st060216

4-methoxy-3,6-dimethylchromen-2-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1901
Screen concentration 58.6 μM
Source TimTec (Natural product derivative library)
PubChem CID 688792
SMILES CC1=CC2=C(C=C1)OC(=O)C(=C2OC)C
Standardized SMILES COC1=C(C)C(=O)Oc2ccc(C)cc12
Molecular weight 204.2219
ALogP 2.52
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 67.21
% growth inhibition (Hom. pool) 4.86


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 688792
Download HIP data (tab-delimited text)  (excel)
Gene:BET1(YIL004C)|FD-Score:3.96|P-value:3.72E-5|Clearance:0.19||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:CDS1(YBR029C)|FD-Score:3.28|P-value:5.10E-4|Clearance:0.29||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:KOG1(YHR186C)|FD-Score:-4.41|P-value:5.23E-6|Clearance:0||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:MED6(YHR058C)|FD-Score:-3.18|P-value:7.25E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:RFT1(YBL020W)|FD-Score:3.37|P-value:3.77E-4|Clearance:0.01||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RNA1(YMR235C)|FD-Score:-3.58|P-value:1.69E-4|Clearance:0||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:RPS20(YHL015W)|FD-Score:4.43|P-value:4.72E-6|Clearance:0.32||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS5(YJR123W)|FD-Score:3.77|P-value:8.18E-5|Clearance:0.3||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RRB1(YMR131C)|FD-Score:-3.13|P-value:8.82E-4|Clearance:0||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:SAD1(YFR005C)|FD-Score:-3.15|P-value:8.29E-4|Clearance:0||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SLD3(YGL113W)|FD-Score:-3.14|P-value:8.52E-4|Clearance:0||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPC105(YGL093W)|FD-Score:-4.09|P-value:2.15E-5|Clearance:0||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SSY5(YJL156C)|FD-Score:-3.99|P-value:3.31E-5|Clearance:0||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:SUA5(YGL169W)|FD-Score:4.11|P-value:1.98E-5|Clearance:0.15||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TFC6(YDR362C)|FD-Score:3.36|P-value:3.92E-4|Clearance:0.07||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TIF35(YDR429C)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.1||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM17(YJL143W)|FD-Score:5.49|P-value:2.05E-8|Clearance:1.06||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:YTH1(YPR107C)|FD-Score:-4.83|P-value:6.82E-7|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:BET1(YIL004C)|FD-Score:3.96|P-value:3.72E-5|Clearance:0.19||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:CDS1(YBR029C)|FD-Score:3.28|P-value:5.10E-4|Clearance:0.29||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:KOG1(YHR186C)|FD-Score:-4.41|P-value:5.23E-6|Clearance:0||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:MED6(YHR058C)|FD-Score:-3.18|P-value:7.25E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:RFT1(YBL020W)|FD-Score:3.37|P-value:3.77E-4|Clearance:0.01||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RNA1(YMR235C)|FD-Score:-3.58|P-value:1.69E-4|Clearance:0||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:RPS20(YHL015W)|FD-Score:4.43|P-value:4.72E-6|Clearance:0.32||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS5(YJR123W)|FD-Score:3.77|P-value:8.18E-5|Clearance:0.3||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RRB1(YMR131C)|FD-Score:-3.13|P-value:8.82E-4|Clearance:0||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:SAD1(YFR005C)|FD-Score:-3.15|P-value:8.29E-4|Clearance:0||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SLD3(YGL113W)|FD-Score:-3.14|P-value:8.52E-4|Clearance:0||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPC105(YGL093W)|FD-Score:-4.09|P-value:2.15E-5|Clearance:0||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SSY5(YJL156C)|FD-Score:-3.99|P-value:3.31E-5|Clearance:0||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:SUA5(YGL169W)|FD-Score:4.11|P-value:1.98E-5|Clearance:0.15||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TFC6(YDR362C)|FD-Score:3.36|P-value:3.92E-4|Clearance:0.07||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TIF35(YDR429C)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.1||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM17(YJL143W)|FD-Score:5.49|P-value:2.05E-8|Clearance:1.06||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:YTH1(YPR107C)|FD-Score:-4.83|P-value:6.82E-7|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 688792
Download HOP data (tab-delimited text)  (excel)
