene:IRC19(YLL033W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci Gene:MAC1(YMR021C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDM38(YOL027C)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MOG1(YJR074W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MRPL10(YNL284C)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:MRPS8(YMR158W)|FD-Score:4.87|P-value:5.47E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSH1(YHR120W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MTG2(YHR168W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly Gene:MUS81(YDR386W)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:OAZ1(YPL052W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:PDH1(YPR002W)|FD-Score:5.27|P-value:6.96E-8||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PET100(YDR079W)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PRE9(YGR135W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:PRS3(YHL011C)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RAD52(YML032C)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:RPL22B(YFL034C-A)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPS1B(YML063W)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication Gene:RTC6(YPL183W-A)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SAL1(YNL083W)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:ADP/ATP transporter; member of the Ca2+-binding subfamily of mitochondrial carriers, with two EF-hand motifs; transport activity of either Sal1p or Pet9p is critical for viability; polymorphic in different S. cerevisiae strains Gene:SNA3(YJL151C)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles Gene:SNF2(YOR290C)|FD-Score:-3.34|P-value:4.18E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SOD1(YJR104C)|FD-Score:6.21|P-value:2.68E-10||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SRL2(YLR082C)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation Gene:STD1(YOR047C)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:TOS1(YBR162C)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TSC3(YBR058C-A)|FD-Score:23.5|P-value:4.20E-122||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:4.29|P-value:8.95E-6||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VAC17(YCL063W)|FD-Score:5.96|P-value:1.23E-9||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VPS3(YDR495C)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS45(YGL095C)|FD-Score:7.02|P-value:1.12E-12||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBR113W(YBR113W_d)|FD-Score:4.29|P-value:8.98E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YBR174C(YBR174C_d)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR300C(YBR300C_d)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YCL074W(YCL074W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDR271C(YDR271C_d)|FD-Score:-3.57|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YEA6(YEL006W)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YGL185C(YGL185C_p)|FD-Score:3.3|P-value:4.92E-4||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR051C(YGR051C_d)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Gene:YGR111W(YGR111W_p)|FD-Score:-3.18|P-value:7.28E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YIL067C(YIL067C_p)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YJL163C(YJL163C_p)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Putative protein of unknown function Gene:YMC1(YPR058W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC1 has a paralog, YMC2, that arose from the whole genome duplication Gene:ZAP1(YJL056C)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ARK1(YNL020C)|FD-Score:-3.19|P-value:7.01E-4||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ARR3(YPR201W)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:ATP4(YPL078C)|FD-Score:6.42|P-value:6.95E-11||SGD DESC:Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated Gene:BCH2(YKR027W)|FD-Score:4.52|P-value:3.07E-6||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BUD16(YEL029C)|FD-Score:7.24|P-value:2.24E-13||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:BUD25(YER014C-A)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:CCP1(YKR066C)|FD-Score:4.5|P-value:3.32E-6||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CCS1(YMR038C)|FD-Score:4.5|P-value:3.42E-6||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CPR8(YNR028W)|FD-Score:3.75|P-value:8.93E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:CTI6(YPL181W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:DAL7(YIR031C)|FD-Score:4.01|P-value:3.00E-5||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:ECM4(YKR076W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ELP6(YMR312W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERG3(YLR056W)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERI1(YPL096C-A)|FD-Score:5.62|P-value:9.48E-9||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:EXG1(YLR300W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:FAP1(YNL023C)|FD-Score:4.49|P-value:3.56E-6||SGD DESC:Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 Gene:HEM14(YER014W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HFI1(YPL254W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HXT17(YNR072W)|FD-Score:4.53|P-value:2.91E-6||SGD DESC:Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose Gene:ICP55(YER078C)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family Gene:IRC19(YLL033W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci Gene:MAC1(YMR021C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDM38(YOL027C)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MOG1(YJR074W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MRPL10(YNL284C)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:MRPS8(YMR158W)|FD-Score:4.87|P-value:5.47E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSH1(YHR120W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MTG2(YHR168W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly Gene:MUS81(YDR386W)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:OAZ1(YPL052W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:PDH1(YPR002W)|FD-Score:5.27|P-value:6.96E-8||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PET100(YDR079W)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PRE9(YGR135W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:PRS3(YHL011C)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RAD52(YML032C)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:RPL22B(YFL034C-A)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPS1B(YML063W)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication Gene:RTC6(YPL183W-A)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SAL1(YNL083W)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:ADP/ATP transporter; member of the Ca2+-binding subfamily of mitochondrial