Gene:ADE1(YAR015W)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:AFT1(YGL071W)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM25(YJR100C)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:AIM36(YMR157C)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:APL1(YJR005W)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:APL4(YPR029C)|FD-Score:-3.19|P-value:7.24E-4||SGD DESC:Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport Gene:BAG7(YOR134W)|FD-Score:6.24|P-value:2.24E-10||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:BNI4(YNL233W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:COX12(YLR038C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:CSF1(YLR087C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CUE1(YMR264W)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:ECI1(YLR284C)|FD-Score:5.56|P-value:1.33E-8||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:FLC2(YAL053W)|FD-Score:-3.09|P-value:9.84E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FMP52(YER004W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GBP2(YCL011C)|FD-Score:5.56|P-value:1.35E-8||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GLE2(YER107C)|FD-Score:4.97|P-value:3.40E-7||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HEF3(YNL014W)|FD-Score:4.29|P-value:8.89E-6||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:IDH2(YOR136W)|FD-Score:5.57|P-value:1.25E-8||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IML2(YJL082W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:INP2(YMR163C)|FD-Score:4.59|P-value:2.21E-6||SGD DESC:Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene Gene:IRC13(YOR235W_d)|FD-Score:5.5|P-value:1.87E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:LPP1(YDR503C)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA Gene:MCR1(YKL150W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MHO1(YJR008W)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility Gene:MRPL40(YPL173W)|FD-Score:4.81|P-value:7.55E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:6.05|P-value:7.40E-10||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MSA2(YKR077W)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSC7(YHR039C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:NUP2(YLR335W)|FD-Score:3.78|P-value:7.89E-5||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:OAZ1(YPL052W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:ODC1(YPL134C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation Gene:OST6(YML019W)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:OTU2(YHL013C)|FD-Score:-3.15|P-value:8.24E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PDR8(YLR266C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PFK1(YGR240C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHO85(YPL031C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:POL32(YJR043C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PSP2(YML017W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:ROT2(YBR229C)|FD-Score:6.39|P-value:8.17E-11||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPL2A(YFR031C-A)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RPL40A(YIL148W)|FD-Score:6.43|P-value:6.31E-11||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS21B(YJL136C)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RRT5(YFR032C_p)|FD-Score:-4.2|P-value:1.34E-5||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:RTK1(YDL025C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SCS7(YMR272C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SEC72(YLR292C)|FD-Score:4.44|P-value:4.44E-6||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SYF2(YGR129W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TAN1(YGL232W)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TCB2(YNL087W)|FD-Score:5.54|P-value:1.50E-8||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:THR4(YCR053W)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TOP1(YOL006C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TRM9(YML014W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TRP1(YDR007W)|FD-Score:3.88|P-value:5.12E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TSR2(YLR435W)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:ULA1(YPL003W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:VAC17(YCL063W)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VOA1(YGR106C)|FD-Score:4.28|P-value:9.38E-6||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS1(YKR001C)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS45(YGL095C)|FD-Score:6.83|P-value:4.27E-12||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBP1(YBR216C)|FD-Score:-5.05|P-value:2.17E-7||SGD DESC:Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication Gene:YBR206W(YBR206W_d)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YCL002C(YCL002C_p)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER034W(YER034W_p)|FD-Score:5|P-value:2.82E-7||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YET1(YKL065C)|FD-Score:-3.65|P-value:1.34E-4||SGD DESC:Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YGR168C(YGR168C_p)|FD-Score:5.46|P-value:2.38E-8||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YIL002W-A(YIL002W-A_p)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL160C(YJL160C_p)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YJR107W(YJR107W_p)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Putative lipase Gene:YKL115C(YKL115C_d)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YKL118W(YKL118W_d)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YOR342C(YOR342C_p)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YOR385W(YOR385W_p)|FD-Score:-3.87|P-value:5.34E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL113C(YPL113C_p)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene Gene:YPS6(YIR039C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance Gene:YPT52(YKR014C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:ADE1(YAR015W)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:AFT1(YGL071W)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM25(YJR100C)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:AIM36(YMR157C)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:APL1(YJR005W)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:APL4(YPR029C)|FD-Score:-3.19|P-value:7.24E-4||SGD DESC:Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport Gene:BAG7(YOR134W)|FD-Score:6.24|P-value:2.24E-10||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:BNI4(YNL233W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:COX12(YLR038C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:CSF1(YLR087C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CUE1(YMR264W)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:ECI1(YLR284C)|FD-Score:5.56|P-value:1.33E-8||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:FLC2(YAL053W)|FD-Score:-3.09|P-value:9.84E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FMP52(YER004W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GBP2(YCL011C)|FD-Score:5.56|P-value:1.35E-8||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GLE2(YER107C)|FD-Score:4.97|P-value:3.40E-7||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HEF3(YNL014W)|FD-Score:4.29|P-value:8.89E-6||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:IDH2(YOR136W)|FD-Score:5.57|P-value:1.25E-8||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IML2(YJL082W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:INP2(YMR163C)|FD-Score:4.59|P-value:2.21E-6||SGD DESC:Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene Gene:IRC13(YOR235W_d)|FD-Score:5.5|P-value:1.87E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:LPP1(YDR503C)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA Gene:MCR1(YKL150W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MHO1(YJR008W)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility Gene:MRPL40(YPL173W)|FD-Score:4.81|P-value:7.55E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:6.05|P-value:7.40E-10||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MSA2(YKR077W)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSC7(YHR039C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:NUP2(YLR335W)|FD-Score:3.78|P-value:7.89E-5||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:OAZ1(YPL052W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:ODC1(YPL134C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation Gene:OST6(YML019W)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:OTU2(YHL013C)|FD-Score:-3.15|P-value:8.24E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PDR8(YLR266C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PFK1(YGR240C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHO85(YPL031C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:POL32(YJR043C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PSP2(YML017W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:ROT2(YBR229C)|FD-Score:6.39|P-value:8.17E-11||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPL2A(YFR031C-A)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RPL40A(YIL148W)|FD-Score:6.43|P-value:6.31E-11||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS21B(YJL136C)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RRT5(YFR032C_p)|FD-Score:-4.2|P-value:1.34E-5||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:RTK1(YDL025C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SCS7(YMR272C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SEC72(YLR292C)|FD-Score:4.44|P-value:4.44E-6||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SYF2(YGR129W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TAN1(YGL232W)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TCB2(YNL087W)|FD-Score:5.54|P-value:1.50E-8||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:THR4(YCR053W)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TOP1(YOL006C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TRM9(YML014W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TRP1(YDR007W)|FD-Score:3.88|P-value:5.12E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TSR2(YLR435W)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:ULA1(YPL003W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:VAC17(YCL063W)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VOA1(YGR106C)|FD-Score:4.28|P-value:9.38E-6||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS1(YKR001C)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS45(YGL095C)|FD-Score:6.83|P-value:4.27E-12||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBP1(YBR216C)|FD-Score:-5.05|P-value:2.17E-7||SGD DESC:Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication Gene:YBR206W(YBR206W_d)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YCL002C(YCL002C_p)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER034W(YER034W_p)|FD-Score:5|P-value:2.82E-7||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YET1(YKL065C)|FD-Score:-3.65|P-value:1.34E-4||SGD DESC:Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YGR168C(YGR168C_p)|FD-Score:5.46|P-value:2.38E-8||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YIL002W-A(YIL002W-A_p)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL160C(YJL160C_p)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YJR107W(YJR107W_p)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Putative lipase Gene:YKL115C(YKL115C_d)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YKL118W(YKL118W_d)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YOR342C(YOR342C_p)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YOR385W(YOR385W_p)|FD-Score:-3.87|P-value:5.34E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL113C(YPL113C_p)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene Gene:YPS6(YIR039C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance Gene:YPT52(YKR014C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL143W5.492.05E-81.06TIM17Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