carriers, with two EF-hand motifs; transport activity of either Sal1p or Pet9p is critical for viability; polymorphic in different S. cerevisiae strains Gene:SNA3(YJL151C)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles Gene:SNF2(YOR290C)|FD-Score:-3.34|P-value:4.18E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SOD1(YJR104C)|FD-Score:6.21|P-value:2.68E-10||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SRL2(YLR082C)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation Gene:STD1(YOR047C)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:TOS1(YBR162C)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TSC3(YBR058C-A)|FD-Score:23.5|P-value:4.20E-122||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:4.29|P-value:8.95E-6||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VAC17(YCL063W)|FD-Score:5.96|P-value:1.23E-9||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VPS3(YDR495C)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS45(YGL095C)|FD-Score:7.02|P-value:1.12E-12||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBR113W(YBR113W_d)|FD-Score:4.29|P-value:8.98E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YBR174C(YBR174C_d)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR300C(YBR300C_d)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YCL074W(YCL074W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDR271C(YDR271C_d)|FD-Score:-3.57|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YEA6(YEL006W)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YGL185C(YGL185C_p)|FD-Score:3.3|P-value:4.92E-4||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR051C(YGR051C_d)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Gene:YGR111W(YGR111W_p)|FD-Score:-3.18|P-value:7.28E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YIL067C(YIL067C_p)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YJL163C(YJL163C_p)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Putative protein of unknown function Gene:YMC1(YPR058W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC1 has a paralog, YMC2, that arose from the whole genome duplication Gene:ZAP1(YJL056C)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR282W_d5.334.89E-80.64YOR282W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
YNL240C4.943.97E-70.64NAR1Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf
YLL031C4.308.46E-60.17GPI13ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein
YGR083C4.131.83E-50.09GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YOR260W4.042.65E-50.21GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YOR146W_d3.836.32E-50.03YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YIR011C3.817.06E-50.27STS1Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation
YJR057W3.542.03E-40.02CDC8Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p
YDR413C_d3.522.16E-40.09YDR413C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing
YKL195W3.432.96E-40.12MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YMR049C3.314.63E-40.01ERB1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1
YDL164C3.304.85E-40.27CDC9DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination
YGR172C3.030.001210.13YIP1Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4
YLR033W2.910.001830.12RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YFR052W2.780.002690.07RPN12Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A23.504.20E-122TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YEL029C7.242.24E-13BUD16Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK)
YGL095C7.021.12E-12VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YPL078C6.426.95E-11ATP4Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated
YJR104C6.212.68E-10SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YCL063W5.961.23E-9VAC17Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p
YPL096C-A5.629.48E-9ERI1Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein
YPR002W5.276.96E-8PDH1Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate
YMR158W4.875.47E-7MRPS8Mitochondrial ribosomal protein of the small subunit
YNR072W4.532.91E-6HXT17Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose
YKR027W4.523.07E-6BCH2Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication
YKR066C4.503.32E-6CCP1Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress
YMR038C4.503.42E-6CCS1Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress
YNL023C4.493.56E-6FAP1Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1
YML124C4.298.95E-6TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1891
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4800SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.0857143TSC3-RPN4
0.4640SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0350877TSC3-RPN4
0.4451.19E-285SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0666667TSC3-RPN4
0.4362.40E-272SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.15TSC3-RPN4
0.4183.62E-248SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.150943TSC3-RPN4
0.3971.74E-222SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0961538TSC3-RPN4
0.3885.45E-211SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.175439TSC3-RPN4
0.3821.64E-204SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.173077TSC3-RPN4
0.3782.63E-200SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.138462
0.3692.69E-190SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.19697TSC3-RPN4
0.3661.72E-186SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0535714TSC3-RPN4
0.3652.00E-185SGTC_1813st051246 8.6 μMTimTec (Natural product derivative library)52919590.0727273TSC3-RPN4
0.3553.55E-175SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0526316TSC3-RPN4
0.3559.19E-175SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.0925926TSC3-RPN4
0.3532.56E-172SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.0714286

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_12670828-02729.49 μM0.347826745334ChemDiv (Drug-like library)278.301963.72403
SGTC_1913st06021396.1 μM0.347826688959TimTec (Natural product derivative library)208.1857632.14204
SGTC_20024023503131 μM0.3255814736863Chembridge (Fragment library)193.1744231.4303mitochondrial processes
SGTC_13271436-00822.18 μM0.31914915992205ChemDiv (Drug-like library)329.144863.503
SGTC_20605238662140 μM0.317073790130Chembridge (Fragment library)195.1472430.5114
SGTC_169k015-001716.03 μM0.2857141840002ChemDiv (Drug-like library)334.1398633.41303
SGTC_1751st04483927.19 μM0.2758625291326TimTec (Natural product derivative library)291.32232-1.08426Golgi
SGTC_1898st06019918.6 μM0.270833688866TimTec (Natural product derivative library)219.623842.37703
SGTC_12680828-02752.46 μM0.265306745337ChemDiv (Drug-like library)284.693863.41603
SGTC_1975st07096730.8 μM0.26087261391TimTec (Natural product derivative library)240.2291633.34103