YHL015W4.434.72E-60.32RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
YGL169W4.111.98E-50.15SUA5Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family
YIL004C3.963.72E-50.19BET1Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins
YJR123W3.778.18E-50.30RPS5Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7
YDR429C3.472.59E-40.10TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YBL020W3.373.77E-40.01RFT1Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein
YDR362C3.363.92E-40.07TFC6One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
YBR029C3.285.10E-40.29CDS1Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids
YAL034W-A3.000.001370.04MTW1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly
YGR114C_d2.950.001580.05YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YGL044C2.900.001880.01RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YOL077C2.890.001940.05BRX1Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif
YOR244W2.840.002270.02ESA1Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy
YOR294W2.820.002410.05RRS1Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL095C6.834.27E-12VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YIL148W6.436.31E-11RPL40AUbiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YBR229C6.398.17E-11ROT2Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
YOR134W6.242.24E-10BAG7Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p
YNR036C6.057.40E-10MRPS12Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins
YOR136W5.571.25E-8IDH2Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated
YLR284C5.561.33E-8ECI1Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced
YCL011C5.561.35E-8GBP2Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication
YNL087W5.541.50E-8TCB2ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud
YOR235W_d5.501.87E-8IRC13_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci
YGR168C_p5.462.38E-8YGR168C_pPutative protein of unknown function; YGR168C is not an essential gene
YER034W_p5.002.82E-7YER034W_pProtein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress
YER107C4.973.40E-7GLE2RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
YPL173W4.817.55E-7MRPL40Mitochondrial ribosomal protein of the large subunit
YMR163C4.592.21E-6INP2Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene

GO enrichment analysis for SGTC_1901
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1605.08E-35SGTC_1902st060212 70.5 μMTimTec (Natural product derivative library)6889440.340426
0.1471.06E-29SGTC_1900st060200 72.8 μMTimTec (Natural product derivative library)6889450.3125
0.1462.67E-29SGTC_1904st060225 78.0 μMTimTec (Natural product derivative library)665950.26
0.0921.72E-12SGTC_14740330-0125 5.3 μMChemDiv (Drug-like library)X14740.0923077
0.0881.29E-11SGTC_12790874-0590 65.2 μMChemDiv (Drug-like library)35230740.103448
0.0791.42E-9SGTC_10410206-0046 107.0 μMChemDiv (Drug-like library)504940.222222
0.0782.34E-9SGTC_21986589944 200.0 μMChembridge (Fragment library)52902680.192982
0.0765.65E-9SGTC_1290088-0017 176.6 μMChemDiv (Drug-like library)1824460.101695
0.0766.32E-9SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.157895
0.0732.15E-8SGTC_5423381-0244 89.3 μMChemDiv (Drug-like library)11128430.140625
0.0714.14E-8SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.156863
0.0681.78E-7SGTC_2540epiandrosterone 100.0 μMTimTec (Pure natural product library)4413020.0625
0.0681.89E-7SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.296296
0.0681.95E-7SGTC_790052-0007 94.1 μMChemDiv (Drug-like library)28014720.0877193
0.0664.58E-7SGTC_6383970-0837 4.1 μMChemDiv (Drug-like library)41184510.133333

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1907st06021083.7 μM0.431818688793TimTec (Natural product derivative library)204.221882.42203
SGTC_1732st03430851.82 μM0.418605830119TimTec (Natural product derivative library)277.531083.63602
SGTC_15526-methylflavone10.6 μM0.409091689013TimTec (Pure natural product library)236.265283.62202superoxide
SGTC_1816st05195791.6 μM0.372093674121TimTec (Natural product derivative library)218.248463.26203
SGTC_1865st05763952.2 μM0.367347688818TimTec (Natural product derivative library)312.316642.79105
SGTC_26222-methoxyxanthone59.94 μM0.36363671034Microsource (Natural product library)226.22742.94803
SGTC_3088911757849.47 μM0.355556678341Chembridge (Drug-like library)251.279924.11303amide catabolism
SGTC_1782st03942380.64 μM0.3469392068169TimTec (Natural product derivative library)246.301624.10403
SGTC_1902st06021270.5 μM0.340426688944TimTec (Natural product derivative library)204.221882.42203
SGTC_1868st05764442.7 μM0.34688665TimTec (Natural product derivative library)282.290662.80804